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http://dx.doi.org/10.1016/j.jgr.2017.02.003

Remarkable impact of steam temperature on ginsenosides transformation from fresh ginseng to red ginseng  

Xu, Xin-Fang (School of Chinese Materia Medica, Beijing University of Chinese Medicine)
Gao, Yan (School of Chinese Materia Medica, Beijing University of Chinese Medicine)
Xu, Shu-Ya (School of Chinese Materia Medica, Beijing University of Chinese Medicine)
Liu, Huan (School of Chinese Materia Medica, Beijing University of Chinese Medicine)
Xue, Xue (School of Chinese Materia Medica, Beijing University of Chinese Medicine)
Zhang, Ying (School of Chinese Materia Medica, Beijing University of Chinese Medicine)
Zhang, Hui (School of Chinese Materia Medica, Beijing University of Chinese Medicine)
Liu, Meng-Nan (School of Chinese Materia Medica, Beijing University of Chinese Medicine)
Xiong, Hui (School of Chinese Materia Medica, Beijing University of Chinese Medicine)
Lin, Rui-Chao (School of Chinese Materia Medica, Beijing University of Chinese Medicine)
Li, Xiang-Ri (School of Chinese Materia Medica, Beijing University of Chinese Medicine)
Publication Information
Journal of Ginseng Research / v.42, no.3, 2018 , pp. 277-287 More about this Journal
Abstract
Background: Temperature is an essential condition in red ginseng processing. The pharmacological activities of red ginseng under different steam temperatures are significantly different. Methods: In this study, an ultrahigh-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was developed to distinguish the red ginseng products that were steamed at high and low temperatures. Multivariate statistical analyses such as principal component analysis and supervised orthogonal partial least squared discrimination analysis were used to determine the influential components of the different samples. Results: The results showed that different steamed red ginseng samples can be identified, and the characteristic components were 20-gluco-ginsenoside Rf, ginsenoside Re, ginsenoside Rg1, and malonyl-ginsenoside Rb1 in red ginseng steamed at low temperature. Meanwhile, the characteristic components in red ginseng steamed at high temperature were 20R-ginsenoside Rs3 and ginsenoside Rs4. Polar ginsenosides were abundant in red ginseng steamed at low temperature, whereas higher levels of less polar ginsenosides were detected in red ginseng steamed at high temperature. Conclusion: This study makes the first time that differences between red ginseng steamed under different temperatures and their ginsenosides transformation have been observed systematically at the chemistry level. The results suggested that the identified chemical markers can be used to illustrate the transformation of ginsenosides in red ginseng processing.
Keywords
MVA; red ginseng; steaming temperature; transformation; UPLC-QTOF-MS/MS;
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