Browse > Article
http://dx.doi.org/10.15188/kjopp.2019.04.33.2.102

Effect of Coptidis Rhizoma extract on Atopic Dermatitis-like Skin Lesions in NC/Nga Mice  

Jung, A Ram (Department of Pediatrics, School of Korean Medicine, Pusan National University)
Ahn, Sang Hyun (Department of Anatomy, College of Korean Medicine, Semyung University)
Jeong, Han Sol (Division of Applied Medicine, School of Korean Medicine, Pusan National University)
Kim, Ki Bong (Department of Pediatrics, School of Korean Medicine, Pusan National University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.33, no.2, 2019 , pp. 102-108 More about this Journal
Abstract
This study aims to evaluate the anti-inflammatory effect of Coptidis Rhizoma (CR) extract for atopic dermatitis through maintaining skin barrier and regulating Th2 cell differentiation. We divided NC/Nga mice into 3 groups as follows; atopy-like dermatitis induced group with CR treatment (CT, n=10), no treatment group(Ctrl), atopy-like dermatitis elicited group(AE). Atopy-like dermatitis was induced to NC/Nga mice by sensitizing with dermatophagoides farinae(DfE) on 7, 8, 9, 11, 12, and 13th week. After inducing atopic dermatitis, CR extract was administered 20 mg/kg daily for the experimental duration to the CT group. We measured the integrity of lipid layers in the epidermis and Th2 differentiation through immunohistochemical staining against filaggrin, loricrin, IL-4, and IL-13. We also measured the distribution of subcutaneous collagen fibers by the Masson's trichrome staining. Administration of CR significantly inhibited the reduction of lipid layers in the skin that caused atopy. The expression of IL-4, IL-13, each of which is a cytokine secreted by T helper type 2 (Th2) cells, was markedly suppressed in the CT group as compared with AE group (p<0.05). CR treatment also decreased the expression of iNOS, $p-I{\kappa}B$. Atopic dermatitis induced dermatological damage to skin, such as hyperplasia of epithelium, and capillary proliferation was significantly reduced by CR administration. CR effectively inhibited the thinning of the skin barrier and inflammatory responses in atopic dermatitis-induced mice. In particular, it showed anti-inflammatory effects by reducing the expression of IL-4 and IL-13, Th2 cell cytokines, which play a crucial role in development of atopic dermatitis. Therefore, CR can be a good candidate to ameliorate and treat atopic dermatitis.
Keywords
Coptidis Rhizoma; Atopic dermatitis; Lipid barrier; IL-4; IL-13;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Weidinger S, Novak N. Atopic dermatitis. Lancet (London, England). 2016;387(10023):1109-22.   DOI
2 Kim JH. Review Articles : Current Understanding of Atopic Dermatitis Pediatr Allergy Respir Dis. 2004;14(1):12-23.
3 Boguniewicz M. Atopic dermatitis: the updated practice parameter and beyond. Allergy Asthma Proc. 2014;35(6):429-34.   DOI
4 Hengge UR, Ruzicka T, Schwartz RA, Cork MJ. Adverse effects of topical glucocorticosteroids. J Am Acad Dermatol. 2006;54(1):1-15; quiz 6-8.   DOI
5 Yun Y, Choi IW. A treatment utilization of Atopic Dermatitis According to Severity J Korean Med Ophthalmol Otolaryngol Dermatol. 2009;22(1):172-80.
6 Kim BE LD. Epidermal barrier in atopic dermatitis. Immunol Res. 2012;4:12-6.
7 Heratizadeh A. Atopic dermatitis: new evidence on the role of allergic inflammation. Curr Opin Allergy Clin Immunol. 2016;16(5):458-64.   DOI
8 Hogan MB, Peele K, Wilson NW. Skin barrier function and its importance at the start of the atopic march. J Allergy (Cairo). 2012;2012:901940.   DOI
9 Boguniewicz M, Eichenfield LF, Hultsch T. Current management of atopic dermatitis and interruption of the atopic march. J Allergy Clin Immunol. 2003;112(6):140-50.   DOI
10 Lou H, Fu Y, Wang C, Wang Y, Zhang L. [Imbalance between Th1 and Th2 cells in cord blood is influenced by maternal allergic rhinitis and associated with atopic dermatitis during the first two years of life]. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2014;49(5):390-4.
11 Halonen M, Lohman IC, Stern DA, Spangenberg A, Anderson D, Mobley S, et al. Th1/Th2 patterns and balance in cytokine production in the parents and infants of a large birth cohort. Journal of immunology (Baltimore, Md : 1950). 2009;182(5):3285-93.   DOI
12 Im GM, Kim HJ, Jeong WY, Jeong HW. Oriental Medical Approach on the Allergic Disease. Korea J Oriental Physiology & Pathology. 2002;16(5):831-9.
13 Kim HK, Hong SU. The anti-inflammatory effects of Huang-Lyun (Coptidis Rhizoma, CR) on injured tissue after burn elicitation. J Korean Oriental Med. 2011;32(2):1-13.
