• Journal of Physiology & Pathology in Korean Medicine
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• v.36 no.4
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• pp.125-129
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• 2022

The purpose of study is to evaluate in vitro anti-oxidant and anti-inflammatory effects of Moringa Folium and Eucommiae Cortex 2:1 (g/g) mixtures (MEMix). HaCaT and human normal dermal fibroblast were treated with 0.01-1 mg/mL of MEMix to monitor cytotoxicity. Radical scavenging activities of MEMix were examined by DPPH assay. To explore anti-inflammatory effect, Raw 264.7 cells were pretreated with MEMix for 1h and subsequently exposed to LPS for 18h. NO release and cytotoxicity of Raw 264.7 cells were measured by adding Griess and MTT reagents, respectively. TNF-α, IL-1β, IL-6, and PGE2 productions were examined by ELISA. Immunoblot analysis was conducted to examine COX-2 expression in MEMix pretreated Raw 264.7 cells. Up to 1 mg/mL concentration, treatment of MEMix for 24 h did not affect normal dermal fibroblast viability and significantly reduced cell viability of HaCaT cells with no concentration dependency. MEMix increased DPPH radical scavenging activity with concentration dependency. Radical scavenging activities by 1 mg/mL of MEMix was comparable with 30 µM of trolox. Pretreatment of MEMix did not change the reduction of Raw 264.7 cell viability. Exposure of LPS in Raw 264.7 cells significantly increased NO, TNF-α, IL-1β, IL-6, and PGE2 productions, and MEMix pretreatment attenuated these productions by LPS concentration dependently. However, pretreatment with MEMix did not change COX-2 expression by LPS in Raw 264.7 cells. MEMix showed in vitro anti-oxidant and anti-inflammatory activities. MEMix would be useful candidate agent against inflammation.

• Journal of dental hygiene science
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• v.5 no.4
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• pp.251-258
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• 2005

The objective of this research was to analyze curriculum of dental hygiene education program for B.S degree in US and compare with Korea and Japan. The curriculum was classified six domain based on job classification and National Board Examination in Korea. Oral biology content included oral anatomy,dental anatomy,oral histology,oral pathology. Oral physiology was excluded. Clinical dentistry content included only oral radiology, periodontics, dental material,pain control. Most program integrated clinical dental hygiene courses. Most program provided dental practice management content and dental hygiene research courses. Diverse program such as A.S degree,B.S degree,degree completion,distance education programs enabled students to develop their career effectively.

• Journal of Physiology & Pathology in Korean Medicine
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• v.29 no.5
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• pp.416-421
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• 2015

This study was performed to comparative investigate the distribution of primary sensory and motor neurons associated with Daereung(PC7) acupoints by using neural tracing technique. A total 16 SD rats were used in the present study. After anesthesia, the rats received microinjection of 6 ㎕ of cholera toxin B subunit(CTB) into the corresponding sites of the acupoints Daereung(PC7), in the human body for observing the distribution of the related primary sensory neurons in dorsal root ganglia(DRGs) and motor neurons in the spinal cord(C3∼T4) and sympathetic ganglia. Three days after the microinjection, the rats were anesthetized and transcardially perfused saline and 4% paraformaldehyde, followed by routine section of the DRGs, sympathetic chain ganglia(SCGs) and spinal cord. Labeled neurons and nerve fibers were detected by immunohistochemical method and observed by light microscope equipped with a digital camera. The labeled neurons were recorded and counted. From this research, the distribution of primary sensory and motor neurons associated with Daereung(PC7) acupoints were concluded as follows. Muscle meridian related Daereung(PC7) controlled by spinal segments of C5∼T1, C6∼T4, respectively.

• The Korean Journal of Emergency Medical Services
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• v.8 no.1
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• pp.169-178
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• 2004

본 연구의 목적은 응급구조사들이 긴 척추고정판을 이용하여 환자를 들어 올리는 3가지 다른 자세와 다른 3가지 부하 조건을 이용하여, 요추 4번과 5번의 압축력, 전단응력 그리고 합력의 변화를 동역학적 모델을 제시하기 위한 분석이다. 연구방법 : 36명의 남자가 본 연구의 실험에 자발적으로 동원되었으며, 나이는 평균 21.42세이고, 신장은 평균 174.05cm이며, 체중은 평균 78.05kg이다. 이 실험에서 부하 조건은 50, 70, 90kg이고, 들어 올리는 높이는 지상에서부터 95cm 이었으며, 들어 올리는 동안의 회전고리는 110cm이었다. 운동현상학적 자료는 2-D ProReflex Motion Capture Camera을 이용하였으며, sampling rate는 60Hz로 하였다. 결과 및 논의 : 동역학적 데이터 자료를 근거로 한 본 연구의 결론은 다음과 같다. Lunge 자세기술에서 전단응력과 합력 등이 최소의 stress로 요추 4번 5번에 미치는 것으로 나타났다. 그러나 Lunge 기술에서 압축력은 약간 증가되는 것으로 나타났다. 이 연구에서 Stooped 자세기술에서는 아주 큰 전단응력과 합력 등이 요추 4번, 5번 관절에 넓게 작용하는 것으로 나타났으며, 이는 들어 올리는 동작을 할 때 상해의 원인이 된다고 사료된다. 특히 응급구조사들이 들것을 들어 올릴 때 너무 큰 전단응력이 요추 4번, 5번 관절에 작용을 하면 비정상적으로 병리학상 또는 해부학상 신체적변화가 온다고 해석할 수 있다. 그래서 응급구조사들에게 들것을 들어올리는 stooped 자세는 아주 크고 많은 합력 작용하기 때문에 권고될만한 기술이 아니라고 해석 된다. Squat 자세에서 중간 정도의 압축력, 전단응력 그리고 합력이 작용된다. 만약 응급구조사가 전단응력 그리고 합력이 요추에 미치는 영향이 가장 걱정된다면, lunge 자세가 두 가지 힘을 줄여줄 수 있다고 사료된다. 마지막으로 응급구조사가 들것을 들어올리는 데는 squat 자세 기술이 가장 좋다고 사료 된다.

