• Korean Journal of Plant Resources
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• v.16 no.3
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• pp.230-237
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• 2003

This study was conducted to investigate the occurrence and characterization of tobacco mosaic virus(TMV) in Chinese foxglove isolated from the field of the Chonbuk province(Jinan, Jangsu, Jeongeup). TMV was detected in all three regions and confirmed positive reaction by ELISA test. In the host range test, Chenopodium amaranticola, Nicotiana glutinosa, N. tabacum cv. 'Bright yellow', N. tabacum cv. 'KY­57, Datura stramonium were locally infected with the virus. The virus produced mosaic symptom on inoculated leaves of N. tabacum cv. 'Samson'. However, Chenopodium quinoa, Glycine max, Raphanus sativus, Cucumis sativus, Cucurbita moschata, Brassica rape and Lycopersion esculentum did not show any symptoms. TMV particles were revealed as a stiff rod shape by transmission electron microscopic(TEM) and measured as 300 nm in length with 18 nm in diameter. Total RNA was extracted from showing symptom loaves infected with TMV and the reverse transcription­polymerase chain reaction (RT­PCR) obtained 531 bp DNA product of RNA with specific primer used. The capsid protein of TMV­RE showed higher amino acid sequence homology(97.7％) with TMV­To than with TMV­P(72.2％). The capsid protein of TMV­152 showed same amino acid sequence homology with TMV­F. The result of comparison of nucleotides sequence homology between TMV­RE strain and other TMV strain showed 94％ homology with others except TMV­P(67.3％) and TMV ­ C(68.6％).

• Korean Journal of Plant Resources
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• v.25 no.5
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• pp.633-639
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• 2012

This study was performed to evaluate the effect of ethanolic extract from the fruit of Empetrum nigrum var. japonicum (EN) on $CCl_4$-induced hepatotoxicity. Orally provided daily for 7 days were 250-mg/kg or 500-mg/kg EN or vehicle, while $CCl_4$ (40 mg/kg) was intraperitoneally injected the day after the last treatment of EN. Twenty-four hours after injection of $CCl_4$, we measured serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, malondialdehyde (MDA) contents, superoxide dismutase (SOD), and catalase (CAT) activity of the liver. The antioxidant activities were measured with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and inhibitory effect on lipid peroxidation. The EN showed a strong DPPH radical scavenging activity and inhibitory effect of lipid peroxidation. The ALT and AST levels in serum were greatly enhanced by the $CCl_4$ injection. However, in the EN treatment group, the levels of ALT and AST in serum were significantly reduced. Moreover, $CCl_4$ significantly increased the MDA contents and decreased the SOD and CAT activity in liver homogenates. The EN recovered MDA contents, close to that in the normal group, while the EN increased the SOD and CAT activity. These results suggest that ethanolic extract from the fruit of Empetrum nigrum var. japonicum has significant antioxidant activity and hepatic protection potential.

• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.80-88
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• 2012

This study was conducted to clarify the antimicrobial effects and radical scavenging activities of the cosmetic compositions having the natural plant pigments, and to enhance the natural materials utilization of cosmetics. The antimicrobial activities of the fifteen kinds of cosmetic composition having natural plant pigments were evaluated using the agar diffusion method. Most of the cosmetic composition having the natural pigments showed the clear zone formation of growth inhibition against Bacillus subtilis, Staphylococcus aureus, Listeria monocytogenes, Vibrio parahaemolyticus and Aspergillus flavus. Especially, purple sweet potato, bitter melon, mulberry leaf and gromwell showed the higher antimicrobial activities. All the cosmetic compositions were evaluated for their antioxidant activity using DPPH radical scavenging capacity and nitrite scavenging ability activity. Both of the free radical DPPH and nitrite scavenging ability was the highest in the cosmetic compositions of onion peel, and these antioxidant activity was significant differences according to different plant pigments. In this study, we conjectured that the plant pigment had the potent biological activities, therefore these plant resources having functional components could be a good materials for development into source of natural cosmetics.

