• Asian-Australasian Journal of Animal Sciences
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• v.31 no.4
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• pp.489-498
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• 2018

Objective: Foot and mouth disease (FMD) and porcine reproductive and respiratory syndrome (PRRS) are major diseases that interrupt porcine production. Because they are viral diseases, vaccinations are of only limited effectiveness in preventing outbreaks. To establish an alternative multi-resistant strategy against FMD virus (FMDV) and PRRS virus (PRRSV), the present study introduced two genetic modification techniques to porcine cells. Methods: First, cluster of differentiation 163 (CD163), the PRRSV viral receptor, was edited with the clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9 technique. The CD163 gene sequences of edited cells and control cells differed. Second, short hairpin RNA (shRNAs) were integrated into the cells. The shRNAs, targeting the 3D gene of FMDV and the open reading frame 7 (ORF7) gene of PRRSV, were transferred into fibroblasts. We also developed an in vitro shRNA verification method with a target gene expression vector. Results: shRNA activity was confirmed in vitro with vectors that expressed the 3D and ORF7 genes in the cells. Cells containing shRNAs showed lower transcript levels than cells with only the expression vectors. The shRNAs were integrated into CD163-edited cells to combine the two techniques, and the viral genes were suppressed in these cells. Conclusion: We established a multi-resistant strategy against viral diseases and an in vitro shRNA verification method.

• Journal of the Society of Naval Architects of Korea
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• v.42 no.6 s.144
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• pp.619-630
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• 2005

A two-frame PIV (Particle Image Velocimetry) technique is used to investigate the wake characteristics behind a marine propeller with 4 blades at high Reynolds number. For each of 9 different blade phases from $ 0^{\circ} $ to $ 80^{\circ} $, one hundred and fifty instantaneous velocity fields are measured. They are ensemble averaged to study the spatial evolution of the propeller wake in the region ranging from the trailing edge to one propeller diameter (D) downstream location. The phase-averaged mean velocity shows that the trailing vorticity is related to radial velocity jump, and the viscous wake is affected by boundary layers developed on the blade surfaces and centrifugal force. Both Galilean decomposition method and vortex identification method using swirling strength calculation are very useful for the study of vortex behaviors En the propeller wake legion. The slipstream contraction occurs in the near-wake region up to about X/D : 0.53 downstream. Thereafter, unstable oscillation occurs because of the reduction of interaction between the tip vortex and the wake sheet behind the maximum contraction point.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38A no.9
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• pp.810-816
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• 2013

In this paper, we propose a SSD (simultaneous single-band duplex) system using Digital Cancellation. Also, we propose a method for Digital Cancellation when RF Cancellation is effectively performed. The proposed system has estimation frame for effective self-interference channel estimation in time-domain. The proposed system calculates signal power for selection of optimal coefficient after digital cancellation. Then, the proposed system selects coefficient of minimum signal power. Further, the proposed system uses LDPC code to minimize the effects of remaining self-interference signal. The proposed system shows BER performance of at 20dB by cancelling self-interference and iterating LDPC code. That is, the proposed system shows that the SSD communication is possible in static self-interference channel.

• Journal of the Korea Society of Computer and Information
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• v.11 no.4 s.42
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• pp.77-85
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• 2006

There is the spatial correlation of the video sequence between the motion vector of current blocks. In this paper, we propose the enhanced fast block matching algorithm using the spatial correlation of the video sequence and the center-biased properly of motion vectors. The proposed algorithm determines an exact motion vector using the predicted motion vector from the adjacent macro blocks of the current frame and the Cross-Hexagonal search pattern. From the of experimental results, we can see that our proposed algorithm outperforms both the prediction search algorithm (NNS) and the fast block matching algorithm (CHS) in terms of the search speed and the coded video's quality. Using our algorithm, we can improve the search speed by up to $0.1{\sim}38%$ and also diminish the PSNR (Peak Signal Noise Ratio) by at nst $0.05{\sim}2.5dB$, thereby improving the video qualify.

• Investigative Magnetic Resonance Imaging
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• v.4 no.1
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• pp.7-13
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• 2000

Purpose : To assess the distortion of MRI with the Leksell stereotactic radiosurgery system in variable pulse sequence and imaging plane through phantom study, to find most adequate imaging plane and pulse sequence for stereotactic radiosurgery system. Materials and methods : We made the phantoms for MRI and get images in variable conditions and analyzed the image distortion using image analysis program, and statistically using paired student t-test. Results : The transeverse plane images had acceptable error ranges bless than 1.5mm) in all pulse sequence in both the analysis of fiducial marker in stereotactic G-frame and the phantom study. The coronal plane images had unacceptable large errors (more than 1.7mm) in the analysis of fiducial marker in the stereotactic G-frame, but had corrected small errors (less than 1.5mm) in the phantom study. Conclusion : We find from the phantom study that the present MR machines are adequate for stereotactic surgery system in frequently used pulse sequences, and imaging planes.

