• Korean Journal of Agricultural Science
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• v.3 no.2
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• pp.192-197
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• 1976

Study was conducted to estimate the nutritional effect of substitution levels of "IKUTON" (R) inocculate fermented sweet potato pulp for the growing-finnishing pig rations. Twenty five Large white X Landrace cross-breed (15 kg). mixed sex were used in group feeding at the substitution levels of 0, 10, 20, 30, and 40%. The results obtained were summarized as follow: Voluntary feed intake of experimental diets was lowest in 40%. No significant difference in voluntary feed intake was found in 10,20, and 30% levels except 40% P<0.05). The feed efficiency was highest in 20%; (3.43), 30%; (3.80), 10%; (3.97), control; (4.53), and 40%; (4.20) respectively. There was tendency toward increasing feed effioiency in 10,20,30% levels than control. However, the lowest feed efficiency was found in 40% level. The weeks required to gain 80 kg of live weight were 12 weeks for 10%, 13 weeks for control. 14 weeks for 20%, and 30%, but in 40% level of substitution, no 80kg live weight was found up to the end of experiment (15 weeks). Pigs in 40% level were gained live weight only 64% of control animals. The digestibility of experimental diets was 75.2-78.5%, and water intake per kg dry matter intake was $1.95-2.01{\ell}$. No significant effects of feed offer interval to the digestibility and water consumption.

• Korean Journal of Agricultural Science
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• v.3 no.2
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• pp.177-183
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• 1976

This study was done to find out the effect of hay and silage versus soilage on the rabbit meat prodution for summer period from June 1 to September 28, 1976. Fourty eight rabbits were fed by hay. silage and soilage. respectively. with some amount of concentrate. and the results obtained were as follow. Daily body weight gain of rabbits were slightly higher in soilage feeding group as 16.86g compared with other groups but not significantly different. And the mortality was the highest in soilage feeding group as 16.7% and silage feeding group was 8.3%. but hay feeding group showed the best health condition during the period of experiment. Feed efficiency was slightly better in silage feeding group as 5,61 than other groups but not significantly' different. The dressing percentage was higher in hay feeding group as 49% and lower in soilage feeding group as 46.79%. It was concluded that high quality of rabbit meat could be produced by high quality of hay rather than by silage and soilage for summer period.

• Korean Journal of Agricultural Science
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• v.4 no.2
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• pp.146-156
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• 1977

To obtain some results of the rate of abnormal keton body in urin of dairy animals by age calving rate, milk yield and some feeding and management condition, 104 cows and 107 dairy goats were investigated. And the results obtained were summerized as follows. 1. From about 23.1% of cows and 39.3% goats, abonormal condition of keton body in urine dectated. 2. The rate of abnormal keton bodies possession were lower at the age of 1~4 but higher at the age of 4~11. 3. From the calving animals, the highest rate of keton body were detected but the lowest from first and ninths calving animals. 4. The cows than 20kg milking per day and the goats 3~4kg milking were higher but the cows below 10kg milking and the goods 1~2kg milking were lower. 5. Under bad feeding and management condition, the amount keton bodies in urine of animals were increased much.

• Journal of Microbiology and Biotechnology
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• v.25 no.1
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• pp.98-108
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• 2015

Staphylococcus aureus is an important foodborne pathogen that causes diverse diseases ranging from minor infections to life-threatening conditions in humans and animals. To further understand its pathogenesis, the genome of the strain S. aureus FORC_001 was isolated from a contaminated food. Its genome consists of 2,886,017 bp double-stranded DNA with a GC content of 32.8%. It is predicted to contain 2,728 open reading frames, 57 tRNAs, and 6 rRNA operons, including 1 additional 5S rRNA gene. Comparative phylogenetic tree analysis of 40 complete S. aureus genome sequences using average nucleotide identity (ANI) revealed that strain FORC_001 belonged to Group I. The closest phylogenetic match was S. aureus MRSA252, according to a whole-genome ANI (99.87%), suggesting that they might share a common ancestor. Comparative genome analysis of FORC_001 and MRSA252 revealed two non-homologous regions: Regions I and II. The presence of various antibiotic resistance genes, including the SCCmec cluster in Region I of MRSA252, suggests that this strain might have acquired the SCCmec cluster to adapt to specific environments containing methicillin. Region II of both genomes contains prophage regions but their DNA sequence identity is very low, suggesting that the prophages might differ. This is the first report of the complete genome sequence of S. aureus isolated from a real foodborne outbreak in South Korea. This report would be helpful to extend our understanding about the genome, general characteristics, and virulence factors of S. aureus for further studies of pathogenesis, rapid detection, and epidemiological investigation in foodborne outbreak.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.3
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• pp.201-206
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• 2004

