• Journal of Pharmaceutical Investigation
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• v.16 no.4
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• pp.139-147
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• 1986

A singly charged methyl orange(MO) anion was found to be extracted with chlorpheniramine maleate(CPM) as a 1 : 1 complex in chloroform. Of various solvents, MO-chloroform system gave a yellow color for CPM, while in the absence of CPM, an organic phase showed almost no color. In this way, the spectrophotometric method was investigated for the determination of CPM by solvent extraction. The addition of alcoholic bolic acid solution to the solvent extract gave a higher color stability and transparency at least 5 days, but the extract alone lost its color intensity significantly. CPM is determined by measuring the absorbance of the extracts over a range of $1{\sim}7{\times}10^{-4}M\;(39{\sim}273\;{\mu}g/ml)$ in aqueous solution at 423 nm. The molar absorptivity was $2.26{\times}10^3\;l,\;mol^{-1},\;cm^{-1}$. The absorbance of the extract was constant in the range of pH $3.7{\sim}4.6$. This novel method was applied for the determination of CPM in artificial and commercial preparations in comparison with the analytical method of CPM tablets in K.P.IV. The results obtained showed that the former was better in accuracy and time consumption than the latter.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.7
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• pp.902-907
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• 2007

This study was carried out to find the effect of ${\gamma}$ -irradiation on the physical properties of alginic acid and ${\lambda}$-carrageenan solution. Alginic acid and ${\lambda}$-carrageenan aqueous solution were irradiated with $Co^{60}$ ${\gamma}$ -rays in dosages ranging from 3 to 100 kGy, and then the molecular weight, viscosity and color were measured. The molecular weight of alginic acid and ${\lambda}$-carrageenan solution were decreased as the ${\gamma}$ -irradiation dosage increased. In addition, the viscosity of irradiated alginic acid and ${\lambda}$-carrageenan solution were decreased depending upon the ${\gamma}$ -irradiation dosage, too. The high dosage irradiated alginic acid and ${\lambda}$-carrageenan solution's color were changed to yellow. These results suggest that low dosage of ${\gamma}$ -irradiation from 3 to 20 kGy is a very effective method for producing low-molecular alginic acid and ${\lambda}$-carrageenan.

• Preventive Nutrition and Food Science
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• v.17 no.3
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• pp.184-191
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• 2012

The purpose of this study was to investigate the fatty acid profiles in 18 soybean cultivars grown in Korea. A total of eleven fatty acids were identified in the sample set, which was comprised of myristic (C14:0), palmitic (C16:0), palmitoleic (C16:1, ${\omega}7$), stearic (C18:0), oleic (C18:1, ${\omega}9$), linoleic (C18:2, ${\omega}6$), linolenic (C18:3, ${\omega}3$), arachidic (C20:0), gondoic (C20:1, ${\omega}9$), behenic (C22:0), and lignoceric (C24:0) acids by gas-liquid chromatography with flame ionization detector (GC-FID). Based on their color, yellow-, black-, brown-, and green-colored cultivars were denoted. Correlation coefficients (r) between the nine major fatty acids identified (two trace fatty acids, myristic and palmitoleic, were not included in the study) were generated and revealed an inverse association between oleic and linoleic acids (r=-0.94, p<0.05), while stearic acid was positively correlated to arachidic acid (r=0.72, p<0.05). Principal component analysis (PCA) of the fatty acid data yielded four significant principal components (PCs; i.e., eigenvalues>1), which together account for 81.49% of the total variance in the data set; with PC1 contributing 28.16% of the total. Eigen analysis of the correlation matrix loadings of the four significant PCs revealed that PC1 was mainly contributed to by oleic, linoleic, and gondoic acids, PC2 by stearic, linolenic and arachidic acids, PC3 by behenic and lignoceric acids, and PC4 by palmitic acid. The score plots generated between PC1-PC2 and PC3-PC4 segregated soybean cultivars based on fatty acid composition.

• Journal of the Korean Wood Science and Technology
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• v.41 no.4
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• pp.269-275
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• 2013

Antifungal and insecticidal activity of Korean traditional medicinal plants was carried out to develop natural material for the development of organic cultural heritage conservation. As a result, Phellodendron amurense fruit was finally selected as a candidate of antifungal and insecticidal natural material. An novel active compound was purified from the ethylacetate fraction of Phellodendron amurense fruits using silica gel and Sephadex LH-20 column chromatography and PTLC. The compound was obtained as yellow oil form; UV ${\lambda}_{max}$(MeOH): 260 nm. The chemical structure of novel compound was determined as (4'-ethyl-2'-methylfuranyl)-6-methoxy-7-methylnona-2E,4E,6Z,8E-tetraenoic acid on the basis of various NMR experiments including $^1H$- and $^{13}C$-NMR, HMQC, HMBC and ESI-mass spectrum.

• Journal of the Korean Society of Food Science and Nutrition
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• v.17 no.3
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• pp.211-214
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• 1988

The thermal drying of chestnut powder showed a distinct discoloration from yellow to reddish brown color and resulted in a decrease in lysine content of chestnut protein. Chanted powders were blanched in hot solutions of 0.1% sodium bisulfate, 0.2% alum, 0.05% vitamin C and 0.1% citric acid and dried for 7 days in $50^{\cire}C$ of heatine drier. The color difference values in contrast with the original chestnut powder were 1.05, 1.24, 3.47 and 3.34, respectively. In particular, the effect of 0.1% potassium metabisulfide was distinguished. Under the blanching conditions used, a blanching period of 3 min resulted in the most favorable color of chestnut powder.

