농업생명과학연구 (Journal of agriculture & life science)

  • 제45권3호
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  • Pages.53-58
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  • 2011
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  • 1598-5504(pISSN)
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  • 2383-8272(eISSN)
경상대학교 농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)
권호 표지

좀목형 (Vitex negundo var. insica) 신초의 기내증식에 미치는 cytokinin, GA 및 IBA의 영향

Effects of cytokinins, GA, and IBA on in vitro propagation of Vitex negundo var. insica

  • 한무석 (국립산림과학원 산림생명공학과) ;
  • 문흥규 (국립산림과학원 산림생명공학과) ;
  • 박소영 (국립산림과학원 산림생명공학과) ;
  • 김용욱 (국립산림과학원 산림생명공학과) ;
  • 손석규 (국립산림과학원 자원육성연구과)
  • Han, Mu-Seok (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Moon, Heung-Kyu (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Park, So-Young (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Kim, Yong-Wook (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Son, Suk-Gu (Division of tree improvement, Korea Forest Research Institute)
  • 투고 : 2011.04.25
  • 심사 : 2011.06.23
  • 발행 : 2011.06.30
⟨ 이전 논문 다음 논문 ⟩

초록

향료 및 약용 수종으로 가치 있는 좀목형 (Vitex negundo var. insica)의 기내증식 기술을 개발하고자 3년생 나무의 당년생 신초지로부터 채취한 신초의 기내 증식 및 발근에 미치는 생장조절제의 효과를 구명하였다. 다경줄기 유도는 0.5-2.0 mg/L BA가 첨가된 WPM 배지에서 효과적이었고, 가장 많은 줄기 수 (7.9개/절편)는 1.0 mg/L BA 농도에서 얻었다. 줄기 생장은 WPM 기본배지에서 1-2개의 우세 줄기로 자라는 특징이 있었고 길이는 3.4 cm 정도이었다. BA 2.0 + GA 0.5 mg/L 혼용처리는 증식과 더불어 생장을 촉진하는 효과를 보였다. 줄기 증식 시 처리된 생장조절제는 차후의 발근에 영향을 미치는 것으로 나타났다. 전반적으로 BA 처리로 유도된 줄기는 차후 발근도 잘되었으나 고농도 BA 처리 (4.0 mg/L)는 발근을 억제하였다. 저농도의 TDZ 처리도 BA 처리와 유사한 경향을 보였으나 0.5 mg/L 농도로 유도된 줄기는 발근이 현저히 억제되었고 그 이상의 농도에서 유도된 줄기는 전혀 발근되지 못했다. 증식 시 BA에 IBA를 혼용처리하면 증식은 물론 차후의 발근에 가장 좋은 것으로 나타났다. 이상의 결과는 약용가치가 뛰어난 좀목형의 기내배양을 통한 효율적인 증식 가능성을 보여주었다.

To develop an efficient micropropagation technique for Vitex negundo var. insica, which is known as aromatic and medicinal tree, the effects of various plant growth regulators (PGRs) on in vitro shoot proliferation and rooting were evaluated using the newly-developed shoots of a 3-year-old tree. Multiple shoot induction was achieved effectively on WPM (woody plant medium) supplemented with 0.5-2.0 mg/L BA, and the highest shoot number (7.9/explant) was obtained at the concentration of 1.0 mg/L BA. Typically 1 or 2 superior shoots (about 3.4 cm) were induced on hormone-free WPM. Combined treatment of BA 2.0 + GA 0.5 mg/L appeared to effective on shoot proliferation and rooting. Plant growth regulators added in shoot proliferation medium had strong impact on subsequent rooting as well. Overall, shoots induced by BA treatment resulted in high rooting rates while the effect was reduced gradually by ascending BA levels. TDZ of low concentration also revealed a similar tendency as BA, but the rooting ability was strongly inhibited at the concentration of 0.5 mg/L, and rooting was never observed at the concentrations higher than 0.5 mg/L. Combined treatment of BA and IBA had positive influence in both shoot proliferation and rooting. These results suggest that Vitex negundo var. insica could be effectively micropropagated via axillary bud cultures.

키워드

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