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http://dx.doi.org/10.4163/jnh.2019.52.5.413

Changes in the constituents and UV-photoprotective activity of Astragalus membranaceus caused by roasting  

Park, Jeong-Yong (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Lee, Ji Yeon (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Kim, Hyung Don (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Jang, Gwi Yeong (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Seo, Kyung Hye (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Publication Information
Journal of Nutrition and Health / v.52, no.5, 2019 , pp. 413-421 More about this Journal
Abstract
Purpose: Astragalus membranaceus (AM) is an important traditional medicinal herb. Pharmacological research has indicated that AM has various physiological activities such as antioxidant, anti-inflammatory, immunoregulatory, anticancer, hypolipidemic, antihyperglycemic, and hepatoprotective activities. The bioactive substances responsible for the physiological activities in AM, including many antioxidant substances, change during the roasting process. This study investigated and compared the changes in the antioxidant constituents of AM caused by roasting. Methods: DPPH (1,1-diphenyl-2-picryl hydrazyl) and $ABTS^+$ (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) radical scavenging activities and their total phenolic content (TPC) were measured. High-performance liquid chromatography (HPLC) analysis was performed to confirm any changes in the isoflavonoids of roasted AM (R-AM),. The cell viability of UVB-induced HDF (Human dermal fibroblast) cells treated with AM and R-AM extracts was investigated. The comet assay was used to examine the inhibitory effects of R-AM extracts on DNA damage caused by oxidative stress. Results: The DPPH and $ABTS^+$ radical scavenging activities were $564.6{\pm}20.9$ and $108.2{\pm}3.1$ ($IC_{50}$ value) respectively, from the 2R-AM. The total phenol content was $47.80{\pm}1.40mg$ GAE/g from the 1R-AM. The values of calycosin and formononetin, which are the known isoflavonoid constituents of AM, were $778.58{\pm}2.72$ and $726.80{\pm}3.45{\mu}g/g$ respectively, from the 2R-AM. Treatment of the HDF cells with R-AM ($50{\sim}200{\mu}g/mL$) did not affect the cell viability. Furthermore, the R-AM extracts effectively protected against UVB-induced DNA damage. Conclusion: The findings of this study indicate that R-AM increases its isoflavonoid constituents and protects against UVB-induced DNA damage in HDF cells.
Keywords
Astragalus membranaceus; UV-photoprotective activity; antioxidant; DNA damage;
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Times Cited By KSCI : 7  (Citation Analysis)
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1 Jiang SJ, Chu AW, Lu ZF, Pan MH, Che DF, Zhou XJ. Ultraviolet B-induced alterations of the skin barrier and epidermal calcium gradient. Exp Dermatol 2007; 16(12): 985-992.   DOI
2 Lee MY, Han JH, Kang MH. Protective effect of Korean diet food groups on lymphocyte DNA damage and contribution of each food group to total dietary antioxidant capacity (TDAC). J Nutr Health 2016; 49(5): 277-287.   DOI
3 Im KR, Kim MJ, Jung TK, Yoon KS. Analysis of isoflavonoid contents in Astragalus membranaceus Bunge cultivated in different areas and at various ages. KSBB J 2010; 25(3): 271-276.
4 Park SY, Lim JM, Choi YH, Choi HS, Kim JH, Kim EJ, et al. Quality and sensory characteristics of soy sauces containing Astragalus membranaceus by aging period. Korean J Food Preserv 2015; 22(5): 636-643.   DOI
5 Lai PK, Chan JY, Cheng L, Lau CP, Han SQ, Leung PC, et al. Isolation of anti-inflammatory fractions and compounds from the root of Astragalus membranaceus. Phytother Res 2013; 27(4): 581-587.   DOI
6 Jia WC, Liu G, Zhang CD, Zhang SP. Formononetin attenuates hydrogen peroxide (H2O2)-induced apoptosis and NF-${\kappa}B$ activation in RGC-5 cells. Eur Rev Med Pharmacol Sci 2014; 18(15): 2191-2197.
