Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Coptidis Rhizoma extract on Atopic Dermatitis-like Skin Lesions in NC/Nga Mice

황련 추출물의 아토피피부염 유발 생쥐에서 피부손상 완화 효과

  • Jung, A Ram (Department of Pediatrics, School of Korean Medicine, Pusan National University) ;
  • Ahn, Sang Hyun (Department of Anatomy, College of Korean Medicine, Semyung University) ;
  • Jeong, Han Sol (Division of Applied Medicine, School of Korean Medicine, Pusan National University) ;
  • Kim, Ki Bong (Department of Pediatrics, School of Korean Medicine, Pusan National University)
  • 정아람 (부산대학교 한의학전문대학원 한방소아과학교실) ;
  • 안상현 (세명대학교 한의과대학 해부학교실) ;
  • 정한솔 (부산대학교 한의학전문대학원 응용의학부) ;
  • 김기봉 (부산대학교 한의학전문대학원 한방소아과학교실)
  • Received : 2019.03.18
  • Accepted : 2019.04.22
  • Published : 2019.04.25
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Abstract

This study aims to evaluate the anti-inflammatory effect of Coptidis Rhizoma (CR) extract for atopic dermatitis through maintaining skin barrier and regulating Th2 cell differentiation. We divided NC/Nga mice into 3 groups as follows; atopy-like dermatitis induced group with CR treatment (CT, n=10), no treatment group(Ctrl), atopy-like dermatitis elicited group(AE). Atopy-like dermatitis was induced to NC/Nga mice by sensitizing with dermatophagoides farinae(DfE) on 7, 8, 9, 11, 12, and 13th week. After inducing atopic dermatitis, CR extract was administered 20 mg/kg daily for the experimental duration to the CT group. We measured the integrity of lipid layers in the epidermis and Th2 differentiation through immunohistochemical staining against filaggrin, loricrin, IL-4, and IL-13. We also measured the distribution of subcutaneous collagen fibers by the Masson's trichrome staining. Administration of CR significantly inhibited the reduction of lipid layers in the skin that caused atopy. The expression of IL-4, IL-13, each of which is a cytokine secreted by T helper type 2 (Th2) cells, was markedly suppressed in the CT group as compared with AE group (p<0.05). CR treatment also decreased the expression of iNOS, $p-I{\kappa}B$. Atopic dermatitis induced dermatological damage to skin, such as hyperplasia of epithelium, and capillary proliferation was significantly reduced by CR administration. CR effectively inhibited the thinning of the skin barrier and inflammatory responses in atopic dermatitis-induced mice. In particular, it showed anti-inflammatory effects by reducing the expression of IL-4 and IL-13, Th2 cell cytokines, which play a crucial role in development of atopic dermatitis. Therefore, CR can be a good candidate to ameliorate and treat atopic dermatitis.

Keywords

References

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