• Title/Summary/Keyword: 혼용처리

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In Vitro Shoot Multiplication of Albizzia julibrissin Duraz. (자귀나무(Albizzia julibrissin Duraz.)의 기내 대량증식)

  • Ahn, Ji-Young;Kim, Jung-Hee;Kang, Ho-Duck
    • Korean Journal of Plant Resources
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    • v.19 no.4
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    • pp.524-529
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    • 2006
  • In vitro culture system was established to induce multiple shoots of Albizzia julibrissin Duraz. by investigating the effects of cytokinins. Cotyledon, hypocotyl and root explants were cultured on MS media supplemented with either three different plant growth regulators or their combinations. The most effective cytokinin sources were zeatin 2.0 + TDZ 0.5 mg/L in cotyledon, zeatin 1.0 mg/L in hypocotyl, and BA 0.2 + TDZ 0.01 mg/L in root explant for producing shoots ($5.67\;{\pm}\;1.20$, $19.50\;{\pm}\;3.50$, and $20.50\;{\pm}\;2.47$, respectively). Also, zeatin treatment was tended to induce more shoots rather than the combinations of other cytokinins. In addition, the root induced in 1/2 MS medium without any plant growth regulators was longer and thicker than treatments of IBA, NAA, IAA and 2.4-D as auxins. Overall, the highest average percent of in vitro shoot formation was 73% from three different types of explants with treatment of zeatin (1.0mg/L).

Interaction of Brassinolide with Other Known Plant Growth Regulators (Brassinolide와 기존 식물생장조절제(植物生長調節劑)와의 상호작용(相互作用))

  • Choi, C.D.;Takematsu, T.;Takeuchi, Y.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.7 no.1
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    • pp.78-83
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    • 1987
  • This study was attempted to evaluate the combining effect of HBR (homobrassinolide) with the known growth regulators such as GA (gibberellic acid), BA(6-benzyl aminopurine), IAA (indole-3-acetic acid), B-9 (N-dimethylamino succinamic acid) and CCC (2-chloroethyl-trimethylammonium chloride) on the growth of radish hypocotyl. A single application of HBR increased hypocotyl growth as its rates increased from 0.1 to 1.0 ppm, showing a maximum increase at 1.0 ppm. GA and BA had no direct effects on hyopcotyl growth, but IAA showed some effect as its concentration increased. However, the mixed application of HBR with GA, BA and IAA increased the length of radish hypocotyl as the concentration of HBR became higher. The mixture of HBR with GA and BA showed antagonistic reaction on radish hypocotyl growth, but synergistic effect was shown in the higher rate mixture of HBR with IAA in the range of HBR at 0.03 to 0.30 ppm with IAA at 3.0 to 10.0 ppm, but antagonistic or additive response at the mixture of low rates. An increased growth of hypocotyl by HBR was ified by CCC, showing the strong antagonistic reaction, but B-9 was not able to ify HBR's effect on hypocotyl growth.

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Effects of cytokinins, GA, and IBA on in vitro propagation of Vitex negundo var. insica (좀목형 (Vitex negundo var. insica) 신초의 기내증식에 미치는 cytokinin, GA 및 IBA의 영향)

  • Han, Mu-Seok;Moon, Heung-Kyu;Park, So-Young;Kim, Yong-Wook;Son, Suk-Gu
    • Journal of agriculture & life science
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    • v.45 no.3
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    • pp.53-58
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    • 2011
  • To develop an efficient micropropagation technique for Vitex negundo var. insica, which is known as aromatic and medicinal tree, the effects of various plant growth regulators (PGRs) on in vitro shoot proliferation and rooting were evaluated using the newly-developed shoots of a 3-year-old tree. Multiple shoot induction was achieved effectively on WPM (woody plant medium) supplemented with 0.5-2.0 mg/L BA, and the highest shoot number (7.9/explant) was obtained at the concentration of 1.0 mg/L BA. Typically 1 or 2 superior shoots (about 3.4 cm) were induced on hormone-free WPM. Combined treatment of BA 2.0 + GA 0.5 mg/L appeared to effective on shoot proliferation and rooting. Plant growth regulators added in shoot proliferation medium had strong impact on subsequent rooting as well. Overall, shoots induced by BA treatment resulted in high rooting rates while the effect was reduced gradually by ascending BA levels. TDZ of low concentration also revealed a similar tendency as BA, but the rooting ability was strongly inhibited at the concentration of 0.5 mg/L, and rooting was never observed at the concentrations higher than 0.5 mg/L. Combined treatment of BA and IBA had positive influence in both shoot proliferation and rooting. These results suggest that Vitex negundo var. insica could be effectively micropropagated via axillary bud cultures.

