• Journal of Life Science
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• v.8 no.5
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• pp.604-613
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• 1998

The last enzyme of the sesquiterpen phytoalexin capsidiol synthesis in tobacco cell, 5-epi-aristolochene hydro-xylase which convert 5-epi-aristolochene (EAS) to capsidiol, was cloned by a reverse transcription polymerase chain reaction strategy and cDNA library screening. Cloned CYP-B3 contained high probability amino acid matches to known plant cytochrome P450 sequences and open reading frame with the conserved FxxGxRxCxG heme-binding region. Transcripts of CYP-B3 were not detected in control cells, but induced in elicitor-treated cells. Furthermore, CYP-B3 transcripts were induced by fungal extracts and cellulase but not by other stimuli(chilling, heat shock and 2,4-D). Induction of CYP-B3 transcripts by elicitor treatment was not affected by ancymidol and ketoconazole treat-ments suggesting that an inhibition of hydroxylase activity by Cyt P450 inhibitors resulting from post translational processing event.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.160-166
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• 2008

A DNA fragment containing 2,079 base pairs from Bacillus circulans CGMCC 1416 was cloned using degenerate PCR and inverse PCR. An open reading frame containing 981 bp was identified that encoding 326 amino acids residues, including a putative signal peptide of 31 residues. The deduced amino acid sequence showed the highest identity (68.1%) with $endo-{\beta}-1,4-D-mannanase$ from Bacillus circulans strain K-1 of the glycoside hydrolase family 5 (GH5). The sequence encoding the mature protein was cloned into the pET-22b(+) vector and expressed in Escherichia coli as a recombinant fusion protein containing an N-terminal hexahistidine sequence. The fusion protein was purified by $Ni^{2+}$ affinity chromatography and its hexahistidine tag cleaved to yield a 31-kDa ${\beta}$-mannanase having a specific activity of 481.55U/mg. The optimal activity of the purified protein, MANB48, was at $58^{\circ}C$ and pH 7.6. The hydrolysis product on substrate locust bean gum included a monosaccharide and mainly oligosaccharides. The recombinant MANB48 may be of potential use in the feed industry.

• Proceedings of the KAIS Fall Conference
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• 2011.05a
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• pp.122-125
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• 2011

본 연구에서는 기존 교육시설에 설치된 알루미늄 합금 창의 2차원 정상상태 전열해석을 통한 열성능 평가를 수행하였다. 교육시설의 주요 창호재료로 적용된 알루미늄은 열전도율이 $175 \;Kcal/m^2h^{\circ}C$ 정도로 플라스틱 소재와 비교하여 매우 불리하여 기존 시설에 설치된 알루미늄 창호는 열손실의 주요인으로 지적되고 있다. 본 연구에서는 이러한 점에 착안하여 충남 서산지방에 위치한 대학건물의 알루미늄 합금 창호의 열성능 평가에 관한 연구를 수행하여 다음과 같은 해석 결과를 도출하였다. (1) 2차원 정상상태 전열해석을 위한 경계조건은 국토해양부고시 건축물의 에너지절약설계기준의 [별표 6] 중부지방 냉난방장치의 용량계산을 위한 설계 외기온 기준과 [별표 7]의 실내온도 기준을 적용하여 여름철 실내 $27^{\circ}C$, 실외 $31.3^{\circ}C$, 겨울철 실내 $21.0^{\circ}C$, 실외 $-9.6^{\circ}C$ 로 설정하고 해석한 결과 열관류율은 알루미늄 합금 창호는 $U=9.631 \;W/m^2K$, 복층유리 $U= 2.382 \;W/m^2K$로 여름철과 겨울철 동일한 해석결과치가 산출되었다. (2) 산출된 열관류율 해석결과를 건축물의 에너지절약설계기준 [별표 3] 열교차단재가 적용되지 않은 금속제 창의 단열성능 중 일반복층창 성능기준인 $4.0 \;W/m^2K$와 비교할 때, 알루미늄 창틀을 통하여 225%의 열량이 손실됨을 보여 주고 있다.

