• 철학연구
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• 제126권
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• pp.191-212
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• 2013

이 논문은 기존 욕망의 경제학이 남근 질서에 대한 예속성에서 벗어나있지 못함으로써 리비도 흐름들의 절단과 국소화 작업에 치우쳐 왔음을 비판하며 시작한다. 라캉의 욕망 개념이 결핍과 거세에 한정되어왔기에 리비도 흐름들의 산발성과 표류, 탈주의 가능성과 더불어, 잉여와 과잉이라는 초과성 개념을 제대로 담아내지 못하고 있다. 이러한 거세의 법에 포박된 욕망 개념을 넘어서는 생성과 생산으로서의 욕망 개념인 들뢰즈와 가타리의 논의에서 보다 나아가 homo surplus 잉여적 인간 개념을 창출해 보려 하였다. 미리 주어진 규준틀 자체를 넘어서 버리는 과잉성과 비 측량성으로서의 잉여 개념이 어떻게 남근 질서로부터의 분리와 해체를 가능케 하는지에 주목한다. 그리고 기관 쾌락이라는 제 2세대 재생산을 위한 국소적이며 합목적적, 기능주의적 쾌락 개념의 한계를 날카로이 드러내며, 쥬이상스(jouissance)라는 비남근적 향유가 감각 다발의 재배치라는 급진적 성적 실천으로 이어질 수 있는가를 면밀히 분석해 나갈 것이다.

• ALGAE
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• 제27권4호
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• pp.235-247
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• 2012

Regardless of continuous researches, recent researches on the genus Eucampia (Bacillariophyceae) have focused mainly on E. zodiacus f. zodiacus In the present study, species of the genus Eucampia have been studied based on their morphological characteristics. Eucampia species were collected at 24 sites from July 2008 to June 2011 in Korean coastal waters. Species were mainly identified based on the shape of valve, ocellus, and aperture, along with the length and shape of the bipolar elevations. As a result, five Eucampia species were identified: Eucampia cornuta, E. groenlandica, E. zodiacus f. zodiacus, E. zodiacus f. cylindrocornis, and E. zodiacus var. cornigera. E. cornuta and E. groenlandica have long pervalvar axis length, but the others display short or moderate length. Ocellus shape of E. cornuta, E. groenlandica and E. zodiacus f. cylindrocornis are linear ribs, whereas E. zodiacus f. zodiacus and E. zodiacus var. cornigera have radial ribs with central area. E. cornuta and E. zodiacus f. cylindrocornis have long and narrow cylindrical elevations. E. groenlandica and E. zodiacus f. zodiacus have short and broad elevations with blunt tips. E. zodiacus var. cornigera has long and broad conical elevations. In terms of aperture shape, E. cornuta has large elliptical form, E. groenlandica has almost circular to rounded rectangular form, E. zodiacus f. zodiacus has narrow and elliptical rounded rectangular to a narrow lanceolate form, E. zodiacus f. cylindrocornis has almost rectangular form, and E. zodiacus var. cornigera has rounded rhombic form. On the basis of elevations in broad girdle view, 5 Eucampia taxa could be divided into 3 types: 'narrow H type', E. cornuta and E. groenlandica; 'regular H type', E. zodiacus f. cylindrocornis and E. zodiacus var. cornigera (partial); 'wide H type', E. zodiacus f. zodiacus (almost).

• 디지털융복합연구
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• 제9권4호
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• pp.267-279
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• 2011

