• KSBB Journal
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• 제27권2호
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• pp.97-102
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• 2012

In the present study, the biomass productivity of two green microalgae (Chlorella sp. and Dunaliella salina DCCBC2) were assessed in a 12 L tubular photobioreactor under optimum culture conditions. In the batch culture optimization process, the Chlorella sp. biomass was obtained as 1.2 g/L under atmospheric air as a sole $CO_2$ source and other culture conditions as follows: light intensity, temperature, pH, $NH_4Cl$ and $K_2HPO_4$ were 100 ${\mu}E/m^2/s$, $27^{\circ}C$, 7.0, 20.0 mM and 2.0 mM, respectively. On the other hand, 2.9 g/L of D. salina DCCBC2 biomass production was observed under the following conditions: light intensity, temperature, pH, $KNO_3$ and $K_2HPO_4$were 80 ${\mu}E/m^2/s$, $27^{\circ}C$, 8.0, 3.0 mM and 0.025 mM, respectively. At 1% $CO_2$ supply to the reactor, the Chlorella sp. production was reached 1.53 g/L with 25% increment under the same operating conditions. In addition, the maximum D. salina DCCBC2 biomass was observed as 3.40 g/L at 3% $CO_2$ concentration. Based on the aforementioned optimized conditions, the dilution rate and maximal biomass productivity of Chlorella sp. and D. salina DCCBC2 in the continuous cultivation were 0.4/d and 0.6 g/L/d and 0.6/d and 1.5 g/L/d, respectively.

• International Journal of Advanced Culture Technology
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• 제7권1호
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• pp.243-247
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• 2019

The most commonly used 3D modeling file formats are OBJ (Wavefront file format specification) and STL (STereoLithography). Although they have a common point of view in 3D on the screen, detailed functions are different according to purpose of development. OBJ is the most commonly used 3D file format and STL is mainly used as 3D file format for 3D printing. However, in the field of Scientific Visualization, precise analysis is required. There is a difference in accuracy depending on the type of 3D file format. OBJ and STL are not suitable for delicate surface description because they form meshes in the form of triangular polygons. And if you increase the number of triangle polygons, it will be smoother, but the file size also increases exponentially and causes excessive CPU usage. In contrast, VTK provides a variety of polygon structures, including triangular polygons as well as rectangular polygons and cube polygons. Thus, delicate surface depiction is possible. Delicate surface rendering is possible and file size is not large. This paper describes the concept and structure of VTK. We also compared the load times and file sizes between VTK, STL, and OBJ in the Chrome browser. In addition, the difference in surface rendering ability between VTK, STL, and OBJ is intuitively viewed based on the screen in which each 3D file format is implemented under the same conditions. This study is expected to be helpful for efficient 3D file format for precise implementation of Web - based Scientific Visualization.

• International Journal of Advanced Culture Technology
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• 제10권3호
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• pp.339-345
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• 2022

In this paper, the effect of porosity on the acoustic phase velocity of the 3D printed Kelvin closed-cell structure was investigated using the spectral phase analysis. Since Kelvin cells bring about the large amount of scattering, acoustic pulses in ultrasonic measurements undergoes a distortion of waveforms due to the dispersion effect. In order to take account on the dispersion, mathematical expressions for calculating the phase velocity of longitudinal waves propagating normal to the plane of the Kelvin structure are suggested by introducing a complex wave number based on Fourier transform. 3D Kelvin structure composed of identical unit-cells, a polyhedron of 14 faces with 6 quadrilateral and 8 hexagonal faces, was developed and fabricated by 3D CAD and 3D printer to represent the micro-structure of porous materials such as aluminum foam and cancellous bone. Total nine samples of 3D Kelvin structure with different porosity were made by changing the thickness of polyhedron. Ultrasonic pulse of 1MHz center frequency was applied to the Kelvin structures for the measurement of the phase velocity of ultrasound using the TOF(time-of-flight) and the phase spectral method. From the experimental results, it was found that the acoustic phase velocity decreased linearly with the porosity.

• 한국의류학회지
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• 제34권11호
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• pp.1836-1843
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• 2010

This research examines the possibilities of a traditional costume revival and digital exhibition as well as the application of 3D virtual clothing modeling data in order to preserve and record a disappearing costume heritage to realize it as a social education tool through the newly emerging technology of 3D virtual clothing. A 3D revival of costumes worn by royal families and aristocrats was accomplished through the 3D animation and simulation technology of Maya 2011 (Autodesk, Inc.) and Qualoth (FX Gear, Inc.). The simulation shows the possibility of a 3D revival and digital exhibition of costume heritage. However, further technology support to analyze and realize the composition and design is still necessary to develop the digital contents of traditional garment culture that includes skirt pleats, petticoat silhouettes, that exaggerates the skirts and knots of traditional upper garments (Jeogori). Further studies on design attributes of historic costumes and the upgrading of 3D simulation software are required to realize 3D virtual clothing. Korean traditional costumes will be revived as a cultural content in the digital era as a result of outstanding issues detected by this study.

