• Horticultural Science & Technology
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• v.28 no.6
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• pp.1003-1013
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• 2010

Fifty-nine accessions of 23 species in genus Rosa were collected, and 15 accessions of Rosa rugosa were collected throughout 10 regions of Korea. Their genetic relationship was investigated by using morphological analysis and RAPD marker. The morphological analysis was measured for 7 quantitative and quantified for 4 qualitative traits. RAPD analysis obtained a total of 959 polymorphic bands by using twenty primers. Morphological analysis classified most according to the rose section system except for several species. The cluster analysis of genus Rosa based on RAPD data could identify the subgenus $Platyrhodon$ and $Eurosa$. The subgenus $Eurosa$ separated five sections; $Gallicanae$, $Cinnamomeae$, $Pimpinellifoliae$, $Synstylae$ and $Caninae$. Correlation analysis between morphological and RAPD analysis showed low significance ($r$ = 0.35). The accessions of R. rugosa belonged to the section $Cinnamomeae$ clustered into three groups at genetic distance ranging from 0.28 on the base of RAPD analysis. In conclusion, the genetic relationship of the genus Rosa was consistent to the previously reported rose section system, and domestic collections of $R.$ $rugosa$ were separated from 3 groups on the base of RAPD marker.

• Korean Journal of Plant Resources
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• v.16 no.2
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• pp.123-129
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• 2003

This study was conducted to determine the germination characteristics, oil contents and fatty acid compositions of the different safflower accessions. The safflower accessions had nearly the same germination period of about 12-13 days and the germination rate was more than 87.5%. Plant height grew until 84 days after seeding. The number of leaves did not increase after 70 days from seeding. The accessions had a difference of six loaves from 17 to 23 loaves. Flowering time had a difference of 11 days from 79 days after planting to 90 days after planting. The Jangsung accession had the shortest flowering time of 79 days after planting. In the characteristics of bearing fruit, the number of branches including main stem are from 4.7 to 8.8. The number of head flowers had nearly the same number of branches which were 4.8-8.9, numbers of seed per head flower were 29.3-49.1 and the weight of 100 seeds were 2.9-3.9 g. The crude fat content was 21.8-33.9％ and fatty acid content was 66.6-77.6% containing mostly linolenic acid. Oeic acid content was 9.2-16.5% and it contained palmitic acid, stearic acid, venin acid, arachidonic acid etc. Results of this study, the accession Jangsung, which had the shortest flowering time and had the most crude fat content, was observed to be the best accession for the breeding of safflower.

• Korean Journal of Plant Resources
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• v.24 no.5
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• pp.650-658
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• 2011

The 460 collections of 60 crops were collected during 34 days from 28 November to 31 December 2008. The 295 accessions of 43 crops, 49 accessions of 24 crops and 116 accessions of 42 crops were collected in Kangwha, Ulreung and Jeju island in this exploration. Among the total collections, the portion of the food crops, vegetable or ornamental crops and industrial crops were 54.8%, 25.4% and 19.8%, respectively. The farmers conserved 48.4% to 53.6% of the landrace crops for 31 to 50 years. Furthermore, 13.1% to 18.7 % of the collections were cultivated over generation to generation. Most of the crop seeds were conserved by woman farmers aged from 61 to 80 years old. The 82.9% of the seed distributors were also woman farmers, whereas 17.1% was distributed by man farmers.

