• Journal of Periodontal and Implant Science
• /
• v.32 no.3
• /
• pp.685-695
• /
• 2002

황색 포도상구균은 급성 구강 감염에 있어서의 병원균이다. 그러나 그러한 황색 포도상구균의 병원성 기전은 완전히 이해되지 않았다. 이전 실험에서 황색 포도상구균의 단백질 A와 골격근의 액틴 필라멘트는 인체 상피 세포로의 황색 포도상구균의 침입에 관여한다. 구강 내 감염에 있어서의 황색 포도상구균의 병원성 기전을 조사하기 위해 인체의 치은 섬유모 세포에 대한 침입이 연구되고 있다. 급성 구강 감염을 가진 환자로부터 분리된 ATCC 25923 황색 포도상구균과 OPT 2 황색 포도상구균의 침입은 시간(0-120분)에 의존한다는 사실을 밝혀냈다. 60분을 초과하는 배양시간은 다시 배양된 균집락수 증가를 가져왔다. 배지에 접종한 세균의 숫자가 증가할 때 (100?10,000,000 cfu/ml/well), 직선적으로 증가한다. 단백질 A가 결핍된 Wood 46 황색 포도상구균의 침입은 단백질 A가 발현된 균주(ATCC 25923과 OPT 2)의 침입보다 훨씬 낮았다. 액틴 필라멘트의 합성을 방해하는 Cytochalasin D는 인체 치은 섬유모세포로 황색 포도상구균 (ATCC 25923과 OPT 2)이 침입하는 것을 방해한다. 이러한 결과는 구강내 감염을 일으키는 황색 포도상구균의 병원성 기전이 세포내 침입에 관여하고, 황색 포도상구균 단백질 A와 골격근의 액틴 필라멘트가 인체 치은 섬유모세포로의 황색 포도상구균의 침입 조절에 관여한다는 것을 보여준다.

• Proceedings of the Korean Aquaculture Society Conference
• /
• 2003.10a
• /
• pp.82-83
• /
• 2003

최근 인공종묘생산기술이 개발된 붉은쏨뱅이(Sabastiscus tertius)는 자연산과 양식산의 체색이 붉은색과 검정색으로 많은 차이가 있어 양식산업화에 많은 어려움이 있다. 따라서 상품가치와 경쟁력 향상을 위하여 사료에 카로티노이드 성분을 함유한 크릴새우(25, 50, 75, 100%)와 astaxanthin(5, 10, 15, 20%)를 비율대로 첨가하여 양식산 붉은쏨뱅이의 체색 변화를 살펴보았다. 크릴새우의 경우 50% 첨가한 사료실험구에서의 적색도와 황색도는 각각 6.29와 13.42로 높게 나타나기 시작하였으며 크릴새우 75% 첨가실험구에서는 적색도와 황색도가 각각 8.31과 14.91로 최고치를 보였다. 그리고 크릴 100%에서는 적색도와 황색도가 각각 7.27과 10.33로 75% 실험구에 비하여 감소하는 것으로 나타났다. astaxanthin 실험구들에서는 5%와 10% 실험구에는 체색도가 높게 나타나지 않았으나 15%에서 적색도가 7.86 황색도가 9.32로 가장 높게 나타났다. 그리고 20% 실험구에는 7.18과 6.32로 나타났다. 본 실험결과 붉은쏨뱅이의 체색회복을 위해서는 크릴새우의 경우 최소 50% 이상을 첨가해야만 가능할것으로 판단되며 astaxanthin는 15% 이상 첨가해야 효과가 있는 것으로 판단된다. 그리고 astaxanthin보다는 크릴새우가 더 효과적이였으며 모든 실험구는 대조구(펠렛사료 100%)에 비하여 체색 회복효과가 있는 것으로 나타났다. 또한 자연산에서는 적색도와 황색도는 각각 23.80과 13.44로 적색도가 아주 높게 나타났으나 양식산에서는 적색도와 황색도가 1.09와 1.98로 매우 낮게 나타났다. 그리고 체색의 변화는 모든실험구에서 등지느러미와 꼬리지느리미 그리고 턱과 가슴주변에서부터 점차 배와 등쪽으로 색이 변화하는 것이 관찰되었다. 크릴 75%와 astaxanthin 15%를 공급하였을 때 체색이 변화하는 시간은 크릴의 경우 사료공급 20일 후부터 체색의 변화가 시작하여 30일이 경과 될때에는 등지느러미, 꼬리지느러미 및 턱주변이 붉은색으로 변화하였다. astaxanthin의 경우는 30일부터 등지느러미와 꼬리지느러미 부근에서 체색의 변화가 시작되었으며 40일 이후부터 붉은색으로 변화하였다. 크릴새우와 astaxanthin를 함유하여 실험하였을 때 붉은쏨뱅이의 성장은 크릴 75% 실험구에서 전장 172.3mm, 체중 65.9g으로 가장 좋았다. 그러나 대조구에서는 전장 148.7mm, 체중 49.5g으로 가장 성장이 저조하였으며 astaxanthin 실험구들에서는 전장 152.3~159.8mm, 체중 56.9~59.3g으로 서로 유의적인 차이를 나타내지 않았다.