• 복식문화연구
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• 제23권4호
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• pp.616-627
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• 2015

The purpose of this study was to develop slacks patterns for obese-schoolgirls aged 10~12 by using a 3D virtual garment simulation system. The criteria for subjects in this study were girls who had a BMI of over $25kg/m^2$. A total of 155 schoolgirls who met these criteria were enrolled. The results were as follows: First, by using 3D virtual garment simulation, a new slacks pattern considerate of obese-schoolgirls was developed. The basic numerical formulae were as follows: Front and back hip girth of H/4-0.5+1 and H/4+0.5+1.5, front waist girth of W/4+1+0.5, back waist girth of W/4+2+0.5, front crotch extension of H/16-0.5, back crotch extension of H/8-0.5, front dart amount of 1, and back dart amount of 2. Second, according to the new slacks pattern appearance evaluation, the new slacks pattern scored more highly than the existing pattern for silhouette and ease amount, confirming that the new slacks pattern is appropriate for obese-schoolgirls. Additionally, the new slacks pattern was evaluated allowing for the proper space length of the waist, abdomen and hips. This study is expected to serve as important basic data for ensuing studies that may utilize a 3D virtual garment simulation system with 2D patterns and for future 3D pattern production program development.

• Journal of Veterinary Science
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• 제24권1호
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• pp.4.1-4.16
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• 2023

Background: In vitro culture of preantral follicles is a promising technology for fertility preservation. Objectives: This study aims to investigate an optimized three-dimensional (3D) fetal bovine serum (FBS)-free preantral follicle culture system having a simple and easy operation. Methods: The isolated follicles from mouse ovaries were randomly divided in an ultra-low attachment 96-well plates supplement with FBS or bovine serum albumin (BSA) culture or encapsulated with an alginate supplement with FBS or BSA culture. Meanwhile, estradiol (E₂) concentration was assessed through enzyme-linked immunosorbent assay of culture supernatants. The diameter of follicular growth was measured, and the lumen of the follicle was photographed. Spindle microtubules of oocytes were detected via immunofluorescence. The ability of oocytes to fertilize was assessed using in vitro fertilization. Results: The diameters were larger for the growing secondary follicles cultured in ultra-low attachment 96-well plates than in the alginate gel on days 6, 8, and 10 (p < 0.05). Meanwhile, the E2 concentration in the BSA-supplemented medium was significantly higher in the alginate gel than in the other three groups on days 6 and 8 (p < 0.05), and the oocytes in the FBS-free system could complete meiosis and fertilization in vitro. Conclusions: The present study furnishes insights into the mature oocytes obtained from the 3D culture of the preantral follicle by using ultra-low attachment 96-well plate with an FBS-free system in vitro and supports the clinical practices to achieve competent, mature oocytes for in vitro fertilization.

• Journal of Microbiology and Biotechnology
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• 제19권5호
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• pp.474-481
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• 2009

A novel microorganism, designated as LG12, was isolated from soil based on its ability to use acrylic acid as the sole carbon source. An electron microscopic analysis of its morphological characteristics and phylogenetic classification by 16S rRNA homology showed that the LG12 strain belongs to Rhodococcus erythropolis. R. erythropolis LG12 was able to metabolize a high concentration of acrylic acid (up to 40 g/l). In addition, R. erythropolis LG12 exhibited the highest acrylic acid-degrading activity among the tested microorganisms, including R. rhodochrous, R. equi, R. rubber, Candida rugosa, and Bacillus cereus. The effect of the culture conditions of R. erythropo/is LG12 on the production of 3-hydroxypropionic acid (3HP) from acrylic acid was also examined. To enhance the production of 3HP, acrylic acid-assimilating activity was induced by adding 1 mM acrylic acid to the culture medium when the cell density reached an $OD_{600}$ of 5. Further cultivation of R. erythropo/is LG 12 with 40 g/l of acrylic acid resulted in the production of 17.5 g/l of 3HP with a molar conversion yield of 44% and productivity of 0.22 g/l/h at $30^{\circ}C$ after 72 h.

