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SNP Marker Development for Purity Test of Oriental Melon and Melon (멜론 및 참외 순도 검정을 위한 SNP 마커 개발 및 F1 종자 순도 검정)

  • An, Song-Ji;Kwon, Jin-Kyung;Yang, Hee-Bum;Choi, Hye-Jeong;Jeong, Hee-Jin;Kim, Yong-Jae;Choi, Gyung-Ja;Kang, Byoung-Cheorl
    • Korean Journal of Breeding Science
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    • v.42 no.4
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    • pp.397-406
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    • 2010
  • Field screening method has been commonly used for purity test of $F_1$ hybrid seeds in melon and oriental melon. However, as this method takes a lot of time and cost, molecular marker-based purity test is necessary. To develop molecular markers for purity test, thirty pairs of SNP (single nucleotide polymorphism) primers were obtained from melon EST sequences, and 10 polymorphic markers showing HRM (high resolution melting) polymorphisms between parents of two melon cultivars and one oriental melon cultivar were selected. Blind tests were performed to validate usefulness of the selected markers for purity test. Blind test results showed that HRM genotypes were matched with the expected identity of individual sample, $F_1$ hybrid, male or female parents. Three HRM-based SNP markers were converted to CAPS markers for general use which is favor to breeders. We expect that SNP markers developed in this study will be useful for purity test of $F_1$ hybrid seeds in melon and oriental melon.

Development and Complementation of Evaluation Area and Content Elements in Electrical, Electronics and Communications Subject (중등교사 임용후보자선정경쟁시험 표시과목인 전기·전자·통신의 평가영역 및 내용요소 개발·보완 연구)

  • Song, Youngjik;Kang, Yoonkook;Cho, Hanwook;Gim, Seongdeuk;Lim, Seunggak;Lee, Hyuksoo
    • 대한공업교육학회지
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    • v.44 no.1
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    • pp.52-71
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    • 2019
  • The quality of school education is a key element for national education development. An important factor that determines the quality of school education is qualities of teachers who are in responsible for school education in the field. Therefore, it is necessary to hire competent teachers in the teacher appointment exam for the secondary school. This necessity is evident especially for vocational high schools and Meister high schools with the introduction of 2015-revised curriculum based on NCS that separates each three subjects, "Electrical, Electronics Communication" resulting in the change of question mechanism, which requires new designing of assessment and content area. So, this study analyzes curriculum in college of education for "Electrical", "Electronics", "Communication", 2015-revised curriculum based on NCS and the development of standards for teacher qualifications and assessment area and evaluation of teaching ability in the subjects of the teacher appointment exam, "Electrical, Electronics Communication" Engineering" in 2009. The assessment area and content elements of "Electrical", "Electronics", "Communication are extracted from the analyzed results and they are verified by experts' consultation and presented as follows; First, the assessment area and content elements of the "Electrical" subject were designed to evaluate the NCS - based 2015 revised curriculum by presenting the NCS learning module to the evaluation area and content element in the basic subject "Electrical and Electronics Practice". Second, the section of "Electronics" presented the assessment area and content elements applying the Electronic Circuit, basic subject of the NCS and it also added "Electromagnetics", which is the basic part of Electronics in the Application of Electromagnetic waves that could be applied to the assessment. Third, the assessment area and content elements of "Communication" consist of the communication-related practice that is based on "Electrical" and "Electronic", considering the characteristics of "Communication Engineering". In particular, "Electrical and Electronics practice" which adds network construction practice and communication-related practice makes it to be able to evaluate the communication-related practical education.

Comparison of Quality and Bioactive Compounds in Chicken Thigh Meat from Conventional and Animal Welfare Farm in Korea (국내 일반 및 동물복지 육계 다리육의 냉장 저장 기간 중 품질과 생리활성기능 성분 비교)

