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http://dx.doi.org/10.4014/jmb.1410.10005

Comparative Genomic Analysis of Staphylococcus aureus FORC_001 and S. aureus MRSA252 Reveals the Characteristics of Antibiotic Resistance and Virulence Factors for Human Infection  

Lim, Sooyeon (Department of Agricultural Biotechnology, Seoul National University)
Lee, Dong-Hoon (Department of Food Science and Biotechnology and Institute of Life Science and Resources, Kyung Hee University)
Kwak, Woori (C&K Genomics, Seoul National University Research Park)
Shin, Hakdong (Department of Agricultural Biotechnology, Seoul National University)
Ku, Hye-Jin (Department of Food Science and Biotechnology and Institute of Life Science and Resources, Kyung Hee University)
Lee, Jong-eun (DNA Link, Inc., Asan Institute for Life Sciences)
Lee, Gun Eui (DNA Link, Inc., Asan Institute for Life Sciences)
Kim, Heebal (Department of Agricultural Biotechnology, Seoul National University)
Choi, Sang-Ho (Department of Agricultural Biotechnology, Seoul National University)
Ryu, Sangryeol (Department of Agricultural Biotechnology, Seoul National University)
Lee, Ju-Hoon (Department of Food Science and Biotechnology and Institute of Life Science and Resources, Kyung Hee University)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.1, 2015 , pp. 98-108 More about this Journal
Abstract
Staphylococcus aureus is an important foodborne pathogen that causes diverse diseases ranging from minor infections to life-threatening conditions in humans and animals. To further understand its pathogenesis, the genome of the strain S. aureus FORC_001 was isolated from a contaminated food. Its genome consists of 2,886,017 bp double-stranded DNA with a GC content of 32.8%. It is predicted to contain 2,728 open reading frames, 57 tRNAs, and 6 rRNA operons, including 1 additional 5S rRNA gene. Comparative phylogenetic tree analysis of 40 complete S. aureus genome sequences using average nucleotide identity (ANI) revealed that strain FORC_001 belonged to Group I. The closest phylogenetic match was S. aureus MRSA252, according to a whole-genome ANI (99.87%), suggesting that they might share a common ancestor. Comparative genome analysis of FORC_001 and MRSA252 revealed two non-homologous regions: Regions I and II. The presence of various antibiotic resistance genes, including the SCCmec cluster in Region I of MRSA252, suggests that this strain might have acquired the SCCmec cluster to adapt to specific environments containing methicillin. Region II of both genomes contains prophage regions but their DNA sequence identity is very low, suggesting that the prophages might differ. This is the first report of the complete genome sequence of S. aureus isolated from a real foodborne outbreak in South Korea. This report would be helpful to extend our understanding about the genome, general characteristics, and virulence factors of S. aureus for further studies of pathogenesis, rapid detection, and epidemiological investigation in foodborne outbreak.
Keywords
Staphylococcus aureus; genome; foodborne pathogen; antibiotic resistance; virulence factor;
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