환경생물 (Korean Journal of Environmental Biology)

  • 제21권1호
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  • Pages.45-51
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  • 2003
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  • 1226-9999(pISSN)
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  • 2287-7851(eISSN)
한국환경생물학회 (Korean Society of Environmental Biology)
권호 표지

미토콘드리아 16S rDNA를 이용한 아무르산개구리 (양서 강: 개구리 과)의 유전적 다양성

Genetic Diversity of Rana amurensis (Amphibia: Ranidae), Based on Mitochondrial 165 rDNA Gene Sequences

  • 발행 : 2003.03.01
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초록

지리적으로 격리되어 있는 아무르산개구리 (Rana amurensis)의 유전적인 변이를 알아보기 위하여 미토콘드리아 165 rDNA 유전자 중 401 bp 염기서열을 분석하여 비교하였다. 아무르산개구리(4개 지역집단; 한국, 중국, 몽골 및 러시아), 참개구리(2개 지역집단: 한국, 일본) 및 다른 종류의 산개구리류 미토콘드리아 165 rDNA 유전자도 함께 비교하였다. 아무르산개구리의 형태적 유사성에도 불구하고, 한국 집단은 다른 지역의 집단들과 비교하여 염기서열상의 상당한 차이를 나타났다. 본 연구에서 염기서열 분석(401 bp 분석)으로 한국산 아무르산개구리가 아종인가 아니면 독립종인지에 대하여 설명할 수는 없으나, 본 연구의 결과와 Lee et at. (1999)에 의해 연구된 한국산 아무르산개구리의 미토콘드리아 cytochrome b유전자 분석 결과가 서로 일치하는 것을 알 수 있었다. 따라서, 한국산 아무르산개구리에 대한 분류학적 위치를 종 수준에서 다시 재검토해야 할 것으로 판단되나, 보다 명확한 결과를 위해서 더 많은 지역의 개체군을 포함하여 형태학적, 생태학적 연구가 진행될 필요가 있으며, 한국산 아무르산개구리의 유전적 차이도 함께 고려해 야 할 것으로 판단된다.

Genetic diversity of local populations among geologically isolated groups of Rana amurensis was refined by sequence comparison of the mitochondrial (mt) 165 rDNA genes. Each 401 base pairs of DNA sequences, which was determined from four local populations of Rana amurensis, two local populations of R. nigromacutata, and three species of the genus Rana were used in this analysis. Despite morphological similarity of Rana amurensis, Korean populations were well distinguished from the other groups on the basis of 105 rDNA gene difference. Further analyses for additional local populations belonging to R. amurensis will be necessary to clarify the taxonomic status.

키워드

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