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Startup of Microbial Electrolysis Cells with different mixing ratio of Anaerobic Digested Sludge and Buffer solution (혐기성소화 슬러지 비율에 따른 미생물전기분해전지의 식종 특성)

  • Song, Geunwuk;Baek, Yunjeong;Seo, Hwijin;Jang, Hae-Nam;Chung, Jae Woo;Lee, Myoung-Eun;Ahn, Yongtae
    • Journal of the Korea Organic Resources Recycling Association
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    • v.27 no.4
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    • pp.51-59
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    • 2019
  • In this study, the influence of anaerobic digested sludge and 50 mM PBS (phosphate buffer solution) mixing ratio (1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7) on hydrogen production and inoculation period were examined. MECs were operated in fed-batch mode with an applied voltage of 0.9 V. As a result, in the 1:1 mixing ratio reactor, 9.8-20.9 mL of hydrogen was produced with the highest hydrogen content of 66.8-79.6%. Hydrogen gas production and power density increased from after 12 days of inoculation for the 1:1 mixing ratio reactor. In case of 1:2, 1:3 and 1:4 mixing ratio reactor, the hydrogen gas production was 3.7-7.1 mL and the hydrogen gas content was 5.8-65.8%. The hydrogen gas yield in 1:5, 1:6 and 1:7 ratio reactors, was 0.50-0.69 mL and hydrogen content range was 1.8-7.1%. The mixing ratio was found to be suitable for hydrogen production and inoculation period by mixing ratio up to 1:4.

Transgenic lettuce (Lactuca sativa L.) with increased vitamin C levels using GalUR gene (GalUR 유전자를 이용한 비타민 C 증대 상추 (Lactuca sativa L.) 형질전환체 개발)

  • Lim, Mi-Young;Cho, Yi-Nam;Chae, Won-Ki;Park, Young-Soo;Min, Byung-Whan;Harn, Chee-Hark
    • Journal of Plant Biotechnology
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    • v.35 no.2
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    • pp.115-120
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    • 2008
  • L-Ascorbic acid (vitamin C) in vegetables is an essential component of human nutrition. The objective is to transform lettuce (Lactuca sativa L.) with GalUR gene that is involved in the vitamin C biosynthesis. The cotyledons of Hwoahong (Nongwoo Bio Co.) were used to induce the callus and shoot under the selection media with MS + 30 g/L Sucrose + 0.5 mg/L BAP + 0.1 mg/L NAA + 100 mg/L kanamycin + 200 mg/L lilacillin, pH 5.2. The shoot was developed from the cut side of the explants after 3 weeks on the selection media. We successfully transformed the lettuce with GaIUR gene and analyzed the levels of vitamin C. We found that some of the lettuce transgenic lines contained higher levels of vitamin C compared with the normal one (non-transformed). Especially, some of $T_1$ lettuces inserted by GalUR showed about $3{\sim}4$ times higher content of vitamin C compared to the non-transformed lettuce. This data support the previously work performed with GLOase transgenic $T_1$ lettuces from which several times higher content of vitamin C were identified. The $T_2$ lettuces with high content of vitamin C have been selected for further analysis.

Dechlorination of Atrazine in Sediment Using Zero Valent Iron (영가철($Fe^0$)을 이용한 퇴적물내 Atrazine의 탈염소화)

  • Kim, Geon-Ha;Jeong, Woo-Hyeok;Choe, Seung-Hee
    • Journal of Soil and Groundwater Environment
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    • v.11 no.4
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    • pp.33-40
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    • 2006
  • Residual pesticides discharged from diffuse sources at agricultural area in association with suspended solid will be settled at downstream, and may degrade surface water quality. This research studied dechlorination kinetic of atrazine, one of triazine-category herbicide, using zero-valent iron (ZVI) in sediment. It can be observed from the experiments that buffer capacity of sediment helped pH maintained beutral, resulted in continuous dechlorination. Sediments were spiked with atrazine at 10, 30, and 50 mg atrazine/L of total sediment for batch experiments. Dechlorination constants were $1.38x10^{-1}/d$ for the initial concentration of 10 mg/L, $1.29x10^{-l}/d$ for 30 mg/L, and $7.43x10^{-2}/d$ for 50 mg/L while dechlorination constants of initial concentration of 50 mg/L without ZVI adding were estimated as $3.05x10^{-2}/d. Half lifes atrazine by ZVI were estimated as 5.03 d fur 10 mg/L, 5.38 d for 30 mg/L, and 9.33 d for 50 mg/L, respectively.