  • Kim, Hee-Jin;Kim, Hye-Jin;Jeon, Jin-Ju;Oh, Sang-Jip;Nam, Ki-Chang;Shim, Kwan-Seob;Jung, Jong-Hyun;Kim, Kyong Su;Choi, Yang-Il;Kim, Sang-Ho;Jang, Aera
    • Korean Journal of Poultry Science
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    • v.45 no.4
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    • pp.261-272
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    • 2018
  • This study was conducted to evaluate the difference in the quality of chicken thigh meat from conventional and animal welfare farms during refrigeration storage over 9 days. Chicken thigh meat from conventional (CTC, n = 30) and animal welfare farms (CTW, n = 30) was tested. The pH value was significantly lower in CTW (6.28) than in CTC (6.37) on day 1; however, no significant differences were found on subsequent days. The yellowness of CTW was higher than that of CTC on day 1, but CTW showed lower yellowness than did CTC on day 7 and 9. The cooking loss, water holding capacity, lightness, redness, and coliform levels of CTC did not show any significant difference when compared with CTW during storage. The shear force of CTW was significantly higher than that of CTC on day 1, 3, 7, and 9. Total microorganism and coliform in CTC and CTW increased with increasing storage days. On day 7 and 9, the total microorganism level of CTW was lower than that of CTC. The thiobarbituric acid value of CTW was lower than that of CTC on day 9. The volatile basic nitrogen (VBN) of CTW was lower than that of CTC during storage. Anserine content and 1,1-diphenyl-2-picryl-hydrazyl(DPPH) scavenging activity of CTW was significantly higher than CTC on day 1. These results suggest that CTW stayed fresher for longer than did CTC because of low total microorganism level on day 7 and 9, and VBN during refrigerated storage.

Experiments on Flow Characteristics of Asphalt Seal Composite Waterproofing Method for Underground Concrete Structure Exterior Wall Waterproofing (지하 콘크리트 구조물 외벽 방수용 아스팔트 씰재 복합방수 공법의 흘러내림 특성에 관한 실험)

  • Ko, Sang-Ung;Kim, Kyoung-Hoon;Kim, Young-Sam;Shin, Hong-Chul;Kim, Jin-Man
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.6 no.4
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    • pp.297-303
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    • 2018
  • With the changing trend of the building construction to high rising and large scaling, the underground structure has been increased, and its usage also increased and variety. Hence, to protect the underground structure against underground water, various water proofing methods has been developed. Among the many water proofing methods, the combined water proofing method using both asphalt seal and sheet has been widely used to secure the sufficient performance and decrease the construction failure. However, during the summer period of extremely high temperature conditions, the asphalt sealing materials were separated and leaked into the structure. Therefore, the aim of the research is to provide the quality standard of asphalt sealing material based on the various temperature changes depending on seasons. According to the experimental results, the temperature of the sealing materials applied on the slab was increased up to $54^{\circ}C$ which was $3^{\circ}C$ higher than the structure temperature of $51^{\circ}C$. Based on the melting test for asphalt sealing materials applied on the outside wall of the structure, in the case of water-dispersing typed materials showed significant melting down due to its slow evaporation and low viscosity. Furthermore, from the accelerated test conducted indoor conditions, a solvent-type and water-dispersing typed materials showed significant melting down due to their low viscosity and melting point in ambient conditions. Based on these results, viscosity and melting point are found as the important factors on asphalt sealing materials' quality, and it is necessary to designate the quantitative level of the viscosity and melting point for quality control.

The Issues of Workers' Solidarity and Labor Collectivism in terms of the American Two-Tier Wage Systems (미국 이중임금제를 통해 본 노조 연대와 집단이기주의의 문제)

  • Lee, Jeonghyun
    • International Area Studies Review
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    • v.22 no.3
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    • pp.221-251
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    • 2018
  • Two-tier wage systems mean the dual wage systems that the new wage system require the new future employees to get much lower wage level, compared to the level of wages for existing employees under the existing wage system. While it allows employers to benefit from low-cost advantages, the two-tier wage systems is definitely a type of wage discrimination by the collusion between management and trade unions in that it forces the new future employee to accept the low wages. Also it reflects extreme collectivism which old union members try to keep having their jobs and wages at the sacrifice of future members' wages. The two-tier wage systems had been introduced by airplane industry in 1980s and introduced again in Big Three auto companies in 2007. The purpose of this paper is to examine the history, contents, and details of two-tier wage system in the United States and to think of the possibility of recurrence of the systems in the context of Korean auto industry in the near future. The tentative findings from this paper implies that the two-tier wage systems will likely happen in Korean auto industry because the collusion between trade unions and management are found easily and the degree of extreme collectivism favored by the old permanent union members is seriously high. It is time for trade unions to go back to their original ideals and purposes and to revitalize solidarity among workers.

