• Horticultural Science & Technology
• /
• v.29 no.3
• /
• pp.247-254
• /
• 2011

The agronomic characteristics, nutritional contents, and insect response of the potato clones transformed with a glufosinate ammonium resistance gene were evaluated. Among the 4 transgenic potato clones, the Bar 3 clone was selected as a promising one for commercialization. The Bar 3 clone showed similar tuber yield capacity but higher herbicide resistance as compared with the non-transgenic potato cv. Dejima. The herbicide resistance of the Bar 3 clone was more than 5 times higher when tested with the herbicide concentration recommended by the producer. The major agronomic characteristics of the Bar 3 clone were not different from those of the non-transgenic Dejima. The annual variation in yields and agronomic characteristics showed similar tendency for 2 years from the third to fourth generation after transformation. The tubers of the Bar 3 clone also showed low occurrence in common scab and physiological disorders such as cracking and secondary growth. But the reasons for such results are yet to be studied. Also, it was considered that the Bar 3 clone have a potential of reducing not only common scab occurrence but also soil erosion during potato cultivation in field. The nutritional contents (mineral compound, vitamin C and amino acid) and response to Spodoptera exigua of the transgenic potato clones were not significantly different.

• Korean journal of applied entomology
• /
• v.24 no.4 s.65
• /
• pp.195-201
• /
• 1986

This study was conducted to investigate the life history of Compsidia populnea which is major species of the stem-borers on Populus alba{\times}glandulosa$. Peak emergence of the adult of Compsidia populnea L. was around 10th${\sim}$l2nd of May in Chuncheon vicinities, having one generation a year in Korea. There was a preovipositional period of 10.7 days and a ovipositional period of 14.3.days. Mean adult longevity was 11 days for male and 13.8 days for female. Individual eggs were long oval with somewhat narrowed tip at one side and soft, 2.6mm in length, 0.8mm in diameter, and were laid singly under the U-shaped scars which were made by female before oviposition. Total number of scars per female averaged 56.6 ($8{\sim}135$) and eggs were found in 67.9 percent of the total scars. Egg period was $8{\sim}11$ days at $25^{\circ}C$ constant temperature and $7{\sim}14$ days in the field condition. Early young larvae stayed for $2{\sim}3$ weeks under the scars and then boring into the xylem, forming galls at this time. Size of the gall was 1.8cm($1.3{\sim}2.5$) in length, 1.6cm($1.0{\sim}2.2$) in diameter and the length of mine was everage 3.1cm. Fully grown larvae were developed around the early October and overwintered in the mines of galls. Pupation was done around the early of April. Pupal developmental period averaged 11 days($9{\sim}13$ days) at $25^{\circ}C$ constant temperature condition.

• Korean journal of applied entomology
• /
• v.52 no.4
• /
• pp.431-436
• /
• 2013

Ricania sp. lay eggs into the new twigs of blueberry and suck nutrients causing growth retardation and a sooty mold symptom. This study was conducted to investigate the emergence success and developmental period of overwintered eggs of Ricania sp. under the seven constant temperatures from 10 to $34^{\circ}C$ by $4^{\circ}C$ intervals (14L:10D). We also monitored the emergence time of the Ricania sp. nymph at blueberry by 3 ~ 4 day interval using a modified leaf clip cage. Development times from the overwintered egg to $1^{st}$ nymph were 107.1, 54.5, 33.9, 25.3, 25.1 and 16.7 days and the emergence successes were 23.1, 30.8, 13.8, 21.7, 11.9, and 0.6% at 14, 18, 22, 26, 30 and $34^{\circ}C$, respectively except at $10^{\circ}C$. The developmental periods were decreased with increasing temperatures. First emergence date of the nymphs in the field was between 19 and 22 May, and the average emergence success was 19.6%. The present study might be helpful to establish the management strategy of Ricania sp. based on the biological characteristic.

• Korean journal of applied entomology
• /
• v.49 no.1
• /
• pp.37-42
• /
• 2010

As an environment-friendly phytosanitary measure, CATTS (controlled atmosphere temperature treatment system) has been developed to kill several quarantine insect pests infesting subtropical agricultural commodities. This study tested any possibility to apply CATTS to apples to effectively eliminate the peach fruit moth, Carposina sasakii, which has been regarded as a quarantine insect from the imported countries. When the larvae of C. sasakii were directly exposed to $46^{\circ}C$ (an installed lethal temperature of CATTS), they showed a median lethal time at 14.66 min. Addition of high carbon dioxide to the temperature treatment enhanced the thermal limit susceptibility of C. sasakii to $46^{\circ}C$. CATTS device was constructed to automatically control $CO_2$ concentration and temperature with real-time monitoring both in the chamber and in the fruit. The larvae internally infesting apples were tested using the CATTS device and showed 100% lethality after 60 min exposure to a treatment of $46^{\circ}C$ under 15% $CO_2$ in the chamber. Relatively long exposure may be due to the deviation between the ramping temperature ($0.35^{\circ}C$/min) of the chamber and the ramping temperature (0.12-$0.23^{\circ}C$/min) inside apple fruit, where the tested larvae were located. This study suggests a possibility that CATTS can be applied as a quarantine measure to kill the larvae of C. sasakii locating inside the apples.