14 Cha HY, Ahn SH, Cheon JH, Park SY, Kim KB. Hataedock treatment has preventive therapeutic effects for atopic dermatitis through skin barrier protection in Dermatophagoides farinae-induced NC/Nga mice. J Ethnopharmacol. 2017;206:327-36.   DOI
15 Li S, Zhang ZQ, Wu LJ, Zhang XG, Li YD, Wang YY. Understanding ZHENG in traditional Chinese medicine in the context of neuro-endocrine-immune network. IET Syst Biol. 2007;1(1):51-60.   DOI
16 Muluye RA, Bian Y, Alemu PN. Anti-inflammatory and Antimicrobial Effects of Heat-Clearing Chinese Herbs: A Current Review. J Tradit Complement Med. 2014;4(2):93-8.   DOI
17 Korean Medical Pharmacology Text ublication committee. Korean medical pharmacology. Seoul:Shinil Books;2010.116-249.
18 Heo J. Donguibogam. Seoul:Yegang publish;2019. p.2976.
19 Kauppi S, Jokelainen J, Timonen M, Tasanen K, Huilaja L. Adult Patients with Atopic Eczema have a High Burden of Psychiatric Disease: A Finnish Nationwide Registry Study. Acta dermato-venereologica. 2019.
20 Kim HJ, Shin JU, Lee KH. REVIEW ARTICLE : Atopic dermatitis and skin barrier dysfunction Allergy Asthma Immunol Res. 2013;1(1):20-8.
21 Chamlin SL, Kao J, Frieden IJ, Sheu MY, Fowler AJ, Fluhr JW, et al. Ceramide-dominant barrier repair lipids alleviate childhood atopic dermatitis: changes in barrier function provide a sensitive indicator of disease activity. J Am Acad Dermatol. 2002;47(2):198-208.   DOI
22 Kim BE, Leung DY, Boguniewicz M, Howell MD. Loricrin and involucrin expression is down-regulated by Th2 cytokines through STAT-6. Clin Immunol. 2008;126(3):332-7.   DOI
23 Onoue A, Kabashima K, Kobayashi M, Mori T, Tokura Y. Induction of eosinophil- and Th2-attracting epidermal chemokines and cutaneous late-phase reaction in tape-stripped skin. Exp Dermatol. 2009;18(12):1036-43.   DOI
24 Wood LC, Elias PM, Calhoun C, Tsai JC, Grunfeld C, Feingold KR. Barrier disruption stimulates interleukin-1 alpha expression and release from a pre-formed pool in murine epidermis. J Invest Dermatol. 1996;106(3):397-403.   DOI
25 Cornelissen C, Marquardt Y, Czaja K, Wenzel J, Frank J, Luscher-Firzlaff J, et al. IL-31 regulates differentiation and filaggrin expression in human organotypic skin models. J Allergy Clin Immunol. 2012;129(2):426-33.   DOI
26 Lee SH, Jeong SK, Ahn SK. An update of the defensive barrier function of skin. Yonsei Med J. 2006;47:293-306.   DOI
27 Sehra S, Krishnamurthy P, Koh B, Zhou HM, Seymour L, Akhtar N, et al. Increased Th2 activity and diminished skin barrier function cooperate in allergic skin inflammation. Eur J Immunol. 2016;46(11):2609-13.   DOI
28 Kim HJ, Shin JU, Lee KH. Atopic dermatitis and skin barrier dysfunction. Allergy Asthma Respir Dis. 2013;1(1):20-8.   DOI
29 van Smeden J, Bouwstra JA. Stratum Corneum Lipids: Their Role for the Skin Barrier Function in Healthy Subjects and Atopic Dermatitis Patients. Curr Probl Dermatol. 2016;49:8-26.   DOI
30 Armengot-Carbo M, Hernandez-Martin A, Torrelo A. The role of filaggrin in the skin barrier and disease development. Actas Dermosifiliogr. 2015;106(2):86-95.   DOI
31 Brown A, Farmer K, MacDonald L, Kalsheker N, Pritchard D, Haslett C, et al. House dust mite Der p 1 downregulates defenses of the lung by inactivating elastase inhibitors. Am J Respir Cell Mol Biol. 2003;29(3 Pt 1):381-9.   DOI
32 Moya R, Carnes J, Sinovas N, Ramio L, Brazis P, Puigdemont A. Immunoproteomic characterization of a Dermatophagoides farinae extract used in the treatment of canine atopic dermatitis. Vet Immunol Immunopathol. 2016;180:1-8.   DOI
33 Wang P, Wu P, Siegel MI, Egan RW, Billah MM. Interleukin (IL)-10 inhibits nuclear factor kappa B (NF kappa B) activation in human monocytes. IL-10 and IL-4 suppress cytokine synthesis by different mechanisms. J Biol Chem. 1995;270(16):9558-63.   DOI
34 Chae S. Function and Activation of $NF-{\kappa}B$ in Immune System. Korean J Otolaryngol. 2005;48:284-8.
35 Cha HY, Ahn SH, Cheon JH, Park SY, Choi JY, Kim KB. Anti-inflammatory Effects of Hataedock Extracted from Coptidis Rhizoma and Glycyrrhiza Uralensis on Atopic Dermatitis-like Skin Lesions of NC/Nga Mouse. J Int Korean Med. 2015;36(4):486-97.
36 Abdallah WI, Abd El Maksoud RA, Hashad DI, El Azhary NM, Abonayeem SM. Correlation between waist:height ratio and serum nitric oxide level in children with atopic dermatitis. Pediatr Dermatol. 2015;32(3):429-30.   DOI