The Character of Bogamni group and trends in the surrounding area seen through the stone chamber (석실로 본 나주 복암리 세력과 주변 지역의 동향)

  • Kim, Nak jung
    • Korean Journal of Heritage: History & Science
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    • v.49 no.1
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    • pp.44-67
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    • 2016
  • This article studied the character of Bogamri group and trends in the surrounding area through recently excavated stone chambers. The first type of stone chamber constructed at Bogamni cemetry was Northen Kyushu style stone chamber tomb with corridor. However, According to recent survey Wa style vertical stone lined tomb had constructed earlier than these stone chamber along the southwest cost. It is likely that the background of these Wa style stone tomb was the beginning of formal diplomatic relations between Baekje and Wa. At first, grave goods as well as burial had been Wa style, but gradually local burial element and mortuary practices added. And large tomb had beginning to appear. The representative tombs are Yeongam Okyari Jangdong tomb and Naju Gaheugni Shinhueng tomb. They were built in the middle of the fifth century. It is most likely that the location was selected with regard to the use or securing of inland waterway. But the next step, Yeongsangang river style stone chamber with corridor had constructed at Jeongchon tomb. That tomb is very close to Bogamni tombs and structure and grave goods are very similar also. So these two groups were considered to be the same community. Hereby, It is believed that the inland waterway using power had been turned over to Bogamni group. Bogamni group had constantly constructed stone chamber until seventh century. This represent that Bogamni group had thrived as a leading force in the Yeongsangang river basin. After under the direct control of Baekje, Bogamni group had continued the close relationship with Wa. For this reason, Bogamni group had influenced southwest cost as well as Yeongsangang river basin. It can be estimated by the Bogamni style stone chamber founded at islands of Shinan.

The Phenological Responses of Leaf of Deciduous Woody Species to Base Temperature Maintenance (수목 최저 생육온도 이상으로 유지된 조건에서의 한반도 주요 수목 잎의 식물계절학적 반응)

  • Hong, Yongsik;Lee, Seungyeon;Lee, Sooin;Lee, Eungpill;Kim, Euijoo;Park, Jaehoon;Jeong, Heonmo;You, Younghan
    • Korean Journal of Ecology and Environment
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    • v.51 no.4
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    • pp.259-267
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    • 2018
  • This study was conducted to confirm phenological response of main deciduous woody species in Korea according to elevated temperature. Based on seeds collected from the same place, 39 woody species were cultivated in field (control) and in greenhouse (treatment) that was maintained above base temperature ($4.8^{\circ}C$). And then, we observed phenotype change of leaves focused on phenological response and explained relation with their current distribution area. As a result, initiation period of leaves unfolding was 1st~3rd, May in control. It was 13, December~7, January in treatment. Period of leaves yellowing was 11~26, October in control. It was 30, October~13, November in treatment. Consequently, initiation period of leaves unfolding was faster by 119~140 days and period of leaves yellowing was slower by 3~32 days since elevated temperature. Period of leaves growth increased in treatment by 148 days than control. Quercus mongolica and Quercus serrata that cultivated in treatment was changed as evergreen trees which have grown up continuely during one year. Also, initiation period of leaves unfolding of Sorbus alnifolia in treatment was faster than in control. However, difference of the period between control and treatment was smallest. Because period of yellowing leaves was moved ahead. Phenological response of leaves according to elevated temperature had no relation of type of their current distribution area. This is the result of acclimation due to elevated temperature during the winter and suggests that the phenotype of leaves of 39 deciduous woody species is more sensitive to the current growth condition than to the past growth condition.

Monitoring Soil Characteristics and Growth of Pinus densiflora Five Years after Restoration in the Baekdudaegan Ridge (백두대간 마루금 복원사업지에서의 5년 경과 후 토양특성 및 소나무 생장 모니터링)

  • Han, Seung Hyun;Kim, Jung Hwan;Kang, Won Seok;Hwang, Jae Hong;Park, Ki Hyung;Kim, Chan-Beom
    • Korean Journal of Environment and Ecology
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    • v.33 no.4
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    • pp.453-461
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    • 2019
  • This study was conducted to monitor the soil characteristics and growth of Pinus densiflora and to determine the effect of soil characteristics on growth rate five years after an ecological restoration project in Baekdudaegan ridge including Ihwaryeong, Yuksimnyeong, and Beoljae sites. The ecological restoration project was executed with the forest of P. densiflora in 2012-2013. In April 2018, we collected soil samples from each site and measured the height and the diameter at breach height (DBH) of P. densiflora. Although there was no significant change of soil pH compared to the early stage of restoration (one year after the project), it was high in Ihwaryeong, and Beoljae with values of 7.7 and 6.4, respectively. Also, the organic matter decreased by 70-80%, and the available phosphorus (P) was unchanged in three restoration sites. The decreased organic matter can be attributed to restriction of inflow and thus decomposition of litter in the early stage after the restoration. The tree height growth rate ($m\;yr^{-1}$) of P. densiflora in Yuksimnyeong was the highest at 1.02, followed by Beolja at 0.75 and Ihwaryeong at 0.17. The height growth rate showed negative relationships with soil pH and cations, including Na and Ca concentrations and a positive relationship with available phosphate. The low growth rate in the Ihwaryeong site, in particular, might result from the poor nutrient availability due to high soil pH and the decrease in water absorption into the root due to high Na and Ca concentrations. The substantial reduction of organic matter after five years indicates that the need for soil improvement using chemical fertilizer and biochar.

