• Restorative Dentistry and Endodontics
• /
• v.34 no.4
• /
• pp.350-355
• /
• 2009

Bond strength depends on characteristics of bonding surface and restorative technique. The majority of studies dealing with dentin bond strength were carried out on flat bonding surface, therefore, difference of bond strength between axial wall and pulpal wall is not clear yet. This study evaluated bonding difference between cavity walls in class I composite resin restoration with different filling techniques. Twenty extracted caries-free human third molars were used. Cavities were prepared in 6 ${\times}$4 ${\times}$3 mm box-type and divided into four groups according to filling technique and bonding surface: Group I; bulk filling - pulpal wall, Group II; bulk filling - axial wall, Group III; incremental filling - pulpal wall, Group IV; incremental filling - axial wall. Cavities were filled with Filtek $Z250^{(R)}$(3M/ESPE., USA) and Clearfill SE $bond^{(R)}$(Kuraray, Japan). After 24 hour-storage in $37^{\circ}C$water, the resin bonded teeth were sectioned bucco-lingualy at the center of cavity. Specimens were vertically sectioned into 1.0 ${\times}$1.0 mm thick serial sticks perpendicular to the bond surface using a low-speed diamond saw (Accutom 50, Struers, Copenhagen, Denmark) under water cooling. The trimmed specimens were then attached to the testing device and in turn, was placed in a universal testing machine (EZ test, Shimadzu Co., Kyoto, Japan) for micro-tensile testing at a cross-head speed of 1 mm/min. The results obtained were statistically analyzed using 2-way ANOVA and t-test at a significance level of 95%. The results were as follows: 1. There was no significant difference between bulk filling and incremental filling. 2. There was no significant difference between pulpal wall and axial wall, either. Within the limit of this study, it was concluded that microtensile bond strength was not affected by the filling technique and the site of cavity walls.

• Journal of the Korean Applied Science and Technology
• /
• v.36 no.3
• /
• pp.758-765
• /
• 2019

The purpose of the this study was to determine the comparative analysis of body muscle activities in plank exercise with and without thera-band. Twelve healthy adult males(age, $21.75{\pm}.57$ years; height, $173.33{\pm}1.34cm$; body mass, $65.92{\pm}1.64kg$; and BMI, $21.93{\pm}.46kg/m^2$) participated in this study as subjects. Plank exercises(full, elbow, side, and reverse plank) were performed with four different thera-band in without(WT), red color(RT), blue color(BT), and siver color(ST). We measured the muscle activities of the erector spinae(ES), deltoideus p. acromialis(DA), external oblique(EO), rectus abdominis(RA), rectus femoris(RF), latissimus dorsi(LD), pectoralis major(PM), and biceps femoris(BF). The research findings were as follows. ES and DA muscle activities were greatest during full plank performed with the WT(p<.05). EO, RA, RF, and PM muscle activities were greatest during full plank performed with the ST(p<.05). ES and DA muscle activities were greatest during elbow plank performed with the WT(p<.05). RF and PM muscle activities were greatest during elbow plank performed with the ST(p<.05). ES, EO, RA, RF, LD, PM, and BF muscle activities were greatest during side plank performed with the ST(p<.05). DA, EO, RA, RF, LD, PM, and BF muscle activities were greatest during reverse plank performed with the ST(p<.05). These results are expected to serve as reference materials for plank exercise applications in training programs for body muscle strengthening.

• Journal of Plant Biotechnology
• /
• v.46 no.3
• /
• pp.189-204
• /
• 2019

In this study, we analyzed the changes in glucosinolate content and gene expression in TO1000DH3 and Early big seedling upon methyl jasmonate (MeJA) treatment. Analysis of glucosinolate contents after MeJA treatment at $200{\mu}M$ concentration showed that the total glucosinolate content increased by 1.3-1.5 fold in TO1000DH3 and 1.3-3.8 fold in Early big compared to those before treatment. Aliphatic glucosinolates, progoitrin and gluconapin, were detected only in TO1000DH3, and the changes in the content of neoglucobrassicin were the greatest at 48 hours after MeJA treatment in TO1000DH3 and Early big. The transcriptomic analysis showed that transcripts involved in stress or defense reactions, or those related to growth were specifically expressed in TO1000DH3, while transcripts related to nucleosides or ATP biosynthesis were specifically expressed in Early big. GO analysis on transcripts with more than two-fold change in expression upon MeJA treatment, corresponding to 12,020 transcripts in TO1000DH3 and 13,510 transcripts in Early big, showed that the expression of transcripts that react to stimulus and chemical increased in TO1000DH3 and Early big, while those related to single-organism and ribosome synthesis decreased. In particular, the expression increased for all transcripts related to indole glucosinolate biosynthesis, which is associated with increase in glucobrassicin and neoglucobrassicin contents. Upon MeJA treatment, the expression of AOP3 (Bo9g006220, Bo9g006240), TGG1 (Bo14804s010) increased only in TO1000DH3, while the expression of Dof1.1 (Bo5g008360), UGT74C1 (Bo4g177540), and GSL-OH (Bo4g173560, Bo4g173550, Bo4g173530) increased specifically in Early big.