• Journal of architectural history
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• v.23 no.1
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• pp.7-19
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• 2014

This study considers the proper repair techniques by examining the most representative repair cases of the Korean arch bridges and proposes the constructional manual which can apply similar occasions. The cases are Seonamsa Seungseongyo and Songgwangsa Geukrockgyo where this researcher had taken part in the repair works. This Study proposes the maintenance construction manual about the performance degradation drew by performance degradation of the both Korean arch bridges in the maintenance process. First, arch bridge maintenance should be carried out in the dry season, when water is impermeable in the bottom surface of the bridge. Moreover, risk factors of the maintenance should be excluded to secure the water vally flow, the bypass and the temporary bridge. Second, prior to repair, it has to precede (1)3D shooting (2)formal examination (3)structure safety test (4)geological and lithic surveys (5)arch curvature establishment and makeshift frame settlement before transformation (6)relationship expert comments. Third, if the baduk and the foundation stones are inevitable to replace due to performance degradation on the foundation, it should use the high quality stones and secure greater stress by extending the standard range. The foundation on irregular rock needs to be flattened and underside on the replaced materials require Grengyijil to deliver the equal loads. Fourth, In the process of dismantling the stones of the arched bridge, it could make heavy weathering degree and not reuse the materials. Charge should converge the expert advices to choose the reuseable, the conservate and the alternative materials, and increase the reutilization of the raw materials by preservation and reinforcement treatments. Fifth, the side wall should be repaired by the rubble work technique which is not able to pile compost satiety, so it must use long depth of masonary stones for reinforcement. It is considered to reinforce the stone wall in shore as much as possible and protect the abutment and the side wall on the upstream for the arch bridge maintenance works.

• Journal of the Korean Society of Safety
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• v.20 no.4 s.72
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• pp.54-62
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• 2005

This Study is to find out how RC beam mixed with sawdust acts comparing with normal beam without sawdust mixture, and how they can be applied to the actual structural frame, despite a Int that they are mixed with waste material : saying sawdust. ED3H1, ED3H2, ED5H1, ED5H2, ED3H1UB, ED5H1UB, ED3H2L, ED5H2L and Normal without sawdust mixture are main factor to be tested here in order to apply them to the actual case. D and H means diameter 3cm or 5cm, and holes one and two respectively. And all variables are tested with each two for one variables. Test results are compared using crack diagrams and strain & loads. There are eleven(11)% capacity decrease between ED 3H1 and ED5H1 in rebar, strain. Left and right side crack shapes are much similar in variable ED3H2L having maximum capacity 14.5 tone. ED5H2L having maximum capacity thirteen(13)tone, in case of normal 19.6 tone. Two holes in beam rather on the longitudinal direction than on the forcing direction can be more effective to keep the original capacity of the beam because this case can distribute load more uniformly. There is 33% capacity decreased in case of diameter five(5)cm, compared to diameter three(3)cm. Two holes give thirty nine(39) percent capacity decrease than one of diameter three(3)cm.

• Journal of Navigation and Port Research
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• v.41 no.6
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• pp.381-388
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• 2017

Recently, renewed attention for technological applications within the maritime industry has taken place, particularly regarding unmanned vessels. There has been a lot of interest about the number of projects aiming at testing the operational feasibility of unmanned vessels, particularly in relation to technological, safety and security issues. Nevertheless, no studies have investigated this issue from the point of view of seafarers towards unmanned vessels; this paper aims at filling this gap. Data has been collected through a survey questionnaire. A Pearson correlational coefficient has been used to test the correlation between some of the variables. The results show that more than half of the respondents indicated that unmanned vessels do not contribute to an increase in accidents at sea. In addition, seafarers believe unmanned vessels will not be operational in the near future, with most of the respondents indicating a 10-20 year time frame. Thirdly, most of the respondents(both officers and ratings).

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.11
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• pp.7582-7588
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• 2015

Noncoherent receivers are favored for block-code-modulated ultrawideband impulse radio (UWB-IR) systems because of their low implementation complexity compared with coherent rake receivers. However, existing noncoherent schemes, such as transmitted reference (TR) systems and averaged differential receivers (ADR), suffer from performance degradation and energy efficiency loss. Codeword matching and signal aggregation (CMSA) is a low complexity noncoherent receiver for UWB-IR. As the frame/symbol duration is shortened to boost data rate, interframe interference (IFI) or intersymbol interference (ISI) occurs and degrades the detection performance of CMSA. In this paper, block coded correlator which consists of the delay components and the reference signal is proposed to improve the performance of the receiver. Simulation results show that the proposed system leads to the better performance compared to the conventional CMSA receiver.

