• Asian-Australasian Journal of Animal Sciences
• /
• 제27권2호
• /
• pp.209-216
• /
• 2014

The present study was conducted to estimate the NE values of corn, dried distillers grains with solubles (DDGS) and wheat bran (WB) for laying hens based on an indirect calorimetry method and nitrogen balance measurements. A total of 576 twenty-eight-wk-old Dwarf Pink-shell laying hens were randomly assigned to four groups fed a basal diet (BD) or a combination of BD with 50% corn or 20% DDGS or 20% WB, with four replicates each. After a 7-d adaptation period, each replicate with 36 hens were kept in one of the two respiration chambers to measure the heat production (HP) for 6 days during the feeding period and subsequent 3-d fasting. The equilibrium fasting HP (FHP) provided an estimate of NE requirements for maintenance (NEm). The NE values of test feedstuffs was estimated using the difference method. Results showed that the heat increment that contributed 35.34 to 37.85% of ME intake was not influenced by experimental diets (p>0.05) when expressed as Mcal/kg of DM feed intake. Lighting increased the HP in hens in an fed-state. The FHP decreased over time (p<0.05) with the lowest value determined on the third day of starvation. No significant difference between treatments was found on FHP of d 3 (p>0.05). The estimated AME, AMEn, and NE values were 3.46, 3.44 and 2.25 Mcal/kg DM for corn, 3.11, 2.79, and 1.80 Mcal/kg DM for DDGS, 2.14, 2.10, and 1.14 Mcal/kg DM for WB, respectively. The net availability of AME of corn tended to be numerically higher than DDGS and WB (p = 0.096). In conclusion, compared with corn, the energy values of DDGS and WB were overestimated when expressed on an AME basis.

• Asian-Australasian Journal of Animal Sciences
• /
• 제21권3호
• /
• pp.420-425
• /
• 2008

The storage of rice polishing (RP) with and without addition of antioxidant for sixteen weeks and its effect on rancidity and metabolizable energy values during the summer season was determined. Fifteen Single Comb White Leghorn cockerels of approximately uniform age and weight were procured and kept in metabolic cages under standard feeding and management practices. Five force feeding trials were conducted. In the first trial, fresh RP with 0 weeks of storage (diet 1 and 2) was used followed by four feeding trials with 4 (diet 3, 4), 8 (diet 5, 6), 12 (diet 7, 8), and 16 (diet 9, 10) weeks of storage of RP. The same birds were used in all trails. The birds were fasted for a period of 21 h, followed by force feeding of 20 g of RP with and without antioxidant for all storage periods. The control/fasting group was also maintained to measure endogenous fecal losses. Excreta were collected after 48 h for the determination of AME and TME values of RP. Along with the biological trials, laboratory assay of the RP stored with and without antioxidant was conducted to measure the extent of rancidity in terms of Thiobarbituric acid value (TBA). The TBA values were affected (p<0.05) by storage period and the values increased when the storage period increased from 4 to 16 weeks. However, the TBA values were significantly reduced (p<0.05) when RP was stored after addition of antioxidant when compared with the values obtained from RP stored without antioxidant (diet 3 vs. 4, 5 vs. 6, 7 vs. 8, and 9 vs. 10). The AME MJ/kg and TME MJ/kg values of RP were neither affected by increase in storage period nor addition of antioxidant. The findings of this study revealed that there was no effect of rancidity and storage time on the nutritive value, AME or TME of RP in poultry. However, TBA values were increased with the increase in storage period.

• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2009년도 학술발표회 초록집
• /
• pp.1698-1702
• /
• 2009