• KSBB Journal
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• v.20 no.1 s.90
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• pp.66-70
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• 2005

Optimization of culture conditions for pigment production of Monascus purpureus P-57 mutant was investigated in liquid culture. The optimum composition of medium for the pigment production was $4\%$ rice power, $0.1\%$ beef extract, $0.03\%$ glutamic acid, $0.1\%\;MgSO_4{\cdot}7H_2O,\;0.25\%\;KH_2PO_4$, the optimum initial pH was 5.0. And the optimum culture conditions was at $30^{\circ}C$ for 8 days under 150 rpm with shaking. M. purpureus P-57 mutant produced the highest pigment as 356.04 units at red pigment and as 268.20 units at yellow pigment, and produced high cell mass as 15.00 g/L in liquid culture under the optimum conditions.

• Journal of the Korean Society of Floral Art and Design
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• no.42
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• pp.23-35
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• 2020

Standard carnations are widely used in flower design as a mass flower, but there is a limit to the use in that it can not be used in various colors in addition to its own color. The purpose of this study was to investigate how does artificial dyes affect vase life, using standard carnations, and to improve utilization of dyeing carnations in floral design. Using standard carnation 'White Liberty', dyeing experiment was performed according to four kinds of chemicals for each of six dyes. Six different dyes from Koch(Robert Koch industries Inc., USA) as follows light blue(2386), lime green(2315), christmas red(2506), lavender(2200), orange fire(2268) and black(2012) have been used and four different chemicals as follows distilled water, 4% ethanol, 3% sucrose and 100mg·L^-1 citric acid have been used with the cut Dianthus caryophyllus 'White Liberty'. As a result, six different dyes showed fast and excellent dyeing with 3% sucrose and 100mg·L^-1 citric acid treatment. But vase life in other dyes except black and lavender tended to be similar to control(7 days).

• Korean Journal of Agricultural Science
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• v.34 no.2
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• pp.99-109
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• 2007

Cellulomanas sp. CS 1-1 was studied for its morphological, physiological and biochemical characteristics, together with DNA homology and fatty acid pattern to elucidate its taxonomical position in the species level. Colony morphology of CS1-1 exhibited circular form, opaque, convex, entire edge and pale yellow. Cells were of rod with the size of $0.3{\sim}0.5{\times}0.8{\sim}1.2{\mu}m$, while coryneforms were formed at the early stage of culture. D-ribose, raffinose, rhamnose, acetate, propionate, L-lactate, D-gluconate, aspartate and proline were not utilized as a sole source of carbon, whereas saccharose, arabinose, and amlyose were utilized. Biochemical characteristics of CS1-1 were Gram positive, catalase positive, oxidase negative, nonmotile, facultative anaerobic, mesophilic and G+C content of 74.7 mol %. The major fatty acid and menaquinone were 12-methyltetradecanoic acid(anteiso-$C_{15:1}$) and MK-$9(H_4)$, respectively. These results were correspondent with the characteristics reported for member of the genus Cellulomonas. The strain CS 1-1 exhibited a high level of DNA homology as 70% with C. uda ATCC491, compared to those of 54~59% with C. fimi ATCC 15724, 46~48% with C. biazotea, C. gelida and C. bibula. Finally, strain CS1-1 could be classified as a novel species belongs to C. uda.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.3
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• pp.229-236
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• 2008

This experiment was conducted to study on the evaluation of fermentation ability of microbes for whole crop rice silage Inoculant at National Institute of Animal Science, RDA from 2004 to 2005. We collected 28 strains of microbes from whole crop rice silage. According to acidity and growth ability, 5 strains of microbes was isolated (R4-1, R7-1, R7-2, R10-1, R12-1). The cultures of 4 strains were identified to be Lactobacillus plantarum (R4-1, R7-1, R7-2 and R10-1) and one was identified to be Lactobacillus pentosus (R12-1). Whole crop rice was harvested at the yellow ripen stage. It was ensiled in experimental silos (20ℓ capacity) with or without microbial additives (R4-1, R7-1, R7-2, R10-1, R12-1 and three commercial inoculant) and stored at room temperature for 60d. The pH value and acetic acid content of additivetreated silages were lower and lactic acid content was higher than those of the control (p<0.05). There was a trend for acetic acid content to be lowest and lactic acid to be highest in R7-1 treated silage. Crude protein (CP) contents of R7-2 treated silage was higher and acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents of R7-1 treated silage was lower (p<0.05). Although some strains of inoculant could improve silage quality, L. plantarum R7-1 was more effective as an inoculant for whole crop rice silage. This microbe was named NLRI 401 and registered in the Korea Agricultural Culture Collection.

• International Journal of Industrial Entomology and Biomaterials
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• v.25 no.2
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• pp.209-213
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• 2012

The propolis was collected from the whole part of Korea Peninsula, such as central, southern, and Jeju island, to analyze the general composition including total flavonoid and phenolic contents, heavy metals, colors, amino acids, and crude lipid. The total flavonoid and phenolic contents of middle region in Korea Peninsula (4.26% and 13.06 g/100 g gallic acid) were higher than southern region (2.53%, 10.9 g/100 g gallic acid) and Jeju island (0.03%, 7.22g/100 g gallic acid), respectively. The heavy metals contents showed that Zn contents were found in $0.44{\pm}0.2ppm$ and a harmful heavy metal such as Cr, Cd, Pb, Ni, Cu, As, and Hg were not detected at all in our experiment. The colors of extracted propolis showed a various spectrum from yellow to red purple. Various amino acids were also detected as proline, valine, methionine and the average crude lipid contents of propolis were 42.4%. All data collected in South Korea were very first executed in our institution for the purposes of utilizing and developing the industrial insects, honeybees as well as silkworm.