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Genetic Relationship of Genus Rosa Germplasm and Genetic Diversity of Rosa rugosa in Korea  

Joung, Youn-Hwa (Department of Plant Biotechnology, Chonnam National University)
Kim, Seung-Tae (Floriculture Research Division, National Institute of Horticultural & Herbal Science)
Kim, Gi-Jun (Breeding Institution, Asia Seed Co. Ltd.)
Lee, Ja-Hyun (Department of Plant Biotechnology, Chonnam National University)
Gi, Gwang-Yeon (Horticultural Research Division, Jeollam-do Agricultural Research & Extension Services)
Han, Tae-Ho (Department of Plant Biotechnology, Chonnam National University)
Publication Information
Horticultural Science & Technology / v.28, no.6, 2010 , pp. 1003-1013 More about this Journal
Abstract
Fifty-nine accessions of 23 species in genus Rosa were collected, and 15 accessions of Rosa rugosa were collected throughout 10 regions of Korea. Their genetic relationship was investigated by using morphological analysis and RAPD marker. The morphological analysis was measured for 7 quantitative and quantified for 4 qualitative traits. RAPD analysis obtained a total of 959 polymorphic bands by using twenty primers. Morphological analysis classified most according to the rose section system except for several species. The cluster analysis of genus Rosa based on RAPD data could identify the subgenus $Platyrhodon$ and $Eurosa$. The subgenus $Eurosa$ separated five sections; $Gallicanae$, $Cinnamomeae$, $Pimpinellifoliae$, $Synstylae$ and $Caninae$. Correlation analysis between morphological and RAPD analysis showed low significance ($r$ = 0.35). The accessions of R. rugosa belonged to the section $Cinnamomeae$ clustered into three groups at genetic distance ranging from 0.28 on the base of RAPD analysis. In conclusion, the genetic relationship of the genus Rosa was consistent to the previously reported rose section system, and domestic collections of $R.$ $rugosa$ were separated from 3 groups on the base of RAPD marker.
Keywords
mantel test; morphological analysis; random amplified polymorphic DNA (RAPD); rose; section; subgenus;
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