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Comparison Between Two Detection Methods for HPV16, HPV18 and P16Ink4a Biomarkers in Diagnosis of Abnormal Cervical Cytology

  • Khazaei, Sedigheh;Izadi, Babak;Mirbahari, Seyed Ghasem;Madani, Seyed Hamid;Khosravi, Shohre Malek;Alagha, Mohsen Emami;Sajadimajd, Soraya
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.12
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    • pp.5223-5227
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    • 2016
  • Background: Cervical cancer is the second most common cancer among women in many populations. While the Pap smear is a well established screening test it suffers from both false-positive and false-negative results in diagnosis of cancers and precancerous states. In this study, immunocytochemistry of the P16 biomarker and HPV-PCR were compared for their diagnostic potential. Materials and methods: In the study, we obtained pairs of specimens from 45 women with cervical dysplasia. One sample was placed in a liquid-based solution, and processed for staining of sections with antibodies to P16. HPV-PCR was performed on the other and the results obtained were analyzed by T-test using SPSS v. 15. Results: Using HPV-PCR 71% of the samples were found to be infected with either HPV 16 or HPV 18, and the rate of infection did not have a statistically significant relationship with higher grades of dysplasia (p= 0.253). In contrast, with immunocytochemistry evaluation of P16, 64% of the specimens were positive, but the percentage of positive results significantly increased with higher grades of dysplasia (p= 0.0001). Conclusion: Employment of the P16 marker as an optional test might be preferable over HPV-PCR for cervical dysplasia in our geographical region.

Complete genome sequence of Betaproteobacteria strain GR16-43 isolated form a freshwater pond in South Korea (담수에서 분리한 Betaproteobacteria GR16-43의 유전체 염기서열 분석)

  • Choi, Ahyoung;Baek, Kiwoon;Chung, Eu Jin;Kim, Jee-Hwan;Choi, Gang-Guk
    • Korean Journal of Microbiology
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    • v.53 no.4
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    • pp.320-322
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    • 2017
  • A betaproteobacterium strain GR16-43 was isolated from a surface layer of the Geomnyong Pond in Republic of Korea by a dilution-to-extinction culturing method. We report the whole genome sequence of the strain GR16-43, which contains 4,806,848 bp with a G + C content 67.12%, and to include 4,424 protein-coding genes and 47 transfer RNA genes. The genome was determined to contain the genes encoding carbon monoxide dehydrogenase, nitrate reductase, nitrite reductase, nitric oxide reductase, and the sulfur oxidation (sox) gene cluster, highlighting the potential importance of the bacterial group represented by the strain in the cycling of inorganic elements. These results indicate that strain GR16-43 genome showed several traits indicating adaptation of the bacteria to living in freshwater environments.

Fast Stream Cipher ASC16 (고속 스트림 암호 ASC16)

  • Kim, Gil-Ho;Song, Hong-Bok;Kim, Jong-Nam;Cho, Gyeong-Yeon
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2009.05a
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    • pp.437-440
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    • 2009
  • We propose a fast stream cipher ASC16 for software implementation. ASC16 has a very simple structure with ASR(Arithmetic Shift Register), NLF(Non-Linear Filter), and NLB(Non-Linear Block), and is executed by a word. It is a stream cipher for wireless communication, which makes 32bit key streams using s-box with non-linear transformation. The processed result is almost same as SSC2, 32bit output stream cipher, developed by Zhang, Carroll, and Chan. The period is longer than SSC2, and it causes the difficulty of Correlation attack and raises security very much. The proposed ASC16 is efficiently used in the process of a fast cipher in the limited environment such as wireless communication.