• Microbiology and Biotechnology Letters
• /
• v.8 no.3
• /
• pp.167-172
• /
• 1980

Yellow pigments were extracted with mixture of 60% -ethanol and petroleum ether (1 : 2) by method of partition chromatography in petroleum ether phase. The absorption curve of yellow pigments solution exhibits maximum peak at wave length range of 394-403um. By thin layer chromatography yellow pigments preparation were found to consists of monascin(yellow), monascidin A (pale yellow) and one unknown (orange-yellow) compound. Isolated fat soluble yellow pigments were changed to water soluble by N-KOH (adjust pH 9). The resulted product obtained were yellow pigments of K-salt complex.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.20 no.1
• /
• pp.17-22
• /
• 1984

The author carried out an experiment to find on the optomotor response of crusian carp, Carassius carassius and goldfish, Carassius auratus in accordance with the colors of the wall and the stripes labeled on the revolving visual screen. The results are as follows; 1. The optomotor response rate of crusian carp was 80% or more in case of B-Y, R-Bl and W-Bl pair of colors, and 50% or less in case of Bl-B, B-Bl, R-W, R-Y, W-Y and Y-W pair. 2. The optomotor response rate of goldfish was 80% or more in all except B-Bl, W-R and W-Y pair. 3. Mean round number of crusian carp was 7.5-9.5 revolutions in case of W-Bl, Y-Bl, W-B and Y-B pair, and 1.8-3.9 revolutions in case of Bl-B, Bl-Y, B-Bl, B-R, R-All and Y-W pair for 3 minutes. 4. Mean rounded number of goldfish was only 4.1-4.9 revolutions in case of W-Bl, W-B and Y-B pair for 3 minutes.

• Journal of Life Science
• /
• v.3 no.3
• /
• pp.143-151
• /
• 1993

녹황색 채소류는 암을 예방하는 대표적인 식품으로 인정되고 있다. 영양학적으로 볼 때 녹황색 채소류는 비타민 및 무기질의 주요 급원이 되고, 맛에 영향을 주는 당류 및 아미노산과 또한 많은 양의 식이 섬유소로 이루어져 있다. 특히 beta-카로틴과 비타민 C가 풍부하여 영양급원으로서 뿐만 아니라 생체내 생리활성에도 중요한 영향을 끼친다. 각종 암 발생과 관련하여서도 예방적인 역할을 하는 것으로 알려져 있는데 특히 폐암의 발생을 낮추며, 우리 나라와 일본에서 상당히 높은 사망원인이 되고 있는 위암 발생을 채소류의 섭취로 줄일 수 있다고 보고되고 있다. 이에 각종 암의 발생과 녹황색 채소의 섭취는 부의 상관관계를 가진다고 하겠다.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.31 no.2
• /
• pp.338-342
• /
• 2002

Antimicrobial activity of yellow pigment produced by Monascus anka Y7 (Y7) was studied. The crude yellow pigment of Y7 showed antimicrobial activity against some bacteria and yeasts. The diameter of inhibition zone against gram-positive bacteria was a little smaller t]fan that of gram-negative bacteria to the crude yellow pigment. Especially, E2 fraction obtained from the crude yellow pigment by TLC method showed high anti-microbial activity against E. coli.. The fraction had bright yellow pigment, showing fluorescent light and having the maximum absorption at 373 nm. Citrinin, a mycotoxin which had been characterized as an antimicrobial substance from a Monascus strain, was not detected in the E2 fraction and in the crude yellow pigment by the results of TLC and HPLC. This indicates that the antimicrobial activity of Y7 pigments did not any relationship with citrinin. Yellow degree (b/a of Hunters color value) of Y7 pigment was much higher than that of other natural colorants such as annatto, gardenia yellow and carthamus yellow. But the colors of all of the yellow pigments were similar by panels. Thus, the yellow pigment of Y7 could be used as a useful alternative colorant for food industry, having the advantage of antimicrobial activity.

