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http://dx.doi.org/10.5536/KJPS.2017.44.1.59

The Effects of Supplementation of BSA or Fatty Acid Free BAS on the Motility of Fresh or Cryopreserved Rooster Spermatozoa  

Kim, Sung Woo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Kim, Min Soo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Yu, Yeonhui (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Kim, Chan-Lan (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Jeon, Ik Soo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Kim, Chongdae (Poultry Science Division, National Institute of Animal Science, RDA)
Publication Information
Korean Journal of Poultry Science / v.44, no.1, 2017 , pp. 59-65 More about this Journal
Abstract
This study examined factors affecting the analysis of motility of chicken semen. The viability of spermatozoa was estimated using varying dilution ratios and supplementation with BSA or fatty acid free (FAF)-BSA as protein sources in semen diluent. Fresh semen was examined after preparing dilutions in beltsvile poultry semen extender (BPSE) of 1/8, 1/16 and 1/32 at $25^{\circ}C$. The motility of incubated semen at each dilution was observed at 3 min (89.9%, 69.9% and 53.2%), 30 min (86.7%, 71.4% and 51.7%), 1 h (89.5%, 74.0% and 53.5%) and 3 h (78.5%, 66.5% and 45.7%), respectively. The addition of BSA or FAF-BSA to BPSE diluent significantly increased the viability of semen in 1/32 dilution with results of 53.2% (control), 84.8% (BSA) and 92.9% (FAF-BSA) (p<0.05). This phenomenon was also observed in the dilution of frozen semen, where FAF-BSA treatment increased the viability of thawed semen from 17.6% to 34.0% in a 1/8 dilution (p<0.05). When the protein sources were used in the dilution, the survival rates of diluted chicken semen were also increased with time lapse. These results show that FAF-BSA may act to protect chicken semen and is suitable as a basic component of chicken semen diluent for the method of analyzing rooster semen after freezing.
Keywords
rooster semen; Ogye; cryopreservation; BSA; dilution;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Blesbois E, Grasseau I, Seigneurin F, Mignon-Grasteau S, Saint Jalme M, Mialon-Richard MM 2008 Predictors of success of semen cryopreservation in chickens. Theriogenology 69: 252-261.   DOI
2 Choi JS, Kim SW, Shin DB, Ko YG, Do YJ, Kim DH, Kong IK, Park SB 2012 Effects of N-methylacetamide on the viability, fertility and hatchability of cryopreserved Ogye (Korean native black fowl) semen. Korean J Poult Sci 39:291-295.   DOI
3 Choi JS, Shin DB, Ko YG, Do YJ, Byun M, Park SB, Seong HH, Kim H, Kong IK, Kim SW 2013 Effects of kinds of cryoprotectants on the characteristics of frozen fowl semen. Korean J Poult Sci 40:171-178.   DOI
4 Farrel PB, Presicce GA, Brockett CC, Foote RH 1998 Quantification of bull sperm characteristics measured by computerassisted sperm analysis (CASA) and the relationship to fertility. Theriogenology 49:871-879.   DOI
5 Gill SP, Buss EG, Mallis RJ 1996 Cryopreservation of rooster semen in thirteen and sixteen percent glycerol. Poult Sci 75:254-256.   DOI
6 Hanzawa S, Niinomi T, Miyata T, Tsutsui M, Tajima A 2010 Cryopreservation of chicken semen using N-methylacetamide as cryoprotectant. Jp Poult Sci 47:J27-J32.
7 Hammerstedt RH, Graham JK 1992 Cryopreservation of poultry sperm: The enigma of glycerol. Cryobiology 29:26-38.   DOI
8 Harrison RA, Dott HM, Foster GC 1982 Bovine serum albumin, sperm motility and the "dilution effect". J Exp Zoo 222:81-88.   DOI
9 Holsberger DR, Donoghue AM, Froman DP, Ottinger MA 1998 Assessment of ejaculated quality and sperm characteristics in turkeys: Sperm mobility phenotype is independent of time. Poult Sci 77:1711-1717.   DOI
10 King LM, Holsberger DR, Donoghue AM 2000 Correlation of CASA velocity and linearity parameters with sperm mobility phenotype in turkeys. J Androl 21:65-71.
11 Lake PE 1986 The history and future of the cryopreservation of avian germ plasm. Poult Sci 65:1-15.   DOI
12 Lake PE, Ravie O 1984 An exploration of cryoprotective compounds for fowl spermatozoa. Br Poult Sci 25:145-150.   DOI
13 Makler A 1980 The improved 10 micrometer chamber for rapid sperm count and motility evalutation. Fertil Stril 33:337-338.   DOI
14 Neville WJ, Macpherson JW, Reinhart B 1971 The contraceptive action of glycerol in chickens. Poult Sci 50:1411-1415.   DOI
15 Polge C, Smith AU, Parkes AS 1949 Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature 164:666.   DOI
16 Sasaki K, Tatsumi T, Tsutsui M, Tatsuya N, Imai Y, Naito M, Tajima A, Nishi Y 2010 A method for cryopreserving semen from Yakido roosters using N-methylacetamide as a cryoprotective agent. J Poult Sci 47:297-301.   DOI
17 Sexton TJ 1981 Sperm number required for maximum fertility of chicken semen processed for freezing. Reprod Nutr Dev21:1043-1048.   DOI
18 Sexton TJ, Fewlass TA 1978 A new poultry semen extender. Effeect of the diluent components on the fertility of chicken semen stored at $5^{\circ}C$. Poult Sci 57: 277-284.   DOI
19 Shaffner CS, Henderson EW, Card GC 1941 Viability of spermatozoa of the chicken under various environmental conditions. Poult Sci 20:259-265.   DOI
20 White LM, Beal WE, Bame JH, Saacke RG, Marshall CE 1984 Characteristics of bovine spermatozoa after migration through a bovine serum albumin gradient. J Anim Sci 59: 454-459.   DOI
21 Vantman D, Koukoulis G, Dennison L, Zinaman M, Sherins RJ 1998 Computer-assisted semen analysis: Evaluation of method and assessment of the influence of sperm concentration on linear velocity determination. Fertil Steril 49: 510-515.