• 약학회지
• /
• 제54권5호
• /
• pp.377-386
• /
• 2010

Single-dimensional (1-D) and two-dimensional (2-D) LC methods were utilized to separate peptides from various sources followed by MS/MS analysis. Two-dimensional ultra-high performance liquid chromatography is a useful tool for proteome analysis, providing a greater peak capacity than 1-D LC. The most popular 2-D LC approach used today for proteomic research combines strong cation exchange and reversed-phase LC. We have evaluated an alternative mode for 2-D LC of peptides using 2-D RP-RP nano UPLC Q-TOF Mass Spectrometry, employing reversed-phase columns in both separation dimensions. As control experiments, we identified 129 proteins in 1-D LC and 322 proteins in 2-D LC from E. coli extract peptides. Furthermore, we applied this method to rat primary hepatocyte and a total of 170 proteins were identified from 1-D LC, and 527 proteins were identified from all 2-D LC system. The in-depth protein profiling established by this 2-D LC MS/MS from rat primary hepatocyte could be a very useful reference for future applications in regards to drug induced liver toxicity.

• 한국의류학회지
• /
• 제33권1호
• /
• pp.139-148
• /
• 2009

In this study, we tried to verify the effectiveness of 3D scans of clothed participants from 20 to 24 year old men in fit analysis process. Carrying out fit analysis, we used 3D scan data and direct fit evaluation with the basic garments made as semi-fitted bodice pattern for men. The result of fit evaluation through 3D scan data showed the expert rated fitting items more positive than direct fit evaluation. Even though, generally there was a no significant difference in the response between 3D and direct fit analysis. Only there was a significant difference in the response to the specific place such as the center front line, neckline, shoulder seam, and etc. There was a no significant difference between the result of 3D scan and direct fit evaluation in the subjects' group. Also the result of assessment of 3D targeting consumers showed very positive and interested in using 3D scans for fit analysis. The ability to rotate the 3D scans for a variety of views proved to be a very effective process to analyze fit. Moreover, digital data is easily accessed at any locations and any time. Fit analysis using 3D scans could be great tool for not only fit evaluation in research but also better fitting in apparel industry.

• 한국군사과학기술학회지
• /
• 제14권6호
• /
• pp.1207-1214
• /
• 2011

Design of 500W class UMGT(Ultra Micro Gas Turbine) for power generation is conducted. Basic design parameters are obtained by cycle analysis. Off-design performances are predicted by 1D aerodynamic design and 1D performance analysis of compressor and turbine. 3D impellers are designed and 3D performance analysis is carried out to predict the performance characteristics of UMGT. 1D and 3D performance analysis show similar results. Structure analysis is conducted to select materials. Titanium Alloy is proposed for structural stability.

• 대한산업공학회지
• /
• 제40권1호
• /
• pp.84-99
• /
• 2014

In this study, determinant input-output variables are identified for calculating Data Envelopment Analysis (DEA) efficiency scores relating to evaluating the efficiency of government-sponsored research and development (R&D) projects. In particular, this study proposes a systematic framework of design and analysis of experiments, called "all possible DEAs", for pinpointing DEA determinant input-output variables. In addition to correlation analyses, two modified measures of time series analysis are developed in order to check the similarities between a DEA complete data structure (CDS) versus the rest of incomplete data structures (IDSs). In this empirical analysis, a few DEA determinant input-output variables are found to be associated with a typical public R&D performance evaluation logic model, especially oriented to a mid- and long-term performance perspective. Among four variables, only two determinants are identified : "R&D manpower" ($x_2$) and "Sales revenue" ($y_1$). However, it should be pointed out that the input variable "R&D funds" ($x_1$) is insignificant for calculating DEA efficiency score even if it is a critical input for measuring efficiency of a government-sonsored R&D project from a practical point of view a priori. In this context, if practitioners' top priority is to see the efficiency between "R&D funds" ($x_1$) and "Sales revenue" ($y_1$), the DEA efficiency score cannot properly meet their expectations. Therefore, meticulous attention is required when using the DEA application for public R&D performance evaluation, considering that discrepancies can occur between practitioners' expectations and DEA efficiency scores.

• 미생물학회지
• /
• 제36권4호
• /
• pp.267-272
• /
• 2000

차풀(Cassia nomame)의 뿌리혹 공생세균인 Bradyrhizobium sp.(Cassia) CN9135의 nodD1 유전자를 중합효소 연쇄반응을 통해 크로닝하여 그 염기서열을 조사하였다. 염기서열로부터 유추된 NodD1 단백질은 Bradyrhizobium elkanii와 가장 높은 95%의 상동성을 나타내었다. 뿐만 아니라 nodD2 사이의 intergenic space 부위의 염기서열도 B. japonicum을 포함하는 다른 bradyrhizobia와는 상동성이 거의 없었으나 B. elkanii와는 88%의 높은 상동성을 나타내었다. 우리의 실험 결과는 Bradyrhizobium sp.(Cassia)가 B. elkanii와 매우 근연한 관계임을 보여 주고 있다.

