Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Induction and in vitro proliferation of adventitious roots in Dendropanax morbifera

황칠나무(Dendropanax morbifera)의 부정근 유도 및 기내증식조건

  • Bae, Kee-Hwa (Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Kim, Ji-Ah (Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Choi, Yong-Eui (Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University)
  • 배기화 (강원대학교 산림자원조성학부) ;
  • 김지아 (강원대학교 산림자원조성학부) ;
  • 최용의 (강원대학교 산림자원조성학부)
  • Published : 2009.06.30
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Abstract

Dendropanax morbifera (Araliaceae) is an endemic species in Korea and distributed in the southern part of Korea. The roots and stems of this plant have been used for folk medicine for the treatment of migraine headache, dysmenorrheal, and remove wind dampness and for Vanishes production. Production of adventitious roots in D. morbifera by in vitro cultures could be used as alternatives materials. Leaf, stem, and root segments from D. morbifera seedling were cultured on Murashige and Skoog (MS) medium supplemented with 3.0 mg/L IBA and 30 g/L sucrose. After 4 weeks of culture, the highest induction of adventitious roots was obtained from the leaf segment. Frequency of adventitious root formation on medium with various kinds of auxins (IAA, NAA, 2,4-D, and IBA) and various concentrations of IBA (0, 0.1, 0.5, 1.0, 3.0, and 5.0 mg/L) was tested. The maximum induction of adventitious root was obtained on medium with 1.0 mg/L IBA. In liquid culture, growth of root was best 1/2MS medium supplemented with 1.0 mg/L IBA and 30 g/L sucrose. Adventitious roots were cultured in 5 L bioreactor containing 1/2 MS medium supplemented with 1.0 mg/L IBA and 30 g/L sucrose and mass-production of adventitious roots was successfully achieved. This study demonstrated for the first time to produce adventitious roots in D. morbifera.

Keywords

References

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