• Journal of Nutrition and Health
• /
• v.52 no.5
• /
• pp.413-421
• /
• 2019

Purpose: Astragalus membranaceus (AM) is an important traditional medicinal herb. Pharmacological research has indicated that AM has various physiological activities such as antioxidant, anti-inflammatory, immunoregulatory, anticancer, hypolipidemic, antihyperglycemic, and hepatoprotective activities. The bioactive substances responsible for the physiological activities in AM, including many antioxidant substances, change during the roasting process. This study investigated and compared the changes in the antioxidant constituents of AM caused by roasting. Methods: DPPH (1,1-diphenyl-2-picryl hydrazyl) and $ABTS^+$ (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) radical scavenging activities and their total phenolic content (TPC) were measured. High-performance liquid chromatography (HPLC) analysis was performed to confirm any changes in the isoflavonoids of roasted AM (R-AM),. The cell viability of UVB-induced HDF (Human dermal fibroblast) cells treated with AM and R-AM extracts was investigated. The comet assay was used to examine the inhibitory effects of R-AM extracts on DNA damage caused by oxidative stress. Results: The DPPH and $ABTS^+$ radical scavenging activities were $564.6{\pm}20.9$ and $108.2{\pm}3.1$ ($IC_{50}$ value) respectively, from the 2R-AM. The total phenol content was $47.80{\pm}1.40mg$ GAE/g from the 1R-AM. The values of calycosin and formononetin, which are the known isoflavonoid constituents of AM, were $778.58{\pm}2.72$ and $726.80{\pm}3.45{\mu}g/g$ respectively, from the 2R-AM. Treatment of the HDF cells with R-AM ($50{\sim}200{\mu}g/mL$) did not affect the cell viability. Furthermore, the R-AM extracts effectively protected against UVB-induced DNA damage. Conclusion: The findings of this study indicate that R-AM increases its isoflavonoid constituents and protects against UVB-induced DNA damage in HDF cells.

• Korean journal of applied entomology
• /
• v.58 no.2
• /
• pp.151-157
• /
• 2019

It was available to store both nymphs and adults of Geocoris pallidipennis at $5^{\circ}C$ for 3 weeks without negative effects on their survival, fecundity, and Bemisia tabaci predation. Fecundities of G. pallidipennis showed the tendency to be decreased with increasing cold storage duration, but up to 4 weeks, storage at $5^{\circ}C$ didn't affect their fecundity. When Micromus angulatus adults were stored at $5^{\circ}C$ with honey solution as preys, it was possible to store up to 140 days showing approximately 50% of cumulative survival, but over 2 week cold storage was not suitable for their fecundity. With the increase of cold storage duration, the aphid predation by M. angulatus decreased, but it could be possible to cold store until 5 weeks without any significant effect on their aphid predation. Thus, short term cold storage of G. pallidipennis and M. angulatus could be useful for inundative biological strategies.

• Journal of Korea Society of Waste Management
• /
• v.34 no.2
• /
• pp.205-213
• /
• 2017

