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Comparative Study of DNA Extraction Method in Meiofauna  

Lee, Seung-Han (Department of Life Science, College of Natural Sciences, Hanyang University)
Back, Jin-Wook (Department of Life Science, College of Natural Sciences, Hanyang University)
Lee, Won-Choel (Department of Life Science, College of Natural Sciences, Hanyang University)
Publication Information
Korean Journal of Environmental Biology / v.29, no.3, 2011 , pp. 138-143 More about this Journal
Abstract
The efficiency of mtCOI amplication after DNA extraction of benthic harpacticoid Tigriopus japonicus s.l. was tested under different conditions depending on fixative (99% Ethanol, or 4% Formalin) and additional chemicals (Ludox or Rose Bengal). Each experimental group by the fixative was subdivided into four groups, respectively: 1) Control (fixative only), 2) processed with Ludox HS40, 3) processed with Rose Bengal, and 4) processed with both Ludox HS40 and Rose Bengal. For the 99% ethanol-fixed sample, overall success rate of amplification by PCR was 96% or above, while for the 4% formalin-fixed one, success rate was much lower than those of ethanol-fixed: 1) Control: 27%, 2) Ludox HS40: 3%, 3) Rose Bengal: 7%, and 4) Ludox HS40 and Rose Bengal: 3%. As a result present study verify that 99% ethanol is a proper fixative for DNA extraction in meiofauna organisms.
Keywords
meiofauna; DNA extraction; mtCOI; Ludox; Rose Bengal;
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