효과적인 저수지 수질관리를 위해서는 신뢰도 높은 수리 및 수질모델이 필요하며, 이러한 모델의 성능은 다양한 수문사상에 대하여 적용함으로써 검증할 수 있다. CE-QUAL-W2 모델(이후 W2)은 횡방향 평균 2차원 수리 수질 해석 모델로써 수체의 길이에 비해 폭이 상대적으로 좁고 수심이 깊은 우리나라 대부분의 저수지 지형에 적합한 모델이다. 본 연구의 목적은 기존 연구에서 가뭄년인 2001년과 평수년인 2004년 수문사상에 대하여 보정한 대청호 W2 부영양화모델을 최근 평수년인 2006년과 가뭄년인 2008년을 대상으로 검증하는데 있다. 모델의 검증은 물수지, 수온성층 구조 변화, 부영양화 해석에 중점을 두었으며, 실측자료와 모의결과의 적합성 비교 평가는 결정계수값$(R^2)$, AME(absolute mean error)와 RMSE(root mean square error)를 이용하였다. 저수지 물수지의 적합성을 검증하기 위하여 모의수위와 실측수위를 비교한 결과, $R^2$값이 2006년과 2008년에 각각 0.9945, 0.9972로 나타나 신뢰도가 높은 것으로 확인되었다. 계절별 성층구조 변화 모의 성능을 검증하기 위해 회남수역과 댐 앞 지점에서 수심별 수온의 모의값과 실측값을 비교하였다. 2006년의 경우 모델은 홍수기 동안 안정적으로 수온 성층현상을 모의하였으나, 댐 앞 지점에서 수온 약층이 형성된 구간에서 실측값과 다소 편차를 보였으며, 오차크기는 AME가 $0.561\sim2.088^{\circ}C$, RMSE는 $0.797\sim2.762^{\circ}C$범위였다. 반면, 가뭄년인 2008년에는 전 기간에 걸쳐 모두 안정적으로 저수지 수온 성층현상을 모의하였으며, 오차크기는 AME $0.413\sim1.162^{\circ}C$, RMSE $0.546\sim1.415^{\circ}C$ 범위였다. 조류의 생산성이 높은 표층에서 T-N, T-P 및 Chl-a 농도 모의결과를 장계교, 대정리, 회남대교, 댐 앞, 추동취수탑 및 문의취수탑에서 시계열로 실측값과 비교 검증한 결과, T-N과 T-P는 2006년과 2008년 모두 모든 비교 지점에서 모의값과 실측값의 시계열 변동이 매우 잘 일치하였으며, 홍수기 이외 기간에는 큰 변동 폭을 보이지 않았다. 그러나 기존 연구에서 확인된 바와 같이 7월 이후부터 T-P 모의값이 실측값을 과대 산정하는 경향을 보였는데, 그 원인은 산소 결핍상태에서 저니층에서 용출되는 철(Fe) 또는 망간(Mn)과 같은 이온 성분이 인과 흡착하여 침전되는 기작이 모의과정에 적절히 반영되지 않은 것이 원인으로 판단된다. 조류(Chl-a)농도의 경우, 2006년과 2008년에 모든 지점에서 모델은 조류의 발생과 시계열 변화를 적절히 모의하였으나, 2008년 1월부터 8월까지 댐 앞과 추동 및 문의취수탑에서는 모의값이 실측값을 과대 산정하는 경향을 보였다. 이는 해마다 그리고 계절별로 우점하는 조류 종이 상이한 반면, 모델에서는 이에 대한 매개변수를 적절히 고려하지 못한 것이 원인으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제16권3호
• /
• pp.394-402
• /
• 2003

Individual and combined supplementation of phosphorus-adequate, wheat-based broiler diets with exogenous phytase and xylanase was evaluated in three experiments. The effects of the enzyme combination in lysine-eficient diets containing wheat and sorghum were more pronounced than those of the individual feed enzymes. The inclusion of phytase plus xylanase improved (p<0.05) weight gains (7.3%) and feed efficiency (7.0%) of broilers (7-28 days post-hatch) and apparent metabolisable energy (AME) by 0.76 MJ/kg DM. Phytase plus xylanase increased (p<0.05) the overall, apparent ileal digestibility of amino acids by 4.5% (0.781 to 0.816); this was greater than the responses to either phytase (3.6%; 0.781 to 0.809) or xylanase (0.7%; 0.781 to 0.784). Absolute increases in amino acid digestibility with the combination exceeded the sum of the individual increases generated by phytase and xylanase for alanine, aspartic acid, glutamic acid, glycine, histidine, isoleucine, phenylalanine, threonine, tyrosine and valine. These synergistic responses may have resulted from phytase and xylanase having complementary modes of action for enhancing amino acid digestibilities and/or facilitating substrate access. The two remaining experiments were almost identical except wheat used in Experiment 2 had a higher phytate concentration and a lower estimated AME content than wheat used in Experiment 3. Individually, phytase and xylanase were generally more effective in Experiment 2, which probably reflects the higher dietary substrate levels present. Phytase plus xylanase increased (p<0.05) gains (15.4%) and feed efficiency (7.0%) of broiler chicks from 4-24 days post-hatch in Experiment 2; whereas, in Experiment 3, the combination increased (p<0.05) growth to a lesser extent (5.6%) and had no effect on feed efficiency. This difference in performance responses appeared to be 'rotein driven'as the combination increased (p<0.05) nitrogen retention in Experiment 2 but not in Experiment 3; whereas phytase plus xylanase significantly increased AME in both experiments. In Experiments 2 and 3 the combined inclusion levels of phytase and xylanase were lower that the individual additions, which demonstrates the benefits of simultaneously including phytase and xylanase in wheat-based poultry diets.