• Journal of Life Science
• /
• v.28 no.3
• /
• pp.369-375
• /
• 2018

Mycoplasma genitalium has 367 conserved genes and the smallest genome among mono-culturable prokaryotes. Conservative metabolic pathways were examined among M. genitalium and 14 prokaryotes, one hyperthermophilic exosymbiotic archaeon Nanoarchaeum equitans and 13 intracellular eubacteria of plants or insects, with fewer conserved genes than M. genitalium. They have 11 to 71 metabolic pathways, however complete metabolic pathways ranged from 1 to 24. Totally, metabolic pathway hole is very high due to the lack of 45.8% of the enzymes required for the whole metabolic pathways and it could be suggested that the shared metabolic pathway with the host's enzyme would work or the essential substances are host dependent. The number of genes necessary for mass transfer through the cell membrane is also very low, and it may be considered that the simple diffusion or the protein of the host will function in the cell membrane of these prokaryotes. Although the tRNA charging pathway was distributed in all 15 prokaryotes, each has 5-20 tRNA charging genes. This study would give clues to the understanding of the metabolic pathways of intracellular parasitic bacteria of plant and endosymbiotic bacteria of insects, and could provide basic data for prevention of crop damage, development of insect pests and human medicines.

• Korean Journal of Agricultural Science
• /
• v.30 no.1
• /
• pp.11-19
• /
• 2003

The life-cycle of the aphid-parasite, Aphidius gifuensis, took 11.9 days at $25^{\circ}C$ and 12.1 days at $30^{\circ}C$. The female appearance rate of A. gifuensis was the best at $20{\sim}25^{\circ}C$ compared to the other temperatures. Optimum rearing temperature of Aphidius gifuensis was $20{\sim}25^{\circ}C$ Optimum temperatures for A. gifuensis emergence were $20^{\circ}C$ and $25^{\circ}C$ with 99.1% and 98.2%, respectively. However the emergence rate of Aphidius gifuensis was 58.8% at $10^{\circ}C$. There was a positive correlation between A. gifuensis adult activity and temperature, but there was a negative correlation between developmental period of Myzus persicae nymph and temperature. Among the pests occurring in pepper greenhouse, dominant species was M. persicae and its density was 6~1,024 per plant. The damaged fruit rate by Helicoverpa assulta was 3.3~53.3%. The number of aphid and mummy in the A. gifuensis released plot were 173.5 and 10 in June, 1.8 and 17 in July, 2000, respectively. The numbers of Aphidius gifuensis were 7.5 and 0.4 in May, 27.1 and 2.1 in June, 2001, respectively. The suppressive effects on M. persicae in A. gifuensis released plot was better than the control plot, but there was no significant difference compared to the pesticide-sprayed plot. The first leaf length and the stem width in the A. gifuensis released plot, the control plot, and the pesticide-sprayed plot were not significantly different, but the A. gifuensis released plot showed the highest yield among the plots.

• Journal of Korean Society of Forest Science
• /
• v.105 no.1
• /
• pp.42-47
• /
• 2016

To examine the natural regeneration in the natural mixed-broadleaved forest, flowering, fruiting, seed-fall, and seed viabilities of Acer pseudosieboldianum (Ap) were investigated in Mt. Jungwang, Gangwon-do, from 2009 to 2015. The flower of Ap consisted many male and bisexual flowers on the corymb. Flowering dates are differed between sex morph in the same inflorescence. Stamens are stop growing and disappeared after pollinated pistil begin to grow in bisexual flowers, and male flowers have vestial pistil. The flowers of Ap might be pollinated by Apis mellifera, Andrenidae spp. and Syrphinae spp. Ap had some mechanism to prevent from self pollination with heterodichogamy. Mean annual seedfall of Ap was 70,780 ea/ha (ranged 310~234,840 ea/ha). Annual seedfall of Ap varied severely, and the maximum was about 760 times the minimum. Annual seed production of Ap might be to a normal distribution. Rates of damaged or decayed seeds are highest 59.3%, and those of sound seeds are 23.9%, Those of undeveloped and empty seeds are 9.2% and 7.6%, respectively. The most important factors influencing sound seed production might be the density and activities of insect pollinators and sucking pest in the flowerwing period, middle-late May. Successful regeneration of Ap might be in masting year and on the gap sites with proper conditions to germinate and grow. To understand the natural regeneration of deciduous hardwoods, further study on the characteristics of flowering and fruiting, pre- and post-dispersal seed predation, and annual variation on these factor should be needed.

