• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.301-304
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• 2002

Perfusion culture strategies for high density culture of plant cell suspensions to enhance the productivity of extracellular polysaccharides were investigated. Angelica gigas Nakai cell suspensions were used to produce the extracellular polysaccharide and perfusion parameters were optimized to maximize the production. When the medium exchange was started at the fifth day after inoculation, the maximum cell concentration (23.8 g dry cell weight per liter) was achieved.

• KSBB Journal
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• v.7 no.3
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• pp.172-178
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• 1992

A simple and effective method has been developed for the immobilization of lipase producing Rhizopus chinensis on polyurethane foam. In this method, the fungal cells with 1, 3 specific lipase in there inside are immobilized within the foam matrix. Four types of commercially available polyurethane foam were tested. The ultimate purpose of the process is to produce low-cost biocatalysts for lipase-catalyzed reactions, which are being increasingly used for industrial applications. Effects of several parameters were studied on the cell loading and the hydrolytic activity of intracellular lipase after acetone drying. These parameters were the type, size, and amount of polyurethane foam. In all the cases, the intracellular lipase activity obtained with the foam was approximately twice greater than that obtained in the absence of the foam.

• Korean Journal of Plant Tissue Culture
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• v.22 no.5
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• pp.283-290
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• 1995

This experiment was canted out to obtain embryogenic callus and to understand developmental mechanism of somatic embryogenesis in Oenanthe javanica ($B_{L.}$) DC. experiments included the examination of explant source and media for embryogenic callus production and the observation of developmental pattern of embryogenic cells and non-embryogenic cells. Embryogenic calli were formed on zygotic pro-embryos together with their endosperms when they were cultured on Ms media containing 1.0mg/L 2,4-D. Embryogenic calli were also formed on the intact surface in vitro grown stem or petiole segmentsafrer 6-8 weeks of culture, whereas non-embryogenic calli were formed on cut surfaces of the stem and petiole after 2 weeks of culture. Non-embryogenic calli were rhizogenic in suspension and solid media culture.

• Korean Journal of Plant Tissue Culture
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• v.21 no.2
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• pp.77-84
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• 1994

To assess the feasibility of anthocyanin production in cell cultures of Euphorbia splendens Bojer the role of sucrose in pigment production was investigated and pilot scale cultures were attempted to establish mass production system. And also, several instrumental analyses were conducted to identify the pigment extracted from cultured rolls. Anthocyanin production was promoted prominently with concenetrations of sucrose ranging from 3% to 9% while cell growth was maximized at 3% of sucrose . This suggested that high osmolarity of sucrose enhance pigment production. When cells were cultured in two types of bioreactor better cell growth was achieved with draft-type air lift bioreactor than impeller type bioreactor and the pigment productivity was reached to 2.2 mg/L/day. The major pigment extracted from cultured cells was characterized as cyanidin-3-glucoside.

• Applied Biological Chemistry
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• v.48 no.4
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• pp.425-430
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• 2005

[${\beta}-sitosterol$] is a plant sterol that reduces cholesterol levels and inhibits the growth of human prostate and colon cancer cells. Optimal conditions for ${\beta}-sitosterol$ production were examined from cell suspension cultures of Chrysanthemum coronarium L. The callus induction was optimal in MS medium containing 1 mg/l NAA and 1 mg/l BAP. Cell suspension culture was also established from the callus. Optimal ${\beta}-sitosterol$ production was obtained when the cells were cultured at an initial density of 2 mg DCW/l in MS medium containing 1 X sucrose (30 mg/l), 1 X nitrogen (1900 mg/l $KNO_3$, 1650 mg/l $NH_4NO_3$), and 1 X phosphate source (170 mg/l). In cell suspension cultures of C. coronarium L. using shake flasks, the peak content of ${\beta}-sitosterol$ was $150{\mu}g/g$ DCW. In cell suspension cultures of C. coronarium L. using an air-lift bioreactor, the maximum ${\beta}-sitosterol$ content of $143.8{\mu}g/g$ DCW was obtained at an air-flow rate of 100 cc/min.

• 한국약용작물학회:학술대회논문집
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• 2007.05a
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• pp.199-200
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• 2007

• Proceedings of the Korean Society of Crop Science Conference
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• 1991.05a
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• pp.108-109
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• 1991

• Proceedings of the Korean Society of Crop Science Conference
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• 2001.09a
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• pp.148-149
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• 2001

• Proceedings of the Korean Society of Crop Science Conference
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• 1995.05a
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• pp.12-13
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• 1995

• Korean Journal of Plant Tissue Culture
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• v.24 no.6
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• pp.361-367
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• 1997

This study was carried out to select cadmium resistant cell lines from leaf-derived calli of diploid and haploid of Nicotiana tabacum cv. BY4, for understanding adaptation mechanism of plants in cadmium contaminated environment. suspended cell clumps were plated onto selection medium containing 0 to 2,000 $\mu$M cadmium. Cadmium resistant colonies were formed on the selection medium after 3 or 4 weeks of culture. The minimum inhibition concentration (MIC) of cadmium on colony formation were 300 $\mu$M in diploid and 200 $\mu$M in haploid plants, respectively. In order to test the resistance to cadmium, selected cell line on MIC were transferred to medium containing high concentration of cadmium. The selected cell lines, especially haploid cell line, were resistant an the high concentration of cadmium. And dry weight, ash weight, and cadmium contents of cell were increased. These results indicated that the selected cell lines showed higher resistance of cadmium than control cells, and haploid plant is more resistant than diploid plant on medium with cadmium.