인터넷의 발전에 힘입어 다양한 형태의 웹서비스가 출현하고 있다. 특히, 대학에서 e-러닝 서버스는 시간적, 공간적 제약의 한계를 극복해 주고, 여러 가지 편리성과 유용성을 제공해 학생들에게 큰 인기를 얻고 있다. e-러닝 확산에 힘입어 e-러닝 수용 연구는 매우 활발하게 이루어졌다. 많은 연구들은 기술수용모델 연구를 응용해 e-러닝 확산방안을 제시하고 있다. 이러한 연구들의 e-러닝의 이용 용이성과 유용성에 중점을 맞추고 있다. 그렇기 때문에 e-러닝의 핵심이 되는 교육 컨텐츠 측면에서의 e-러닝 활성화를 위한 설명이 미약한 측면이 존재한다. 이에 본 연구에서는 e-러닝 수용에 있어 e-러닝 컨텐츠 품질과 e-러닝 신뢰 측면에서의 접근을 통해 e-러닝 수용 확산 방안을 제시하였다. 본 연구 결과는 대학 당국에 e-러닝 수용 확산에 있어 컨텐츠 측면에서의 전략 수립에 유용한 시사점을 제공해 줄 것이다.

• 한국정보통신학회논문지
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• 제15권10호
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• pp.2209-2216
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• 2011

본 논문에서는 Magnachip $0.18{\mu}m$ 공정을 이용하여 PMIC용 32bit eFuse OTP IP를 설계하였다. eFuse 링크 아래에 N-Well을 두어 프로그램시 eFuse 링크와 p-기판의 VSS가 단락되는 문제점을 해결하였다. 그리고 디코딩된 WERP (WL Enable for Read or Program) 신호가 eFuse OTP 메모리로 바로 입력되는 경우 듀얼 포트 eFuse OTP 메모리 셀의 RWL (Read Word-Line)과 WWL (Write Word-Line)을 선택적으로 활성화해 주는 WL 구동회로를 제안하였다. 또한 BL 프리차징 회로에서 delay chain을 제거하여 제어회로의 레이아웃 면적을 줄였다. 메모리 테스트 장비를 이용하여 제작된 94개의 샘플 die를 측정한 결과 5.5V의 프로그램 전압에서 100%의 수율을 얻었다.

• Journal of Microbiology and Biotechnology
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• 제33권7호
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• pp.964-972
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• 2023

Bacteriophage endolysins are peptidoglycan hydrolases composed of cell binding domain (CBD) and an enzymatically active domain. A phage endolysin CBD can be used for detecting bacteria owing to its high specificity and sensitivity toward the bacterial cell wall. We aimed to develop a method for detection of Enterococcus faecalis using an endolysin CBD. The gene encoding the CBD of ECP3 phage endolysin was cloned into the Escherichia coli expression vector pET21a. A recombinant protein with a C-terminal 6-His-tag (CBD) was expressed and purified using a His-trap column. CBD was adsorbed onto epoxy magnetic beads (eMBs). The bacterial species specificity and sensitivity of bacterial binding to CBD-eMB complexes were determined using the bacterial colony counting from the magnetic separations after the binding reaction between bacteria and CBD-eMB complexes. E. faecalis could bind to CBD-eMB complexes, but other bacteria (such as Enterococcus faecium, Staphylococcus aureus, Escherichia coli, Acinetobacter baumannii, Streptococcus mutans, and Porphyromonas gingivalis) could not. E. faecalis cells were fixed onto CBD-eMB complexes within 1 h, and >78% of viable E. faecalis cells were recovered. The E. faecalis recovery ratio was not affected by the other bacterial species. The detection limit of the CBD-eMB complex for E. faecalis was >17 CFU/ml. We developed a simple method for the specific detection of E. faecalis using bacteriophage endolysin CBD and MBs. This is the first study to determine that the C-terminal region of ECP3 phage endolysin is a highly specific binding site for E. faecalis among other bacterial species.