• Earthquakes and Structures
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• v.18 no.6
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• pp.677-689
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• 2020

A building structural system of moment resisting frame (MRF) with concrete filled steel tubular (CFST) columns and wide flange H beams, is one of the most conveniently constructed structural systems. However, there were few studies on evaluating seismic performance of full-scale CFST columns under high axial compression. In addition, some existing famous design codes propose various limits of width-to-thickness ratio (B/t) for steel tubes of the ductile CFST composite members. This study was intended to investigate the seismic behavior of CFST columns under high axial load compression. Four full-scale square CFST column specimens with a B/t of 42 were carried out that were subjected to horizontal cyclic-reversal loads combined with constantly light, medium and high axial loads and with a linearly varied axial load, respectively. Test results revealed that shear strength and deformation capacity of the columns significantly decreased when the axial compression exceeded 0.35 times the nominal compression strength of a CFST column, P₀. It was obvious that the higher the axial compression, the lower both the shear strength and deformation capacities were, and the earlier and faster the shear strength degradation occurred. It was found as well that higher axial compressions resulted in larger initial lateral stiffness and faster degradation of post-yield lateral stiffness. Meanwhile, the lower axial compressions led to better energy dissipation capacities with larger cumulative energy. Moreover, the study implied that under axial compressions greater than 0.35P₀, the CFST column specimens with B/t limits recommended by AISC 360 (2016), ACI 318 (2014), AIJ (2008) and EC4 (2004) codes do not provide ultimate interstory drift ratio of more than 3% radian, and only the limit in ACI 318 (2014) code satisfies this requirement when axial compression does not exceed 0.35P₀.

• Journal of Sericultural and Entomological Science
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• v.50 no.2
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• pp.189-193
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• 2012

To find diapause-related genes, we were performed by differential hybridization with three types of [${\alpha}-^{32}P$]dCTP-labeled total cDNA probes synthesized from diapause-prepared, diapause-maintained and diapause-activated stage of Bombus ignitus queen. Nine individual cDNA clones were found to be differentially expressed in diapause-maintained and diapause-activated stage. Among these clones, BIDC9(BIDC ; Bombus ignitus differentially expressed clone) was analyzed through full-length sequencing and expression pattern analysis. This clone was specifically expressed in the thorax organ. The effect of Juvenile hormone analog(JHA) and $CO_2$ treatment was examined. JHA treatment induced the expression of BIDC9 cDNA clone abruptly after 4 day of treatment. $CO_2$ treatment induced also the clone after 2 day of treatment. BIDC9 cDNA was identified as Bombus ignitus diapause gene contained an open reading frame of 1376 bp encoding 255 amino acids.

• Computers and Concrete
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• v.33 no.3
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• pp.251-264
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• 2024

The present study pertains to the effects of variations in the location and size of drilled web openings on the behavior of fixed-fixed reinforced concrete (RC) beams. For this purpose, a reference bending beam with a transverse opening in each half span was tested to failure. Later, the same beam was modeled and analyzed with the help of finite element software using ABAQUS. Upon achieving close agreement between the experimental and numerical results, the location and size of the web opening were altered to uncover the effects of these factors on the shear strength and load-deflection behavior of RC beams. The experimental failure mode of the tested beam and the numerical results were also verified by theoretical calculations. In numerical analysis, when compared to the reference (D0) specimen, if the distance of the opening center from the support is 0 or h or 2h, reduction in load-bearing capacity of 1.5%-22.8% or 2.0%-11.3% or is 4.1%-40.7%. In other words, both the numerical analyses and theoretical calculations indicated that the beam behavior shifted from shear-controlled to flexure-controlled as the openings approached the supports. Furthermore, the deformation capacities, energy absorption values, and the ductilities of the beams with different opening diameters also increased with the decreasing distance of the opening from supports. Web compression failure was shown to be the predominant mode of failure of beams with large diameters due to the lack of sufficient material in the diagonal compression strut of the beam. The present study indicated that transverse openings with diameters, not exceeding about 1/3 of the entire beam depth, do not cause the premature shear failure of RC beams. Finally, shear damage should be prevented by placing special reinforcements in the areas where such gaps are opened.