• Journal of Microbiology and Biotechnology
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• 제14권5호
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• pp.985-990
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• 2004

Levans produced from Microbacterium laevaniformans were isolated, characterized, and fractionated by molecular weight. TLC, HPLC, and GC-MS analyses of the exopolysaccharide showed that it was a fructan-type polymer and was composed of (2,6)- and (2,1)-glycosidic linkages. $^{13}C$-NMR analysis proved that the polysaccharide was mainly a $\beta$-(2,6)-linked levan-type polysaccharide. To investigate the cytotoxicity of the acetone-precipitated levan fractions such as M1, M2, and M3, HepG2, P388D1, U937, SNU-1, and SNUC2A cell lines were screened. Among the cell lines tested, the cytotoxicity of M1- M3 fractions were detected from only SNU-1 and HepG2 cells at the dosage level of $100-800\mu\textrm{g}ml$. The M2 fraction M_r$, 80,000) at 400 $mu{g/ml}$ had the greatest cell growth inhibition (84.6%) on SNU-1, while the M1 $(M_r$, 50,000) at $800\mu\textrm{g}ml$ showed the greatest (46.32%) on HepG2. To obtain more uniform M_r$ fractions of levan, the levan was further fractionated from S1 $(M_r$ 1,000,000) to S5 $(M_r$ 10,000) using gel permeation chromatography. Again, the S1-S5 fractions had strong cytotoxicity on SNU-1 and HepG2 cell lines. The greatest inhibition effects of S4 $(M_r$ 80,000) on SNU-1 and S5 $(M_r$ 10,000) on HepG2 were shown to be 49.5% and 73.0%, respectively. The cytotoxicity of the levan fractions was more effective on SNU-1 than on HepG2. Although the relationship between the Mw and the cytotoxicity was not clear, smaller $M_r$, fractions of levan showed greater growth inhibition effect on the cancer cell lines in general. Therefore, it was indicated that a specific Mw class of levan is responsible for the effective cytotoxicity.

• Journal of Pharmaceutical Investigation
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• 제39권1호
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• pp.73-78
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• 2009

A simple LC-MS/MS method of rabeprazole in human plasma was developed and validated. Rabeprazole and Internal standard (I.S), omeprazole, were extracted from human plasma by liquid liquid extraction, chromatographic separation of rabaprazole in plasma was achieved at $45^{\circ}C$ with a Shiseido UG120 $C_{18}$ column and methanol-10 mM ammonium acetate buffer (pH 9.42 with ammonium water), as mobile phase. Rabeprazole produced a protonated precursor ion [$(M+H)^+$] at m/z 360.10 and corresponding product ion at m/z 242.21. Internal standard produced a protonated precursor ion [$(M+H)^+$] at 346.09 and corresponding product ion at m/z 198.09. This method showed linear response over the concentration range of $1{\sim}500\;ng/mL$ with correalation coefficient greater than 0.99. The lower limit of quantitation (LLOQ) using 0.2 mL plasma was 1 ng/mL, which was sensitive enough for pharmacokinetics studies. The method was specific and validated with a limit of quantitation of 1 ng/mL. The intra-day and inter-day precision and accuracy were acceptable for all samples including the LLOQ. The applicability of the method was demonstrated by analysis of plasma after administration of a single 10 mg dose to 36 healthy subject. From the plasma rabeprazole concentration versus time curves, the mean $AUC_t$ (The area under the plasma concentration-time curve from time 0 to 12 hr ) was $691.36{\pm}321.88\;ng{\cdot}hr/mL$, $C_{max}$ (maximum plasma drug concentration) of $353.21{\pm}131.52\;ng/mL$ reached $3.4{\pm}1.1\;hr$ after adiministration. The mean biological half-life of rabeprazole was $1.37{\pm}0.75\;hr$. Based on the results, this simple method could readily be used in pharmacokinetics studies.