7 Cho WC, Leung KN. In vitro and in vivo anti-tumor effects of Astragalus membranaceus. Cancer Lett 2007; 252(1): 43-54.   DOI
8 Kim MJ, Lim KR, Jung TK, Yoon KS. Anti-aging effect of Astradalus membranaceus root extract. J Soc Cosmet Sci Korea 2007; 33(1): 33-40.
9 Zhang J, Xie X, Li C, Fu P. Systematic review of the renal protective effect of Astragalus membranaceus (root) on diabetic nephropathy in animal models. J Ethnopharmacol 2009; 126(2): 189-196.   DOI
10 Lin LZ, He XG, Lindenmaier M, Nolan G, Yang J, Cleary M, et al. Liquid chromatography-electrospray ionization mass spectrometry study of the flavonoids of the roots of Astragalus mongholicus and A. membranaceus. J Chromatogr A 2000; 876(1-2): 87-95.   DOI
11 Wang X, Zhao L. Calycosin ameliorates diabetes-induced cognitive impairments in rats by reducing oxidative stress via the PI3K/Akt/GSK-$3{\beta}$ signaling pathway. Biochem Biophys Res Commun 2016; 473(2): 428-434.   DOI
12 Kim JH, Kim MR, Lee ES, Lee CH. Inhibitory effects of calycosin isolated from the root of Astragalus membranaceus on melanin biosynthesis. Biol Pharm Bull 2009; 32(2): 264-268.   DOI
13 Li W, Sun YN, Yan XT, Yang SY, Kim S, Lee YM, et al. Flavonoids from Astragalus membranaceus and their inhibitory effects on LPS-stimulated pro-inflammatory cytokine production in bone marrow-derived dendritic cells. Arch Pharm Res 2014; 37(2): 186-192.   DOI
14 Yin Y, Heo SI, Jung MJ, Wang MH. Antioxidant and antidiabetic effects of various sections of Astragalus membranaceus. Korean J Pharmacogn 2009; 40(1): 1-5.
15 Hwang IG, Kim HY, Joung EM, Woo KS, Jeong JH, Yu KW, et al. Changes in ginsenosides and antioxidant activity of Korean ginseng (Panax ginseng C.A. Meyer) with heating temperature and pressure. Food Sci Biotechnol 2010; 19(4): 941-949.   DOI
16 Suh CS, Chun JK. Relationships among the roasting conditions, colors and extractable solid content of roasted barley. Korean J Food Sci Technol 1981; 13(4): 334-339.
17 Park MH, Kim KC, Kim JS. Changes in the physicochemical properties of ginseng by roasting. J Ginseng Res 1993; 17(3): 228-231.
18 Saklar S, Ungan S, Katnas S. Microstructural changes in hazelnuts during roasting. Food Res Int 2003; 36(1): 19-23.   DOI
19 Dewanto V, Wu X, Adom KK, Liu RH. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 2002; 50(10): 3010-3014.   DOI
20 Lee MH, Cho JH, Kim BK. Effect of roasting conditions on the antioxidant activities of Cassia tora L. Korean J Food Sci Technol 2013; 45(5): 657-660.   DOI
21 Park JH, Han JS, Choi HK. Effect on quality of pan-fired green tea by 1st pan-firing time. Korean J Med Crop Sci 1999; 7(2): 101-106.
22 Lee YG, Lim SU, Kim JO. Influence of roasting conditions on the flavor quality of sesame seed oil. Agric Chem Biotechnol 1993; 36(6): 407-415.
23 van den Berg R, Haenen GR, van den Berg H, Bast A. Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures. Food Chem 1999; 66(4): 511-517.   DOI
24 Lee SH, Lee YR, Hwang IG, Woo KS, Kim KH, Kim KJ, et al. Antioxidant activities and quality characteristics of germinated rough rice tea according to roasting temperature, time and leaching condition. Korean J Food Sci Technol 2009; 41(4): 386-391.