• 한국융합학회논문지
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• 제8권7호
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• pp.167-173
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• 2017

본 연구는 조리기구와 친환경 식문화콘텐츠의 접목형 융합상품으로 최근 소비자의 니즈를 반영한 단순한 전시용도가 아닌 새로운 상품으로서의 가능성을 제시하였다. 또한, 상품화를 위하여 본 과제에서 제작할 디자인이 반영되도록 3D Rendering, 3D Mock-up 등을 활용해 별도의 제작공정을 들여 프로토타입 형태로 제작하고, 이후 생산자와 협의 등을 통해 다양한 상품으로 확장 고려 될 수 있을 것으로 판단된다. 국내는 물론 국외에도 적용할 수 있는 조리 기구를 개발, 디자인함으로써 각자가 자신의 취향에 맞게 식재료를 가열하거나 고기 등을 구워 먹을 수 있도록 한 제품으로 기존에 출시된 그릴 팬에서는 찾을 수 없는 보다 앞서가는 기능과 현대의 변화된 식문화에 잘 부합하며, 감성적이며 형태적으로 교류할 수 있는 디자인을 더하여 참신하고 혁신적인 주방제품 트렌드를 리드할 수 있도록 하였다.

• Tissue Engineering and Regenerative Medicine
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• 제15권6호
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• pp.781-791
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• 2018

BACKGROUND: Glucosamine hydrochloride (GlcN HCl) has been shown to inhibit cell growth and matrix synthesis, but not with N-acetyl-glucosamine (GlcNAc) supplementation. This effect might be related to an inhibition of critical growth factors (GF), or to a different metabolization of the two glucosamine derivatives. The aim of the present study was to evaluate the synergy between GlcN HCl, GlcNAc, and GF on proliferation and cartilage matrix synthesis. METHOD: Bovine chondrocytes were cultivated in monolayers for 48 h and in three-dimensional (3D) chitosan scaffolds for 30 days in perfusion bioreactors. Serum-free (SF) medium was supplemented with either growth factors (GF) $TGF-{\beta}$ ($5ng\;mL^{-1}$) and IGF-I ($10ng\;mL^{-1}$), GlcN HCl or GlcNAc at 1mM each or both. Six groups were compared according to medium supplementation: (a) SF control; (b) SF + GlcN HCl; (c) SF + GlcNAc; (d) SF + GF; (e) SF + GF + GlcN HCl; and (f) SF + GF + GlcNAc. Cell proliferation, proteoglycan, collagen I (COL1), and collagen II (COL2) synthesis were evaluated. RESULTS: The two glucosamines showed opposite effects in monolayer culture: GlcN HCl significantly reduced proliferation and GlcNAc significantly augmented cellular metabolism. In the 30 days 3D culture, the GlcN HCl added to GF stimulated cell proliferation more than when compared to GF only, but the proteoglycan synthesis was smaller than GF. However, GlcNAc added to GF improved the cell proliferation and proteoglycan synthesis more than when compared to GF and GF/GlcN HCl. The synthesis of COL1 and COL2 was observed in all groups containing GF. CONCLUSION: GlcN HCl and GlcNAc increased cell growth and stimulated COL2 synthesis in long-time 3D culture. However, only GlcNAc added to GF improved proteoglycan synthesis.

• Journal of Microbiology and Biotechnology
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• 제32권4호
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• pp.531-540
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• 2022

Due to the high incidence of malignant melanoma, the establishment of in vitro models that recapitulate the tumor microenvironment is of great biological and clinical importance for tumor treatment and drug research. In this study, 3D printing technology was used to prepare GelMA/PEGDA composite scaffolds that mimic the microenvironment of human malignant melanoma cell (A375) growth and construct in vitro melanoma micro-models. The GelMA/PEGDA hybrid scaffold was tested by the mechanical property, cell live/dead assay, cell proliferation assay, cytoskeleton staining and drug loading assay. The growth of tumor cells in two- and three-dimensional culture systems and the anti-cancer effect of luteolin were evaluated using the live/dead staining method and the Cell Counting Kit-8 (CCK-8) method. The results showed a high aggregation of tumor cells on the 3D scaffold, which was suitable for long-term culture. Cytoskeleton staining and immunofluorescent protein staining were used to evaluate the degree of differentiation of tumor cells under 2D and 3D culture systems. The results indicated that 3D bioprinted scaffolds were more suitable for tumor cell expansion and differentiation, and the tumor cells were more aggressive. In addition, luteolin was time- and dose-dependent on tumor cells, and tumor cells in the 3D culture system were more resistant to the drug.