• 한국환경복원기술학회지
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• 제20권6호
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• pp.117-131
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• 2017

This study was conducted to draw vegetation landscape elements in the ancient literature, investigate plant community structure, understand vegetation structure, and suggest reasonal conservation management methods. As a result of analyzing ancient literature, geomorphological landscapes in Guryong valley were canyon landscape and valley surrounded in Mt. Chiak. Plant landscape factors were as followed. Rhododendron spp. spread around valley. Also, Pinus densiflora communities were distributed in Guryong valley around. Especially, the entrance zones of Guryong valley were described as covered with Pinus densiflora and Whangchangkumpyo(黃腸禁標). Therefore, it was estimated that entry of Guryong valley was covered with Pinus densiflora community landscape. As for current vegetation result, the main vegetation was divided into mixed deciduous broad-leaved trees community and Pinus densiflora community. As a result of analysis by TWINSPAN for community classification, five communities(Deciduous broadleaved trees, Pinus densiflora, Pinus densiflora-Abies holophylla, Abies holophylla, and Pinus koraiensis community) were classified. To maintain historic plant landscape and conserve crucial resources, Pinus densiflora community was classified as concentrated conservation management area($105,472m^2$). To maintain Pinus densiflora landscape which has high historical and cultural value of Guryong valley, it was considered that active density control of lower layer vegetation would be necessary. Accordingly, to conserve P. densiflora landscape, Whangchangkumpyo(?腸禁標), that area was separated into Pinus densiflora lower layer forest management area($84,029m^2$) and Pinus densiflora seedling conservation management area($21,443m^2$). In understory of Pinus densiflora lower layer flora, the target tree species for elimination and management were Quercus serrata and Quercus mongolica. They were $4{\sim}6trees/100m^2$ and their average diameter was 7.1cm. To preserve Pinus densiflora seedlings, areas with Sasa borealis, the ground vegetation of Pinus densiflora community, rate of 80% or more should be selected as priority management areas and concentrated elimination and management of Sasa borealis should be implemented. Likewise, traditional Pinus densiflora forest is a historically cultural heritage to preserve with sustainable interest and survey. Efficient management method through systematic monitoring system should be made.

• 한국산업융합학회 논문집
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• 제21권3호
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• pp.141-147
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• 2018

In a time when product development cycles are getting shorter and shorter, many companies are making efforts to develop products with high reliability in a short period of time, accelerated life test is widely used as a method to quickly evaluate reliability. Accelerated life test reduces the test life or the life of the product from the observed data by shortening the lifetime of the product or abruptly lowering the performance under the worse condition than the actual condition in order to shorten the test cost or the test time. In this paper, BL3640A-06P+RB35, DC12V model, which is used in the support device of an automatic rotation type digital signage, which display various information such as textures and images on a display screen in a public place or a commercial space, BLDC motors were subjected to a constant stress test and at the rotational speed of 1rpm, $180^{\circ}$ rotation and reverse rotation under actual use conditions, the stress was imposed on the rotating speed of 2rpm and the weight of the actual installed product from 22.2kgf to 10kgf were installed. The lifetime of the actual use environment condition is 23,545 hours and the rotation speed is accelerated. The life time of the acceleration condition with the additional weight is 1,380 hours. The acceleration factor is calculated as 17.06, the one year guarantee test day is 235 days to 14 days, of the period from 470 days to 28 days, and the third year from 704 days to 42 days. The test date of the BLDC motor was tested on the shortened test date, and the rotational speed and the current value were measured. It is found that there is no defect even if it operates as the test date corresponding to the specified one year warranty period and the 3 year accelerated life test which is experimented. Using the statistical technique of the regression analysis the expected time for the motor to defect to #4 samples was 20 years.