• 식물병연구
• /
• 제22권3호
• /
• pp.208-212
• /
• 2016

무안지역의 양파 농가에서 육묘 재배가 시작된 2013년 9월 중 하순경부터 본밭에 정식이 이루어진 11월 하순까지의 온도 및 상대습도 조건이 양파 노균병 발병에 적합한 환경에 해당하였다. 그래서 육묘 단계에서 발생하는 양파 노균병의 방제를 위해 살균제를 이용하여 연구를 수행한 결과, 양파 육묘상에 서로 다른 살균제들이 처리된 각각의 처리구에 비해서 무처리구에서는 51.1%의 이병주율을 보여 살균제 효과를 평가하기에 충분하였다. 다양한 작용기작을 가지고 있고 포자발아 억제 효과가 우수하여 보호살균제로 많이 사용되고 있는 mancozeb을 세 차례 살포한 MAN 처리구의 이병주율이 4.4%로 나타나 방제가가 91.4%로 조사되어 가장 우수한 방제 효과를 보였고, 작용기작이 각각 다른 살균제인 amectotradin+dimethomorph, fluopicolide+propamocarb HCl, dimethomorph+pyraclostrobin을 순서대로 살포한 MIX 처리구는 10.0%의 이병주율을 보여 방제가가 80.4%로 나타났다. 그리고 amectotradin+dimethomorph만을 세 차례 살포한 AME+DIM 처리구는 12.2%의 이병주율을 보여 방제가가 76.1%로 나타나 방제 효과가 다른 두 처리구에 비해 다소 낮게 나타났으나 살균제가 처리된 모든 처리구에서 무처리구에 비해서 통계적으로 유의한 차이를 나타내었다. 따라서 양파 육묘 재배 시기가 노균병발병에 적합한 환경에 해당되므로 살균제 등을 사용하여 육묘 단계부터 양파 노균병 방제에 대한 적극적인 노력이 필요하다고 생각한다.

• Journal of Animal Science and Technology
• /
• 제62권3호
• /
• pp.374-384
• /
• 2020

Two experiments were conducted to determine apparent metabolizable energy (AME), nitrogen-corrected AME (AMEn), and ileal digestible amino acid (AA) content of brown rice (BR) and to investigate the effect of dietary supplementation of BR on laying performance and egg quality of laying hens. In Exp. 1, 72 Hy-line Brown layers (49-week-old) were allocated to two treatments using a completely randomized block design, and each treatment included six cages per treatment and six hens per cage. A semi-purified diet was formulated to include BR as the sole source of AA and energy and an N-free diet was used to determine basal endogenous loss of AA. The hens were fed a commercial layer diet for adaptation to the experimental environment and diet for 7 days from d 0, and then fed experimental diets for 5 days from d 7. Excreta were collected from d 10 to 11 and ileal digesta were collected on d 12. On a dry matter (DM) basis, the AME and AMEn of BR was determined at 3,773 and 3,729 kcal/kg, respectively. The apparent ileal digestibility (AID) of BR ranged from 32.7% for Thr to 73.7% for Arg. The range of the standardized ileal digestibility (SID) value was between 79.4% for Met and 96.6% for Lys. In Exp. 2, 252 Hy-line Brown layers (44-week-old) were divided into four groups, comprising seven replicates of nine birds each and assigned to four experimental diets containing 0 (Control), 5%, 10%, or 15% BR for 5 weeks. The BR-containing diets were formulated to be equal in the content of AMEn and digestible AA to those of the diet without BR. No significant differences were observed in laying performances. Egg quality and blood profiles were not linearly or quadratically affected by dietary treatments. These results suggest that up to 15% BR can be included into layer feed without any adverse effects on laying performance and egg quality, if its energy and digestible AA values are well evaluated.

