Browse > Article
http://dx.doi.org/10.11626/KJEB.2019.37.4.585

Purification and risk assessment of Bacillus thuringiensis Vip3Aa protein against Apis mellifera  

Jung, Young Jun (National Institute of Ecology)
Yoo, Su-Hyang (National Institute of Ecology)
Lee, Jung Ro (National Institute of Ecology)
Publication Information
Korean Journal of Environmental Biology / v.37, no.4, 2019 , pp. 585-591 More about this Journal
Abstract
Most insect-resistant LMOs have been produced by applying Cry and Vip3Aa proteins. Vip3Aa protein is activated during the vegetative stage of Bacillus thuringensis (Bt) and the inhibitory activity of the Vip3Aa protein against pathogenic attacks from lepidopteran insect species is well known. However, a risk assessment of the Vip3Aa protein compared to the Cry protein has not been conducted in South Korea. This study demonstrates a possible risk assessment method for Vip3Aa protein against honeybees (Apis mellifera). For the risk assessment of the protein, we purified the recombinant Vip3Aa protein in Escherichia coli. The survival rate and symptoms of general intoxication of 4 months honeybees were measured after Vip3Aa exposure. These results indicated that there was no significant difference in the survival rate and the symptom between Vip3Aa and the control buffer. In this study, we established standard methods of Vip3Aa protein purification and oral adult toxicity test using A. mellifera as an LMO risk assessment technique for preserving the natural ecosystem of South Korea.
Keywords
Bacillus thuringiensis; Vip3Aa; insecticidal protein; Apis mellifera; risk assessment;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Ali MI and RG Luttrell. 2011. Susceptibility of Helicoverpa zea and Heliothis virescens (Lepidoptera: Noctuidae) to Vip3A insecticidal protein expressed in VipCotTM cotton. J. Invertebr. Pathol. 108:76-84.   DOI
2 Baek HJ, SI Sohn, MR Cho, GS Lee, YJ Oh, JS Park, KJ Lee, SD Oh, SC Suh and TH Ryu. 2010. Development of protocol for analyzing pollinator insect-mediated gene transfer from gm crop. Korean J. Int. Agric. 22:293-297.
3 Bergamasco VB, DR Mendes, OA Fernandes, JA Desiderio and MV Lemos. 2013. Bacillus thuringiensis Cry1Ia10 and Vip3Aa protein interactions and their toxicity in Spodoptera spp. (Lepidoptera). J. Invertebr. Pathol. 112:152-158.   DOI
4 Bravo A, SS Gill and M Soberon. 2007. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon 49:42-435.
5 CERA. 2012. A Review of the Environmental Safety of Vip3Aa. Center for Environmental Risk Assessment. International Service for the Acquisition of Agri-biothch Applications (ISAAA). http://isaaa.org.
6 Chakroun M, N Banyuls, Y Bel, B Escriche and J Ferre. 2016. Bacterial vegetative insecticidal proteins (Vip) from entomopathogenic. Microbiol. Mol. Biol. Rev. 80:329-350.   DOI
7 Donovan WP, JC Donovan and JT Engleman. 2001. Gene knockout demonstrates that vip3a contributes to the pathogenesis of Bacillus thuringiensis toward Agrotis ipsilon and Spodoptera exigua. J. Invertebr. Pathol. 78:45-51.   DOI
8 Donovan WP, JT Engleman, JC Donovan, JA Baum, GJ Bunkers, DJ Chi, WP Clinton, L English, GR Heck, OM Ilagan, KC Krasomil-Osterfeld, JW Pitkin, JK Roberts and MR Walters. 2006. Discovery and characterization of Sip1A: A novel secreted protein from Bacillus thuringiensis with activity against coleopteran larvae. Appl. Microbiol. Biotechnol. 72:713-719.   DOI
9 Estruch JJ, GW Warren, MA Mullins, GJ Nye, JA Craig and MG Koziel. 1996. Vip3A, a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects. Proc. Natl. Acad. Sci. USA 93:5389-5394.   DOI
10 Hilbeck A. 2001. Implications of transgenic, insecticidal plants for insect and plant biodiversity. Perspect. Plant Ecol. Evol. Syst. 4:43-61.   DOI
11 Hutchison WD, EC Burkness, PD Mitchell, RD Moon, TW Leslie, SJ Fleischer, M Abrahamson, KL Hamilton, KL Steffey, ME Gray, RL Hellmich, LV Kaster, TE Hunt, RJ Wright, K Pecinovsky, TL Rabaey, BR Flood and ES Raun. 2010. Area wide suppression of European corn borer with Bt maize reaps savings to non-Bt maize growers. Science 330:222-225.   DOI
12 James C. 2015. 20th anniversary of the global commercialization of biotech crops (1996 to 2015) and biotech crop highlights in 2015. ISAAA Brief 51:2-3.