• 대한한의학방제학회지
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• 제25권2호
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• pp.179-191
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• 2017

Objectives : Increased oxidative stress by reactive oxygen species (ROS) has been suggested as a major cause of muscle fatigue. Although several studies have demonstrated the various biological properties of Sophora flavescens Aiton, Glycyrrhiza uralensis Fischer and Dictamnus dasycarpus Turcz, but the antioxidative potentials have not been clearly demonstrated. The present study was designed to investigate the protective effects of their water and ethanol extract mixtures (medicinal herbal mixtures, MHMIXs) on hydrogen peroxide ($H_2O_2$)-induced cell damage and apoptosis in C2C12 myoblasts. Methods : Cytotoxicity was assessed by an MTT assay. Quantitative evaluation of apoptosis induction and ROS production was evaluated by flow cytometry analysis. Expression levels of apoptosis regulatory and DNA-damage proteins were detected by Western blotting. Result : The inhibition of $H_2O_2$-induced cell proliferation was effectively blocked in extracts of 3: 1: 1 (EMHMIXs-1) or 2: 2: 1 (EMHMIXs-2) of S. flavescens, G. uralensis and D. dasycarpus Turcz, ethanol extracts from various complex extracts in C2C12 myoblasts. EMHMIXs-1 and EMHMIXs-2 also effectively attenuated $H_2O_2$-induced C2C12 cell apoptosis, which was associated with the restoration of the upregulation of Bad and death receptor 4, and downregulation of XIAP and cIAP-1 induced by $H_2O_2$. In addition, these herbal mixtures significantly blocked the $H_2O_2$-induced ROS generation and phosphorylation of $p-{\gamma}H2A.X$, which suggests that they can prevent $H_2O_2$-induced cellular DNA damage. Conclusions : The results suggest that EMHMIXs-1 and EMHMIXs-2 could block the DAN damage and apoptosis of C2C12 myoblasts by oxidative stress through blocking ROS generation.

• 여성건강간호학회지
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• 제8권1호
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• pp.124-137
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• 2002

The purpose of this study was to identify the need for sex education in middle school students Research subjects were 194 students who attended at I seminary in B city. The period of data collection was from July to August, 2001. Research instrument was composed of 61 structured items 17 items related to demographics and sex, 44 items related to the need of sex education. Data were collected through the self-report method by the researcher and one trained assistant. The instrument developed by the researchers was employed to measure the need of sex education and it's Cronbach's $\alpha$ = 0.9349 in this research. Data were analyzed by frequency, percentage factor analysis and t-test using SPSS WIN Program. The findings of this study are summarized as follows : 1. The highest score among characteristics of sex was 'less interest about sex(42.6%)', 39.0% of subjects got information about sex from friends or elders. 52.3% of subjects consulted friends or elders about sex problems. The number of sex education lessons was 1-2 times in school during last semester (61.0%). Education by the school nurse was 35.9% to students. 43.1% of subjects announced moderate satisfaction on sex education. 2. The mean score of the need for sex education was $2.88{\pm}0.47$ (lowest value 1, highest 4). The most wanted sex education factor was 'chastity and sexual responsibility($3.33{\pm}0.67$)' and followed by 'family and marriage ($3.26{\pm}0.62$)' and 'considerations of sex($3.07{\pm}0.69$)'. Meanwhile, the lowest wanted sex education factor is 'physiological characteristics of male ($2.47{\pm}0.72$)', followed by 'sexual behavior ($2.49{\pm}0.75$)'. The most wanted sex education item was 'the meaning of family ($3.54{\pm}0.75$)' and followed by 'the cautions to prevention from sexual abuse ($3.49{\pm}0.78$)' and sexual activity and responsibility($3.43{\pm}0.77$)'. Meanwhile, the lowest wanted sex education item is 'masturbation ($2.16{\pm}0.97$)', followed by 'circumcision($2.32{\pm}0.97$)'. 3. There is no significant difference between boys and girls in mean score about the need for sex education(t=-.715, p=.476). Sex education factor that girl-students wanted was 'physiological characteristics of female'. Sex education factor that boy-students wanted was 'family and marriage'. Sex education items that girl-students wanted were 'cautions during menstruation', the relation of ovulation and pregnancy', caution to prevention from sexual abuse' and sexual behavior and responsibility'. Sex education items that boy-students wanted were 'the meaning of family', 'the importance of marriage', 'male's sex organs', 'a wet dream', 'the method to resolve sexual curiosity', 'sexual intercourse' and 'the connection with the other sex'. In conclusion, the mean score of the need for sex education is medium. The route to attain knowledge about sex and sexual problem is through consulting friends and elders. Therefore, it is necessary to give sex education that is suitable to the subject's needs and methods. Also, it is necessary to give differently strong point about sex education between female and male students.