• 한국산학기술학회논문지
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• 제11권9호
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• pp.3571-3577
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• 2010

본 논문에서는 예상 위치좌표 탐색기법과 이를 균등거리비율 개념인 AEDR(Algorithm for localization using the concept of Equivalent Distance Rate)에 적용한 위치인식 보정 알고리즘인 E&E(Equivalent distance rate & Estimated location coordinator exploration)를 제안 한 후 실험을 통해 성능을 분석하였다. 실험결과, E&E는 4개의 시나리오에서 SDS-TWR의 위치인식 보정성능을 0.60m에서 0.34m로 0.26m 만큼 향상시켰고, E&E는 설정된 시나리오에 따라 기존에 제안된 AEDR과 비교한 결과 위치인식 보정 성능비율이 최대 15% 만큼 우수함을 확인하였다. 또한 E&E의 전체 성능실험 중 99%에서 위치인식 오차가 1m 이내로 측정되었는데 이 정도의 위치인식 보정 성능이라면 제안하는 알고리즘인 E&E는 다양한 위치인식 응용분야에 충분히 적용 가능 할 것으로 판단된다.

• 한국동물생명공학회지
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• 제35권2호
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• pp.155-162
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• 2020

Equine follicle stimulating hormone receptor (eFSHR) has a large extracellular domain and an intracellular domain containing approximately 10 phosphorylation sites within the G protein-coupled receptor. This study was conducted to analyze the function of phosphorylation sties at the eFSHR C-terminal region. We constructed a mutant of eFSHR, in which the C-terminal cytoplasmic tail was truncated at residue 641 (eFSHR-t641). This removed 10 potential phosphorylation sites from the C-terminal region of the intracellular loop. The eFSHR-wild type (eFSHR-wt) and eFSHR-t641 cDNAs were subcloned into the pCMV-ARMS1-PK2 expression vector. These plasmids were transfected into PathHunter CHO-K1 Parental cells expressing β-arrestin 2 enzyme acceptor fusion protein and analyzed for agonist-induced cAMP response. The cAMP response in cells expressing eFSHR-t641 was lower than the response in cells expressing eFSHR-wt. EC₅₀ values of eFSHR-wt and eFSHR-t641 were 1079 ng/mL and 1834 ng/mL, respectively. eFSHR-t641 was approximately 0.58-fold compared with that of eFSHR-wt. The maximal response in eFSHR-wt and eFSHR-t641 was 24.7 nM and 16.7 nM, respectively. The Rmax value of phosphorylation sites in eFSHR-t641 was also decreased to approximately 68.4% of that in eFSHR-wt. The collective data implicate that the phosphorylation sites in the eFSHR C-terminal region have a pivotal role in signal transduction in PathHunter CHO-K1 cells, and indicate that β-arrestin is involved in coupling the activated receptors to the internalization system.

• 대한임상검사과학회지
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• 제39권1호
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• pp.7-18
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• 2007

A total of 1,780 isolates of Enterococcus spp. were isolated from 63,133 clinical specimens from Dec 1, 2005 to Nov 1, 2006 in "C" hospital. Isolation frequencies of Enterococcus spp. were 50.9% for E. faecalis, 41.7% for E. faecium, and 7.4% for other Enterococcus spp. containing E. avium, E. gallinarum, E. casseliflavus, E. durans, E. hirae, and E. raffinosus. There were no significant difference between gender, but according to the age group analysis, Enterococcus spp. were more frequently isolated in patients over 50 years old (20.0~24.6%) than those isolated from the patients under the age of 0~49 (1.3~9.4%). In monthly analysis, Enterococcus spp. were the most frequently isolated in April (11.9%), but presented at lowest frequency in February (5.2%). Seasonal analysis did not show a significant difference. Over half of enterococci were isolated from random urine (44.9%) and catherterized urine (15.7%). Frequencies of vancomycin resistant E. faecalis and E. faecium were 0.1% and 31.0%, respectively. Teicoplanin resistant Enterococcus was 13.3% in E. faecalis, 17.6 % in E. faecium. The Enterococcus species showing over 80% susceptibility against antimicrobial agents were E. faecalis, E. durans and E. hirae in vancomycin; E. faecalis, E. gallinarum, E. casseliflavus, E. durans and E. hirae in ampicillin. The antimicrobial agent showing susceptibility against whole group of Enterococcus species was only linezolid (95.9%), and a selection of antimicrobial agent is necessary to do essential performance identification and susceptibility tests.

