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http://dx.doi.org/10.7235/hort.2012.11126

Screening of Tomato Spotted Wilt Virus Resistance in Tomato Accessions  

Han, Jung-Heon (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, College of Agriculture and Life Sciences, Seoul National University)
Choi, Hak-Soon (Vegetable Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Lee, Jun-Dae (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, College of Agriculture and Life Sciences, Seoul National University)
Kim, Jae-Deok (Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration)
Lee, Won-Phil (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, College of Agriculture and Life Sciences, Seoul National University)
Choi, Hong-Soo (Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration)
Kim, Jung-Soo (Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration)
Yoon, Jae-Bok (Research & Development Unit, Pepper & Breeding Institute, Business Incubator, College of Agriculture and Life Sciences, Seoul National University)
Publication Information
Horticultural Science & Technology / v.30, no.2, 2012 , pp. 171-177 More about this Journal
Abstract
A total of 94 tomato accessions were evaluated for the resistance to $Tomato$ $spotted$ $wilt$ $virus$ (TSWV) using a Sw5-2 SCAR marker and bioassay. PCR products of the marker were approximately 574 bp, 500 bp, and 462 bp, among which the longest was linked to TSWV resistance allele of Sw5-b. This allele was only found in three accessions (09-438, 10-318, and 10-321) in which some individuals showed apparent recovery or stem necrosis symptom to a tomato isolate of TSWV-pb1. Thirty-five individuals (one per each accession) which were non-infected by ELISA were selected for further observation. Among these, 26 individuals that did not show any symptom at 5 months after inoculation were confirmed for viral infection by RT-PCR. TSWV-specific PCR amplicon was weakly detected in all 26 individuals including 'Eureta', a commercial F1 possessing the resistance allele of Sw5-b. The resistant genes in the selected individuals may play an important role for reducing the viral concentration in tissues of inoculated tomato plants and seems to be quantitatively controlled by several factors including Sw5-b gene.
Keywords
bioassay; molecular marker; Sw5; $Tospovirus$;
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Times Cited By KSCI : 5  (Citation Analysis)  (Related Records In Web of Science)
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