Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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3D Bioprinted GelMA/PEGDA Hybrid Scaffold for Establishing an In Vitro Model of Melanoma

  • Duan, Jiahui (MicroNano System Research Center, College of Information and Computer and Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology) ;
  • Cao, Yanyan (MicroNano System Research Center, College of Information and Computer and Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology) ;
  • Shen, Zhizhong (MicroNano System Research Center, College of Information and Computer and Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology) ;
  • Cheng, Yongqiang (MicroNano System Research Center, College of Information and Computer and Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology) ;
  • Ma, Zhuwei (MicroNano System Research Center, College of Information and Computer and Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology) ;
  • Wang, Lijing (MicroNano System Research Center, College of Information and Computer and Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology) ;
  • Zhang, Yating (MicroNano System Research Center, College of Information and Computer and Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology) ;
  • An, Yuchuan (MicroNano System Research Center, College of Information and Computer and Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology) ;
  • Sang, Shengbo (MicroNano System Research Center, College of Information and Computer and Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology)
  • Received : 2021.11.02
  • Accepted : 2022.01.11
  • Published : 2022.04.28
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Abstract

Due to the high incidence of malignant melanoma, the establishment of in vitro models that recapitulate the tumor microenvironment is of great biological and clinical importance for tumor treatment and drug research. In this study, 3D printing technology was used to prepare GelMA/PEGDA composite scaffolds that mimic the microenvironment of human malignant melanoma cell (A375) growth and construct in vitro melanoma micro-models. The GelMA/PEGDA hybrid scaffold was tested by the mechanical property, cell live/dead assay, cell proliferation assay, cytoskeleton staining and drug loading assay. The growth of tumor cells in two- and three-dimensional culture systems and the anti-cancer effect of luteolin were evaluated using the live/dead staining method and the Cell Counting Kit-8 (CCK-8) method. The results showed a high aggregation of tumor cells on the 3D scaffold, which was suitable for long-term culture. Cytoskeleton staining and immunofluorescent protein staining were used to evaluate the degree of differentiation of tumor cells under 2D and 3D culture systems. The results indicated that 3D bioprinted scaffolds were more suitable for tumor cell expansion and differentiation, and the tumor cells were more aggressive. In addition, luteolin was time- and dose-dependent on tumor cells, and tumor cells in the 3D culture system were more resistant to the drug.

Keywords

Acknowledgement

This study was supported by the National Natural Science Foundation of China (No. 51975400, 62031022) and Shanxi Provincial Key Medical Scientific Research Project (2020XM06).

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