Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
권호 표지

Antifungal Activity of Bacillus vallismortis 1A against Phytopathogen

식물병원균에 대한 Bacillus vallismortis 1A 균주의 항진균 활성

  • Lee, Mi-Hye (Dept.of Technical Support, Yeojugun Agricultural Technology Center) ;
  • Kim, Soo-Jin (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Chang-Muk (Dept. of Agriculrural Bio-resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Jang, Jae-Seon (Dept. of Food and Nutrition, Gachon University of Medicine and Science) ;
  • Chang, Hai-Joong (Dept.of Technical Support, Yeojugun Agricultural Technology Center) ;
  • Park, Min-Seon (Dept.of Biochemistry and Molecular Biology, Ajou University School of Medicine) ;
  • Koo, Bon-Sung (Dept. of Korean Food Research for Globalization, National Academy of Agricultural Science, Rural Development Administration) ;
  • Yoon, Sang-Hong (Dept. of Agriculrural Bio-resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Yeo, Yun-Soo (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration)
  • 이미혜 (여주군 농업기술센터 기술 지원과) ;
  • 김수진 (국립농업과학원 농업유전자원센타) ;
  • 이창묵 (국립농업과학원 농업생명자원부) ;
  • 장재선 (가천의과학대학교 식품영양학과) ;
  • 장해중 (여주군 농업기술센터 기술 지원과) ;
  • 박민선 (아주대학교 생화학교실) ;
  • 구본성 (국립농업과학원 한식세계화연구단) ;
  • 윤상홍 (국립농업과학원 농업생명자원부) ;
  • 여윤수 (국립농업과학원 농업유전자원센타)
  • Received : 2008.09.19
  • Accepted : 2008.10.14
  • Published : 2008.10.30
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Abstract

In order to isolate novel oligotrophic bacteria exhibiting antifungal activities, soils were collected from pepper-cultivated fields of Yeongyang, Jecheon, Nonsan, Eumsong and Goesan area in Korea. From soils in pepper cultivated area, a total of 9,354 strains were isolated as oligotrophic bacteria by the R2A dilution method. Among 9,354 oligotrohic bacteria candidates, 1A strain was selected by screening against Phytophthora capsici causing phytophthora blight of hot pepper in the greenhouse and field. The strain was identified as Bacillus vallismortis based on its 16S rDNA sequence and key characteristics as compared with those of authentic cultures of B. vallismortis(KACC 12149) and B. mojavensis(KACC 12096). The strain showed broad spectrum of antibiotic activity in vitro test, as revealed in its strong inhibitory activity to the genera Phytophthora, Collectotrichum, Botrytis and Fusarium, but not to Rhizoctonia and Magnaporthe. In pot experiments, infection rate of hot pepper in the non-treated pots was about 89%, while it was only 29% in the pots treated with 1A strain. The result indicated B. vallismortis 1A is a potential biocontrol agent for phytophthora blight of hot pepper

항진균성 활성을 가지는 저영양세균을 분리하기 위하여 토양시료는 경북 영양, 충북 제천, 충북 음성, 충북 괴산, 충남 논산 등지의 고추 밭에서 수집하였고 R2A배지를 이용하여 평판희석법으로 9,354여 균주의 저영양세균을 분리하였다. 분리된 저영양세균중 고추역병에 강한 활성을 가지는 1A 균주를 선발 하였으며 16S rDNA 와 표준균주(B. vallismortis, B. mojavensis)를 이용한 생리, 생화학적 실험으로 B. vallismortis로 최종동정 되었다. 1A균주는 Magnaporthe 균을 제외하고 Phytophthora, Collectotrichum, Botrytis, Fusarium 균등에서 폭넓게 강한 활성을 나타내었다. 고추 유묘검정에서 대조구가 89%정도의 발병율을 보인 반면에 1A 처리구에서는 29%의 발병율을 나타내어 고추역병의 길항균으로써의 미생물제제 가능성이 있는 것으로 판단된다.

Keywords

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