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Study for Whitening Activity of Mixture of Arbutin and Oil Soluble Licorice Extract (알부틴과 유용성감초 추출물 혼합물에 의한 미백활성 연구)

  • Jang, Hye In
    • Journal of the Korean Applied Science and Technology
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    • v.36 no.2
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    • pp.635-644
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    • 2019
  • The aim of this study was to investigate whitening effect of combination between arbutine and oil soluble licorice extract. Inhibitory effects of arbutin and oil soluble licorice extract against tyrosinase activity and melanogenesis in B16 melanoma cells were assessed in vitro to determine whitening effect. MTT assay with B16 melanoma cells showed that mixture (arbutin and oil soluble) was not each concentration. Both oil soluble licorice extract and arbutin induced dose-dependent inhibition of mushroom tyrosinase activity. Various concentrations (oil soluble extract : arbutin = 1:1. 1:1.5, 1:2, 1:2.5, 1:5) of mixtures also significantly inhibited tyrosinase activity in B16 melanoma cells by 40-51%. In addition, the mixtures reduced the melanin contents of B16 melanoma cells by more than 50% at each concentration. These results suggest that mixtures of arbutin and oil soluble licorice extract are very effective whitening ingredients.

Uncontrolled Manifold Analysis of Whole Body CoM of the Elderly: The Effect of Training using the Core Exercise Equipment

  • Park, Da Won;Koh, Kyung;Park, Yang Sun;Shim, Jae Kun
    • Korean Journal of Applied Biomechanics
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    • v.28 no.4
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    • pp.213-218
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    • 2018
  • Objective: The purpose of this study was to examine the effect of the core muscle strength enhancement of the elderly on 8 weeks training using the core exercise equipment for the elderly on the ability to control the whole-body center of mass in posture stabilization. Method: 16 females (10 exercise group, 6 control group) participated in this study. Exercise group took part in the core strength training program for 8 weeks with total of 16 repetitions (2 repetitions per week) using a training device. External perturbation during standing as pulling force applied at the pelvic level in the anterior direction was provided to the subject. In a UCM model, the controller selects within the space of elemental variables a subspace (a manifold, UCM) corresponding to a value of a performance variable that needs to be stabilized. In the present study, we were interested in how movements of the individual segment center of mass (elemental variables) affect the whole-body center of mass (the performance variable) during balance control. Results: At the variance of task-irrelevant space, there was significant $test^*$ group interactions ($F_{1,16}=7.482$, p<.05). However, there were no significant main effect of the test ($F_{1,16}=.899$, p>.05) and group ($F_{1,16}=1.039$, p>.05). At the variance of task-relevant space, there was significant $test^*$ group interactions ($F_{1,16}=7.382$, p<.05). However, there were no significant main effect of the test ($F_{1,16}=.754$, p>.05) and group ($F_{1,16}=1.106$, p>.05). Conclusion: The results of this study showed that the 8 weeks training through the core training equipment for the elderly showed a significant decrease in the $Vcm_{TIR}$ and $Vcm_{TR}$. This result indicates that the core strength training affects the trunk stiffness control strategy to maintain balance in the standing position by minimizing total variability of individual segment CMs.

A Convergence Study on the Factors Affecting Teacher Efficacy among School Teachers in COVID- 19 Special Disaster Area (코로나바이러스감염증-19 특별재난 일개 지역 교사의 교사 효능감에 영향을 미치는 요인 융합 연구)

  • Kim, Eunl-Hwi;Ha, Young-Sun;Park, Young-Kyung
    • Journal of Digital Convergence
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    • v.19 no.1
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    • pp.239-248
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    • 2021
  • This study was aimed to investigate the factors influencing teacher efficacy of depression, anxiety, stress among school teachers in COVID- 19 special disaster area. The data were collected from May 9 to 16, 2020 for 123 high school teachers in D city. As a result of the study, the influential factor of teacher efficacy was satisfaction with duty (β=0.27, p=.002), economic level (β=0.18, p=.022), education (β=0.18, p=.022), subjective health state (β=0.16, p=.047), stress (β=-0.16, p=.044), gender(β=0.16, p=.042). These factors accounted for 35% in teacher efficacy. It was found that depression(β=-0.09, p=.468) and anxiety(β=-0.12, p=.320) had no significant effect on teacher efficacy. When developing a program to improve teacher efficacy, it is required to prepare measures not only to manage stress but also to improve job satisfaction.