• Journal of Astronomy and Space Sciences
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• v.30 no.4
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• pp.269-277
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• 2013

In this study, we present results of precise orbital geodetic parameter estimation using satellite laser ranging (SLR) observations for the International Laser Ranging Service (ILRS) associate analysis center (AAC). Using normal point observations of LAGEOS-1, LAGEOS-2, ETALON-1, and ETALON-2 in SLR consolidated laser ranging data format, the NASA/GSFC GEODYN II and SOLVE software programs were utilized for precise orbit determination (POD) and finding solutions of a terrestrial reference frame (TRF) and Earth orientation parameters (EOPs). For POD, a weekly-based orbit determination strategy was employed to process SLR observations taken from 20 weeks in 2013. For solutions of TRF and EOPs, loosely constrained scheme was used to integrate POD results of four geodetic SLR satellites. The coordinates of 11 ILRS core sites were determined and daily polar motion and polar motion rates were estimated. The root mean square (RMS) value of post-fit residuals was used for orbit quality assessment, and both the stability of TRF and the precision of EOPs by external comparison were analyzed for verification of our solutions. Results of post-fit residuals show that the RMS of the orbits of LAGEOS-1 and LAGEOS-2 are 1.20 and 1.12 cm, and those of ETALON-1 and ETALON-2 are 1.02 and 1.11 cm, respectively. The stability analysis of TRF shows that the mean value of 3D stability of the coordinates of 11 ILRS core sites is 7.0 mm. An external comparison, with respect to International Earth rotation and Reference systems Service (IERS) 08 C04 results, shows that standard deviations of polar motion $X_P$ and $Y_P$ are 0.754 milliarcseconds (mas) and 0.576 mas, respectively. Our results of precise orbital and geodetic parameter estimation are reasonable and help advance research at ILRS AAC.

• 대한공업교육학회지
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• v.33 no.1
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• pp.191-212
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• 2008

The purpose of this study was to show how to organize small groups on the project method class in technical high school. The subjects of this study were two classes including 40 second-year students (20 students in each class) of architecture design course in D technical high school located in Taejeon metropolitan city. The method of this study was to investigate the ego-state by the ego-gram practicing Transactional Analysis. Total 10 small groups were organized. Each group had 4 members. And the same quality of egostate small group was organized by the 4 students who have high score on NP and AC. The different in kind small group was organized by the 4 students, each of whom has NP leading, A leading, FC leading and AC leading score. After the target small group was video- taped, students' verbal interaction was written into transcripts. Their individual states were analysed by the frame of verbal interaction. The result of this study was that the same quality of TAOK small group had more active interaction(110 times) than different in kind small group(87 times). The frequency of complementary transaction was also higher plenty in the same quality of TAOK small group.

• Journal of Biosystems Engineering
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• v.33 no.2
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• pp.83-93
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• 2008

In order to save labor and cost, direct seeding has been considered as an important alternative to the machine transplanting in rice cultivation. Current direct seeding machines for rice in Korea drill irregularly under various operating conditions. This study was conducted to develope a precision seeder which enables the accurate, even-spaced in row placement of rice seeds at uniform depths of 3-4 cm on dry paddy. Design, construction and performance evaluation of the precision seeder were carried out. The tractor rear-mounted type 8-rows precision seeder which performs seeding in addition to fertilizing, ditching, and rotary tilling works on dry paddy was developed. Main components of the seeder were ditcher and leveller, rotary tiller, powered roller type furrow opener, seeding device, powered roller type furrow covering and firming device, hydraulic unit, seeding speed control system, power transmission system, hitch and frame. Ditching, furrow opening, and seed covering and firming performances were good and seeding depths of 2-4 cm could be maintained. Planting accuracies and planting precisions were within 13.6%, and 31.2%, respectively, for planting space of 15 cm, and seeding velocity of 0.5 m/s. These mean variations of average planting space were within 2.1 cm, and 90% of seeds in a hill were seeded within 4.7 cm of hill length, respectively. Error ratios between setting planting space and measured average planting space were shown within 6.7%. Therefore the seeder showed good planting performance up to seeding velocity of 0.5 m/s in field tests. And field capacity of the seeder was about 0.28 ha/hour.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.45 no.9
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• pp.71-78
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• 2008

In this paper, we implement the H.264/AVC baseline decoder by hardware-software partitioning under the embedded Linux Kernel 2.4.26 and the FPGA-based target board with ARM926EJ-S core. We design several IPs for the time-demanding blocks, such as motion compensation, deblocking filter, and YUV-to-RGB and they are communicated with the host through the AMBA bus protocol. We also try to minimize the number of memory accesses between IPs and the reference software (JM 11.0) which is ported in the embedded Linux. The proposed IPs and the system have been designed and verified in several stages. The proposed system decodes the QCIF sample video at 2 frame per second when 24MHz of system clock is running and we expect the bitter performance if the proposed system is designed with ASIC.

• Journal of Animal Environmental Science
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• v.10 no.3
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• pp.155-162
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• 2004

The livestock liquid manure is one of important source for production of friendly environmental crops and have been used widespreadly in recent years. This survey is to eventually investigate the actual conditions of liquid manure utilization for cultivation of crops in the agricultural farm, based on the survey for 61 selected farms in 8 provinces(except Jeju province) included 22 counties in Korea. The results obtained in this survey were summarized as follow; $72.1\%$ of liquid manure storage tank(44) was located in the farmland and $27.9\%$(17) was in the fm. Most of liquid manure tank volume and material were 200 M/T($67.2\%$) and Polyethylene Double Frame panel($44.3\%$). The pro-portions of liquid manure application land were $45.9\%$ for rice paddy, $36.1\%$ for dry field, $16.4\%$ for orchard and $1.6\%$ for other, respectively. The controversial points of liquid manure utilization were malodor($60.7\%$), equipment possession($22.9\%$), no problem($13.1\%$) and farmland possession ($2.3\%$), respectively.