Characteristics of Soil Disturbance Caused by Passages of Harvester and Forwarder in Cut-to-Length Harvesting Operations (단목생산작업에 있어서 하베스터와 포워더의 임내주행에 따른 토양교란 특성)

  • Han, Sang-Kyun;Lee, Kyeong-Cheol;Oh, Jae-Heun;Mun, Ho-Seong;Lee, Sang-Tae;Choi, Yun-Sung;Choi, Byoung-Koo
    • Journal of Korean Society of Forest Science
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    • v.108 no.1
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    • pp.67-76
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    • 2019
  • With an increasing demand of timber production, the use of heavy machinery in forest management has significantly increased, causing the changes of soil physical properties and the decline of long-term site productivity. This study was conducted to evaluate the effects of logging slash (non-slash, slash $7.3kg/m^2$, and slash $11.5kg/m^2$) and machine passes(harvester 1 pass and forwarder 1 to 10 passes) on soil physical properties at 10 cm, 20 cm and 30 cm soil depths in harvester and forwarder operations and also to estimate the degree of soil surface disturbance. The results indicated that soil bulk density in the non-slash treatment site increased 10 %~29 % (25~139 % in soil penetration resistance) at all soil depths, compared with the slash treatment site(slash $11.5kg/m^2$). Therefore, the creation of a slash mat could be an effective way to minimize the changes of soil physical properties. In addition, 92 % of total soil compaction in slash treatment site was created within harvester 1 pass and forwarder 5 passes. In non-slash treatment site, 84 % of total soil compaction was created within first harvester and forwarder passes. The results showed that slash treatment was effective to reduce soil compaction caused by machine passes and also it is necessary to create designed forwarding trails for minimizing soil compaction area at timber harvesting sites.

Characteristic study and optimization of culture conditions for Bacillus amyloliquefaciens SRCM 100731 as probiotic resource for companion animal (Bacillus amyloliquefaciens SRCM 100731의 반려 동물용 프로바이오틱스 소재로서의 특성 규명 및 배양 조건 최적화)

  • Ryu, Myeong Seon;Yang, Hee-Jong;Jeong, Su-Ji;Seo, Ji Won;Ha, Gwangsu;Jeong, Seong-Yeop;Jeong, Do-Youn
    • Korean Journal of Microbiology
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    • v.54 no.4
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    • pp.384-397
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    • 2018
  • The aim of this study is to screen the strains of Bacillus spp. possessing safety, probiotic activity, and so on, which can be utilized as probiotic resource for using the feed and supplement food of companion animal. About 300 isolates were isolated from traditional Korean sauces, four isolates that did not have or produce the six kinds of B. cereus type vomiting and diarrhea toxin genes, ${\beta}$-hemolytic, and three kinds of carcinogenic enzymes were selected. Antibiotic gene retention, cell surface hydrophobicity, antibiotic sensitivity, and glucose utilization were analyzed for four isolates, and finally SRCM 100731 was selected. SRCM 100731 was named as Bacillus amyloliquefaciens SRCM 100731 16S rRNA sequencing analysis, and carried out optimization of cell growth for industrial applications such as pet food and feed. The effects of 14 different components on cell growth were investigated and three significant positive factors, molasses, sodium chloride, and potassium chloride were selected as the main factors based on a Plackett-Burman design. In order to find out optimal concentration on each constituent, we carried out central composite design. The predicted optimized concentrations were 7% molasses, 1.1% sodium chloride, 0.5% potassium chloride. Finally, an overall about 7-fold increase in dry cell weight yield ($12.6625{\pm}0.0658g/L$) was achieved using the optimized medium compared with the non-optimized medium ($1.8273{\pm}0.0214g/L$). This research is expected to be highly utilized in the growing pet industry by establishing optimal cultivation conditions for industrial application as well as screening Bacillus amyloliquefaciens SRCM 100731 as probiotic resource for companion animal.