• 한국도로학회논문집
• /
• 제18권1호
• /
• pp.73-83
• /
• 2016

PURPOSES : The purpose of this study is to develop a method for improving the accuracy of analysis results obtained from a two-dimensional (2-D) numerical analysis model of continuously reinforced concrete pavement (CRCP). METHODS : The analysis results from the 2-D numerical model of CRCP are compared with those from more rigorous three-dimensional (3-D) models of CRCP, and the relationships between the results are recognized. In addition, the numerical analysis results are compared with the results obtained from field experiments. By performing these comparisons, the calibration factors used for the 2-D CRCP model are determined. RESULTS : The results from the comparisons between 2-D and 3-D CRCP analyses show that with the 2-D CRCP model, concrete stresses can be overestimated significantly, and crack widths can either be underestimated or overestimated by a slight margin depending on the assumption of plane stress or plane strain. The behaviors of crack width in field measurements are comparable to those obtained from the numerical model of CRCP. CONCLUSIONS : The accuracy of analysis results from the 2-D CRCP model can be improved significantly by applying calibration factors obtained from comparisons with 3-D analyses and field experiments.

• 통합자연과학논문집
• /
• 제11권1호
• /
• pp.25-29
• /
• 2018

Protein phosphatase manganese dependent 1D (PPM1D), a Ser/Thr protein phosphatise, play major role in the cancer tumorigenesis of various tumors including neuroblastoma, pancreatic adenocarcinoma, medulloblastoma, breast cancer, prostate cancer and ovarian cancer. Hence, analysis on the structural features required for the formation of PPM1D-inhibitor complex becomes essential. In this study, we have performed molecular docking of SL-175 and -176 and protein-protein docking of CDC5L with PPM1D. On analysing the docked complexes, we have identified the important residues involved in the formation of protein-ligand complex. Research concentrating on these residues could be helpful in understanding the pathophysiology of various tumors related to PPM1D.

• 한국철도학회논문집
• /
• 제13권2호
• /
• pp.139-146
• /
• 2010

본 연구에서는 복잡한 3차원 유한요소모델 충돌거동과 등가인 1차원 동역학 모델링 방법을 개발하기 위하여 새로운 1-D 모델링 방법을 제안하였다. 충돌 거동을 잘 일치시키기 위해서는 충돌 시너지를 주로 흡수하는 압괴 구간의 특성을 정확하게 모델링하는 것이 중요하다. KHST 편성차량을 대상으로 3차원 유한요소 모델의 차체단면에 설정한 단면 옵션으로 충돌해석 시 차체 단면에 작용하는 충격하중과 변형을 추출하여 새로운 1차원 충돌동역학 모델의 스프링요소 특성으로 모델링하고, 국내철도차량 안전기준의 열차 대 열차 충돌사고 각본으로 수치해석을 수행하였다. 두 모델의 에너지 흡수량, 충돌 가속도, 충격하중-변형 등을 비교한 결과가 잘 일치하였다.

• 한국전자파학회논문지
• /
• 제13권2호
• /
• pp.136-145
• /
• 2002

본 논문에서는 단일 PBG 구조의 전송특성을 ABCD 파라미터로 모델링하여 연속한 N개의 PBG 구조에 대한 전송특성의 이론적 분석을 용이하게 하였다. 또한 무한 PBG구조에 대한 분산 다이어그램을 도출하였으며, 연속된 N개의 주기적 PBG구조에서 주파수 특성과 분산 다이어그램과의 상호연관성을 고찰하기 위해 N=10일 경우의 PBG 구조를 이론, 회로 시뮬레이션, EM 시뮬레이션을 이용하여 해석하였는데 세 결과 그래프가 거의 일치하였다. 이러한 방법론을 적용할 경우 주어진 PBG 구조의 분석(Analysis)이 용이할 뿐만 아니라 특정한 저지대역의 설계(Synthesis)도 용이해진다.

• Maxillofacial Plastic and Reconstructive Surgery
• /
• 제30권6호
• /
• pp.546-553
• /
• 2008

Purpose: The purpose of this study was to estimate validity of posterior anterior cephalometric and 3D-CT for orbital canting analysis. Materials and methods: Three trained observers classified two patients group using standardized frontal photographs of facial asymmetry patients. Group A consisted of patients with facial asymmetry and orbital canting(n=19), and group B consisted of patients with only facial asymmetry(n=43). Orbital canting was measured with line of bilateral inferior orbitale. Orbital canting measurement was done with posterior anterior cephalometric and 3D-CT. Each horizontal reference line was established by bilateral GWSO(cephalometric), FZS(3D-CT). Maxillary canting and mandibular deviation angle were also measured and analyzed with orbital canting. Results: The mean orbital canting was $3.03{\pm}1.00^{\circ}$ in Group A and $1.11{\pm}0.76^{\circ}$ in Group B in frontal photograph. The mean orbital canting was $1.20{\pm}0.74^{\circ}$ in group A and $1.22{\pm}0.65^{\circ}$ in group B by cephalometric analysis(p>0.05). In 3D-CT, orbital canting was almost paralleled with horizontal reference line. The orbital canting, maxillay canting and mandibular deviation between two groups showed no significant differences except madibular deviation in 3D-CT. Conclusion: Common analysis of posterior anterior cephalometric and 3D-CT is not valide method to evaluate orbital canting for facial asymmetry patients with orbital canting.