Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

SNP Marker Development for Purity Test of Oriental Melon and Melon

멜론 및 참외 순도 검정을 위한 SNP 마커 개발 및 F1 종자 순도 검정

  • An, Song-Ji (Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Kwon, Jin-Kyung (Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Yang, Hee-Bum (Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Choi, Hye-Jeong (Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Jeong, Hee-Jin (Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Yong-Jae (NH Seed Research & Development Center) ;
  • Choi, Gyung-Ja (Korea Research Institute of Chemical Technology) ;
  • Kang, Byoung-Cheorl (Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University)
  • 안송지 (서울대학교 농업생명과학대학 식물생산과학부) ;
  • 권진경 (서울대학교 농업생명과학대학 식물생산과학부) ;
  • 양희범 (서울대학교 농업생명과학대학 식물생산과학부) ;
  • 최혜정 (서울대학교 농업생명과학대학 식물생산과학부) ;
  • 정희진 (서울대학교 농업생명과학대학 식물생산과학부) ;
  • 김용재 (농협NH 종묘센터) ;
  • 최경자 (한국화학연구원) ;
  • 강병철 (서울대학교 농업생명과학대학 식물생산과학부)
  • Received : 2010.09.13
  • Published : 2010.09.30
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Abstract

Field screening method has been commonly used for purity test of $F_1$ hybrid seeds in melon and oriental melon. However, as this method takes a lot of time and cost, molecular marker-based purity test is necessary. To develop molecular markers for purity test, thirty pairs of SNP (single nucleotide polymorphism) primers were obtained from melon EST sequences, and 10 polymorphic markers showing HRM (high resolution melting) polymorphisms between parents of two melon cultivars and one oriental melon cultivar were selected. Blind tests were performed to validate usefulness of the selected markers for purity test. Blind test results showed that HRM genotypes were matched with the expected identity of individual sample, $F_1$ hybrid, male or female parents. Three HRM-based SNP markers were converted to CAPS markers for general use which is favor to breeders. We expect that SNP markers developed in this study will be useful for purity test of $F_1$ hybrid seeds in melon and oriental melon.

멜론과 참외의 국내 소비 시장이 확대됨에 따라 다양한 $F_1$ 품종이 개발되고 있다. 멜론과 참외의 $F_1$ 품종의 순도를 검정하기 위해 포장재배 등의 순도검정법이 이용되고 있으나 시간과 노력이 매우 많이 소요되기 때문에 분자마커를 이용한 순도검정법의 개발이 필요하다. 본 연구에서는 멜론의 EST 염기정보로부터 30개의 SNP 프라이머 조합을 고안하여 멜론과 참외의 순도 검정을 위한 HRM분석방법을 개발하였다. 멜론 두 품종과 참외 한 품종의 양친 사이에 HRM 해리곡선의 다형성을 보이는 10개의 마커를 선발하였으며 순도검정 마커를 선발하기 위해 blind test를 실시하였다. Blind test와 HRM 유전형 분석 결과가 일치하였으며 MEL SNP 2번과 12 마커를 이용하여 '레드 퀸'과 '얼쓰 VIP'의 $F_1$ 501개 개체에 대해 순도검정을 실시하였다. HRM분석한 결과 모두 이형집합체로 나타나 100%의 순도를 보였다. 또한 HRM 방법을 이용하여 개발한 SNP 마커를 CAPS 마커로 전환하였다. CAPS 마커는 HRM 분석 마커와 비교하여 볼 때 멜론과 참외의 순도검정용 마커로 더 유용하게 활용될 수 있을 것으로 기대된다.

Keywords

Acknowledgement

Supported by : 농림수산식품부

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