This experiment was performed to evaluate the agronomic characteristics and dry matter yields of the early maturing cultivars(c.v.'Koolgrazer', '9909,' 'Ol-homil' and 'Jlee select') the medium maturing cultivar(c.v. 'Homil 22') and late maturing cultivars(c.v. 'Danko', 'Kior', 'Amilo' and 'Spooner'.) Seeds were sown on October 3, 2002 and October 8, 2003, respectively at the Livestock Experiment Farm of Keimyung College, Seongju, Kyeongbuk Province. Forage rye was harvested on April 22, 2003 and April 23, 2004, respectively. The height of early maturing cultivars were higher than that of late maturing cultivars at the harvest. Fifty percent heading date of the early maturing cultivar was on April 14 and that of late maturing cultivars were on April 24. In the first year, dry matter(DM) yield of 'Jlee select' was significantly the highest in among all cultivars(p < 0.05). DM yield of early maturing cultivars were higher than that of medium and later maturing cultivars. In the second year, DM yield of '9909' was significantly the highest in among all cultivars(p < 0.05). The early maturing cultivar of 'Koolgrazer' and 'Ol-homil' were similar and 'Jlee select' was low, late maturing cultivar of 'Danko', 'Kior', 'Amilo' and 'Spooner' were lower than early maturing cultivar. According to the results obtained from the study, it is suggested that the early maturing cultivar would be recommendable for fall sown rye for double cropping forage production.

• Korean Journal of Agricultural Science
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• v.4 no.1
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• pp.61-65
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• 1977

To select some salt tolerant pasture species for grassland establishment on the west coastal saline land in Korea, this work was carried out from March to October in 1976 with 5 pasture seeds mainly were introduced from Australia. On the soil salt content was 0.4%, all seeds germination were failure, but all species were survived on the 0.2% salt content land. The yield of forage were much better from Kikuyu grass and Pearl millet but poor from other species. The Meadow grass could be used for protect soil erosion by lot of tillers. By the result from this experiment, only Kikuyu grass and Pearl millet were possible to use for forage crops. But to continue this work, it is suggested to introduced more effective species such like Saltwater couch, Saltshore ryegrass, Puccinella, Salt bush, Blue buch, Samphire and so on.

• Korean Journal of Microbiology
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• v.55 no.3
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• pp.313-315
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• 2019

An anaerobic bacterial strain WCF-2 was isolated from cow dung in finding cellulose-degrading bacteria for use as silage additives. Strain WCF-2 showed a higher cellulolytic activity than Cellulosilyticum lentocellum DSM $5427^T$, the closest relative of strain WCF-2 (98.2% of 16S rRNA gene sequence similarity). We sequenced the complete genome of strain WCF-2 and compared it with that of C. lentocellum DSM $5427^T$. The OrthoANI value between the two strains was 97.9% thus strain WCF-2 was identified as C. lentocellum. The genome size of strain WCF-2 was 4,779,774 bp with a G + C content of 34.4%, 4,154 coding genes (CDS), 54 pseudo genes, and 142 RNA genes. Strain WCF-2 harbored seven cellulase genes, five of which showed low similarities with those of C. lentocellum DSM $5427^T$.

• Journal of Microbiology and Biotechnology
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• v.33 no.7
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• pp.909-914
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• 2023

