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Comparative Analysis of Endophytic Bacterial Communities in the Roots of Rice Grown under Long-term Fertilization Practice using Pyrosequencing Method (파이로시퀀싱을 이용한 비료 장기 연용지의 벼 뿌리 내생세균의 군집 분석)

  • Kim, Byung-Yong;Ahn, Jae-Hyung;Song, Jaekyeong;Kim, Myung-Sook;Weon, Hang-Yeon
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.6
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    • pp.1100-1107
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    • 2012
  • Bacterial endophytes may be important factors in plant growth and ecologically relevant functions in rice. Using pyrosequencing technology, we analyzed the composition of endophytic bacterial communities that colonized the roots of rice cultivated in long-term fertilized (APK) and non-fertilized (NF) paddy soils. A total of 1,900 reads were obtained from 2 samples. All sequences were classified into 177 OTUs (APK sample) or 72 OTUs (NF sample) at a 97% similarity cut-off. Twenty-two OTUs were shared between the 2 samples, and these were also the most dominant OTUs in both samples. Proteobacteria was the most dominant phylum with 90.2%, followed by Actinobacteria (7.1%) and Bacteroidetes (1.1%). Furthermore, Pseudomonas was the most abundant genus in both samples. We observed clear differences in the structure of the endophytic bacterial community structure between the 2 samples. Notably, the distributions of Alphaproteobacteria and Gammaproteobacteria were markedly different. The diversity index of the APK sample was higher than that of the NF sample. These findings showed that the endophytic bacterial community of rice roots was affected by the presence of fertilizers in the rice field soil.

Effect of Microbial Phytase in Low Phosphorus and Calcium Level Diet on the Performance and Nutrient Digestibility in Laying Hens (인과 칼슘의 수준이 낮은 산란계 사료 내 미생물 Phytase의 첨가가 생산성 및 영양소 소화율에 미치는 영향)

  • Min B.J.;Kwon O.S.;Lee W.B.;Son K.S.;Hong J.W.;Yang S.J.;Moon T.H.;Kim I.H.
    • Korean Journal of Poultry Science
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    • v.32 no.1
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    • pp.15-21
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    • 2005
  • This Study was conducted to investigate the effects of microbial phytase in low phosphirus and calcium level diet on the performance and nutrient digestibility in laying hens. One hundred ninety two, 50 wks old, ISA brown commerical layers were used for 12 weeks feeding trial after 7-d adjustment period. Four dietary treatments included CON(control; Co.), P2 ($0.06\%$ Natuphos, BASF) and P3 ($0.06\%$ PHOSMAX, GENOFOCUS). Ca and available P concentrations of P1, P2 and P3 were 90 and $50\%$ of NRC recommecdations to accentuate difference in response to phytase availability. In whole period, egg production was not affected by treatments. At 12 weeks, egg weight was significantly increased in adding phytase treatments (P<0.05). Egg shell thickness was increased in P1, P2 and P3 treatments compared with control (P<0.05) at 9 weeks. Ca concentration of serum tended to decrease in P1 treatment without significant difference (P>0.05). Ca and P concentrations of tibia were higher in layers fed dietary phyrase than those fed control diet without significant difference (P>0.05). Digestibilities of DM, N and ash were improved in P1 treatment compared with P2 and P3 treatments (P<0.05). Ca and P digestibilities were the highest in P2 treatment (P>0.05), but was not significant difference between control and P1 treatments.

91년 지가동향

  • 한국주택협회
    • 주택과사람들
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    • no.5 s.22
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    • pp.72-85
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    • 1992
  • [ $\bullet$ ] ''91 년간 전국평균지가상승률은 $12.78{\%}$로서 전년대비 $7.8{\%}$포인트 감소. $\bullet$ 서울의 지가상승은 $11.15{\%}$로서 ''90년의 $31.18{\%}$보다 무려 $20.03{\%}$ 포인트 감소하여 전국의 지가안정을 주도하고 있음. ${\cdot}$ 지역별로는 6대도시 : $13.57{\%}$, 중소도시 : $13.69{\%}$, 군지역 : $10.19{\%}$. ${\cdot}$ 용도지역별로는 주거지역 : $13.57{\%}$, 상업지역 : $12.22{\%}$, 공업지역 : $15.96{\%}$, 녹지지역 : $13.80{\%}$, 비도시지역 : $9.42{\%}$. $\bullet$ 특이사항으로는 ${\cdot}$ ''91. 4/4분기중 지가가 하락한 지역이 29개 시${\cdot}$${\cdot}$구로서 이중에서 중요한 지역을 보면 서울 중구($-2.92{\%}$), 용산구($-2.02{\%}$), 강남구($-1.94{\%}$), 대구 수성구($-3.11{\%}$), 대전의 유성구($-3.18{\%}$), 충남 태안군($-3.83{\%}$), 전남 순천시($-2.36{\%}$)), 나주군($-2.42{\%}$등이며, ${\cdot}$ 대전($23.25{\%}$) 및 인천($22.81{\%}$) 지역은 년간 $20{\%}$를 상회하고 있으나 이들 지역에서도 ''91 4/4분기에는 크게 둔화되고 있음. $\bullet$ 전반적으로 ''87년 수준의 지가안정세를 보이고 있으며 분기별 상승률은 ''87. 4/4분기 이래 최소치를 시현하고 있음.