• Asian-Australasian Journal of Animal Sciences
• /
• 제32권4호
• /
• pp.564-573
• /
• 2019

Objective: Evaluate the effects of i) dehulling of lupine seed on chemical composition and apparent metabolizable energy (AME) and ii) soybean meal substitution by dehulled lupine seed in broiler diets with enzymes on productive performance, size of digestive organs and welfare-related variables. Methods: Experiment 1, chemical composition and AME were determined in whole and dehulled lupine seed. Experiment 2, two hundred eighty-eight one-day-old male Ross 308 broilers were used. The experimental diets were maize-soybean meal (MS), MS with enzymes (MSE) and maize-dehulled lupine seed with enzymes (MLE). Diets were assigned to the experimental units under a completely randomized design (eight replicates per diet). The body weight (BW) gain, feed intake, feed conversion, digestive organ weights, gait score, latency to lie down and valgus/varus angulation were evaluated. Results: The dehulling process increased protein (25.0% to 31.1%), AME (5.9 to 8.8 MJ/kg) and amino acid contents. The BW gain of broilers fed the MLE diet was similar (p>0.05) to that of those fed the MS diet, but lower than that of those fed the MSE diet. Feed intake of broilers fed the MLE diet was higher (p<0.05) than that of those fed the MS diet and similar (p>0.05) to those fed the MSE diet. Feed conversion of broilers fed the MLE diet was 8.0% and 8.7% higher (p<0.05) than that of those fed the MS and MSE diets, respectively. Broilers fed the MLE diet had the highest (p<0.05) relative proventriculus and gizzard weights, but had poor welfare-related variables. Conclusion: It is possible to substitute soybean meal by dehulled lupine seed with enzymes in broiler diets, obtaining similar BW gains in broilers fed the MLE and MS diets; however, a higher feed intake is required. Additionally, the MLE diet reduced welfare-related variables.

• Journal of the Korean Data and Information Science Society
• /
• 제18권2호
• /
• pp.573-584
• /
• 2007

We define a skew-symmetric Laplace distribution by a symmetric Laplace distribution and evaluate its coefficient of skewness. And we derive an approximate maximum likelihood estimator(AME) and a moment estimator(MME) of a skewed parameter in a skew-symmetric Laplace distribution, and hence compare simulated mean squared errors of those estimators. We compare asymptotic mean squared errors of two defined estimators of reliability in two independent skew-symmetric distributions.

• 한국환경보건학회지
• /
• 제15권1호
• /
• pp.1-9
• /
• 1989

환경인자가 인체의 발암에 크게 기여하며, 대기오염도 흡연인자와 더불어 폐암발생의 주요인자로 주목되고 있다. 발암성 시험은 mouse, rat 등의 소동물을 이용하는 in vivo 시험법이 원칙이나, 시간과 경비가 많이 소요되므로, in vitro 생물시험법이 현재 발암물질의 screening 수법으로 많이 이용되고 있다. 특히 B.N.Ames에 의해 개발된 Salmonella균 변이주를 이용하는 방법은, 간편성, 신속성에 뛰어나고, 정성적 또는 정량적으로 발암성과 매우 높은 상관관계를 보이고 있어 최근 10여년간에 걸쳐, 수많은 화학물질 또는 대기오염물질과 같은 복합오염물질의 변이원성 및 발암성의 screening test로서 널리 응용되어 왔다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제22권1호
• /
• pp.107-112
• /
• 2009

This study was conducted to investigate the effect of feed particle size and feed form on growth performance, nutrient metabolizability and intestinal morphology in broiler chickens. This experiment was a 2${\times}$2 factorial arrangement including two feed particle sizes (fine and coarse) and two feed forms (mash and pellet). A total of two hundred and eighty eight day-old male Arbor Acre broilers were used in this six week experiment. Birds were randomly allotted to four dietary treatments with six replicates per treatment and twelve birds per replicate. The results showed that pelleting diets resulted in greater ADG (p<0.01), greater ADFI (p<0.01) and lower feed to gain ratio (F/G) (p<0.05) during starter, grower and overall period. Also, pelleting improved both apparent metabolizable energy (AME) (p<0.01) and the apparent metabolizability of crude protein (p<0.05) and organic matter (p<0.05) regardless of the phase. Reduction of feed particle size enhanced AME (p<0.05) during d 19 to 21. Increased villus height (p<0.05) and crypt depth ratio (p< 0.05) within duodenum, jejunum, and ileum were observed in birds fed the pellet diet compared with those given the mash diet. In conclusion, results indicated that feed pellets might enhance performance by improving nutrient metabolizability and digestive tract development.