Performance Evaluation for ABR Service Control Algorithm over ATM network (ATM망에서의 ABR 서비스 제어 알고리즘에 관한 성능평가)

  • 박희대;육동철;박승섭
    • Proceedings of the Korea Multimedia Society Conference
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    • 2000.04a
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    • pp.30-35
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    • 2000
  • 초고속통신의 실현과 다양한 멀티미디어정보의 QoS를 향상시키기 위한 ATM 방식은 TCP/IP 통신의 기본적인 전송 매카니즘으로 사용되고 있다. TCP가 ATM 망에서의 ABR서비스를 사용할 때 ABR의 율 기반 제어와 TCP 흐름제어사이에 상호작용이 없는 한, 이런 환경하에서 ATM망의 ABR 트래픽을 좀더 효율적으로 제어하기위해서는 EFCI-ER 스위치 방식의 상호혼용 운영이 불가피하다. 본 논문에서는 이러한 상호혼용을 위하여 EFCI-ER 스위치의 혼용망을 모델로 하여 여러 가지 ER 스위치 알고리즘을 이용하여 대역할당의 공정성 셀손실을 그리고 처리율을 구하였다. 시뮬레이션 결과로써 여러 가지 ER 스위치 알고리즘중에서 망의 형태에 영향을 덜 받으면서 VC(Virtual Channel) 에 공정하게 대역폭을 할당할 수 있는 하나의 알고리즘을 나타내었다.

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Preharvest Drop Control of 'Tsugaru' Apple with Combined Use of Aminoethoxyvinylglycine and Several Adhesive Agents (Aminoethoxyvinylglycine과 몇몇 전착제의 혼용처리에 의한 '쓰가루' 사과의 수확전 낙과방지)

  • Kang, Chung Kil;Chae, Yun Seok;Kim, Su-Jeong
    • Horticultural Science & Technology
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    • v.17 no.6
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    • pp.750-752
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    • 1999
  • This experiment was conducted to evaluate preharvest drop control in 'Tsugaru' apple with combined treatments of aminoethoxyvinylglycine (AVG) and several adhesive agents. AVG+Silwet L-77 at $75+1,000mg{\cdot}L^{-1}$ was most effective among the tested combinations. Little or no significant difference was observed among the different application times of AVG+Silwet L-77. AVG+Silwet L-77 significantly reduced preharvest drop while increasing fruit weight, fruit length, diameter and fruit firmness. All the tested combinations were generally effective in reducing preharvest drop as compared to dichlorprop and in maintaining better fruit quality.

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Micropropagation of Delphinium cv. Princess Caroline through Shoot Tip Culture (정단배양에 의한 Delphinium cv. Princess Caroline의 대량번식)

  • 한봉희;정향영;고재영
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.1
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    • pp.53-55
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    • 1997
  • The shoot tips of Delphinium cv. Princess Caroline were cultured on the MS medium supplemented with cytokinin and auxin alone or in combination. Among cytokinins, BA was most effective in shoot multiplication, adequqte concentrations being 1.0-5.0 mg/L. Shoot multiplication was very favorable on the media with 1.0-3.0 mg/L BA and 0.1-0.5 mg/L IAA. Additions of BA and IAA did not stimulate shoot multiplication, but increased a little fresh weight. Shoots were scarcely rooted on the media with IBA or NAA, and were not done utterly on the media containing activated charcoal. Therefore, shoots were treated by Rootone and planted in the cultural media for in vivo rooting. The highest rate of rooting was 68% in the mixed cultural medium composed of Perlite 1 and Vermiculite 1.

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Plant Regeneration from Leaf Segments Culture of Several Jeju Native Lilies (잎절편 배양에 의한 제주 자생나리의 재분화)

  • Kim Jeong-Seon;Song Seung-Woon;Kim Yong Chol;Kim Kwang-Ho;Park Young-Chul
    • Korean Journal of Plant Resources
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    • v.18 no.3
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    • pp.450-455
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    • 2005
  • This study was conducted to examine the effects of plant growth regulators on the plant regeneration from leaf segments of Lilium callosum, L. concolor var. partheneion, and L. formosanum. Leaf segments were sectioned about 5 mm long and cultured on the basal medium (MS medium with $3\%$ sucrose and $0.8\%$ agar) under dark condition, The most effective plant regulators on harvesting more shoots from leaf culture of L. callosum were $0.2\;mg{\cdot}L^{-1}\;BA$ and $0.5\;mg{\cdot}L^{-1}\;NAA$. Culturing in the basal medium with $0.2\;mg{\cdot}L^{-1}\;BA$ and $2.0\;mg{\cdot}L^{-1}\;NAA$ was effective for leaf culture of L. concolor var. partheneion. The treatment of $1.0\;mg{\cdot}L^{-1}\;BA$ and $1.0\;mg{\cdot}L^{-1}\;NAA$ was the most effective condition for shoot harvest at the leaf culture of L. formosanum.

