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Micropropagation Using Shoot Tip Culture of Pyrus ussuriensis Maximowicz  

Moon, Heung-Kyu (Div. Biotechnology, Korea Forest Research Institute)
Lee, Sung-Jae (Forest Research Institute of Kangwon Provience)
Publication Information
Journal of Korean Society of Forest Science / v.97, no.4, 2008 , pp. 452-457 More about this Journal
Abstract
In order to develop an efficient micropropagation technique effect of plant growth regulators (PGRs) affecting on shoot proliferation from shoot apex in Pyrus ussuriensis was tested. Generally, there was no conspicuous effect on shoot induction by the treatment of PGRs and one or two shoots/explant were induced when cultured on MS medium supplemented with BA and/or BA plus NAA. Both apical shoot necrosis and hyperhydric shoots were observed frequently in multiplied shoots, and callus was formed at the basal part of shoots. About 20% spontaneous rooting was achieved in growing shoots, however the proliferated shoots exhibited poor rooting rate in gelrite supported media. When we tried to ex vitro rooting of the shoot cutting, the shoot cuttings rooted up to 50% with 100 mg/L IBA application. The rooted plantlets grew normally after acclimatization in the greenhouse.
Keywords
Chinese pear; shoot multiplication; rooting; acclimatization;
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1 권영진, 윤양, 이석구, 현영일, 이정주, 이문호. 1990. 호도나무 아배양에 의해 증식된 줄기의 in vivo 발근. 임육연보 26: 63-68.
2 Donnini, S., Cinelli, F., Sensale, L., Muleo, R., Zocchi, G. and Ranieri, A. 2008. Pear plantlets cultured 'in vitro' under lime-induced chlorosis display a better adaptive strategy than quince plantlets. Plant Cell Tiss. Org. Cult. 93: 191-200.   DOI
3 Lucyszyn, N., Quoirin, M., Ribas, L.L..F., Koehler, H.S., and Sierakowski, M.R. 2006. Micropropagation of 'Durondeau' pear in modified-gelled medium. In Vitro Cell. Dev. Biol-Plant. 42: 287-290.   DOI   ScienceOn
4 Palombi, M.A., Lombardo, B. and Caboni, E. 2007. In vitro regeneration of wild pear (Pyrus pyraster B.) clones tolerant to Fe-chlorosis and somaclonal variation analysis by RAPD markers. Plant Cell Rep. 26: 489-496.   DOI
5 Raharjo, S.H.T. and Litz, R.E. 2005. Micrografting and ex vitro grafting for somatic embryo rescue and plant recovery in avocado (Persea americana). Plant Cell Tiss. Org. Cult. 82: 1-9.   DOI
6 Sha, I., McCown, B.H., and Peterson, L.A. 1985. Occurrence and cause of shoot-tip necrosis in shoot cultures. J. Amer. Soc. Hort. Sci. 110: 631-634.
7 Yancheva, S.D., Shlizerman, L.A., Golubowicz, S., Yabloviz, Z., Perl, A., Hanania, U. and Flaishman, M.A. 2006. The use of green fluorescent protein (GFP) improves Agrobacterium-mediated transformation of 'Spadona' pear (Pyrus communis L.). Plant Cell Rep. 25: 183-189.   DOI
8 Pierik, R.L.M. 1991. In vitro culture of higher plants. Martinus Nijhoff Pub., pp 344
9 Lane, W.D., Iketani, H. and Hayashi, T. 1998. Shoot regeneration from cultured leaves of Japanese pear (Pyrus pyrifolia). Plant Cell Tiss. Org. Cult. 54: 9-14.   DOI
10 Nee, C.C. Tasi, C.H. and Anstine, D.D. 2002. Asian pear germplasm-future trends and current research in the industry. Acta Hort. 587: 61-69.
11 Suh, J.H., Jung, J.D., and Kwon, O.C. 2001. Virus free stock production by in vitro stem cutting of shoot tip cultures of grapes. Korean J. Plant Tiss. Cult. 28: 81-85.
