• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.24-24
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• 2015

최근 원격탐사기법을 이용한 많은 가뭄평가기법들이 개발되었으나 산림과 함께 산악지형이 우세한 우리나라의 경우 지형특성으로 인하여 가뭄평가시 불확실성이 증가하게 된다. 특히, 농업가뭄은 기후와 지표특성에 큰 영향을 받기 때문에 기후특성만을 고려한 가뭄지수는 실제 필요한 농업가뭄의 특성을 반영하는데 있어서 한계가 있다. 따라서 본 연구에서는 기후와 지표특성을 함께 고려할 수 있는 토양수분을 이용한 가뭄평가기법(Drought Assessment Scheme)을 개발하였다. 가뭄평가기법을 위하여 역추적기법(Inverse Modeling-IM) 기반의 자료동화기법(Data Assimilation, DA)을 이용하였다. 자료동화기법은 1-Dimensional (1-D) 기반의 토양의 물리적 특성을 고려하는 SWMI_ST 모형과 최적화 알고리즘(유전자 알고리즘, Genetic Algorithm-GA)을 연계하여 실측 및 위성기반의 토양수분자료로부터 토양의 수리학적 매개변수(${\alpha}$, n, ${\Theta}_{res}$, ${\Theta}_{sat}$, $K_{sat}$)를 추출한다. 본 연구에서는 LANDSAT(30 m X 30 m) 및 MODerate Resolution Imaging Spectroradiometer(MODIS, 500 m X 500 m) 이미지자료를 이용하여 시 공간적으로 분포되어 있는 토양수분을 산정하였으며, 이후 자료동화기법을 이용하여 LANDSAT/MODIS 토양수분자료로 부터 공간적으로 분포되어 있는 토양의 매개변수를 추출하였다. 추출된 매개변수, GIS 기반의 지표피복 및 기상자료를 이용하여 장기간의 토양수분을 산정 및 예측 할 수 있다. 고해상도의 이미지 자료를 사용하는 가뭄평가기법은 필지~시 군 단위까지 실제 우리나라 지형특성을 고려하여 효율적으로 가뭄을 모니터링 및 예측 할 수 있다.

• Membrane Journal
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• v.34 no.2
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• pp.96-104
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• 2024

Antibiotics is one of the emerging pollutants found in various water sources as well as wastewater due to its excessive use. Different techniques are available for treating antibiotics contaminants in water such as advanced oxidation process and biological treatment etc. These two processes are ineffective, and the generation of side products makes this process more complicated. Membrane technology is another alternative for the removal of contaminants. To improve the removal of antibiotics and their resistant gene, membrane bioreactors are modified with NaClO and carbon materials. The generation of abundant reactive species is active against the antibiotic's resistant genes.

• Journal of KIBIM
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• v.13 no.4
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• pp.85-95
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• 2023

In the process of creating drawings based on Building Information Modeling (BIM), automatically generated annotations can cause interference issues depending on the drawing type. This study aims to develop an algorithm for repositioning annotations using genetic algorithms to minimize such interferences. To achieve this, the Application Programming Interface (API) of BIM software was used to analyze data extractable from BIM drawing files. The process involved defining drawing data related to annotation repositioning, preprocessing this data, and deriving optimal placement coordinates for the annotations. Furthermore, applying the developed algorithm to the preliminary design drawings of small and medium-sized neighborhood facilities resulted in approximately a 95.37% decrease in annotation interference, indicating that the proposed algorithm can significantly enhance productivity in BIM-based drawing tasks.

• Korean Journal of Environmental Biology
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• v.29 no.3
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• pp.138-143
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• 2011

The efficiency of mtCOI amplication after DNA extraction of benthic harpacticoid Tigriopus japonicus s.l. was tested under different conditions depending on fixative (99% Ethanol, or 4% Formalin) and additional chemicals (Ludox or Rose Bengal). Each experimental group by the fixative was subdivided into four groups, respectively: 1) Control (fixative only), 2) processed with Ludox HS40, 3) processed with Rose Bengal, and 4) processed with both Ludox HS40 and Rose Bengal. For the 99% ethanol-fixed sample, overall success rate of amplification by PCR was 96% or above, while for the 4% formalin-fixed one, success rate was much lower than those of ethanol-fixed: 1) Control: 27%, 2) Ludox HS40: 3%, 3) Rose Bengal: 7%, and 4) Ludox HS40 and Rose Bengal: 3%. As a result present study verify that 99% ethanol is a proper fixative for DNA extraction in meiofauna organisms.