Performance Analysis of Viewport-dependent Tiled Streaming on 16K Ultra High-quality 360-degree Video (16K 초고화질 360도 영상에서의 사용자 시점 기반 타일 스트리밍 성능 검증)

  • Jeong, Jong-Beom;Lee, Soonbin;Kim, Inae;Ryu, Eun-Seok
    • Journal of Internet Computing and Services
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    • v.22 no.3
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    • pp.1-8
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    • 2021
  • Ultra high-quality and ultra high-resolution omnidirectional 360-degree video streaming is needed to provide immersive media through head-mounted display(HMD) in virtual reality environment, which requires high bandwidth and computational complexity. One of the approaches avoiding these problems is to apply viewport-dependent selective streaming using tile-based segmentation method. This paper presents a performance analysis of viewport-dependent tiled streaming on 16K ultra high-quality 360-degree videos and 4K 360-degree videos which are widely used. Experimental results showed 42.47% of bjotegaard delta rate(BD-rate) saving on 16K ultra high-quality 360-degree video tiled streaming compared to viewport-independent streaming while 4K 360-degree video showed 26.41% of BD-rate saving. Therefore, this paper verified that tiled streaming is more efficient on ultra-high quality video.

Analysis of Amyloid Beta 1-16 (Aβ16) Monomer and Dimer Using Electrospray Ionization Mass Spectrometry with Collision-Induced Dissociation

  • Kim, Kyoung Min;Kim, Ho-Tae
    • Mass Spectrometry Letters
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    • v.13 no.4
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    • pp.177-183
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    • 2022
  • The monomer and dimer structures of the amyloid fragment Aβ(1-16) sequence formed in H2O were investigated using electrospray ionization mass spectrometry (MS) and tandem MS (MS/MS). Aβ16 monomers and dimers were indicated by signals representing multiple proton adduct forms, [monomer+zH]n+ (=Mz+, z = charge state) and [dimer+zH]z+ (=Dz+), in the MS spectrum. Fragment ions of monomers and dimers were observed using collision-induced dissociation MS/MS. Peptide bond dissociation was mostly observed in the D1-D7 and V11-K16 regions of the MS/MS spectra for the monomer (or dimer), regardless of the monomer (or dimer) charge state. Both covalent and non-covalent bond dissociation processes were indicated by the MS/MS results for the dimers. During the non-covalent bond dissociation process, the D3+ dimer complex was separated into two components: the M1+ and M2+ subunits. During the covalent bond dissociation of the D3+ dimer complex, the b and y fragment ions attached to the monomer, (M+b10-15)z+ and (M+y9-15)z+, were thought to originate from the dissociation of the M2+ monomer component of the (M1++M2+) complex. Two different D3+ complex geometries exist; two distinguished interaction geometries resulting from interactions between the M1+ monomer and two different regions of M2+ (the N-terminus and C-terminus) are proposed. Intricate fragmentation patterns were observed in the MS/MS spectrum of the D5+ complex. The complicated nature of the MS/MS spectrum is attributable to the coexistence of two D5+ configurations, (M1++M4+) and (M2+M3+), in the Aβ16 solution.

Effect of Day/Night Temperatures during Seedling Culture on the Growth and Nodes of Early Flower Cluster Set of 'Seokwang' Tomato (Lycopersicum esculentum Mill.) (육묘시의 주야간 기온이 서광 토마토의 생육 및 초기 착화 절위에 미치는 영향)