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Numerical Method for Eigen Pairs of a Real Valued Symmetric Matrix (실대칭 행력의 고유쌍에 대한 수치해법)

  • Choi, Seong;Cho, Young-Sik;Baek, Cheong-Ho
    • The Transactions of the Korea Information Processing Society
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    • v.5 no.1
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    • pp.97-102
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    • 1998
  • In the most cases of eigen value problems in the social sciences, the object matrix to analyze is real-valued symmetric matrix. And many cases of eigen value problems in this field needs 2-4 eigen pairs according to the magnitude of their absolute values. The methods to obtain eigen pairs by numerical computation using computer, we would face the problem of round off error because matrix computation needs a number of calculations. In this paper, an algorithm which make us to get some needed eigcn pairs according to the magnitude of their absolute values is designed. And in this algorithm, the power method is used to obtain some eigen pairs. This algorithm is expected to be effective by the reduction of the number of calculations.

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A Production Method to GRM Coefficients of Multiple Valued Logic Function (다치논리함수의 GRM상수 생성 방법)

  • 신부식;심재환;김흥수
    • Journal of the Korean Institute of Telematics and Electronics C
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    • v.36C no.5
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    • pp.67-75
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    • 1999
  • This paper presents a production method to GRM coefficients which are consist of $P^n$ polarities to n variables over GF(p). In general production method to GRM coefficients is derived from RM coefficients to polarity 0 using RM expansion and extended to GRM coefficients. The procedure of proposed production method to GRM coefficients is consists of 2 steps. First, obtain the optimal polarity which is contains a minimal operation to single variable and then apply the same process to all generation process of GRM coefficients using cyclic property of the polarity. Proposed method simplify the generation procedure and reduces a number of operators because of the cyclic property of polarity.

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Antilisterial Synergistic Effect of Falcarindiol Isolated from Dystaemia takesimana Kitagawa with Monoglycride (섬바디로부터 분리된 Falcarindiol과 유화제와의 혼합에 따른 Listeria monocytogenes에 대한 항균 상승효과)

  • Oh, Jin-Ah;Shin, Dong-Hwa;Ahn, Young-Sun
    • Korean Journal of Food Science and Technology
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    • v.31 no.3
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    • pp.864-869
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    • 1999
  • Synergistic effects of the falcarindiol mixed with monoglyceride in broth exhibited strong growth inhibition on all test strains. It was confirmed that the falcarindiol mixed with monoglyceride exhibited bactericidal effect in broth against L. monocytogenes ATCC 19111 and ATCC 19114 at the same level.

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수신신호세기와 초음파를 동시에 이용한 위치측정 기법

  • Park, Chan-Sik;Yun, Hui-Hak;Kim, Seung-Beom;Gang, Dong-Yeon;Lee, Hak-Ju;Cha, Eun-Jong;Lee, Sang-Jeong
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • v.2
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    • pp.541-546
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    • 2006
  • Zigbee를 이용한 실내 위치 측정 기법에서는 수신신호세기(RSSI) 측정치를 거리측정치로 활용하여 삼각측량법이나 DB 검색법을 이용하여 위치를 구한다. RSSI 측정치는 Zigbee 통신에서 기본적으로 제공하는 값으로 하드웨어 추가 없이 거리 측정에 사용할 수 있지만 정확도가 떨어진다. 이를 해결하기 위해 초음파 센서를 장착하고 RF와 초음파의 속도차이를 이용하여 정확한 거리를 측정할 수 있다. 이렇게 구해진 거리를 이용하면 정확한 위치를 구할 수 있다. 이를 구현하는 방법으로 본 논문에서는 고정된 위치의 비컨들이 순차적으로 신호를 전송하여 노드의 위치를 구하는 경우 발생하는 비컨의 전송 스케줄, 추가 및 동기 유지의 어려움을 해결할 수 있도록 노드에서 신호를 전송하고 비컨에서 구한 거리를 다시 노드로 Zigbee 통신을 이용하여 전달하여 위치를 구하는 기법을 제시하였다. 추가로 본 논문에서는 비컨 수의 제약으로 위치를 구할 수 없는 경우에 초음파 측정치에 추가하여 RSSI 측정치를 동시에 이용하는 위치측정기법을 제시하고, 실제 정적, 동적 실험을 통하여 그 유용성을 보였다. 3개 이상의 초음파 신호가 수신되는 경우 cm 급의 정확도로 위치를 10Hz 이상으로 구할 수 있었으며, 2개 이하의 초음파 신호가 수신되는 경우에도 RSSI 측정치가 있으면 수십 cm 급의 정확도로 위치를 구할 수 있음을 확인하였다.

