• The Korean Journal of Physiology and Pharmacology
• /
• 제7권1호
• /
• pp.29-31
• /
• 2003

It is well known that different expression of Apo-1/Fas (CD95) plays important roles in various tumors and hepatocellular carcinoma (HCC) pathogenesis. Apo-1/Fas mediated apoptosis is one of the important pathways of apoptosis and is known to mediate apoptotic cell death by fas ligand (FasL). To examine the possible relationship between Apo-1/Fas gene polymorphism and HCC susceptibility, MvaI restriction fragment length polymorphism (RFLP) of Apo-1/Fas gene was examined in 94 Korean HCC patients and 240 control subjects. No statistically significant difference in the genotypic distribution and allelic frequencies was found between the control and the HCC. It is, therefore, concluded that Apo-1/Fas gene polymorphism is not associated with HCC susceptibility. Further studies are needed in order to clarify the relationships between genotypes of Apo-1/Fas gene and HCC pathogenesis.

• 한국균학회지
• /
• 제22권3호
• /
• pp.254-259
• /
• 1994

P. chrysosporium BKM-F-1767 및 C. subvermispora FP90031-SP. 균사를 이용하여 ligninase와 laccase 활성제고를 위한 배앙방법을 검토한 결과는 다음과 같다. P. chrysosporium의 왕복진탕배양시 (150 rpm) 배양액량은 45-60 ml가 ligninase 활성제고에 적합하였으며 60 ml 배양은 최대활성 출현일이 빨리 나타났다. C. subvermispora의 정치배양시, 2% M.E.+0.1% Y.E.+0.1% T.W.20+1mMVA 처리에서 ligninase 활성이 가장 높았고 laccase의 활성은 2% M.E.+0.1% Y.E.+0.1% T.W.20+6 mMBA에서 최고 수준을 나타내었다.

• 조명전기설비학회논문지
• /
• 제23권1호
• /
• pp.85-91
• /
• 2009

본 논문에서는 배전계통에 사용되는 케이블 권선형 변압기에 전력계통에서 발생되는 서지나 고장전류에 의해서 변압기의 고압측 코일에 유도되는 전자력의 크기를 유한요소 전자계해석 프로그램(FLUX2D)을 이용하여 해석하고, 기계진동방정식을 이용하여 케이블형 권선의 고유진동주파수를 분석하였다. 변압기모델로서 1(MVA), 22,900/220([V]) 단상 외철형 Cable형을 선정하여, 권선의 각방향의 전자력을 분석하고, 고유진동 mode를 Transfer Matrix Method를 이용하여 계산하고 상용 프로그램(ANSYS)을 이용하여 분석한 결과와 비교하였다. 진동모드의 분석한 결과 제시한 수치해석 방법은 공학적인 오차범위 안에서 충분히 수용할 정도의 정확도를 보여주었다. 변압기 권선의 전자력과 진동모드 분석결과는 변압기의 절연설계 및 단락기계력에 의한 절연 구조 설계를 위한 유용한 자료로 활용될 수 있다.

• 전력전자학회논문지
• /
• 제12권5호
• /
• pp.424-431
• /
• 2007

본 논문은 발전소의 이차공기송풍기를 가변 회전수 제어하여 소내 소비 전력을 절감하고 전동기의 직입기동에 의한 스트레스를 줄이기 위하여 실증 적용된 200MW 석탄화력 발전소 순환 유동층 보일러 이차 공기 송풍기 구동용 H-브릿지 멀티레벨 인버터에 관한 것이다. H-브릿지 멀티레벨 인버터는 독립적으로 절연된 직류 부를 갖는 저전압의 단상 인버터(셀 인버터)를 다수 직렬 접속하여 고전압 3상 전압을 출력하는 Cascaded H-브릿지 전압형 인버터이다. 전력연구원은 6.6kV, 1MVA급 인버터 개발, 발전소에 설치, 보일러 점화 전/후 시험 및 계통 병입 후 부하 시험 등을 국내 최초로 성공적으로 완료하였다. 현재 200MW 석탄화력 발전소 이차 공기 송풍기 구동용 인버터는 상업 운전 중에 있다. 본 논문에서는 개발된 H-브릿지 멀티레벨 인버터, 기존 발전 설비와 신규 인버터 시스템의 인터페이스 부분에 해당하는 보일러 DCS(Distributed Control System : 분산 제어 시스템) 로직, 계통 병입 후 시운전 결과에 대하여 상세히 기술하였다.