  • 김오임;정병룡
    • Journal of Bio-Environment Control
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    • v.8 no.2
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    • pp.75-82
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    • 1999
  • This study was carried out to examine the effect of day/nignt temperatures during seedling culture on the vegetative and reproductive growth of Lycopersicum esculentum ‘Seokwang’. The study was consisted of two culture stages, plug seedling production in the growth chamber and hydroponic culture of the plant in a glasshouse. Experiments were replicated over time. The germinated seedlings were raised for 33 days (experiment 1) and 35 days (experiment 2) in 4 growth chambers, each with day/night temperatures of either $25^{\circ}C$/$25^{\circ}C$, 16$^{\circ}C$/16$^{\circ}C$, 16$^{\circ}C$/$25^{\circ}C$ or $25^{\circ}C$/16$^{\circ}C$. Cool-white fluorescent lamps provided 140$\mu$mol.m$^{-2}$ .s$^{-1}$ light for 12h each day. In the second experiment, all chambers were supplied with 1000$\mu$mol.mol$^{-1}$ CO$_{2}$ during the photoperiod and had an air velocity of 0.3m.s$^{-1}$ and relative humidity of 80%. Plug seedlings raised were transplanted to rockwool slabs in a glasshouse and were grown hydroponically using the same nutrient solutions used for seedling culture for 37 days (experiment 1) and 35 days (experiment 2). Plant height was affected more by mean daily temperature than by interaction of day and night temperatures. Plant height was the highest in 16/16$^{\circ}C$ treatment. Leaf count was not affected by day and night temperatures, and the chlorophyll concentration was the highest in 16/$25^{\circ}C$ treatment. Fresh and dry weights of stem tended to be greater in treatments of constant day and night temperature. The number of node on which first and second flower clusters were set was significantly higher in 25/$25^{\circ}C$ treatment than in the other treatments. Days to flower of the first flower on the first flower cluster were the greatest in 25/$25^{\circ}C$ and the least in 16/$25^{\circ}C$ treatment. Vegetative and reproductive growth, such as height, fresh and dry weights, days to flower, and nodes of the 1st and 2nd flower cluster set were affected by day/night temperatures.

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Effects of Dietary Protein Level and Supplementation of Conjugated Linoleic Acid on Growth Performance and Meat Quality Parameters in Finishing Pigs (사료 중 단백질 수준 및 CLA(Conjugated Linoleic Acid) 첨가가 비육돈의 생산성 및 육질특성에 미치는 영향)

  • Moon, Hong-Kil;Lee, Sung-Dae;Jung, Hyun-Jung;Kim, Young-Hwa;Park, Jun-Cheol;Ji, Sang-Yun;Kim, Chong-Dae;Kwon, Oh-Sub;Kim, In-Cheul
    • Journal of Animal Science and Technology
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    • v.50 no.5
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    • pp.695-704
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    • 2008
  • This study was conducted to investigate effects of dietary crude protein(CP) level and supplementation of conjugated linoleic acid(CLA) on growth performances and meat quality parameters in finishing pigs. The experiment was designed using protein levels(11.3%, 16.0%) and CLA levels(0%, 2.5%) according to 2×2 factorial design. A total of forty-eight pigs [(Landrace×Yorkshire)×Duroc] with an average initial weight of 79±1kg were allotted to one of four dietary treatments. Each treatment had four replications of three pigs per replicate. Final body weight(P<0.05) and average daily gain(P<0.01) were lower in 11.3% CP treatments than in 16.0% CP treatments, while feed/gain was high(P<0.01) in 11.3% CP treatments compared with 16.0% CP treatments. Carcass weight was lighter in 11.3% CP treatments(P<0.001) and CLA 2.5% treatments(P<0.01) than in 16.0% CP and CLA 0% treatments, respectively. A significant interaction between CP and CLA on carcass weight was observed(P<0.01), where supplementation of 2.5% CLA to finishing diets decreased carcass weight in 16.0% CP treatments, while no difference was found in 11.3% CP treatments. Backfat thickness was thinner in 11.3% CP treatments and CLA 2.5% treatments than in 16.0% CP and CLA 0% treatments, respectively(P<0.05). A significant interaction between CP and CLA on backfat thickness was observed(P<0.001), where supplementation of 2.5% CLA to finishing diets decreased backfat thickness in 16.0% CP treatments, while no difference was found in 11.3% CP treatments. Marbling score and intramuscular fat contents were higher in 11.3% CP treatments than in 16.0% CP treatments(P<0.01). In conclusion, feeding of protein-deficient diets in finishing pigs could produce favorable pork with high marbling score and thinner backfat. On the other hand, supplementation of CLA was considered to decrease backfat thickness when diets with optimal level of crude protein were fed to finishing pigs.