• Journal of Korean Society of Forest Science
• /
• v.103 no.2
• /
• pp.153-158
• /
• 2014

To examine the natural regeneration in the natural mixed-broadlived forest, flowering, fruiting, seed-fall, and seed viabilities of Acer ukurunduense Trautv. & C.A. Mey. (AU) were investigated in Mt. Jungwang, Gangwon-do, from 2009 to 2013. The flower of AU consisited many male and bisexual flowers on the raceme (unusual panicle) and the arrangement of two sex morphs are differed for each inflorescence. Flowering dates are differed between sex morph in the same inflorescence. Stamens are stop growing and disappeared after pollinated pistil begin to grow in bisexual flowers, and male flowers have vestial pistil. The flowers of AU might be pollinated by Apis mellifera, Thyris fenestrella seoulensis, Cerambycidae sp., Andrenidae sp. and Ctenophora sp., and had some mechanism to prevent feom self pollination. The number of flower buds per inflorescence is 189 on June 8, and that of young samaras per inflorescence is 41.2 on June 21. At last ripened samaras per inflorescence is reduced 33.4 on September 5. Mean annual seedfall of AU was 6,720 ea/ha (ranged 670~17,930). Rates of sound seeds are highest 43.2%, and those of damaged or decayed seeds are 41.8%,. Those of undeveloped and empty seeds are 10.2% and 4.8%, respectively. Successful regeneration of AU might be in masting year and on the gap sites with proper conditions to germinate and grow. To understand the natural regeneration of the species, Genus Acer, further study on the fruiting habit, pre- and post-dispersal seed viability, and annual variation on these factor should be needed.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
• /
• v.19 no.2
• /
• pp.81-85
• /
• 2013

The stored-product insects have been a serious problem during the entire process of distribution on the food industry. Especially, the Indian meal moth (Plodia interpunctella) is one of stored-product insects which causes harm through penetrating into the food packaging. The objective of this study was to develop the anti-insect packaging material with fennel (Foeniculum vulgare) oil (FO). The FO has been selected for insecticidal substance against P. interpunctella, which was tested by fumigant toxicity assay. An anti-insect sachet was prepared by FO and filterpaper placed in a small paper bag. Repellent test was performed to evaluate the repellent activity of anti-insect sachet. In addition, the controlled release of FO from the anti-insect sachet was determined at $28^{\circ}C$ by gas chromatography (GC). It was demonstrated that FO was an effective substance against P. interpunctella. The mortality of FO was 56% at 800 mg/0.5 L in 120 h. In repellent test, the FO sachet showed effective repellency against P. interpunctella. The developed anti-insect sachet could be a promising source for insect repellent materials in food packaging.

• The Korean Journal of Pesticide Science
• /
• v.20 no.1
• /
• pp.56-65
• /
• 2016

Pine wood nematode, Bursaphlenchus xylophilus is a fatal damaging pest, infecting pinewood trees and is one of the serious forest pest in Korea. Trunk injection is one of the most effective method to protect the live pine trees from nematode infection. The aim of this research was to reveal the efficacy of the nematicidal materials from 46 Kyrgyzstan, 88 Vietnam plant materials and 21 active materials (${\alpha}$-terpinene, ${\alpha}$-pinene, ${\beta}$-thujaplicin, cinnamaldehyde, eugenol, emodin, geraniol, limonone, methyl palmitate, matrine, myrcene, methyl gallate, nicotine, quassin, resveratrol, rotenone, thymol, thujaplicin, terthiophene, tuberstemonine, ${\gamma}$-terpinene) isolated from plants for using as trunk injection agents. Eclipta prostrata extract was highly effective against pine wood nematode in plant extracts and terthiophene has the best nematicidal activity from active materials isolated from plants. Foliar spray of terthiophene on Japanese black pine (Pinus thunbergii) tree reduced proliferation of inoculated pine wood nematode at 22 days after treatment, however no efficacy was found over 22 days. Eclipta prostrata extract and terthiophene can be used as nematicide for further nematicidal efficacy test against any other plant parasitic nematodes and trunk injection efficacy against pine wood nematode.