• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.107-108
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• 2003

This study was conducted to investigate the effects of dilution rate and stored temperature of semen at 5, 25 and 35$^{\circ}C$ on fertility in liquid rooster semen. At 5$^{\circ}C$ cold temperature, no significant difference were found in sperm mobilities on dilution rate(1:1, 1:3, 1.6) among treatments. Sperm mobility for the conservation of 3 hours at 25∼35$^{\circ}C$ were significantly higher for 1:3 and 1:6 dilution rate(semen：diluent) groups than for 1:1 dilution rate group(P<0.05). In Fertility results after artificial insemination with the conservation of 3 hours at 5∼25$^{\circ}C$ temperature, no significant difference were found in fertility on dilution rate among treatments. Fertilities after artificial insemination with the conservation of 3 hours at 35$^{\circ}C$ were significantly higher for 1.3 and 1:6 dilution rate(semen：diluent) groups than for 1：1 dilution rate group(P<0.05).

• Proceedings of the Korea Society of Poultry Science Conference
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• 2002.11a
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• pp.116-117
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• 2002

This study was conducted to investigate the effects of dilution rate and stored time of semen at 5, 25 and 35$^{\circ}C$ on mobility in liquid rooster semen. At 5$^{\circ}C$ cold temperature, no significant difference were found in sperm mobilities on dilution rate and stored time among treatments stored at 5$^{\circ}C$. Sperm mobility for the conservation of 1 hours at 25$^{\circ}C$ and 3 hours at 35$^{\circ}C$ were significantly higher for 1：2 and 1：3 dilution rate(semen： diluent) groups than for 1：1 dilution rate group(P〈0.05), In Fertility results after artificial insemination with different number of sperm per dole, fertilization rate of liquid rooster semen diluted with skim milk-glucose solution were 90.67, 94.00, 96.00, and 98.67% for 0.2${\times}$10$\^$8//dose, 0.4${\times}$10$\^$8//dose, 1.0${\times}$10$\^$8//dose, and 2.0${\times}$10$\^$8//dose groups, respectively. To have more than 90% fertility, 0.2${\times}$10$\^$8/ sperm per dose for the artificial insemination (AI) could be used, And to have more 94% fertility, 0.4${\times}$10$\^$8/ sperm per dose AI could be used practically.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2000.11a
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• pp.103-104
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• 2000

우리나라의 악취배출 허용기준에는 직접관능법, 공기희석관능법 및 기기분석법에 의한 측정과 규제 기준을 제시하고 있다. 직접관능법의 경우 신속하고 간편하여 현장에서 바로 판정을 내릴 수 있다는 점에서 부지경계선상에서의 악취의 평가수단으로 이용되어 현재까지 많은 평가서에 측정결과를 제시하고 있다. 그러나 직접관능법에 의한 평가결과가 규제치를 넘어 법적 조치가 이루어진 사업장은 거의 없는 듯하다. (중략)

• Applied Biological Chemistry
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• v.39 no.6
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• pp.488-493
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• 1996

As a first step of pesticide runoff studies, runoff losses of captafol were measured under natural rainfall conditions in apple orchard area. The maximum concentration of captafol was 180 ppb at 5 th sampling period when the rainfall occurred within 24 hours after captafol was applied, and the concentration of samples from other periods was below than 20 ppb. Total runoff loss of captafol was below 0.1%. About 10 fold of dilution factor was observed at the merging point with stream near outlet from orchard and about 50 fold was observed at the next merging point which is located further down. Therefore, captafol will not harm the aquatic organisms due to dilution factor$(10{\sim}50\;fold)$ and rapid hydrolytic degradation rate even when it was run off into a stream nearby.

• Korean Journal of Poultry Science
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• v.44 no.1
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• pp.59-65
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• 2017

This study examined factors affecting the analysis of motility of chicken semen. The viability of spermatozoa was estimated using varying dilution ratios and supplementation with BSA or fatty acid free (FAF)-BSA as protein sources in semen diluent. Fresh semen was examined after preparing dilutions in beltsvile poultry semen extender (BPSE) of 1/8, 1/16 and 1/32 at $25^{\circ}C$. The motility of incubated semen at each dilution was observed at 3 min (89.9%, 69.9% and 53.2%), 30 min (86.7%, 71.4% and 51.7%), 1 h (89.5%, 74.0% and 53.5%) and 3 h (78.5%, 66.5% and 45.7%), respectively. The addition of BSA or FAF-BSA to BPSE diluent significantly increased the viability of semen in 1/32 dilution with results of 53.2% (control), 84.8% (BSA) and 92.9% (FAF-BSA) (p<0.05). This phenomenon was also observed in the dilution of frozen semen, where FAF-BSA treatment increased the viability of thawed semen from 17.6% to 34.0% in a 1/8 dilution (p<0.05). When the protein sources were used in the dilution, the survival rates of diluted chicken semen were also increased with time lapse. These results show that FAF-BSA may act to protect chicken semen and is suitable as a basic component of chicken semen diluent for the method of analyzing rooster semen after freezing.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2000.04a
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• pp.127-128
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• 2000

