Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Quantification of 2-Acetyl-1-pyrroline from the Aroma Rice Germplasm by Gas Chromatography

Gas chromatography를 이용한 향미 유전자원의 2-acetyl-1-pyrroline 정량분석

  • Kim, Jeong-Soon (Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA) ;
  • Park, One-Sung (Department of Genetic Engineering, Kyunghee University) ;
  • Ahn, Sang-Nag (Department of Agronomy, Chungnam National University) ;
  • Lee, Jung-Ro (Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA) ;
  • Gwag, Jae-Gyun (Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA) ;
  • Kim, Tae-San (Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA) ;
  • Lee, Sok-Young (Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA)
  • 김정순 (농촌진흥청 농업생명공학연구원 유전자원과) ;
  • 박원성 (경희대학교 자연과학대학 유전공학과) ;
  • 안상낙 (충남대학교 농업생명과학대학 농학과) ;
  • 이정로 (농촌진흥청 농업생명공학연구원 유전자원과) ;
  • 곽재균 (농촌진흥청 농업생명공학연구원 유전자원과) ;
  • 김태산 (농촌진흥청 농업생명공학연구원 유전자원과) ;
  • 이석영 (농촌진흥청 농업생명공학연구원 유전자원과)
  • Published : 2008.10.31
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Abstract

This study was conducted to optimize the analysis condition and quantify the 2-acetyl-1-pyrroline (2AP) in the brown aroma rice. Extraction effect of the solvent for 2AP was the order of ethanol>acetonitrile>methanol in the range from 30 to $90^{\circ}C$. In the extraction time of 15, 30, 60, and 90 min, the 30 min had the highest 2AP concentration, and it was decreased according to lapse of time. At grinding time, 5 sec resulted in highest 2AP concentration. It was recommended that five sec grinding time, using ethanol, at $90^{\circ}C$ for 30 min was the optimization conditions to quantify the 2AP. Hyangmibyeo2ho and Aranghyangchalbyeo were mild aroma. In the foreign aroma rice, 11 of 19 accessions of Indica types and 2 of 6 accessions of Japonica types were more than mild aroma. Finally, 30 accessions of aroma rice were selected based on their 2AP concentration and agronomic traits.

본 실험은 국내 육성향미자원과 유전적 배경이 서로 다른 도입향미 유전자원의 2AP 함량을 비교분석하여, 2AP 함량이 높고 농업적 형질이 우수한 자원을 선발하여 우수한 향미자원의 육성에 필요한 기초 자료를 제공하고자 실시하였다. 3종류의 유기용매에 대한 추출효율은 ethanol>acetonitle>methanol 순으로 확인되었으며, ethnanol의 경우 $30-90^{\circ}C$의 온도범위에서 3가지 유기용매 중 2AP 추출효과가 가장 뛰어났고, 특히 $90^{\circ}C$에서 30분간 추출하였을 때 2AP 추출함량이 가장 높았고 추출 시간이 경과할수록 2AP의 함량이 낮았다. 현미의 분쇄정도에 따른 2AP 추출함량은 5초간 분쇄하였을 경우 가장 높았으며 분쇄 시간이 길어질수록 낮아졌다. 선발한 30품종 중 국내 향미자원과 자포니카형 도입자원의 평균 출수일은 각각 130.3일과 131.8일로 비슷하였으며 인디카형 도입자원은 약 137.1일로 가장 늦었다. 평균 1,000립중은 인디카형 도입자원이 19.9 g으로 가장 낮았으며, 국내 향미자원이 21.65 g이었고 자포니카형 도입자원은 25.5 g으로 높았다. 선발자원의 향에 대한 관능검사는 국내 향미자원 5품종 중향남벼 2호, 아랑향찰벼가 중간 정도의 향을 띄었고 인디카형 도입자원 19품종 중에서 11품종(57.9%)과 자포니카형 도입자원 6품종 중 2품종(33.3%)이 중간 이상의 향을 나타났다. 선발한 30품종의 평균 2AP 함량은 국내 육성 향미자원이 도입 향미자원에 비해 낮았으며 도입자원에서는 인디카형이 자포니카형보다 높은 2AP 함량을 나타내었다. 국내 육성 향미자원은 아랑향찰벼외 4품종과 도입 향미자원 중 인디카형은 Basmati6313 외 18품종 및 자포니카형은 자향나 861 외 5품종을 선발하였다.

Keywords

References

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