The current study was performed to investigate the effect of recycling coir substrates on the growth, fruit yield, and quality of strawberry plants. Analysis of physical properties revealed that the pH of a fresh coir substrate was 5.04 while those of substrates reused for one and two years were 5.20 and 5.33, respectively. The electrical conductivity (EC) of a new substrate was as high as $4.58dS{\cdot}m^{-1}$. This can cause salt stress after transplanting. The EC tended to decrease as the substrate was recycled, and the EC of a two-year recycled substrate was $1.48dS{\cdot}m^{-1}$. The fresh substrate had lower nitrogen and calcium concentrations, but higher phosphate, potassium, and sodium concentrations than the recycled coir substrate. The coir substrates recycled for one or two years maintained better chemical properties for plant growth than the fresh substrate. Strawberry growth varied depending on the number of years that the coir substrate was recycled. In general, strawberries grown in substrates that had been reused for two years did better than those grown in substrates that had been reused once or were fresh. Ninety days after transplanting, a plant grown in a substrate that had been reused for two years contained 25 leaves, which was 3.6 more than with a fresh substrate. In addition, the plants grown in a substrate that had been reused for two years exhibited larger leaf areas than those grown in other substrates. Coir substrates that had been reused for one year increased the number and area of leaves, but not as much as the substrate that had been reused for two years. One- and two-year reused coir substrates increased the weight of strawberries produced relative to the unused substrate, but the difference was not statistically significant. The plants grown in two-year reused substrates were longer and wider, as well. Also, the number of fruits per plant was higher when substrates were reused. Specifically, the number of fruits per plant was 28.7 with a two-year reused substrate, but only 22.2 with a fresh substrate. The fruit color indices (as represented by their Hunter L, a, b values) were not considerably affected by recycling of the coir substrate. The Hunter L value, which indicates the brightness of the fruit, did not change significantly when the substrate was recycled. Neither Hunter a (red) nor b (yellow) values were changed by recycling. In addition, there were no significant changes in the hardnesses, acidities, or soluble solid-acid ratios of fruits grown in recycled substrates. Thus, it is thought that recycling the coir substrate does not affect measures of fruit quality such as color, hardness, and sugar content. Overall, reuse of coir substrates from hydroponic culture as high-bed strawberry growth substrates would solve the problems of new substrate costs and the disposal of substrates that had been used once.

• The Korean Journal of Mycology
• /
• v.49 no.3
• /
• pp.337-349
• /
• 2021

This study aimed to isolate wild yeasts from water and soil sample of the Nonsan stream and Sapgyoho (lake) in Chungcheonnam-do, Korea, and to further characterize previously unrecorded wild yeast strains. In total, 102 strains, representing 55 different species of wild yeast were isolated from 95 samples collected from the Jangseoncheon and Ipchoncheon of Nonsan stream in Jellabuk-do and Chungcheonnam-do. Among these, 33 strains were isolated from alkalophilic yeast extract-peptone-dextrose (YPD) medium (pH 9.0), and 9 strains were isolated concurrently on general YPD medium (pH 6.5) and alkalophilic medium. Seventeen strains of Cryptococcus laurentii were predominantly isolated. Additionally, 65 strains, representing 27 different species of wild yeast were isolated from 58 samples obtained from Sapgyoho (lake). Among the 82 isolated wild yeast strains, 8 strains, including Candida fructus JSC 72-1(JSL-GGU 015), had not previously been recorded. All 8 previously unrecorded yeasts were oval in shape except C. fructus JSC72-1(JSL-GGU-015), and only the Filobasidium chernovii JSC39-1(JSL-GGU-013) strain formed spores. All strains except Pseudosydowia eucalypti JSC23-6(JSL-GGU-012) grew well in yeast extract-peptone-dextrose (YPD) and yeast extract-malt extract media and grew in vitamin-free medium. Four strains, including P.eucalypti JSC23-6(JSL-GGU-012) grew well in 15% NaCl-containing YPD medium. F.chernovii JSC39-1(JSL-GGU-013) and Sirobasidium intermedium JSC7-3(JSL-GGU-014) assimilated lactose, and five strains, including F. chernovii JSC39-1(JSL-GGU-013) also assimilated starch. All strains were resistant to 800 ppm of Ca, Cu, Li, and Mg ions.