• 한국산업정보학회논문지
• /
• 제25권1호
• /
• pp.25-30
• /
• 2020

3상 다층 고온 초전도 동축 케이블은 초전도 선재 사용량의 감소 및 케이블의 소형화와 같은 이점 때문에 활발히 연구되고 있다. 3상 다층 고온 초전도 동축 케이블의 전기적 특성은 기존 초전도 케이블과 차이를 가지므로 실제 시스템에 적용하기 위해 충분한 분석이 필요하다. 본 논문에서는 PSCAD/EMTDC 기반 시뮬레이션을 통하여 22.9 kV, 60 MVA급 3상 다층 고온 초전도 동축 케이블을 모델링하고 과도 특성을 분석하였다. 결과적으로 3상 다층 고온 초전도 동축 케이블에서 고장전류가 발생하면 대부분의 고장전류가 구리 포머층을 통해 우회한다. 이때, 케이블 전체 온도는 약 5 K 증가하였다. 본 논문을 통해 3상 다층 고온 초전도 동축 케이블의 과도 상태에 대한 신뢰성을 확인할 수 있으며 향후 케이블의 실 계통 적용에 도움이 될 수 있다.

• 전기전자학회논문지
• /
• 제24권1호
• /
• pp.216-224
• /
• 2020

본 논문은 계통 연계 기준인 Low Voltage Ride Through(LVRT) 및 High Voltage Ride Through(HVRT) 기능을 평가하기 위한 시험 장비의 임피던스 설계 방법을 제안한다. LVRT/HVRT 시험 장비는 계통 연계 규정에 명시되어 있는 계통 사고 전압을 일정시간 동안 발생시킬 수 있어야 하며 설계 사양에 맞게 사고전류의 크기를 제한해야 한다. 본 논문에서는 LVRT/HVRT 동작 시 탭 변환 단권변압기 시험 장비의 등가 모델을 기반으로 계통 연계 규정을 만족하기 위한 단권변압기의 임피던스를 설계한다. 제안하는 설계 방법을 이용하여 LVRT/HVRT 시험 시 요구되는 다양한 사고전압을 출력할 수 있는 시험장비의 설계를 위한 탭 간의 임피던스 설계 과정을 설명한다. 제안하는 설계 방법의 타당성을 검증하기 위하여, 10MVA급 LVRT/HVRT 시험 장비의 설계 과정을 설명하고 시뮬레이션을 통하여 확인하였다.

• Journal of Ginseng Research
• /
• 제42권3호
• /
• pp.277-287
• /
• 2018

Background: Temperature is an essential condition in red ginseng processing. The pharmacological activities of red ginseng under different steam temperatures are significantly different. Methods: In this study, an ultrahigh-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was developed to distinguish the red ginseng products that were steamed at high and low temperatures. Multivariate statistical analyses such as principal component analysis and supervised orthogonal partial least squared discrimination analysis were used to determine the influential components of the different samples. Results: The results showed that different steamed red ginseng samples can be identified, and the characteristic components were 20-gluco-ginsenoside Rf, ginsenoside Re, ginsenoside Rg1, and malonyl-ginsenoside Rb1 in red ginseng steamed at low temperature. Meanwhile, the characteristic components in red ginseng steamed at high temperature were 20R-ginsenoside Rs3 and ginsenoside Rs4. Polar ginsenosides were abundant in red ginseng steamed at low temperature, whereas higher levels of less polar ginsenosides were detected in red ginseng steamed at high temperature. Conclusion: This study makes the first time that differences between red ginseng steamed under different temperatures and their ginsenosides transformation have been observed systematically at the chemistry level. The results suggested that the identified chemical markers can be used to illustrate the transformation of ginsenosides in red ginseng processing.