• Horticultural Science & Technology
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• v.30 no.2
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• pp.171-177
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• 2012

A total of 94 tomato accessions were evaluated for the resistance to $Tomato$ $spotted$ $wilt$ $virus$ (TSWV) using a Sw5-2 SCAR marker and bioassay. PCR products of the marker were approximately 574 bp, 500 bp, and 462 bp, among which the longest was linked to TSWV resistance allele of Sw5-b. This allele was only found in three accessions (09-438, 10-318, and 10-321) in which some individuals showed apparent recovery or stem necrosis symptom to a tomato isolate of TSWV-pb1. Thirty-five individuals (one per each accession) which were non-infected by ELISA were selected for further observation. Among these, 26 individuals that did not show any symptom at 5 months after inoculation were confirmed for viral infection by RT-PCR. TSWV-specific PCR amplicon was weakly detected in all 26 individuals including 'Eureta', a commercial F1 possessing the resistance allele of Sw5-b. The resistant genes in the selected individuals may play an important role for reducing the viral concentration in tissues of inoculated tomato plants and seems to be quantitatively controlled by several factors including Sw5-b gene.

• Weed & Turfgrass Science
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• v.2 no.3
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• pp.267-273
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• 2013

In this study, 241 zoysiagrasses (Zoysia spp.) accessions naturally growing in South Korea were collected in order to investigate the seed morphological traits. One of the goals of this study was to identify the morphological characteristics and variations of zoysiagrass seed. For the 241 zoysiagrasses accessions collected, a total of 41% (99 accessions) was interspecific hybrid zoysia, which showed various types of seed variations different from zoysiagrass species. Most of these hybrids zoysiagrasses were classified into Z. sinica type and Z. japonica type. Group I and II included Z. sinica type, this group showed 30.1 and 17.5 in number of seed per spike and 4.6 and 5.2 mm in seed length. Group III and IV included Z. japonica type, this group showed 59.2 and 45.8 in number of seed per spike and 3.3 and 3.4 mm in seed length. There is a need for additional research on growth characteristics and the molecular level for the interspecific hybridization which confirmed that genetic variation from level of gene flow between the species.

• Korean Journal of Food Science and Technology
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• v.40 no.5
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• pp.516-521
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• 2008

This study was conducted to optimize the analysis condition and quantify the 2-acetyl-1-pyrroline (2AP) in the brown aroma rice. Extraction effect of the solvent for 2AP was the order of ethanol>acetonitrile>methanol in the range from 30 to $90^{\circ}C$. In the extraction time of 15, 30, 60, and 90 min, the 30 min had the highest 2AP concentration, and it was decreased according to lapse of time. At grinding time, 5 sec resulted in highest 2AP concentration. It was recommended that five sec grinding time, using ethanol, at $90^{\circ}C$ for 30 min was the optimization conditions to quantify the 2AP. Hyangmibyeo2ho and Aranghyangchalbyeo were mild aroma. In the foreign aroma rice, 11 of 19 accessions of Indica types and 2 of 6 accessions of Japonica types were more than mild aroma. Finally, 30 accessions of aroma rice were selected based on their 2AP concentration and agronomic traits.

• Korean Journal of Agricultural Science
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• v.39 no.4
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• pp.515-523
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• 2012

Recent development and enlargement of reclaimed lands along the sea shores required to explore alternatives to existing crops, which are suitable to grow well and to declare higher profits. The objectives of this study were to investigate yield-related parameters for development of new leafy vegetables, and to screen some candidates among New Zealand spinach genotypes which were collected in Korea. Initially all the collected genotypes were grown for 7 weeks in a greenhouse of the experiment field in Chungnam National University, and then transplanted into the field to measure morphological or physiological parameters (plant height, branch number, stem diameter, chlorophyll content, and maximum canopy diameter), and yield-related parameters (leaf number, leaf length, leaf width, fresh weight and dry weight). Those parameters were quantitatively measured at 1, 5 or 9 weeks after transplanting (WAT). Parameters exhibiting statistically significant difference among the accessions were plant height, branch number, maximum canopy diameter, chlorophyll content, and leaf length. In contrast to highly correlated parameters with fresh and dry weight obtained in salt-affected soil in the earlier report, fresh weight was correlated significantly with leaf length (r=0.72), leaf width (r=0.64), and canopy diameter(r=0.66), while dry weight was correlated significantly with plant height (r=0.46), leaf length (r=0.72), leaf width (r=0.73), fresh weight (r=0.79), chlorophyll content (r=0.47), and canopy diameter (r=0.87). Based on the significantly correlated with yield parameters, the candidate accessions which were ranked in top statistical groups include CNU06A01, CNU06A13, CNU06A26, CNU06A35, CNU06A38, and CNU06A55. In order to be cultivated in reclaimed lands, it is necessary to screen out salt tolerant accessions among the above high-yielding genotypes.