• Korean Journal of Environmental Biology
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• v.24 no.2 s.62
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• pp.112-118
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• 2006

We report mitochondrial cytochrome b (cyt b) genes from greenling Hexagrammos otakii (Jordan et Starks) and spotty belly greenling, H. agrammus (Temminck et Schlegel) within Hexagrammidae, two species of aquaculture importance. Of 489 bp of the cytochrome b gene, a little variation occurred between species (96% similarity). The pairwise distance (0.0342) between greenling and spotty belly greenling in term of the Neighbor-joining method indicated that two species was close in molecular phylogenetic consideration. These findings are applicable to aquaculture, fisheries genetics and molecular phylogenetics in the genus Hexagrammos.

• Korean Journal of Environmental Biology
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• v.21 no.1
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• pp.45-51
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• 2003

Genetic diversity of local populations among geologically isolated groups of Rana amurensis was refined by sequence comparison of the mitochondrial (mt) 165 rDNA genes. Each 401 base pairs of DNA sequences, which was determined from four local populations of Rana amurensis, two local populations of R. nigromacutata, and three species of the genus Rana were used in this analysis. Despite morphological similarity of Rana amurensis, Korean populations were well distinguished from the other groups on the basis of 105 rDNA gene difference. Further analyses for additional local populations belonging to R. amurensis will be necessary to clarify the taxonomic status.

• Food Industry And Nutrition
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• v.11 no.3
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• pp.6-11
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• 2006

바이러스성 식중독은 장염을 일으키는 원인 병원체 중 노로바이러스에 의해 흔히 발생하며 이외에도 아스트로바이러스나 로타바이러스에 의한 집단 설사 사례가 국내에서 보고된 바 있다. 노로바이러스는 식중독과 관련하여 특히 오염된 식수와 굴 등 어패류의 생식을 통한 감염 사례가 많이 보고되어 있으나 사람 간 전파도 흔히 일어나는 전염력이 매우 높은 바이러스이다. 국내에서는 1999년 이후 보고가 되고 있으며 최근 집단 급식과 관련된 대형 식중독 사례들이 보고되면서 학교급식이 사회적인 이슈로 대두되고 있다. 2000년 이후 질병관리본부는 바이러스성 설사의 국내 발생현황을 파악하기 위하여 전국의 17개 시도보건환경 연구원과 노로바이러스를 포함한 4종의 바이러스성 장염원인 병원체에 대한 전국적인 실험실 감시체계를 운영한 결과 바이러스성 병원체가 확인된 사례의 약 18%에서 노로바이러스가 검출되었고, 집단설사 사례에서는 대부분 노로바이러스가 원인병원체로 확인되었다. 또한 노로바이러스의 조기 검출을 위해 질병관리본부는 2004년 중 노로바이러스 유전자 검출 kit를 자체적으로 제작하여 이를 전국의 시도 보건환경연구원을 연계한 감시체계에서 적극 활용함으로써 노로바이러스 집단설사사례의 조기 검출이 가능하게 되었고 지역내 노로바이러스 검출율을 높이는데 기여하였다. 국립보건연구원은 2003년과 2006년에 발생한 대규모 노로바이러스 식중독 사례 이외에도 산발적으로 지속적으로 발생하는 사례들을 조기에 탐지하고 국내에서 검출되는 설사바이러스 유전형 분포양상과 새로운 유전자형이나 변이주를 조기에 검출하고자 전국적인 노로바이러스 실험실 감시망을 강화하여 운영하고 있으며, 집단설사 발생시 각 사례의 연관성을 신속하게 분석할 수 있는 실시간 분자역학적 유전자 분석체계를 단계적으로 도입하고 있다. 실험실 감시체계 운영과 함께 집단 식중독 유발 병원체의 효율적인 관리를 위해 질병관리본부는 노로바이러스를 포함한 설사 유발 병원체를 신고대상 병원체로 지정(2006.06.12)하여 병원체 검출시 보고하도록 하고 관련 지침을 마련하였다. 노로바이러스가 지정전염병 병원체로 추가로 지정됨에 따라 집단 사례 및 실험실 감시사업을 통해 검출되는 병원체에 대한 보고가 강화되고 전파 방지와 2차 감염 사례 감소에도 기여할 수 있을 것으로 사료되며 전국의 실험실 감시망을 연결하는 국가 차원의 노로바이러스 실시간 분자역학적 분석체계 도입을 통해 노로바이러스 2차 감염을 줄이고 대규모 집단발병 및 유행의 조기 차단 효과를 가져올 수 있을 것이다.

