• Title/Summary/Keyword: 항진균성 활성도

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An Antifungal Subatance, 2,4-Diacetylphloroglucinol Produced from Antagonistic Bacterium Pseudo-monas fluorescens 2112 Against Phytophthora capsici (Phytophthora capsici를 길항하는 Pseudononas fluorescens 2112가 생산하는 항진균 항생물질 2,4-diacetylphloroglucinol)

  • 이은탁;김상달
    • Microbiology and Biotechnology Letters
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    • v.29 no.1
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    • pp.37-42
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    • 2001
  • An antifungal substance was purified from culture broth of Pseudomonas flulorescens 2112 that showed a broad-spectrum antagonistic activity against various phytopathogenic fungi including capsici. The substance was identified as 2,4-diacetylphloro-glucinol basd on NMR analysis. The 2,4-diacetylphloroglcinol showed antibiotic activity in broad acidic range from pH 1.0 to pH 9.0. About 83% of initial activity was remained after incubation for 30min ar $60^{\circ}C$, however, the activity was dropped up to 50% after 30 min incubation in $80^{\circ}C$. When the nucleotides of P. capsici treated with 2,4-diacetylphloroglucinol were labeled with[$^{3}$ H]-Adenin, the newly synthesized and radioactive-labeled RNA was significantly reduced than those of untreated P. capsici. indicating that the 2,4-diacetylphloroglucinol inhibits RNA synthesis.

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Isolation and Antifungar Activity of Bacillus ehimensis YJ-37 as Antagonistic against Vegetables Damping-off Fungi (채소류 모잘록병균에 길항하는 Bacillus ehimensis YJ-37의 선발과 항진균성)

  • 주길재;김진호;강상재
    • Journal of Life Science
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    • v.12 no.2
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    • pp.200-207
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    • 2002
  • This study was carried out to isolate of antagonistic bacterium against Pythium ultimum and Rhizoctonia solani AG-4, causal pathogens of vegetables damping-off. Total of 600 strains were isolated from soil and plait roots. The isolates were screened for antagonism against Pythium ultimum and Rhizoctonia solani AG-4. One strain, named YJ-37, was sellected for detained study among those microoganisms screened. It was identified as Bacillus ehimensis based on morphological and physiological characterisitics according to the Bergey's mannual of systematic bacteriology, Sherlock system of Microbial ID Inc. and 16S rDNA sequences methods. Furthermore Bacillus ehimensis YJ-37 showed antifungal activities against Alternaria altrata, Collectotrichum gloeosporioides, Didymella bryoniae, Fusarium moniliforme, Fusarium oxysporum, F. oxysporum cucumerinum, F. oxysporum niveum, Gloeosporium sp., Glomerella sp., G. cingulata, G. lagenaria, Penicillium digitatum, P. italicum, Phytophthora capsici, Sclerotinia sclerotiorum, and Stemprhylium solani.

Antimicrobial Activities of Urushiol and Urushiol Derivatives (우루시올 및 우루시올 유도체의 항균 활성)

  • Kim, Jin-Chul;Ahn, Jeong-Keun;Ko, Soo-Young;Choi, Young-Hoon;Kim, Do-Hyun;Lee, Tae-Yong
    • Clean Technology
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    • v.13 no.1 s.36
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    • pp.22-27
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    • 2007
  • Pacquer traditionally has been used to varnish. Many reports have revealed that lacquer has durability and antimicrobial activities. Therefore, we expect that lacquer will be used as a good antifouling agent to solve the environmental problem. Here we chemically synthesized urushiol, a major component in lacquer and two urushiol derivatives, urusiol regioisomer and cardanol. We also analyzed the antimicrobial activities of these molecules to examine the inhibitory effect on the formation of the biofilms. Our results showed that synthesized urushiol and its derivatives have strong antifungal activities. Urushiol also exhibited inhibitory effect on the growth of gram positive bacteria specifically. However urushiol derivatives have low antibacterial activities.

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Antifungal Activity of Bacillus vallismortis 1A against Phytopathogen (식물병원균에 대한 Bacillus vallismortis 1A 균주의 항진균 활성)