악취 측정은 악취의 배출 허용을 판정하는데 대단히 중요한 기술이라 할 수 있다. 우리나라의 경우 악취배출 허용기준은 대기환경보전법에 명시되어 있으며 그 규제기준의 준법성 여부는 직접 관능법에 의한 악취 세기, 희석배율(악취농도) 및 기기측정에 의한 악취성분의 농도에 의해 판정하고 있다. 최근 악취민원이 전국으로 확산되면서 악취 배출원을 갖는 사업장과 악취오염의 피해를 입는 주민의 입장에서 악취의 허용배출의 정도에 대한 견해차이가 부각되면서 악취의 측정이나 시료채취에 대한 합리성이 요구되고 있다.(중략)

• Korean Journal of Food Science and Technology
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• v.25 no.1
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• pp.69-77
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• 1993

Substrate removal efficiency of industrial wastewater from dye plant was investigated with fluidized-bed biofilm reactor(FBBR). Wastewater was diluted by 2, 3 and 6 times for experiment. When F/M ratio was increased 0.2 to 0.3, substrate removal efficiency of wastewater was rapidly decreased in all dilution ratio. Substrate removal efficiency was increased with dilution ratio in same F/M ratio, with hydraulic retention time. In case of 6 times diluted wastewater, below 0.2 F/M ratio, removal efficiency of BOD was $90{\sim}97%$. For reactor design, the parameters such as Y, $k_d$ and r was obtained as follows: $Y=0.3365\;k_d=0.03782\;day^{-1}\;r=0.997$ in 2 times diluted $Y=0.3341\;k_d=0.02750\;day^{-1}\;r=0.996$ in 3 times diluted $Y=0.3365\;k_d=0.03434\;day^{-1}\;r=0.998$ in 6 times diluted

• Journal of Korean Society of Environmental Engineers
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• v.35 no.2
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• pp.137-143
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• 2013

This study is to develop the production method of stepwise dilution gas for the evaluation of complex odor concentration by orifice tube. The basic orifice tube for 10 and 30 times of dilution sample was made at first, and with the combination of the basic orifice tubes we can continuously manufacture the stepwise dilution sample gas for air dilution sensory test ; 10, 30, 100, 300, 1,000, 3,000 times etc. The hole size of orifice tube was 0.84 mm for 10 times of dilution sample, and was 0.34 mm for 30 times. Dilution sample gas made with the basic orifice tube have an excellent reproducibility, 2%RSD. In addition, over 90% of correlation was shown between the sample made by the orifice tube and the sample by the syringe dilution method. Because there was no concentration drift of dilution gas with changes of connected pump flow, the basic orifice tube could be mounted directly with a vacuum suction box, and could be used simply as a tool for the evaluation of odor, especially on site.

• Korean Journal of Poultry Science
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• v.45 no.3
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• pp.193-200
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• 2018

The purpose of this study was to evaluate the influence and safety of spraying Farm Clean-S (FC-S) containing 70% Chamaecyparis obtusa essential oil on the elimination of red mite in laying hens. In total, 300 laying hens (ISA brown) aged 72 weeks and infected with red mite were assigned to individual cages for five treatments with five replicates for 18 days. Treatments consisted of distilled water (negative control; NC), commercial spray product (positive control; PC), FC-S 0 (original concentration), FC-S 50 (50 times diluted) and FC-S 100 (100 times diluted). The number of red mites, egg production and weight of each group were evaluated daily throughout the whole experimental period. Egg quality and blood biochemical parameters were measured at the end of the experiment. Safety tests were confirmed by feed intake, respiration, lethargy, diarrhea and mortality symptoms after the feeding trial. As the result, more than 80% of red mites were eliminated by the FC-S 0 spray treatment, and FC-S 50 also showed a higher elimination percentage than did PC (P<0.01). However, the elimination effect of the FC-S 100 spray was lower than that of the PC, but higher than that of the NC (P<0.01). These results confirmed that the optimum FC-S dilution rate ranged from 0~50 times with distilled water to eliminate the red mite effectively. The performance, egg quality, blood parameter and safety tests were not significantly different among treatments. Thus, FC-S 0 and FC-S 50 spray treatments can effectively and sustainably control red mite infestations without detrimental effects on the performance, egg quality and plasma biochemical properties of laying hens.

• Journal of agriculture & life science
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• v.46 no.5
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• pp.47-55
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• 2012

Polyclonal antisera against regional (abdominal and subcutaneous) fats were developed to reduce body fat in rats. Isolation and culture of abdominal and subcutaneous adipocytes of rats were performed for analyzing lactate dehydrogenase (LDH) concentration. At the level of 1:1,000 dilution, little antibody reactivity appeared in non-immunized serum whereas both of antisera against abdominal (AAb) and subcutaneous adipocyte plasma membrane proteins (SAb) had relatively strong reactivity till the level of 1:128,000 dilutions. Compared with regional fats, extremely low reactivities of AAb and SAb were detected with PMP of the organs (p<0.001). Both AAb and SAb were most strongly reacted with each adipocyte plasma memebrane proteins and showed statistically (p<0.01) higher cross-reactivities compared with non-immunized serum based on LDH analysis. In conclusion, these results may indicate that the present polyclonal antibodies against regional inedible adipocyte plasma membrane proteins are well developed and have safety in cross-reactivities with body organs.