• Journal of Microbiology and Biotechnology
• /
• 제29권10호
• /
• pp.1656-1664
• /
• 2019

Isoprene has the potential to replace some petroleum-based chemicals and can be produced through biological systems using renewable carbon sources. Ralstonia eutropha can produce value-added compounds, including intracellular polyhydroxyalkanoate (PHA) through fatty acid and lipid metabolism. In the present study, we engineered strains of R. eutropha H16 and examined the strains for isoprene production. We optimized codons of all the genes involved in isoprene synthesis by the mevalonate pathway and manipulated the promoter regions using pLac and pJ5 elements. Our results showed that isoprene productivity was higher using the J5 promoter ($1.9{\pm}0.24{\mu}g/l$) than when using the lac promoter ($1.5{\pm}0.2{\mu}g/l$). Additionally, the use of three J5 promoters was more efficient ($3.8{\pm}0.18{\mu}g/l$) for isoprene production than a one-promoter system, and could be scaled up to a 5-L batch-cultivation from a T-flask culture. Although the isoprene yield obtained in our study was insufficient to meet industrial demands, our study, for the first time, shows that R. eutropha can be modified for efficient isoprene production and lays the foundation for further optimization of the fermentation process.

• Journal of Trauma and Injury
• /
• 제20권1호
• /
• pp.19-25
• /
• 2007

Purpose: Trauma surgery is not an official medical specialty in the Republic of Korea (South Korea). Thus, a trauma victim transported to an emergency room (ER) is resuscitated and surveyed by an intern, a resident, or an emergency physician (EP) at first. Currently an operative management is decreasing because of multiple factors. Nevertheless, trauma surgery is the key for some patients. Does the EP's treatment in the ER delay the surgeon's emergency operation? Methods: A retrospective study was performed for trauma victims who underwent trauma surgery from March 2004 to February 2005 in a local emergency center of Daegu-city. We reviewed the medical records and analyzed the trauma victim's age, sex, cause of injury, method of transport, time from the trauma to the operation, EP's treatment, surgical department, mortality, and injury severity score (ISS). Results: Of the 223 trauma victims included in this study, males were predominant (83.4%). The mean age was 37.98 years of age. The main Causes of trauma were trauma NOS (not otherwise specified) and motor vehicle accidents (MVA). The main methods of transport was privately owned automobile. The mean time from trauma to operation was 617.46 min. The mean ISS was 7.67. Trauma surgery with the EP's treatment group included 40 trauma victims with higher ISS, and the time from trauma to operation was shorter than it was for the 183 trauma victims not in that group. Conclusion: The EP's treatment of high-ISS multiple-injury trauma victims can shorten the time from trauma to trauma surgery and will help the surgical department treatment. In the trauma care system of the Republic of Korea, and increased role should be encouraged for emergency physician.

• BMB Reports
• /
• 제40권6호
• /
• pp.861-869
• /
• 2007

The enzyme 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR; EC1.1.1.34) catalyzes the first committed step of isoprenoids biosynthesis in MVA pathway. Here we report for the first time the cloning and characterization of a full-length cDNA encoding HMGR (designated as CgHMGR, GenBank accession number EF206343) from hazel (Corylus avellana L. Gasaway), a taxol-producing plant species. The full-length cDNA of CgHMGR was 2064 bp containing a 1704-bp ORF encoding 567 amino acids. Bioinformatic analyses revealed that the deduced CgHMGR had extensive homology with other plant HMGRs and contained two transmembrane domains and a catalytic domain. The predicted 3-D model of CgHMGR had a typical spatial structure of HMGRs. Southern blot analysis indicated that CgHMGR belonged to a small gene family. Expression analysis revealed that CgHMGR expressed high in roots, and low in leaves and stems, and the expression of CgHMGR could be up-regulated by methyl jasmonate (MeJA). The functional color assay in Escherichia coli showed that CgHMGR could accelerate the biosynthesis of $\beta$-carotene, indicating that CgHMGR encoded a functional protein. The cloning, characterization and functional analysis of CgHMGR gene will enable us to further understand the role of CgHMGR involved in taxol biosynthetic pathway in C. avellana at molecular level.