  • Lee, Mi-Hye;Kim, Soo-Jin;Lee, Chang-Muk;Jang, Jae-Seon;Chang, Hai-Joong;Park, Min-Seon;Koo, Bon-Sung;Yoon, Sang-Hong;Yeo, Yun-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.5
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    • pp.362-368
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    • 2008
  • In order to isolate novel oligotrophic bacteria exhibiting antifungal activities, soils were collected from pepper-cultivated fields of Yeongyang, Jecheon, Nonsan, Eumsong and Goesan area in Korea. From soils in pepper cultivated area, a total of 9,354 strains were isolated as oligotrophic bacteria by the R2A dilution method. Among 9,354 oligotrohic bacteria candidates, 1A strain was selected by screening against Phytophthora capsici causing phytophthora blight of hot pepper in the greenhouse and field. The strain was identified as Bacillus vallismortis based on its 16S rDNA sequence and key characteristics as compared with those of authentic cultures of B. vallismortis(KACC 12149) and B. mojavensis(KACC 12096). The strain showed broad spectrum of antibiotic activity in vitro test, as revealed in its strong inhibitory activity to the genera Phytophthora, Collectotrichum, Botrytis and Fusarium, but not to Rhizoctonia and Magnaporthe. In pot experiments, infection rate of hot pepper in the non-treated pots was about 89%, while it was only 29% in the pots treated with 1A strain. The result indicated B. vallismortis 1A is a potential biocontrol agent for phytophthora blight of hot pepper

Structural Identification of $Siderophore_{AH18}$ from Bacillus subtilis AH18, a Biocontrol agent of Phytophthora Blight Disease in Red-pepper (Bacillus subtilis AH18의 고추역병 방제능과 $Siderophore_{AH18}$의 구조분석)

  • Woo, Sang-Min;Kim, Sang-Dal
    • Microbiology and Biotechnology Letters
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    • v.36 no.4
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    • pp.326-335
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    • 2008
  • The siderophore ($siderophore_{AH18}$) of Bacillus subtilis AR18 was determined to be one of catechol type and purified by using Amberlite XAD-2, Sephadex LR-20 chromatography, and reversed-phase RPLC. The $Siderophore_{AH18}$ was identified bacillibactin with its structure by GC-MS, $^1H$-NMR, and $^{13}C$-NMR. $Siderophore_{AH18}$ (bacillibactin) had been confirmed its molecular weight of 883 and chemical structure of $(2,3-dihydroxybenzoate-glycine-threonine)_3$. Purified $siderophore_{AH18}$ showed strong biocontrol ability towards the spore of Phytophthora capsici on PDA and able to effectively suppress (55%) P. capsici causing red-pepper blight in the pot in vivo test.

Functional Genomic Analysis of Bacillus thuringiensis C25 Reveals the Potential Genes Regulating Antifungal Activity against Rosellinia necatrix (Bacillus thuringiensis C25의 흰날개무늬병 Rosellinia necatrix에 대한 항진균 활성에 관여하는 유전자 특성 및 기능 유전체학적 연구)

  • Kim, Kangmin;Lee, Hwa-Yong;Bae, Wonsil;Cho, Min;Ryu, Hojin
    • The Korean Journal of Mycology
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    • v.47 no.4
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    • pp.417-425
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    • 2019
  • Biocontrol agents (BCAs) are widely used to protect plants from diverse biotic and abiotic stresses in agricultural and ecological fields. Among the various microbes, many subspecies of the gram-positive genus, Bacillus, have been successfully industrialized as eco-friendly biological pesticides and fertilizers. In the current study, we demonstrated that Bacillus thuringiensis C25 exhibited antagonistic effects on the mycelial growth of Rosellinia necatrix, a fungal phytopathogen. Scanning electron microscopy analysis revealed that B. thuringiensis C25 degraded the cell wall structures of R. necatrix mycelia. In the functional genomic analysis of B. thuringiensis C25, we annotated 5,683 genes and selected the gene sets that potentially encoded fungal cell wall degrading enzymes (CWDEs). The growth inhibition effects on R. necatrix were highly correlated with the transcriptional activity of the mycelial cell wall degrading genes of B. thuringiensis C25. The transcript levels of CWDEs, including CshiA, B, and Glycos_transf_2 genes in B. thuringiensis C25, were enhanced following co-cultivation with R. necatrix. In conclusion, our study suggested that B. thuringiensis C25 could serve as a suitable candidate for controlling R. necatrix and could facilitate elucidating the mechanisms underlying the antifungal activities of BCAs against phytopathogens.

Identification of the Marine Microorganisms Producing Bioactives -I. Isolation and Cultural Conditions of the Marine Actinomycetes No. 101 Producing Antimicrobial compounds- (생리활성물질을 생성하는 해양미생물의 동정 -I. 항미생물 물질을 생산하는 해양방선균 분리균주 No. 101의 분리 및 배양조건-)

  • CHOI Jong-Duck;PARK Uk-Yeon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.26 no.4
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    • pp.305-311
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    • 1993
  • Marine organisms were investigated to identify the marine actinomycetes that produced noble bioactive compounds. Microorganism counts range from $2.1{\times}10^3\;to\;1.2{\times}10\;CFU/g$ of marine organisms. Actinomycetes constituted 0.01 to $0.5\%$ of culturable microbial community. We identified the marine actinomycetes that produced novel bioactive compounds. During the course of screening for bioactives from the marine microorganisms, we found that the strain in sponge had antimicrobial activities. From the morphological, cultural and various physiological characteristics, this strain was identified for Actinomycetes No. 101. The optimal compositions of culture medium for Actinomycetes No. 101 were starch 30g/l as carbon source, casamino acid 10g/l as nitrogen source. The optimal pH of medium and fermentation temperature were $6.5{\sim}7.0$ and $30^{\circ}C$, respectively. Fermentation has been conducted in the marine broth at $30^{\circ}C$ for 72 hour. The yield of fermentation got about 3g as dry weight(per liter of broth). The distribution of antimicrobial activity of Actinomycetes No. 101 was screened by paper disc. The extract of cultured cell and broth inhibited the growth of Staphylococcus aureus and Bacillus subtilis, but the inhibition action was week against yeast and mold.