• Korean Journal of Ecology and Environment
• /
• v.55 no.2
• /
• pp.99-110
• /
• 2022

We investigated physicochemical properties and isotopic compositions of organic matter (δ¹³C_TOC and δ ¹⁵N_TN) in the old fish farming (OFF) site after the cessation of aquaculture farming. Based on this approach, our objective is to determine the organic matter origin and their relative contributions preserved at sediments of fish farming. Temporal and spatial distribution of particulate and sinking organic matter(OFF sites: 2.0 to 3.3 mg L^-1 for particulate matter concentration, 18.8 to 246.6 g m^-2 day^-1 for sinking organic matter rate, control sites: 2.0 to 3.5 mg L^-1 for particulate matter concentration, 25.5 to 129.4 g m^-2 day^-1 for sinking organic matter rate) between both sites showed significant difference along seasonal precipitations. In contrast to variations of δ¹³C_TOC and δ¹⁵N_TN values at water columns, these isotopic compositions (OFF sites: -21.5‰ to -20.4‰ for δ¹³ C_TOC, 6.0‰ to 7.6‰ for δ¹⁵N_TN, control sites: -21.6‰ to -21.0‰ for δ¹³C_TOC, 6.6‰ to 8.0‰ for δ¹⁵N_TN) investigated at sediments have distinctive isotopic patterns(p<0.05) for seawater-derived nitrogen sources, indicating the increased input of aquaculture-derived sources (e.g., fish fecal). With respect to past fish farming activities, representative sources(e.g., fish fecal and algae) between both sites showed significant difference (p<0.05), confirming predominant contribution (55.9±4.6%) of fish fecal within OFF sites. Thus, our results may determine specific controlling factor for sustainable use of fish farming sites by estimating the discriminative contributions of organic matter between both sites.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.11 no.1
• /
• pp.103-111
• /
• 2009

This study was carried out to determine effects of long-term supply of Cordyceps militaris mycelia on cordycepin content in milk in dairy cows. Ten Holstein cows in the early stages of lactation were divided into two groups. Control group received no supplement whereas treatment group received 6% of C. militaris mycelia of their dry matter intake for 6 months. Feed intake, milk urea nitrogen and somatic cell counts were not affected by long-term supply of C. militaris mycelia for the whole period. In addition, milk yield and milk composition were not affected by long-term supply of C. militaris mycelia at any time of the periods with the exception of milk protein content and yield. The average of milk protein content and yield from the whole period was higher for C. militaris mycelia supplement group than for the control group. As expected, cordycepin in whole blood and milk was not detected in the control group. The range of cordycepin content in the treatment was 0.31~0.38µ/ml and 0.18~0.26(µ/ml for whole blood and milk, respectively. Individual variation was found to be very high and, furthermore cordycepin was undetected in some milk samples. Thus, no clear pattern could be seen in cordycepin content in milk throughout the whole period. Overall, the results of the present study suggest that the transfer efficiency of cordycepin to milk by supplementing C. militaris mycelia in dairy cows was unpredictable and low.

• Journal of Marine Life Science
• /
• v.8 no.1
• /
• pp.8-31
• /
• 2023

To understand the distribution and formation mechanism of benthic foraminiferal assemblages, grain size analysis, ¹⁴C radiocarbon dating, and benthic foraminifera analysis were conducted on thirty-two surface sediments collected from the continental shelf of the northern East China Sea, respectively. Surface sediment was composed of sandy mud~muddy sand facies with an average of 52.04% of sand, 13.72% of silt, and 34.20% of clay. These sedimentary facies are palimpsest sediment. Benthic foraminifera was classified into a total of 48 genera and 104 species, including agglutinated foraminifera, calcareous-hyaline, and calcareous-porcelaneous foraminifera. The production rate of agglutinated foraminifera increased toward the Yangtze River area while that of planktonic foraminifera increased toward Jeju Island. Dominant species are Ammonia ketienziensis, Bolivina robusta, Eggella advena, Eilohedra nipponica, Pseudorotalia gamardii, Pseudoparrella naraensis. ¹⁴C radiocarbon datings of Bolivina robusta and Pseudorotalia gamardii with the highest production rate were 2,360±40 yr B.P. and 2,450±40 yr B.P., respectively. In the result of cluster analysis, three assemblages composed of P. gaimardii, B. robusta, and A. ketienziensis-P. naraensis were classified broadly. P. gaimardii assemblage is thought to be formed from about 2.5 yr B.P. at the sea area of the Yangtze River to 50 m in water depth affected by fresh water. B. robusta assemblage is thought to be formed from about 2.4 yr B.P. at the sea area of Jeju Island to 50~100 m affected by offshore water. And then, A. ketienziensisP. naraensis assemblage was formed in the northwest sea area (Central Yellow Sea Mud). These distributions and composition of benthic foraminiferal assemblages formed from about 2.5 yr B.P. in the northern East China Sea are thought to be due to the change of benthic ecology environment that occurred by the sea level increase during the late Holocene.