The Effect of Treatment of Woody Charred Materials on the Growth and Components of Tomato and Chinese Cabbage (목질탄화물의 처리가 토마토와 배추의 생육 및 체내성분에 미치는 영향)

  • Jeong, Soon-Jae;Oh, Ju-Sung;Seok, Woon-Young;Cho, Mi-Yong
    • Korean Journal of Organic Agriculture
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    • v.16 no.4
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    • pp.455-469
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    • 2008
  • A research was conducted to investigate the effect of treatment of woody charred materials such as wood vinegar, charcoal, and mixture of wood vinegar and charcoal on the growth and components of tomato and chinese cabbage. The effect of treatment of woody charred materials on the growth and components of tomato the research findings are as follows. The total number of soil microorganisms increased in the groups treated with woody charred materials compared with the control. The fruit number, fruit length, fruit diameter, fruit weight, hardness and sugar contents of tomato did not show significant difference in all plots treated with woody charred materials, but the plot treated with wood vinegar + charcoal showed relatively higher values in general. Vitamin C contents were shown higher in the plots treated with woody charred materials than the control, and among the treatment plots. The effect of treatment of woody charred materials on the growth and components of chinese cabbage the research findings are as follows. The soil chemical components did not change before and after the treatment of woody charred materials. The total number of soil microorganisms increased in the treatment plots compared with the control, The leaf length, leaf width, inner leaf number, plant weight, and head weight of Chinese cabbage treated with woody charred materials were in general larger than the control. Among the treatment plots, the plot treated with wood vinegar + charcoal showed slightly higher values. The contents of minerals in chinese cabbage such as Ca, Fe, K, Mg and Mn were higher in the treatment plots than the control.

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Micropropagation Using Shoot Tip Culture of Pyrus ussuriensis Maximowicz (정아배양에 의한 산돌배나무의 기내번식)

  • Moon, Heung-Kyu;Lee, Sung-Jae
    • Journal of Korean Society of Forest Science
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    • v.97 no.4
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    • pp.452-457
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    • 2008
  • In order to develop an efficient micropropagation technique effect of plant growth regulators (PGRs) affecting on shoot proliferation from shoot apex in Pyrus ussuriensis was tested. Generally, there was no conspicuous effect on shoot induction by the treatment of PGRs and one or two shoots/explant were induced when cultured on MS medium supplemented with BA and/or BA plus NAA. Both apical shoot necrosis and hyperhydric shoots were observed frequently in multiplied shoots, and callus was formed at the basal part of shoots. About 20% spontaneous rooting was achieved in growing shoots, however the proliferated shoots exhibited poor rooting rate in gelrite supported media. When we tried to ex vitro rooting of the shoot cutting, the shoot cuttings rooted up to 50% with 100 mg/L IBA application. The rooted plantlets grew normally after acclimatization in the greenhouse.

Effect of Cytokinins on in Vitro Growth of Grapes (Vitis spp.) (포도의 기내생장에 미치는 시토키닌의 영향)

  • Kim, Seung-Heui;Kim, Seon-Kyu
    • Journal of Plant Biotechnology
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    • v.29 no.2
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    • pp.123-127
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    • 2002
  • Effect of cytokinins (BA, TDZ, zeatin, 2iP, and kinetin) applied either singly or in combination on in vitro growth of two grape cultivars ('Cabernet Sauvignon' and 'Campbell Early') was investigated as a serial work for mass production of grapevine nursery stocks. In single treatment, shoot growth of two cultivars was most favorable in control. Shoot proliferation was satisfactory with 10 $\mu$M BA regardless of cultivars and cytokinin combinations, followed by TDZ. Other treatments resulted in very poor or no branching. Total explants ready for subculture produced by 10 $\mu$M BA outnumbered those by other treatments. TDZ was also effective. TDZ significantly increased the fresh weight and callus formation while shoot growth was unsatisfactory. Shoot growth response of two cultivars in combined treatments was also most favorable in control as was in single treatments. When TDZ was combined with zeatin, 2iP, and kinetin which failed to induce branching, proliferous branching was induced though the shoot number was behind that of single treatments of BA and TDZ. TDZ was very effective for total number of explants and fresh weight, showing 10-fold increase.