• Nutritional Sciences
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• 제6권4호
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• pp.223-228
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• 2003

This study was carried out to investigate the effects of vitamin E on the cadmium contents of bone and on the calcium and phosphorous contents of the blood, urine and feces. Male Sprague-Dawley rats weighing 100$\pm$10g were randomly assigned to one normal group and three cadmium poisoned groups. The cadmium poisoned groups consisted of a vitamin E free diet (Cd-0E) group; a 40 mg vitamin E /kg diet (Cd-40E) group; and a 400 mg/kg diet (Cd-400E) group. Experimental animals were maintained on their respective diets for 20 weeks and were simultaneously administered 50 ppm $Cd^{2+}$ dissolved in the drinking water. At the end of the trial, the average hematocrit value in the Cd-0E group was 28.13% lower than in the normal group. However, the average hematocrit value in the Cd-400E group was significantly higher than in the Cd-0E and Cd-40E groups. WBC levels in the cadmium-poisoned groups were lower than in the normal group, but Cd-400E group levels were significantly higher than in the Cd-0E and Cd-40E groups. The contents of calcium of tibia has no significant difference between normal group and cadmium exposed group at $10^{th}$ week After 20 weeks, the calcium contents of the tibia in the Cd-0E and Cd-40E groups were lower than in the normal group by 25.5% and 22.1 %, respectively, although the calcium contents of the tibia in the Cd-400E group were higher than in the normal group. After 10 weeks, the calcium contents of the femur in the Cd-0E and Cd-40E groups were 19.25% and 15.45% lower than in the normal group, respectively, but the calcium contents of the femur in the Cd-400E group were at the same levels as in the normal group. The levels of calcium in the femur after 20 weeks were similar to the 10-week levels. Calcium levels of the urine in the Cd-0E and Cd-40E groups were 3.92 fold and 2.92 fold higher, respectively, than in the normal group, but levels in the Cd-400E group were significantly lower than in either the Cd-0E group or the Cd-40E group. Calcium levels of the feces in cadmium-poisoned groups were significantly higher than in the normal group, although levels in the Cd-400E group were significantly lower than in the Cd-0E and Cd-40E groups. Phosphorous levels of the blood in the Cd-0E group were 17% lower than in the normal group, although levels in the Cd-400E group were significantly higher than in the Cd-0E group. Phosphorous levels of the urine in the Cd-0E and Cd-40E groups were significantly higher than in the normal group, while Cd-400E group levels were found to be at the same level as in the normal group. Cadmium contents of the tibia in the Cd-40E and Cd-400E groups were 13% and 17% lower, respectively, than in the Cd-0E group. Regarding cadmium levels in the femur, only the Cd-400E group achieved lower levels (10% lower) than the Cd-0E group. In conclusion, vitamin E supplementation resulted in a suppression of the release of calcium from bone, and a reduction in the excretion of calcium via the urine and feces, thus having a normalizing effect on calcium metabolism in rats with chronic cadmium poisoning.

• 공학교육연구
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• 제15권4호
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• pp.109-117
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• 2012

The purpose of this study is to give the preliminary data and suggestion for introducing and spreading e-learning engineering education through analyzing learning behaviors, learning motivations, and satisfaction of e-learning engineering students. Especially, this comparative study analyzes each research domain according to majors and grades, thereby suggesting more specific and practical results. 2,745 students registered in 38 subjects of e-learning in 2 Universities were analyzed for this study. The study result shows that engineering students are attending around 2 e-learning subjects with a duration of about 30 minutes once a week. The main of learning motivation for e-learning was not easy test level and feasibility of acquiring credit but advantages of e-learning such as freedom of time and space, learning by repetition. The satisfaction scores of e-learning were lower compared to the aspects of system and contents Based on these results, first, an active spread of e-learning to engineering education is necessary because the demand from the engineering students is high enough and they have desirable learning behavior and learning motivation for it. Second, the characteristics of grades need to be taken into consideration on operation of e-learning. Third, a successful e-learning process needs more meticulous and active operation.