• Korean Journal of Plant Resources
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• v.28 no.4
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• pp.391-399
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• 2015

There has been little research on the harvesting time-dependent changes in the antioxidant activities of new varieties of highland cultures of Gomchwi (Ligularia fischeri) crossed with Turcs (Ligularia fischeri (Ledeb.)) and Nakai (Ligularia fischeri var. spiciformis), namely Sammany (S), Gommany (G) and Damogy (D). This study was conducted to assess the effect of different harvesting times on nutritional and health-related properties such as total phenolic contents, flavonoids, DPPH (1,1-diphenyl-2-picrylhydrasyl) free radical scavenging activities and reducing power. From these harvests, extracts were prepared using methanol. Total phenolic content in Jul 14-G (Gommany harvested on July 14, 0.172 ㎎·GAE/ml) was higher than that in other products harvested after the same period (S, 0.154; D, 0.141 ㎎·GAE/ml). Flavonoid content was higher in Jul 3-G (0.114 ㎎·QE/ml), compared to Jul 3-S (0.113 ㎎·QE/ml) and Jul 14-D (0.089 ㎎·QE/ml). Antioxidant activities were higher in samples harvested after June 12 in all cases. On July 14, the highest DPPH free radical scavenging activities among all harvest dates were seen (92.875~94.595%). The reducing power was also dependent on harvest day (Abs 0.5~0.6 on July 14), showing a pattern similar to that of DPPH free radical-scavenging activities. Antioxidant activity and harvesting times seem to correlate with total polyphenol and flavonoid contents.

• Journal of Gifted/Talented Education
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• v.25 no.6
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• pp.817-836
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• 2015

This research focuses on the development of gifted child behavior checklist which feasibly has application on the nation-wide gifted children observation-recommendation method. Corresponding measure has significance as it reflects actual observations of teachers teaching gifted children first-hand and involves measure of importance regarding each characteristic. An open survey on gifted children behavior characteristics lists and specific behavior patterns has been acquired from teachers in gifted education, and the checklist was developed through expert group review, pre-test, and confirmatory factor analysis process. The former checklists have shown several difficulties on application of observation-recommendation on the field due to behaviors that can't be observed in school, less important behaviors, and collide and duplicate behaviors etc. With regard to such problems, problematic clauses were removed based on the observation probability and importance of the behaviors. Ultimately, total of 32 behavior characteristic checklist consisting of ten sub factors(logical thinking, high achievement, originality, perfectionism, creative problem solving, curiosity, task commitment, conversation ability, creativity, passion) and two to three questions on each factor had been drawn. Through internal consistency test and item-total score correlation, each item of the measure has been analyzed to be consistently evaluating corresponding variables. In addition, the result of confirmatory factor analysis showed every item to be weighed appropriately on its sub-factor, strongly suggesting its feasibility on observation-recommendation of elementary gifted children as an appropriate checklist.

• Journal of Gifted/Talented Education
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• v.19 no.2
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• pp.211-240
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• 2009

This paper attempts to explore issues related with the Sifted and talented education in legal aspects. To accomplish this goal, the legal system connected with the gifted and talented education is examined. It includes the Constitution of the Republic of Korea, framework act on education, elementary and secondary education act, etc,. Second, the institutional process of the Act on the Promotion of Education for the gifted and Talented Law and legal characteristics is reviewed. It is found that the Act on the Promotion of Education for the gifted and Talented Law and its regulations is designated to achieve goals of education in general. Educational institutions for children with gifted and talented seem to attain more investment than general education institutions. Third, main issues in the Act on the Promotion of Education for the gifted and Talented Law is discussed. They are the legal system, legal name and aim, rights and obligation for gifted and talented, selection of gifted and talented, organization and operation of curriculum, and teacher education. In conclusion, it needs deeper study on each issue and needs to be presented the specific alternatives. So, it should be improved the law in such a way to meet the fundamental human rights for the gifted and talented.

