A New Compound Isolation and Structure Analysis from Phellodendron Amurense Fruit Extract

Antifungal and insecticidal activity of Korean traditional medicinal plants was carried out to develop natural material for the development of organic cultural heritage conservation. As a result, Phellodendron amurense fruit was finally selected as a candidate of antifungal and insecticidal natural material. An novel active compound was purified from the ethylacetate fraction of Phellodendron amurense fruits using silica gel and Sephadex LH-20 column chromatography and PTLC. The compound was obtained as yellow oil form; UV ${\lambda}_{max}$(MeOH): 260 nm. The chemical structure of novel compound was determined as (4'-ethyl-2'-methylfuranyl)-6-methoxy-7-methylnona-2E,4E,6Z,8E-tetraenoic acid on the basis of various NMR experiments including $^1H$- and $^{13}C$-NMR, HMQC, HMBC and ESI-mass spectrum.

황벽나무 열매 추출물로부터 신규 화합물의 분리 및 구조분석

본 연구는 유기질 문화재 보존을 위해 한국 전통 약용식물을 대상으로 항균 및 살충활성을 검증한 결과 황벽나무 열매 추출물이 우수한 활성을 나타냄을 확인하였다. 황벽나무 열매 추출물을 비극성 용매로 분획하여 그 중 가장 활성이 우수한 ethylacetate 분획물을 대상으로 실리카젤 및 Sephadex LH-20 컬럼 크로마토그래피를 실시하고 Prep-TLC를 이용하여 화합물을 순수 분리하였다. 화합물은 UV spectrum 분석 결과 260 nm 파장에서 최대흡수피크가 관찰되었고, $^1H$, $^{13}C$, HMQC, HMBC NMR과 ESI mass spectrum을 이용하여 화학구조를 분석한 결과 (4'-ethyl-2'-methylfuranyl)-6-methoxy-7-methylnona-2E,4E,6Z,8E-tetraenoic acid의 새로운 화합물임을 알 수 있었다.