Analysis for explosives in contaminated soil using the electrochemical method (폭발물 오염토양에서 전기화학법을 이용한 RDX 흔적량의 분석)

  • Ly, Suw Young
    • Analytical Science and Technology
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    • v.21 no.2
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    • pp.129-134
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    • 2008
  • Cyclic voltammetry (CV) and square wave stripping voltammetry (SW) analysis of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) using the double-stranded ds calf thymus (DNA) mixed in carbon nanotube paste electrode (PE) were provided. The optimum analytical conditions were determined and the peak potential was 0.2 V vs. Ag/AgCl. The linear working ranges of CV (50-75 ug/L) and SW (5-80 ng/L) were obtained. The precisions of RSD in the 10 ug/L was 0.086% (n=15) and the detection limit was 0.65 ng/L ($2.92{\times}10^{-12}M$) (S/N=3) with 300 s adsorption time at the optimum condition. The method was used to determine the presence of explosive chemicals in contaminated soil samples.

Chemical Characteristics of Golf Course Soils in Kyonggi Province (경기도 골프장의 코스별 토양의 화학적 특성)

  • 이인숙
    • Asian Journal of Turfgrass Science
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    • v.8 no.1
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    • pp.25-28
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    • 1994
  • Chemical characteristics of soils were investigated in three golf courses of Kwanak, Gold and Korea Country Club with different open year. The soil samples were collected in tee, fairway and rough. There were ranges of 4.80~5.55 in pH, 3.68~5.39% inorganic matter, 0.10~0.25% in total nitrogen, 5.63~45.64ppm in available phosphorus, 0.80~1.7lme /l00g in exchangeable Ca, 0.09~0 42me /l00g in exchangeable Mg, 0.03~0.O9me /l00g in exchangeable Na, and 0.06~0.l4me /l00g in exchangeable K. The pH was significant with golf club(P<0.05) and positively correlated with exchangeable Ca and exchangeable Mg(P<0.0l).

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Study on the Effects of the Phosphate and Potassium Fertilizer Amount on the Mulberry Yields (숙전에서의 인산및 가리시비량이 뽕잎수량에 미치는 영향)

  • 정태암;박광준;이원주
    • Journal of Sericultural and Entomological Science
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    • v.20 no.2
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    • pp.1-5
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    • 1978
  • The effect of phosphorus and potassium on the leaf yielding from the mulberry field was surveyed, designing four application levels of potassium and phosphorus respectively; Po, P$^1$/$_2$ and P$_1$and P$_2$ for phosphorus, K$^1$/$_3$, K$^2$/$_3$, K$_1$and K$_2$for potassium after amount of nitrogen was constant at 25kg/l0a. The standard application of three elements is at 25-11-15kg/l0a. No application only showed significance among the treatments in the 3rd year autumn. However there is no significance, ever decreasing 2.7∼5.9% of leaf yielding in application of potassium and 6.4∼11.5% of leaf yielding in application of phosphorus. There is also no increase of leaf yielding even with application of double quantity of phosphorus and potassium. Potassium in soil was considerably fluctuated, while fluctuation of phosphorus is very low. With these results, it was evident that the conventional application level for phosphorus (11kg/l0a) is reasonable and for potassium may be cut down to 5∼10kg/l0a without showing any significance for the successive three years.

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Induction and in vitro proliferation of adventitious roots in Dendropanax morbifera (황칠나무(Dendropanax morbifera)의 부정근 유도 및 기내증식조건)

  • Bae, Kee-Hwa;Kim, Ji-Ah;Choi, Yong-Eui
    • Journal of Plant Biotechnology
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    • v.36 no.2
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    • pp.163-169
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    • 2009
  • Dendropanax morbifera (Araliaceae) is an endemic species in Korea and distributed in the southern part of Korea. The roots and stems of this plant have been used for folk medicine for the treatment of migraine headache, dysmenorrheal, and remove wind dampness and for Vanishes production. Production of adventitious roots in D. morbifera by in vitro cultures could be used as alternatives materials. Leaf, stem, and root segments from D. morbifera seedling were cultured on Murashige and Skoog (MS) medium supplemented with 3.0 mg/L IBA and 30 g/L sucrose. After 4 weeks of culture, the highest induction of adventitious roots was obtained from the leaf segment. Frequency of adventitious root formation on medium with various kinds of auxins (IAA, NAA, 2,4-D, and IBA) and various concentrations of IBA (0, 0.1, 0.5, 1.0, 3.0, and 5.0 mg/L) was tested. The maximum induction of adventitious root was obtained on medium with 1.0 mg/L IBA. In liquid culture, growth of root was best 1/2MS medium supplemented with 1.0 mg/L IBA and 30 g/L sucrose. Adventitious roots were cultured in 5 L bioreactor containing 1/2 MS medium supplemented with 1.0 mg/L IBA and 30 g/L sucrose and mass-production of adventitious roots was successfully achieved. This study demonstrated for the first time to produce adventitious roots in D. morbifera.

