Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Protective Effects of Ethanol Extract Mixtures of Sophora flavescens, Glycyrrhiza uralensis and Dictamnus dasycarpus against Oxidative Stress-induced Damage in C2C12 Murine Myoblasts

C2C12 근아세포의 산화적 손상에 대한 고삼, 감초 및 백선피 복합 추출물의 보호효과

  • Choi, Eun Ok (Anti-Aging Research Center, Dong-Eui University) ;
  • Hwang-Bo, Hyun (Anti-Aging Research Center, Dong-Eui University) ;
  • Kim, Min Young (Anti-Aging Research Center, Dong-Eui University) ;
  • Son, Da Hee (Department of Biochemistry, College of Korean Medicine, Dong-Eui University) ;
  • Jeong, Jin Woo (Anti-Aging Research Center, Dong-Eui University) ;
  • Park, Cheol (Departments of Molecular Biology, College of Natural Sciences, Dong-Eui University) ;
  • Hong, Su Hyun (Department of Biochemistry, College of Korean Medicine, Dong-Eui University) ;
  • Kim, Min Ju (Lioele Cosmetic Co., LTD.) ;
  • Lee, Ji Young (Lioele Cosmetic Co., LTD.) ;
  • Shin, Su Jin (Lioele Cosmetic Co., LTD.) ;
  • Choi, Yung Hyun (Anti-Aging Research Center, Dong-Eui University)
  • 최은옥 (동의대학교 항노화연구소) ;
  • 황보현 (동의대학교 항노화연구소) ;
  • 김민영 (동의대학교 항노화연구소) ;
  • 손다희 (동의대학교 한의과대학 생화학교실) ;
  • 정진우 (동의대학교 항노화연구소) ;
  • 박철 (동의대학교 자연생활과학대학 분자생물학과) ;
  • 홍수현 (동의대학교 한의과대학 생화학교실) ;
  • 김민주 ((주)리오엘리) ;
  • 이지영 ((주)리오엘리) ;
  • 신수진 ((주)리오엘리) ;
  • 최영현 (동의대학교 항노화연구소)
  • Received : 2017.05.16
  • Accepted : 2017.05.29
  • Published : 2017.05.31
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Abstract

Objectives : Increased oxidative stress by reactive oxygen species (ROS) has been suggested as a major cause of muscle fatigue. Although several studies have demonstrated the various biological properties of Sophora flavescens Aiton, Glycyrrhiza uralensis Fischer and Dictamnus dasycarpus Turcz, but the antioxidative potentials have not been clearly demonstrated. The present study was designed to investigate the protective effects of their water and ethanol extract mixtures (medicinal herbal mixtures, MHMIXs) on hydrogen peroxide ($H_2O_2$)-induced cell damage and apoptosis in C2C12 myoblasts. Methods : Cytotoxicity was assessed by an MTT assay. Quantitative evaluation of apoptosis induction and ROS production was evaluated by flow cytometry analysis. Expression levels of apoptosis regulatory and DNA-damage proteins were detected by Western blotting. Result : The inhibition of $H_2O_2$-induced cell proliferation was effectively blocked in extracts of 3: 1: 1 (EMHMIXs-1) or 2: 2: 1 (EMHMIXs-2) of S. flavescens, G. uralensis and D. dasycarpus Turcz, ethanol extracts from various complex extracts in C2C12 myoblasts. EMHMIXs-1 and EMHMIXs-2 also effectively attenuated $H_2O_2$-induced C2C12 cell apoptosis, which was associated with the restoration of the upregulation of Bad and death receptor 4, and downregulation of XIAP and cIAP-1 induced by $H_2O_2$. In addition, these herbal mixtures significantly blocked the $H_2O_2$-induced ROS generation and phosphorylation of $p-{\gamma}H2A.X$, which suggests that they can prevent $H_2O_2$-induced cellular DNA damage. Conclusions : The results suggest that EMHMIXs-1 and EMHMIXs-2 could block the DAN damage and apoptosis of C2C12 myoblasts by oxidative stress through blocking ROS generation.

Keywords

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