25 Oh BH. Pathogenesis and prevention of skin cancer. J Korean Med Assoc 2018; 61(11): 644-648.   DOI
26 Kim J, Park SS, Cho N, Kim W, Cho HK. Recent variations of UV irradiance at Seoul 2004 - 2010. Atmosphere 2011; 21(4): 429-438.   DOI
27 Chong Z, Matsuo H, Onoue S, Yamamoto H, Ito H, Katakura Y. Identification of polyphenols that repair the ultraviolet-B-induced DNA damage via SIRT1-dependent XPC/XPA activation. J Funct Foods 2019; 54: 119-127.   DOI
28 Bondet V, Brand-Williams W, Berset C. Kinetics and mechanisms of antioxidant activity using the DPPH.free radical method. Lebenson Wiss Technol 1997; 30(6): 609-615.   DOI
29 Fazio A, Plastina P, Meijerink J, Witkamp RF, Gabriele B. Comparative analyses of seeds of wild fruits of Rubus and Sambucus species from Southern Italy: fatty acid composition of the oil, total phenolic content, antioxidant and antiinflammatory properties of the methanolic extracts. Food Chem 2013; 140(4): 817-824.   DOI
30 Choi EH, Lee DY, Park HS, Shim SM. Changes in the profiling of bioactive components with the roasting process in Lycium chinense leaves and the anti-obesity effect of its bioaccessible fractions. J Sci Food Agric 2019; 99(9): 4482-4492.   DOI
31 Lim JA, Yun BW, Baek SH. Antioxidative Activity and nitrite scavenging ability of methanol extract from Salvia plebeia R. Br. Korean J Med Crop Sci 2007; 15(3): 183-188.
32 Kim HG, Kim GW, Oh H, Yoo SY, Kim YO, Oh MS. Influence of roasting on the antioxidant activity of small black soybean (Glycine max L. Merrill). Lebenson Wiss Technol 2011; 44(4): 992-998.   DOI
33 Seo EJ, Hong ES, Choi MH, Kim KS, Lee SJ. Antioxidant and skin whitening effects of Rhamnus yoshinoi extracts. Korean J Food Sci Technol 2010; 42(6): 750-754.
34 Nho JW, Hwang IG, Joung EM, Kim HY, Chang SJ, Jeong HS. Biological activities of Magnolia denudata Desr. flower extracts. J Korean Soc Food Sci Nutr 2009; 38(11): 1478-1484.   DOI
35 Perron NR, Brumaghim JL. A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochem Biophys 2009; 53(2): 75-100.   DOI
36 Jabbari M, Jabbari A. Antioxidant potential and DPPH radical scavenging kinetics of water-insoluble flavonoid naringenin in aqueous solution of micelles. Colloids Surf A Physicochem Eng Asp 2016; 489: 392-399.   DOI
37 Jang HL, Park SY, Nam JS. The effects of heat treatment on the nutritional composition and antioxidant properties of hempseed (Cannabis sativa L.). J Korean Soc Food Sci Nutr 2018; 47(9): 885-894.   DOI
38 Floegel A, Kim DO, Chung SJ, Koo SI, Chun OK. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Compost Anal 2011; 24(7): 1043-1048.   DOI
39 Lee JY, Park JY, Kim HD, Lee SE, Lee JH, Lee Y, et al. Anti-oxidant and anti-adipocyte differentiation of Aster glehni and Aster yomena. J Nutr Health 2019; 52(3): 250-257.   DOI
40 Jeong SM, Kim SY, Kim DR, Nam KC, Ahn DU, Lee SC. Effect of seed roasting conditions on the antioxidant activity of defatted sesame meal extracts. J Food Sci 2004; 69(5): C377-C381.   DOI
41 Lee KH, Choi HS, Choi YH, Park SY, Song J. Changes in isoflavone content and quality characteristics of Cheonggukjang prepared with Bacillus subtilis HJ18-3 and KACC 15935. Korean J Food Preserv 2014; 21(1): 121-128.   DOI
42 Kim CS, Lee YS, Kim JS, Han YH. High performance liquid chromatographic analysis of isoflavones in soybean foods. Korean J Food Sci Technol 2000; 32(1): 25-30.