12 Shibi. R.A., Ajlouni, M.M., Jaradat, A., Aljanabi, S., and Shatnawi, M. 1997. Micropropagation in wild pear (pyrus syrica). Scientia Horticulture. 68: 237-242.   DOI   ScienceOn
13 Matsuda, N., Gao, M., Isuzugawa, K., Takashina, T. and Nishimura, K. 2005. Development of an Agrobacteriummediated transformation method for pear (Pyrus communis L.) with leaf-section and axillary shoot-meristem explants. Plant Cell Rep. 24: 45-51.   DOI
14 김명조, 임요섭, 송원섭, 김이훈, 유창연. 1999. 산돌배나무(Pyrus ussuriensis Maximowicz) 열매의 항산화 물질분리 및 구조. 한작지. 7(4): 303-307.
15 Jackson, J.E. 2003. Biology of apples and pears. Cambridge: Cambridge University Press, 22.
16 Poudyal, B.K., Zhang, Y. and Du, G. 2008. Adventitious shoot regeneration from the leaves of some pear varieties (Pyrus spp.) grown in vitro. Front. Agric. China. 2(1): 82-92.   DOI
17 Baek, K.Y., Yoo, K.J. and Park, S.I. 1998. In vitro propagation of Rehmannia glutinosa. Korean J. Plant Tiss. Cult. 25: 63-68.   과학기술학회마을
18 Chevreau, E., Mourgues, F., Neveu, M., Chevalier, M. 1997. Effect of gelling agents and antibiotics on adventitious bud regeneration from in vitro leaves of pear. In Vitro Cell. Dev. Biol-Plant. 33: 173-179.   DOI
19 George, E.F. 1996. Plant propagation by tissue culture. Part 2. In Practice, pp. 575-732. Exegetics Limited Press.
20 Predieri, S. and Zimmerman, R.H. 2001. Pear mutagenesis: In vitro treatment with gamma-rays and field selection for productivity and fruit traits. Euphytica 117: 217-227.   DOI
21 Caboni, E., Tonelli, M.G., Lauri, P., D'Angeli, S., and Damiano, C. 1999. In vitro shoot regeneration from leaves of wild pear. Plant Cell Tiss. Org. Cult. 59: 1-7.   DOI
22 Kadota, M. and Niimi, Y. 2004. Production of triploid plants of Japanese pear (Pyrus pyrifolia Nakai). Euphytica 138: 141-147.   DOI   ScienceOn
23 Sul, I.L. and Shin, D.I. 1996. Effect of antibiotic washes on reduction in stem explants of greenhouse-grown Populus deltodies. Korean J. Plant Tiss. Cult. 23: 355-361.
24 Jun, J.H., Yae, B.W., Yang, M.H., and Park, J.B. 1997. Influence of growth regulators on adventitious shoot regeneration from tissues of Malus domestica cv. 'Gala' in vitro. Korean J. Plant Tiss. Cult. 24: 125-128.
25 김정호. 2001. 최신배 재배, 오성출판사, pp. 134-135.
26 Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15: 437-497.   DOI
27 Durkovic, J. 2006. Rapid micropropagation of mature wild cherry. Biol. Plant. 50(4): 733-736.   DOI
28 이창복. 2003. 원색 대한식물도감(상). 향문사 pp. 914.
29 Shen, X.S. and Mullins, M.G. 1984. Propagation in vitro of pear, Pyrus communis L. cultivars 'William's BonChretien', 'Packam's Triumph' and 'Beurre Bosc'. Sci. Hort. 23: 51-57.   DOI   ScienceOn
30 Silos-Espino, H., Valdez-Ortiz, A., Rascon-Cruz, Q., Rodriguez-Salazar, E. and Paredes-Lopez, O. 2006. Genetic transformation of prickly-pear cactus (Opuntia ficusindica) by Agrobacterium tumefaciens. Plant Cell Tiss. Org. Cult. 86: 397-403.   DOI
31 Kadota, M., Niimi, Y. 2003. Effects of cytokinin types and their concentrations on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots. Plant Cell Tiss. Org. Cult. 72: 261-265.   DOI   ScienceOn