• Journal of Sericultural and Entomological Science
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• v.46 no.1
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• pp.1-5
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• 2004

Rutin has many beneficial effects on human health. It was established that rutin antagonizes the increase of capillary fragility associated with hemorrhagic disease, reduces high blood pressure, decreases the permeability of the vessels, and has an antiedema effect, reduces the risk of arteriosclerosis, and shows antioxidant activity. Rutin analysis with mulberry fruits was carried out by high-performance liquid chromatography (HPLC) to reveal the possibility of source of functional food. As a result, mean content of fifty accessions was 0.14±0.050% DW. Among the tested fifty accessions, 'Sabangso' was showed the highest rutin in 0.29%DW, whereas 'Simseol' was the lowest content in 0.05% DW. Also, we researched the change of rutin content according to harvested date. The rutin contents of earlier harvested groups were higher than later harvested groups. Simultaneously, fruity characteristics as well as rutin content were researched and analyzed to select the functional mulberry varieties for the production of fruit. From the six accessions which were contained high rutin content, we selected three suitable varieties such as 'Ficus', 'Kangsun', and 'Palcheongsipyung'.

• Journal of agriculture & life science
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• v.46 no.6
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• pp.59-65
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• 2012

This study evaluated the baby-leaf growth, leaf red color intensity, and phytochemical compounds, such as total phenols and total flavonoids using domestic Amaranthus accessions, to select promising germplasm for the use of baby-leaf vegetable. The 39 accessions, upper 15% of 262 Amaranthus germplasm collection showing high red color intensity in baby-leaf, measured as Hunter's a value, were primarily selected. The eleven accessions including IT199970, showing stable red color intensity in baby-leaf both in high-temperature and low temperature cultivation, were finally selected as promising germplasm for the use of baby-leaf vegetable Amaranthus. The leaf red color intensities when they were cultivated at low temperature period were generally higher than those at low temperature period, and all the eleven selected germplasm, including four Amaranthus sub-species and five unknown species, showed stable red color expression in baby-leaf. The total phenol and total flavonoid contents were also presented for finally selected germplasm including their baby-leaf fresh weights. These finally selected germplasm could be potentially used as baby-leaf vegetable resources, through fortifying the color balance of salad vegetable.

• FLOWER RESEARCH JOURNAL
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• v.19 no.4
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• pp.231-237
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• 2011

In this study, we performed ovule culture after reciprocal crosses of two Alstroemeria accessions and investigated genetic contribution of parents by using RAPD markers. The best method was half-ovule culture on MS medium supplemented with $60g{\cdot}L^{-1}$ sucrose and $2.2g{\cdot}L^{-1}$ gelrite at 14 days after pollination. Embryos began to germinate after 6 weeks of culture. The complete plantlets were formed after 4 months of culture. In eight progenies and two parental cultivars, 59 polymorphic bands were obtained out of 89 total bands by RAPD analysis using 7 primers. Eight $F_1$ progenies from the crosses between two accessions using reciprocal crosses showed 1:1 contribution of maternal and paternal parents. It is confirmed that $F_1$ progenies were obtained from parental accessions by using RAPD markers. We conclude this cross combination showed pre-fertilization barriers with incompatibility between stigma or style, and pollen because progeny number was different in each cross combination. Thereby, it warrants overcoming pre-fertilization barrier together with post-fertilization barrier in order to broaden the heterozygosity within progeny populations in Alstroemeria breeding program.