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Selection and Antagonistic Mechanism of Bacillus thuringiensis BK4 against Fusarium Wilt Disease of Tomato (토마토시들음병의 생물학적 방제를 위한 토착길항세균 Bacillus thuringiensis BK4의 선발과 길항기작)

  • Jung Hee-Kyoung;Kim Jin-Rack;Kim Bo-Kum;Yu Tae-Shik;Kim Sang-Dal
    • Microbiology and Biotechnology Letters
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    • v.33 no.3
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    • pp.194-199
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    • 2005
  • In oder to select an antifungal substance-producing antagonistic bacterium against Fusarium oxysporum casuing fusarium wilt on tomato, strain BK4 was isolated from local soil of Gyeoungbuk and was identified as Bacillus thuringiensis by 16s rDNA analysis, biochemical test, and Mcirolog TM 3.0 System. The antibiotic of B. thuringiensis BK 4 was highly produced at $30^{\circ}C$ in nutrient broth (pH 9.0). The crude antibiotic was even stable at $121^{\circ}C$ and more stable at slight alkalic condition than acid condition. It was also remained $50{\%}$ activity at pH 3.0. B. thuringiensis BK4 showed the inhibition of spore germination and the biocontrol ability against F. oxysporum causing fusarium wilt of tomato in vivo test. According to these results, B. thuringiensis BK4 was enough to use with a microbial agent for biocontrol against fusarium wilt.

Determination of Siderophore from Bacillus Mojavensis Using Liquid Chromatography quadrupole Time-of-flight Tandem Mass Spectrometry (액체크로마토그래피-사중극 비행시간형 탠덤질량분석기를 이용한 Bacillus mojavensis 균주 속 사이드로포어 규명)

  • Cheon, Hae In;Yeo, Mi Seon;Kim, Kang Min;Kang, Jae Seon;Pyo, Jaesung
    • Journal of Life Science
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    • v.29 no.2
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    • pp.198-201
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    • 2019
  • Recently, it has been reported that Bacillus mojavensis possesses antifungal properties and plant growth-promoting activities, which are similar to the characteristics of siderophore. In this study, the siderophore produced by B. mojavensis was assessed using a solid phase extraction (SPE) cartridge and liquid chromatography quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS). After B. mojavensis was incubated in phenol medium for 16 hr and lyophilized, the sample was dissolved in water and loaded to an SPE cartridge to remove interferences. The cartridge was washed with 5% methanol in water and eluted with 2% formic acid in methanol sequentially. The eluted solution was evaporated under a stream of nitrogen gas and reconstituted in methanol. The reconstituted sample was filtered, and $1{\mu}l$ of the sample was assessed using Q-TOF MS/MS. The mass spectrometer was operated using the positive electrospray ionization mode. Based on the mass spectrum and tandem mass spectrum, the siderophore produced by B. mojavensis was bacillibactin, one of the catechol types of siderophore with a molecular weight of 882.2556. This siderophore analysis could provide a justification for the study of B. mojavensis as a functional food and for pharmaceutical applications.

Development of Antimicrobial Dye for Natural Dyeing using Natural Substances (천연물을 이용한 항균성 염료의 개발)

  • Yu, Young-Eun;Park, Eun-Young;Jung, Dae-Hwa;Byun, Sung-Hui;Kim, Sang-Chan;Park, Sung-Min
    • Microbiology and Biotechnology Letters
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    • v.38 no.1
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    • pp.32-39
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    • 2010
  • This study was carried out to investigate the usefulness of the natural antimicrobial substances for natural dyeing. Antimicrobial activity of natural substances, extracted by water, ethanol, methanol and ethyl acetate, were shown over 20 mm by Rheum palmatum, Caesalpinia sappan, Prunus mume, Schizandra chinensis, Rhus javanica, and Coptis japonica. Especially, water and methanol extract of C. japonica were shown strong antimicrobial activity against all investigated strains. Minimum inhibition concentration of C. japonica water extract was investigated 110 mg/mL for Staphylococcus aureus ATCC 6538. Investigated natural substances were not only shown strong antimicrobial activity for natural dyeing but also might be used other industries.