• Journal of Physiology & Pathology in Korean Medicine
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• v.33 no.1
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• pp.10-16
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• 2019

In order to investigate the effects of Eucomiae Cortex extracts on the depression caused by aging, histochemistry and immunohistochemistry were performed on the hippocampus of aged rats and the following results were obtained. Experimental animals were divided into three groups as follows: 8 week old ICR male mice, Aging-elicited group (AE group) and Eucomiae Cortex treatment group (EC group) 50 week old male ICR mice were used. The control group and AE group did not take any treatment and did not restrict diets and negatives. In the EC group, 0.51g/kg of Eucomiae Cortex extract was dissolved in distilled water once a day for 6 months. The Eucomiae Cortex extract reduced pyramidal neuronal damage in C3 hippocampus and dentate gyrus, increased DJ-1, SHH positive responses in aged mouse hippocampus, and 8-OHdG positivity was reduced, ${\beta}$-endorphin positivity was reduced in aged mouse substantia nigra. Therefore, based on the above results, Eucomiae Cortex extract reduces damage of pyramidal neurons in the hippocampus caused by aging, inhibits neuronal cell death, induces proliferation and differentiation of stem cells in the hippocampus, reduces DNA damage-induced oxidative stress, so improves the reduction of hippocampus volume. It is also thought to improves depression due to aging through ${\beta}$-endorphin which enhances mood through the inhibition of pain.

• Journal of Physiology & Pathology in Korean Medicine
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• v.33 no.1
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• pp.25-30
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• 2019

This study was aimed to examine the synergetic effects of photochemopreventive external agents composed of inorganic ZnO and esculetin. Zinc oxide (ZnO) is widely used in sunblocks because of its excellent biostability and little toxicity. Esculetin derived from Citrus Peel has an anti-oxidative effect. We made the hollow-shaped ZnO microsphere (MS), soaked it with esculetin (EZnO). We used SKH-1 mice to measure the photodamaging effects of UVB. The mice were divided into five groups as follows; UVB nontreated group (N), vehicle (C), esculetin (E), ZnO MS (ZnO), esculetin + ZnO MS(EZno) group. Each group of samples was topically applied to the dorsal skins before the UVB irradiation. The changes of collagen fibers in the skin tissues were observed by H & E staining and Van Gieson staining. The expression of mast cells in skin tissue was observed by immunohistochemical staining of tryptase present in the mast cell granules. Expression of inflammatory cytokines such as TNF-${\alpha}$, IL-$1{\beta}$, and MMP-9, which plays an essential role in wrinkle formation, was measured by RT-PCR. Interestingly, the composition of collagen fibers was better in the EZnO applied group than in the E or ZnO group. Moreover, mast cell expression and the expression of TNF-${\alpha}$, IL-$1{\beta}$, and MMP-9 mRNA were markedly suppressed in the EZnO group, indicating that the synergetic effects of esculetin and ZnO were excellent.

• Journal of Physiology & Pathology in Korean Medicine
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• v.33 no.1
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• pp.56-62
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• 2019

To investigate the effect of Bombycis Corpus on male osteoporosis, we performed Dual Energy X-Ray Absorptiometry(DEXA) and histochemical methods. The animals were used ICR-based male mice of 8 weeks and 50 weeks, respectively. ICR male mice at 8 weeks were used in the control group, and ICR male mice at 50 weeks were used in aging group and Bombycis Corpus group(BC group). In the aging group, 0.5 ml of distilled water was administered once a day for 6 months. In BC group, Bombycis Corpus(0.78g/kg) was dissolved in distilled water for 6 months once a day. As a result, Bombycis Corpus decreased bone loss, increased bone density by reducing the loss of bone matrix in the femur due to aging, and increased osteoblast - induced osteopontin(OPN) and osteocalcin(OPC) positivite reaction. In addition, administration of Bombycis Corpus decreased Reaction of activation of nuclear factor kappa B ligand(RANKL) positive reaction, increased osteoprotegerin(OPG) positive reaction, and decreased matrix metalloproteinase-3(MMP-3) and 8-hydroxy-2'-deoxyguanosine(8-OHdG) positivite reaction. Taken together, Bombycis Corpus increases the activity of osteoblasts, inhibits osteoclast function, promotes osteoblast function, inhibits bone tissue degradation, and inhibits bone loss due to oxidative stress. It was observed that Bombycis Corpus reduced bone loss and increased bone density caused by aging to improve male osteoporosis. Therefore, Bombycis Corpus may be used as a preventive and therapeutic agent for male osteoporosis.