• The Journal of the Acoustical Society of Korea
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• v.18 no.8
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• pp.32-41
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• 1999

In this paper, an inversion method using chirp signals and two near field receivers is proposed. Inversion problems can be formulated into the probabilistic models composed of signals, a forward model and noise. Forward model to simulate chirp signals is chosen to be the source-wavelet-convolution planewave modeling method. The solution of the inversion problem is defined by a posteriori pdf. The wavelet matching technique, using weighted least-squares fitting, estimates the sediment sound-speed and thickness on which determination of the ranges for a priori uniform distribution is based. The genetic algorithm can be applied to a global optimization problem to find a maximum a posteriori solution for determined a priori search space. Here the object function is defined by an L₂norm of the difference between measured and modeled signals. The observed signals can be separated into a set of two signals reflected from the upper and lower boundaries of a sediment. The separation of signals and successive applications of the genetic algorithm optimization process reduce the search space, therefore improving the inversion results. Not only the marginal pdf but also the statistics are calculated by numerical evaluation of integrals using the samples selected during importance sampling process of the genetic algorithm. The examples applied here show that, for synthetic data with noise, it is possible to carry out an inversion for sedimentary layers using the proposed inversion method.

• Journal of Plant Biotechnology
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• v.31 no.2
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• pp.109-113
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• 2004

In order to develop transgenic potato plants with enhanced tolerance to multiple stress, we constructed the transformation vector expressing both superoxide dismutase and ascorbate peroxidase genes in chloroplasts under the control of a stress-inducible SWPA2 promoter. Transgenic potato plants (cv. Superior and Atlantic) were generated using an Agrobacterium-mediated transformation system. Transgenic potato plants were regenerated on MS medium containing 100mg/L kanamycin. Genomic Southern blot analysis confirmed the incorporation of foreign genes into the potato genome. When potato leaf discs were subjected to methyl viologen (MV) at 10 $\mu$M, transgenic plants showed higher tolerance than non-transgenic or vector-transformed plants. To further study we selected the transgenic plant lines with enhanced tolerance against MV. These plants will be used for further analysis of stress-tolerance to multiple environmental stresses.

• Korean Journal of Environmental Biology
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• v.37 no.4
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• pp.585-591
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• 2019

Most insect-resistant LMOs have been produced by applying Cry and Vip3Aa proteins. Vip3Aa protein is activated during the vegetative stage of Bacillus thuringensis (Bt) and the inhibitory activity of the Vip3Aa protein against pathogenic attacks from lepidopteran insect species is well known. However, a risk assessment of the Vip3Aa protein compared to the Cry protein has not been conducted in South Korea. This study demonstrates a possible risk assessment method for Vip3Aa protein against honeybees (Apis mellifera). For the risk assessment of the protein, we purified the recombinant Vip3Aa protein in Escherichia coli. The survival rate and symptoms of general intoxication of 4 months honeybees were measured after Vip3Aa exposure. These results indicated that there was no significant difference in the survival rate and the symptom between Vip3Aa and the control buffer. In this study, we established standard methods of Vip3Aa protein purification and oral adult toxicity test using A. mellifera as an LMO risk assessment technique for preserving the natural ecosystem of South Korea.