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Development of Polyclonal Antibodies to Abdominal and Subcutaneous Adipocyte for Reducing Body Fat of Rat  

Choi, Chang-Weon (Department of Animal Resources, Daegu University)
Park, Dong-Woo (Department of Animal Resources, Daegu University)
Jo, Ik-Hwan (Department of Animal Resources, Daegu University)
Oh, Young-Kyoon (National Institute of Animal Science, RDA)
Chang, Sun-Sik (National Institute of Animal Science, RDA)
Choi, Nag-Jin (Department of Animal Science, Chonbuk National University)
Kam, Dong-Keun (Cargill Agri Purina, Inc.)
Baek, Kyoung-Hoon (College of Biotechnology, Yeungnam University)
Publication Information
Journal of agriculture & life science / v.46, no.5, 2012 , pp. 47-55 More about this Journal
Abstract
Polyclonal antisera against regional (abdominal and subcutaneous) fats were developed to reduce body fat in rats. Isolation and culture of abdominal and subcutaneous adipocytes of rats were performed for analyzing lactate dehydrogenase (LDH) concentration. At the level of 1:1,000 dilution, little antibody reactivity appeared in non-immunized serum whereas both of antisera against abdominal (AAb) and subcutaneous adipocyte plasma membrane proteins (SAb) had relatively strong reactivity till the level of 1:128,000 dilutions. Compared with regional fats, extremely low reactivities of AAb and SAb were detected with PMP of the organs (p<0.001). Both AAb and SAb were most strongly reacted with each adipocyte plasma memebrane proteins and showed statistically (p<0.01) higher cross-reactivities compared with non-immunized serum based on LDH analysis. In conclusion, these results may indicate that the present polyclonal antibodies against regional inedible adipocyte plasma membrane proteins are well developed and have safety in cross-reactivities with body organs.
Keywords
Body fat reduction; Antibody; Adipocyte; Rat; Cross-reactivity;
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Times Cited By KSCI : 3  (Citation Analysis)
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