• Journal of Yeungnam Medical Science
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• 제5권1호
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• pp.69-75
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• 1988

대구시내 6개구에서 각 2개소의 아파트와 각 1개소의 단독주책을 선정하여 총 18개소의 가정상수를 1987년 5월 12일에서 10월 15일 사이에 봄, 여름, 가을 3회에 걸쳐 채취하여 이화학적 및 미생물학적 수질검사를 시행하였다. pH치는 최저 6.80에서 최고 8.10으로 모두 정상범위였으며, 잔류염소치 0.2ppm미만인 경우가 아파트군 16.7%, 단독주택군 5.6%였다. 아질산성 질소양성은 아파트군에서만 2.8%로 나타났다. 일반세균수가 $m{\ell}$당 100개 이상인 경우는 아파트군 11.1%, 단독주택군 11.1%였고, 대장균군의 MPN이 1.8%이상인 경우가 아파트군 27.8%, 단독주택군 33.3%였다. 잔류염소치, 아질산성 질소, 일반세균수 및 대장균군 검사에서 4가지 음용기준에 부적당한 것은 아파트군에서만, 2.7%였고, 3가지 검사에서 부적당한 것이 아파트군 2.7% 단독주택군 5.6%, 2가지 검사에서 부적당한 것이 아파트군 8.3% 단독주택군 5.6%, 1가지 검사에서 부적당한 것이 아파트군 25.0% 단독주택군 22.2%였다. 이화학적 및 미생물학적 검사성적의 상호비교에서 잔류염소치와 일반세균수 사이에, 그리고 대장균균수와 일반세균수 사이에 연관성이 있었다. 아파트의 건립년수가 길수록 수질이 좋지 않은 것으로 관찰되었다.

• 한국콘텐츠학회논문지
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• 제13권6호
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• pp.331-338
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• 2013

간질환을 주소로 추적검사를 위해 내원한 CT(Computer Tomography, 이하 CT) 검사자를 대상으로 체질량 지수와 관전압 변화에 따른 영상의 화질 및 방사선 피폭선량변화에 대하여 알아보고자 하였다. 2010년 3월부터 2011년 6월까지 부산 P대학병원에 복부 CT 를 검사한 환자 중 체질량지수(Body Mass Index, 이하 BMI)가 25이하인 환자를 대상으로 하였고 대상자는 48명이었다. 영상의 질의 객관적 평가로 신호대 잡음비와 유효선량을 비교하였다. 복부 영상의 화질평가는 영상의학과 의사2명이 한국의료영상품질관리원에서 선정한 임상영상 평가의 기준을 근거로 해 1점에서 20점까지 점수를 매겨 평가하였다. 피폭선량분석에서 CTDIvol값은 관전압이 100kVp일 때 120kVp보다 약44.1%가 감소하였다. 그리고 유효선량은 관전압 100kVp일 때 120kVp보다 약43%가 감소하였다. 영상의 화질 평가는 반복적으로 CT검사를 위해 내원한 총48명의 검사자 영상 중 Good 1명, Excellent 47명으로 평가되었다. 추적검사를 시행하는 환자 중 BMI지수가 25이하인 환자들을 대상으로 저관전압을 적용한 복부 CT검사 시 영상의 질적 저하없이 진단 가치가 있는 영상의 획득과 피폭선량 감소효과를 얻을 수 있다고 사료된다.

