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http://dx.doi.org/10.7845/kjm.2019.9103

Complete genome sequence of Celluosilyticum lentocellum WCF-2 isolated from cow dung  

Heo, Jun (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration)
You, Jaehong (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration)
Park, InCheol (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration)
Han, Byeong-Hak (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration)
Kwon, Soon-Wo (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration)
Ahn, Jae-Hyung (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration)
Publication Information
Korean Journal of Microbiology / v.55, no.3, 2019 , pp. 313-315 More about this Journal
Abstract
An anaerobic bacterial strain WCF-2 was isolated from cow dung in finding cellulose-degrading bacteria for use as silage additives. Strain WCF-2 showed a higher cellulolytic activity than Cellulosilyticum lentocellum DSM $5427^T$, the closest relative of strain WCF-2 (98.2% of 16S rRNA gene sequence similarity). We sequenced the complete genome of strain WCF-2 and compared it with that of C. lentocellum DSM $5427^T$. The OrthoANI value between the two strains was 97.9% thus strain WCF-2 was identified as C. lentocellum. The genome size of strain WCF-2 was 4,779,774 bp with a G + C content of 34.4%, 4,154 coding genes (CDS), 54 pseudo genes, and 142 RNA genes. Strain WCF-2 harbored seven cellulase genes, five of which showed low similarities with those of C. lentocellum DSM $5427^T$.
Keywords
Cellulosilyticum lentocellum; cellulolytic activity; genome sequence;
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