While searching for the bacteria which are responsible for degradation of pesticide in soybean field soil, a novel bacterial strain, designated 5-5^T, was isolated. The cells of the strain were Gram-staining-positive, aerobic and non-motile rods. Growth occurred at 10-42℃ (optimum, 30℃), pH 5.5-9.0 (optimum, pH 7.0-7.5), and 0-2% (w/v) NaCl (optimum, 1%). The predominant fatty acids were C_15:0 anteiso, C_17:0 anteiso, and summed feature 8 (C_18:1 ω7c and/or C_18:1 ω6c). The predominant menaquinone was MK-9 (H₂). Diphosphatidylglycerol, glycolipids, phosphatidylinositol, and phosphatidylglycerol were the major polar lipids. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain 5-5^T is a member of the genus Sinomonas and its closest relative is Sinomonas humi MUSC 117^T, sharing a genetic similarity of 98.4%. The draft genome of strain 5-5^T was 4,727,205 bp long with an N50 contig of 4,464,284 bp. Genomic DNA G+C content of strain 5-5^T was68.0 mol%. The average nucleotide identity (ANI) values between strain 5-5^T and its closest strains S. humi MUSC 117^T and S. susongensis A31^T were 87.0, and 84.3 % respectively. In silico DNA-DNA hybridization values between strain 5-5^T and its closest strains S. humi MUSC 117^T and S. susongensis A31^T were 32.5% and 27.9% respectively. Based on the ANI and in silico DNA-DNA hybridization analyses, the 5-5^T strain was considered as novel species belonging to the genus Sinomonas. On the basis of the results from phenotypic, genotypic and chemotaxonomic analyses, strain 5-5^T represents a novel speciesof the genus Sinomonas, for which the name Sinomonas terrae sp. nov. is proposed. The type strain is 5-5^T (=KCTC 49650^T =NBRC 115790^T).

• Journal of Ginseng Research
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• v.47 no.6
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• pp.726-734
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• 2023

Background: Skeletal muscles play a key role in physical activity and energy metabolism. The loss of skeletal muscle mass can cause problems related to metabolism and physical activity. Studies are being conducted to prevent such diseases by increasing the mass and regeneration capacity of muscles. Ginsenoside Rg5 has been reported to exhibit a broad range of pharmacological activities. However, studies on the effects of Rg5 on muscle differentiation and growth are scarce. Methods: To investigate the effects of Rg5 on myogenesis, C2C12 myoblasts were induced to differentiate with Rg5, followed by immunoblotting, immunostaining, and qRT-PCR for myogenic markers and promyogenic signaling (p38MAPK). Immunoprecipitation confirmed that Rg5 increased the interaction between MyoD and E2A via p38MAPK. To investigate the effects of Rg5 on prevention of muscle mass loss, C2C12 myotubes were treated with dexamethasone to induce muscle atrophy. Immunoblotting, immunostaining, and qRT-PCR were performed for myogenic markers, Akt/mTOR signaling for protein synthesis, and atrophy-related genes (Atrogin-1 and MuRF1). Results: Rg5 promoted C2C12 myoblast differentiation through phosphorylation of p38MAPK and MyoD/E2A heterodimerization. Furthermore, Rg5 stimulated C2C12 myotube hypertrophy via phosphorylation of Akt/mTOR. Phosphorylation of Akt induces FoxO3a phosphorylation, which reduces the expression of Atrogin-1 and MuRF1. Conclusion: This study provides an understanding of how Rg5 promotes myogenesis and hypertrophy and prevents dexamethasone-induced muscle atrophy. The study is the first, to the best of our knowledge, to show that Rg5 promotes muscle regeneration and to suggest that Rg5 can be used for therapeutic intervention of muscle weakness and atrophy, including cancer cachexia.

• Korean Journal of Materials Research
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• v.7 no.2
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• pp.152-156
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• 1997

In this paper, I meritioned ,I study on thc rnechanochernic;~l effec ts of grountl iljckite To investigate the mechanochemical effects of ground tlickite 1,y planet,~r\- mill. a structul-ill i~nalvsis of dickite prepared with different grinding time has been made 11y X-ray diffraction and ilifierenti,~l tl~i.~-ni;~i analysis. 'l'he $SiO_{4}$ hecira was unchanged bl- milling and remained ;is the local ordering unit s t t - ~ c ~ u ~ - c ~ s in the ground dickitc samples I ) v analyzing the radial distribution function. On the other hand, with an increi~se of the grinding time a decrease of coordination number and atomic distance bet\vccn aluminum and oxygen hi1c.r l~een octurreil, and Li~~antitativcl\. coniirrned them This result corresponded to the local c,hange around aliimi~iuni I>V tliftercntii~l t1ii:rm;il ani~lysis. Therefore, the mechanochemical phenomina of ground tiickite werc3 h e to the local c~l~mge around aluminum \IT. the prolonged grinding.