• Journal of Physiology & Pathology in Korean Medicine
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• v.36 no.1
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• pp.11-17
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• 2022

In this study, we investigated the effects of Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng extracts (FRG) on cognitive function in scopolamine-induced memory-impaired mice. We measured the effects of G, R, and FRG on the improvement of memory and cognition via behavior analysis. In addition, we measured the acetylcholinesterase (AChE) activity in the hippocampus of each group of mice. The expression of β-amyloid, Tau, and BDNF in the brain tissues were observed through immunohistochemical staining. Ginseng Radix (G) and Fermented red-ginseng (FRG) have effectively improved cognitive function in the water maze test. Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng (FRG) have improved the willingness of mice to explore the new environment, as confirmed by Y maze test. In addition, immunohistochemical staining revealed that Ginseng Radix (G) decreased the expression of β-amyloid and Tau in the hippocampus. In addition, fermented red-ginseng (FRG) increased the expression of BDNF. Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng (FRG) have decreased the concentration of acetylcholinesterase in the hippocampus as compared with the control group of mice. In conclusion, Ginseng Radix (G), Rehmanniae Radix (R), and Fermented red-ginseng (FRG) are considered to have the potential for development as candidate drugs to control the progression of Alzheimer's disease (AD).

• Journal of the Korea Convergence Society
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• v.7 no.5
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• pp.97-106
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• 2016

This study is to find the converging effects of the Complete Sciences Subjects(CSS) in high school before entrance to University on the scores of Basic Nursing Sciences(BNS) and Major Nursing Subjects(MNS) of 111 senior nursing students of one university. Using the SPSS 19.0, t-test, Pearson's correlation coefficient, multiple and simple linear regression were carried out. The mean scores of the groups that have completed the subjects of Biology I & II, Chemistry I & II were high on Anatomy, Physiology and Pathology. There was a significant difference between the group of completed Biology II and incompleted Biology II on the mean scores of MNS(p<.05). There were positive correlations between the CSS and the scores of BNS and MNS, a moderate correlation between Chemistry II and BNS(r=.303) and a strong correlation between BNS and MNS(r=.796). Explanation rate of CSS on the scores of BNS, and MNS were 18.2%, 8.2% respectively, and on the scores of BNS on MNS was 63.8%. The result of this study can be used in assessing university entrance procedure after repeated study, extended location and more participants, and to use for making pre-school program for the freshmen who didn't CSS.

• Journal of Physiology & Pathology in Korean Medicine
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• v.33 no.2
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• pp.102-108
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• 2019

This study aims to evaluate the anti-inflammatory effect of Coptidis Rhizoma (CR) extract for atopic dermatitis through maintaining skin barrier and regulating Th2 cell differentiation. We divided NC/Nga mice into 3 groups as follows; atopy-like dermatitis induced group with CR treatment (CT, n=10), no treatment group(Ctrl), atopy-like dermatitis elicited group(AE). Atopy-like dermatitis was induced to NC/Nga mice by sensitizing with dermatophagoides farinae(DfE) on 7, 8, 9, 11, 12, and 13th week. After inducing atopic dermatitis, CR extract was administered 20 mg/kg daily for the experimental duration to the CT group. We measured the integrity of lipid layers in the epidermis and Th2 differentiation through immunohistochemical staining against filaggrin, loricrin, IL-4, and IL-13. We also measured the distribution of subcutaneous collagen fibers by the Masson's trichrome staining. Administration of CR significantly inhibited the reduction of lipid layers in the skin that caused atopy. The expression of IL-4, IL-13, each of which is a cytokine secreted by T helper type 2 (Th2) cells, was markedly suppressed in the CT group as compared with AE group (p<0.05). CR treatment also decreased the expression of iNOS, $p-I{\kappa}B$. Atopic dermatitis induced dermatological damage to skin, such as hyperplasia of epithelium, and capillary proliferation was significantly reduced by CR administration. CR effectively inhibited the thinning of the skin barrier and inflammatory responses in atopic dermatitis-induced mice. In particular, it showed anti-inflammatory effects by reducing the expression of IL-4 and IL-13, Th2 cell cytokines, which play a crucial role in development of atopic dermatitis. Therefore, CR can be a good candidate to ameliorate and treat atopic dermatitis.