• Journal of Microbiology and Biotechnology
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• 제27권3호
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• pp.535-541
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• 2017

Two Gram-staining-negative, red-pinkish, coccus-shaped, non-motile, and aerobic bacterial strains, designated $Ant21^T$ and Ant22, were isolated from the Antarctic coastal sea water. Strains $Ant21^T$ and Ant22 showed UVC and gamma radiation resistance. Phylogenetic analyses based on 16S rRNA gene sequences determined that these strains belong to the genus Deinococcus. Through the analyses of the 16S rRNA gene sequences, strains $Ant21^T$ and Ant22 were found to have 97.7% and 97.8% similarity to Deinococcus marmoris DSM $12784^T$ and 97.0% and 97.2% similarity to Deinococcus saxicola AA-$1444^T$, respectively. The sequence similarity with the type strains of other Deinococcus species was less than 96.9% for both strains. Strains $Ant21^T$ and Ant22 shared relatively high 16S rRNA gene sequence similarity (99.3%) and had a closely related DNA reassociation value of $84{\pm}0.5%$. Meanwhile, they showed a low level of DNA-DNA hybridization (<30%) with other closely related species of the genus Deinococcus. The two strains also showed typical chemotaxonomic features for the genus Deinococcus, in terms of the major polar lipid (phosphoglycolipid) and the major fatty acids ($C_{16:0}$, $C_{16:1}$ ${\omega}6c/{\omega}7c$, $iso-C_{17:0}$, and $iso-C_{15:0}$). They grew at temperatures between $4^{\circ}C$ and $30^{\circ}C$ and at pH values of 6.0-8.0. Based on the physiological characteristics, the 16S rRNA gene sequence analysis results, and the low DNA-DNA reassociation level with Deionococcus marmoris, strains $Ant21^T$ ($=KEMB\;9004-167^T$ $=JCM\;31436^T$) and Ant22 (KEMB 9004-168 =JCM 31437) represent novel species belonging to the genus Deinococcus, for which the name Deinococcus rubrus is proposed.

• Journal of Microbiology and Biotechnology
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• 제30권4호
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• pp.526-532
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• 2020

A bacterial strain, designated B301^T and isolated from raw chicken meat obtained from a local market in Korea, was characterized and identified using a polyphasic taxonomic approach. Cells were gram-negative, non-motile, obligate-aerobic coccobacilli that were catalase-positive and oxidase-negative. The optimum growth conditions were 30℃, pH 7.0, and 0% NaCl in tryptic soy broth. Colonies were round, convex, smooth, and cream-colored on tryptic soy agar. Strain B301^T has a genome size of 3,102,684 bp, with 2,840 protein-coding genes and 102 RNA genes. The 16S rRNA gene analysis revealed that strain B301^T belongs to the genus Acinetobacter and shares highest sequence similarity (97.12%) with A. celticus ANC 4603^T and A. sichuanensis WCHAc060041^T. The average nucleotide identity and digital DNA-DNA hybridization values for closely related species were below the cutoff values for species delineation (95-96% and 70%, respectively). The DNA G+C content of strain B301^T was 37.0%. The major respiratory quinone was Q-9, and the cellular fatty acids were primarily summed feature 3 (C_16:1 ω6c/C_16:1 ω7c), C_16:0, and C_18:1 ω9c. The major polar lipids were phosphatidylethanolamine, diphosphatidyl-glycerol, phosphatidylglycerol, and phosphatidyl-serine. The antimicrobial resistance profile of strain B301^T revealed the absence of antibiotic-resistance genes. Susceptibility to a wide range of antimicrobials, including imipenem, minocycline, ampicillin, and tetracycline, was also observed. The results of the phenotypic, chemotaxonomic, and phylogenetic analyses indicate that strain B301^T represents a novel species of the genus Acinetobacter, for which the name Acinetobacter pullorum sp. nov. is proposed. The type strain is B301^T (=KACC 21653^T = JCM 33942^T).