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The Effects of Inorganic Soil Amendment on Growth of Creeping Bentgrass(Agrostis palustris) in Golf Course (무기 토양개량제가 골프장 그린의 크리핑 벤트그래스 생육에 미치는 영향)

  • Lee, Jae-Pil
    • Asian Journal of Turfgrass Science
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    • v.26 no.2
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    • pp.110-115
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    • 2012
  • Soil amendments have been used to improve the physical and chemical condition of turf soil, which might optimize turfgrass growth in golf courses. This study was to investigate the effect of inorganic soil amendment (ZC) established in USGA root zone system on growth of creeping bentgrass, cv. 'Penncross' carried out from May to Dec. in 2005 at the nursery on Sinwon Golf Course. To analyze the effects of inorganic soil amendment on pH, specific gravity, infiltration rate, water content, soil hardness, root length, tiller density and dry weight were measured. pH was 6.7-6.8, specific gravity of sand (S) 100% was 1.48 heavier than the other treatments (1.28-1.38). Infiltration rate with ZC 15% + peat moss (P) 5% + S 80% and ZC 10% + S 90% was faster than S 100%. Soil hardness of S 100% was the highest. Root length of creeping bentgrass of P 10% + S 90% (8.6-12.0 cm) was the longest. Tiller density with P 5% + S 95% was more 4-7 ea than other treatments in summer season. In growing season, however, ZC 10% + P 5% + S 85% was more 2-3 ea than others. Dry weight of creeping bentgrass treated by ZC 10% + P 5% + S 85% in summer season and P 5% + S 95% in growing season were heavier than other treatments. It is recommended to combine 5-15% inorganic soil amendment and peat moss 5% with sand in order to sustain soil balance.

Effect of Feeding Yogurt Using Enterococcus faecium KHM-11 on the Growth in Piglet (Enterococcus faecium KHM-11를 이용한 요구르트 급여가 자돈의 성장에 미치는 영향)

  • Bae, Hyoung-Churl;Lee, Jo-Yoon;Nam, Myoung-Soo
    • Food Science of Animal Resources
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    • v.28 no.2
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    • pp.204-210
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    • 2008
  • A total of 70 colonies were isolated from the Korean human milk samples on the BCP plate count agar. These LAB isolates were subcultured in 10% reconstituted skim milk, and two strain thereof were finally selected for their highest acid productions. These strains were identified as Enterococcusfaecium based on 16S rDNA sequencing data, named as Enterococcus faecium KHM-11. Sugar utilization of E. faecium KHM-11 was investigated by API 50CH kit, and 19 different sugars including D-arabinose, L-arabinose, galactose, D-glucose, D-fructose, and D-mannose were utilized. For fermentation profiles, a yogurt inoculated by E. faecium KHM-11 after 15 hour, reached at pH 4.09, titratable acidity at 1.10% and average viable counts $1.30{\times}10^9\;CFU/mL$. Effects of the administration of yogurt 0.5% of piglet diet to piglets were investigated for growth rate, analysis of blood and incidence of diarrhea. 24 heads of piglets were divided into two groups: the experimental and the control of 12 animals each. The average growth rate in the yogurt-fed group was higher for 21.67%, compared with control (p<0.05). There were no differences in the concentrations of blood glucose, cholesterol, albumin and globulin between the two treatments. Incidence of diarrhea was no in pigs fed yogurt as compare to control.

Selection of Appropiate Plant Species of VFS (Vegetative Filter Strip) for Reducing NPS Pollution of Uplands (밭 비점오염저감을 위한 초생대 적정 초종 선정)

  • Choi, Kyung-Sook;Jang, Jeong-Ryeol
    • Journal of Korea Water Resources Association
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    • v.47 no.10
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    • pp.973-983
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    • 2014
  • This study focused on the selection of appropriate plant species of VFS (vegetative fiter strips) and the assessment of VFS effects for reducing NPS (non-point source) pollution from uplands. The experimental field was constructed with 1 control and 6 treated plots in the upland area of $1,500m^2$ with 5% slope which is located in Gunwi-gun, Gyeongbuk province. Six vegetation including Chufa, Common crabgrass, Barnyard grass, Turf grass, Tall fescue, Kenturky bluegrass, were applied to install VFS systems during the study period from June 2011 to Dec. 2012. The results of this study showed that 6.1~77.8% in runoff and 15.6~90.3% in TS, 49.9~96.6% in T-P, and 6.7~91.1% in T-N were reduced from the VFS treated plots. Generally high reduction effects were observed from TS, T-P, T-N, and SS, while BOD, TOC, and $NO_3^-$ showed low reductions. The best vegetation type was Turf grass showing higher reduction effects of NPS pollutions and having relatively easier maintenance efforts compared to other vegetations selected in this study. Based on these results, VFS technique found to be an effective management practice for reducing agricultural NPS pollutions in Korean upland conditions. Further study needs to be performed through various field experiments with long term monitoring in order to develop a design manual of VFS system for practical applications.