Decolorization System of Effluent of Activated Sludge Process Using Ozone (오존에 의한 활성오니처리 방류수의 탈색처리 연구)

  • 최희철;곽정훈;최동윤;권두중;김형호;이덕수;강희설;최영수;천상석
    • Journal of Animal Environmental Science
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    • v.8 no.3
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    • pp.153-158
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    • 2002
  • Decolorization system using ozone was developed to reduce the pollutants and dark brown color remained in effluent of activated sludge process for pig wastewater. The results are as follows. 1. Wastewater temperature was increased during the ozone treatment from 31$^{\circ}C$ to 41.9$^{\circ}C$. 2. Oxidation reduction potential(ORP) at the beginning time was 148㎷, but it was increased to 330㎷ according to the ozone treatment. 3. 11mg/l of BO $D_{5}$ in effluent of activated sludge process was decreased to 1mg/l by ozone treatment, CO $D_{MN}$ also decreased from 83mg/l to 1.0mg/l. 4. 442 unit(dark brown color) of color in effluent of activated sludge process was changed to 6 units(colorless), and color removal efficiency was 98.6%.

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Design of SDR-based Multi-Constellation Multi-Frequency GNSS Signal Acquisition/Tracking Module

  • Yoo, Won Jae;Kim, Lawoo;Lee, Yu Dam;Lee, Taek Geun;Lee, Hyung Keun
    • Journal of Positioning, Navigation, and Timing
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    • v.10 no.1
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    • pp.1-12
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    • 2021
  • Due to the Global Navigation Satellite System (GNSS) modernization, the recently launched GNSS satellites transmit signals at various frequency bands of L1, L2 and L5. Considering the Korea Positioning System (KPS) signal and other GNSS augmentation signals in the future, there is a high probability of applying more complex communication techniques to the new GNSS signals. For the reason, GNSS receivers based on flexible Software Defined Radio (SDR) concept needs to be developed to evaluate various experimental communication techniques by accessing each signal processing module in detail. In this paper, we introduce a multi-constellation (GPS/Galileo/BeiDou) multi-band (L1/L2/L5) SDR by utilizing Ettus USRP N210. The signal reception module of the developed SDR includes down-conversion, analog-to-digital conversion, signal acquisition, and tracking. The down-conversion module is designed based on the super-heterodyne method fitted for MHz sampling. The signal acquisition module performs PRN code generation and FFT operation and the signal tracking module implements delay/phase/frequency locked loops only by software. In general, it is difficult to sample entire main lobe components of L5 band signals due to their higher chipping rate compared with L1 and L2 band signals. Experiment result shows that it is possible to acquire and track the under-sampled signals by the developed SDR.

Physiological Responses of Olive Flounder (Paralichthys olivaceus) by Capacity Density Difference during Salinity Change from Seawater to Freshwater (사육수의 담수화시 수용밀도에 따른 넙치(Paralichthys olivaceus)의 생리적 반응 비교)

  • Hur Jun Wook;Lee Bok Kyu;Min Byung Hwa;Park In-Seok;Choi Cheol Young;Lee Jeong Yeol;Chang Young Jin
    • Korean Journal of Environmental Biology
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    • v.22 no.3
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    • pp.419-425
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    • 2004
  • Two different groups (30 and 60 inds.) of olive flounder (Paralichthys olivaceus) were abruptly (within 30 min) exposed to hypo-salinities from seawater (SW, 35$\textperthousand$) to freshwater (FW, 0$\textperthousand$) (30FW and 60FW) and to 35$\textperthousand$ (30SW and 60SW) in a flow through seawater culture systems. Analysis of plasma samples showed the following significant increase at 0$\textperthousand$: cortisol from $2.8\;ng\;mL^{-1}$ to $66.9\;ng\;mL^{-1}$ (30FW) and from $2.7\;ng\;mL^{-1}$ to $314.1\;ng\;mL^{-1}$ (60FW) after 24 hours of exposure; glucose from $15.8\;mg\;dL^{-1}$ to $257.7\;mg\;dL^{-1}$ after 3 hours exposure and to $164.0\;mg\;dL^{-1}$ after 24 hours in 60FW. Plasma $Na^+$ concentration of 30FW and 60FW were decreased until 24 hours after expose. However that in 30SW and 60SW showed no significant differences. Plasma $Cl^-$ concentration of 60FW was decreased from $59.0\;mEq\;L^{-1}$ to $43.5\;mEq\;L^{-1}$ and to $30.0\;mEq\;L^{-1}$ after 3 and 24 hours of exposure, respectively. At all experimental groups, survival were 100% until 24 hours.