• FLOWER RESEARCH JOURNAL
• /
• v.16 no.1
• /
• pp.76-84
• /
• 2008

This study was conducted to find out the cause of discoloration after pressing yellow-colored flowers. Plant materials used were Lilium Asiatic Hybrids, Oncidium spp., Rosa hybrida, $Tulipa{\times}gesneriana$, Antirrhinum majus, Dianthus caryophyllus, Freesia hybrida, Zantedeschia elliottiana, Gerbera jamesonii, Helianthus annuus, and Ranunculus asiaticus. Thickness, ratio of length to width, and water content of petals did not considerably affect discoloration of pressed flowers. Expression of yellow color in petals was caused by carotenoids in Lilium Asiatic Hybrids, Oncidium spp., Rosa hybrida and $Tulipa{\times}gesneriana$, by flavonoids in Antirrhinum majus, Dianthus caryophyllus, Freesia hybrida and Zantedeschia elliottiana, and by coexistence of carotenoids and flavonoids in Gerbera jamesonii, Helianthus annuus and Ranunculus asiaticus. Carotenoids that expressed yellow color in petals affected significantly on discoloration in pressed flowers containing carotenoids alone or both carotenoids and flavonoids. However, discoloration was hardly ever found in yellow-colored species as affected by flavonoids. Dark treatment was effective for almost perfect preservation in color of pressed flowers, while light treatment resulted in severe discoloration.

• The Korean Journal of Food And Nutrition
• /
• v.10 no.2
• /
• pp.241-245
• /
• 1997

In order to make natural food color from Gardenia, we investigated optimal conditions of color extraction, and thermal stability and light stability of color extracted compared with Yellow-4. In case of ethanol extraction, optimal conditions for color extraction were substrate 10%, 4$0^{\circ}C$, pH 7.0 and 42rs, respectively. In case of water extraction, optimal conditions for color extraction were substrate 10%, 7$0^{\circ}C$, pH 7.0 and 48hrs, respectively. Extraction yield in the optimal conditions was 75% in ethanol and 63% in water. The thermal stability and light stability of Yellow-4 were both upper 98%, but those of Gardenia yellow color were 62 and 90%, respectively.

• Tuberculosis and Respiratory Diseases
• /
• v.64 no.1
• /
• pp.22-27
• /
• 2008

Background: Staphylococcus aureus frequently colonizes and infects hospitalized patients. Respiratory infections with Staphylococcus aureus are common in patients with compromised airway defenses. However the mechanisms of S. aureus invasion from colonization to the epithelium are unclear. Cell invasion by S. aureus would require destruction of the extracellular matrix, which is believed to be the result of increased matrix metalloproteinases (MMP) activity. Methods: In this study, respiratory epithelial cells were infected with S. aureus. After removing the extracellular bacteria by washing, the internalized bacteria in the cells were assessed by counting the colonized forming units (CFUs). The cell adhesion proteins, dysadherin and E-cadherin, were evaluated by Western blotting. The MMPs in the bacterial invasion were evaluated by pretreating the cells with GM6001, a MMP inhibitor. Results: The internalization of S. aureus was found to be both time and dose dependent, and the increase in MMP 2 and 9 activity was also dependent on the incubation time and the initial amount of bacterial inoculation. The invasion of S. aureus was attenuated by GM6001 after 12 hours incubation with a multiply of infection (MOI)=50. The expression of dysadherin, a membrane protein, was increased in a time and dose dependent manner, while the expression of E-cadherin was decreased. Conclusion: MMPs may mediate the invasion of S. aureus into epithelial cells.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.50 no.spc1
• /
• pp.141-146
• /
• 2005

The objective of this study was to determine the characterisics of quality and antioxidant with various sweet potato(SP) varieties. The differences of skin color between general and colored SP was not significant. The flesh color of steamed SP was the lower than flesh of raw SP, and especially steamed purple SP(5.24) was lower than flesh SP(22.0). In physical characteristics, the hardness of raw SP showed the low values and the springness, gumness and hardness among steamed SP varieties was not significant. The total phenol content(TPC) of purple, yellow-fleshed and general SP had 13.1, 6.22 and 3.02 mg/g, respectively. In DPPH, the purple-fleshed SP had the highest free radical scanvering. Also, shingunmi$(88.5\%)$, Saengmi$(80.9\%)$ and Shinchunmi$(78.9\%)$ show the high antioxidant activities. The redness of SP was positively correlated with TPC in raw and steamed. However, the yellowness of flesh SP was negatively correlated with DPPH.