13 Kim BS, YJ Yang, YK Park, MH Jeong, AS You, KH Park and YJ Ahn. 2009. Risk assessment of fipronil on honeybee (Apis mellifera). Korean J. Pestic. Sci. 13:39-44.
14 Lee B, CG Kim, JY Park, H Yi, KW Park, WK Jeong, JH An, KH Cho and HM Kim. 2007. Survay of herbicide resistant oilseed rapes around the basin of rivers in Incheon harbor area. Korean J. Weed Sci. 27:29-35.
15 Lee B, JH Kim, SI Sohn, SJ Kweon, KW Park, YS Chung and SM Lee. 2015. Influence of insect pollinators on gene transfer from GM to nonGM soybeans. Korean J. Agric. Sci. 42:159-165.   DOI
16 Lemes ARN, CS Figueiredo, I Sebastiao, L Marques da Silva, R da Costa Alves, HAA de Siqueira, MVF Lemos, OA Fernandes and JA Desiderio. 2017. Cry1Ac and Vip3Aa proteins from Bacillus thuringiensis targeting Cry toxin resistance in Diatraea flavipennella and Elasmopalpus lignosellus from sugarcane. PeerJ 5:2866.
17 Levine TJ, SL Bachman, PM Jenson, PD Mueller, GM Uffman, JP Meng, C Song, ZK B Richards and MH Beevers. 2015. No impact of DvSnf7 RNA on honey bee (Apis mellifera L.) adults and larvae in dietary feeding tests. Environ. Toxicol. Chem. 35:287-294.   DOI
18 Lim HS, YJ Jung, IR Kim, J Kim, S Ryu, B Kim, JR Lee and W Choi. 2017. Acute oral toxicity of dsRNA to honeybee, Apis mellifera. Korean J. Environ. Agric. 36:241-248.   DOI
19 Milne R, Y Liu, D Gauthier and K Frankenhuyzen. 2008. Purification of Vip3Aa from Bacillus thuringiensis HD-1 and its contribution to toxicity of HD-1 to spruce budworm (Choristoneura fumiferana) and gypsy moth (Lymantria dispar) (Lepidoptera). J. Invertebr. Pathol. 99:166-172.   DOI
20 Palma L, D Munoz, C Berry, J Murillo and P Caballero. 2014. Bacillus thuringiensis toxins: An overview of their biocidal activity. Toxins 6:3296-3325.   DOI
21 Park KW, CK Kim, R Lee, DY Kim, JY Park, DI Kim, MC Kwon, H Yi and HM Kim. 2007. Monitoring of imported genetically modified crops in the cultivated fields in Korea. Korean J. Weed Sci. 27:318-324.
22 Park SC, IR Kim, JE Hwang, JY Kim, YJ Jung, W Choi, Y Lee, MK Jang and JR Lee. 2019. Functional mechanisms underlying the antimicrobial activity of the Oryza sativa Trx-like protein. Int. J. Mol. Sci. 20:1413.   DOI
23 Park T, H Choe, H Jeong, H Jang, J Kim and JJ Park. 2018. Comparison of insect fauna in transgenic and common rice paddy fields. Korean J. Environ. Biol. 36:488-497.   DOI
24 Patricia HM, SHR Carmen, VR Jeroen, E Baltasar and F Juan. 2013. Insecticidal activity of Vip3Aa, Vip3Ad, Vip3Ae, and Vip3Af from Bacillus thuringiensis against lepidopteran corn pests. J. Invertebr. Pathol. 113:78-81.   DOI
25 Quist D and IH Chapela. 2001. Transgenic DNA introgressed into traditional maize landraces in Oaxaca, Mexico. Nature 414: 541-543.   DOI
26 Romeis J, D Bartsch, F Bigler, MP Candolfi, MMC Gielkens, SE Hartley, RL Hellmich, JE Huesing, PC Jepson, R Layton, H Quemada, A Raybould, RI Rose, J Schiemann, MK Sears, AM Shelton, J Sweet, Z Vaituzis and JD Wolt. 2008. Assessment of risk of insect-resistant transgenic crops to nontarget arthropods. Nat. Biotechnol. 26:203-208.   DOI
27 Schnepf E, NV Crickmore, J van Rie, D Lereclus, J Baum, J Feitelson, DR Zeigler and DH Dean. 1988. Bacillus thuringiensis and its pesticidal Crystal proteins. Microbiol. Mol. Biol. Rev. 62:775-806.   DOI
28 Warren GW, MG Koziel, MA Mullins, GJ Nye, B Carr, NM Desai, K Kostichka, NB Duck and JJ Estruch. 1998. Auxiliary proteins for enhancing the insecticidal activity of pesticidal proteins. U.S. Patent No. 5,770,696. US Patent and Trademark Office. Washington, DC.