• Journal of Gifted/Talented Education
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• v.19 no.2
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• pp.279-302
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• 2009

This paper was to examine the effects of university-based science gifted education program on scientifically gifted students' science problem finding ability and cognitive-affective factors. For this study, 69 scientifically gifted students with previous Silted education experiences at the university-based science gifted education centers and 91 scientifically gifted students without any previous gifted education experiences were compared. Both groups of students were currently enrolled at a specialized science high school. To compare both groups, scientific problem finding ability, science anxiety, science related attitude, self-efficacy, intrinsic motivation, test anxiety, cognitive strategies, and self-regulation were measured. The results indicate that there was no significant difference on scientific problem finding ability between these two groups. The comparison between the past and now in the aspects of variables observed in the study showed that both groups of students were decreased in the science anxiety and self-efficacy. But the test anxiety was significantly increased compared to the past in both groups. Implications of the study were discussed in depth.

• Korean Journal of Food Science and Technology
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• v.37 no.4
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• pp.611-617
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• 2005

Dispersed repetitive DNA elements in genomes of microorganisms differ among and within species. Because distances between repetitive sequences vary depending on bacterial strains, genomic fingerprinting with interspersed repetitive sequence-based probes can be used to distinguish unrelated organisms. Among well-known bacterial repetitive sequences, Repetitive Extragenic Palindromic (REP) sequence has been used to identify environmental bacterial species and strains. We applied REP-PCR to detect and differentiate four major Gram-negative food-borne bacterial pathogens, E. coli, Salmonella, Shigella, and Vibrio. Target DNA fragments of these pathogens were amplified by REP-PCR method. PCR-generated DNA fragments were separated on 1.5% agarose gel. Dendrograms for PCR products of each strain were constructed using photo-documentation system. REP-PCR reactions with primer pairs REP1R-I and REP2-I revealed distinct REP-PCR-derived genomic fingerprinting patterns from E. coli, Salmonella, Shigella, and Vibrio. REP-PCR method provided clear distinctions among different bacterial species containing REP-repetitive elements and can be widely used for typing food-borne Gram-negative strains. Results showed established REP-PCR reaction conditions and generated dendrograms could be used with other supplementary genotyping or phenotyping methods to identify isolates from outbreak and to estimate relative degrees of genetic similarities among isolates from different outbreaks to determine whether they are clonally related.

• Korean Journal of Food Science and Technology
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• v.37 no.4
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• pp.513-518
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• 2005

Capillary electrophoresis (CE) method was developed to determine (+)-catechin and (-)-epicatechin contents in grape seed ethanol extract. CE separation was achieved using 100 mM phosphate and borate buffer at pH 6.0 as background electrolyte and fused silica capillary with 50 microns x 375 microns O.D. (effective length 20.0cm) maintained at $25^{\circ}C$. The applied voltage was 10kV, and detection was performed by DAD at 210 nm, Two catechins were well separated within 6 min with repeatability of <0.8% RSD for migration time and <2.0% RSD for peak area, and correlation coefficients higher than 0.994 were obtained from 58.0 to 174.0 mg/L with detection limit of 0.035 mg/L. Separated compounds were successfully determined. CE method was easy to handle and showed good reproducibility. CE method was compared with conventional coloring and HPLC methods, and main advantages of CE method were low amount of sample required, simple pre-sample treatment, good recovery rate, and short analysis time.

• Korean Journal of Food Science and Technology
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• v.32 no.1
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• pp.111-116
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• 2000

Effects of ultra-high pressure treatment on viable cell count and quality characteristics of low salt fermented anchovy under different operating conditions such as pressure$(200{\sim}500\;MPa)$, temperature$(20{\sim}50^{\circ}C)$ and treatment time$(5{\sim}20\;min)$ were investigated. Viable cell count decreased gradually with the increase of pressure and suddenly at 400 MPa. It also decreased by seven folds at $50^{\circ}C$ and logarithmically with the increase of treatment time. Peroxide value increased with the increase of pressure, temperature and treatment time, and temperature played a major role. Thiobarbituric acid value was higher by two folds in samples treated than in the untreated regardless of any conditions investigated. Volatile basic nitrogen was almost the same in all samples except the one at $50^{\circ}C$. The sample treated at greater than $30^{\circ}C$ under high hydrostatic pressure indicated higher value in amino nitrogen. Treatment at $20^{\circ}C/300$ MPa/15 min showed greater reductions in viable cell counts, remaining better quality of low salt fermented anchovy.