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http://dx.doi.org/10.4489/KJM.20190046

Functional Genomic Analysis of Bacillus thuringiensis C25 Reveals the Potential Genes Regulating Antifungal Activity against Rosellinia necatrix  

Kim, Kangmin (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University)
Lee, Hwa-Yong (Department of Biology, Chungbuk National University)
Bae, Wonsil (Department of Biology, Chungbuk National University)
Cho, Min (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University)
Ryu, Hojin (Department of Biology, Chungbuk National University)
Publication Information
The Korean Journal of Mycology / v.47, no.4, 2019 , pp. 417-425 More about this Journal
Abstract
Biocontrol agents (BCAs) are widely used to protect plants from diverse biotic and abiotic stresses in agricultural and ecological fields. Among the various microbes, many subspecies of the gram-positive genus, Bacillus, have been successfully industrialized as eco-friendly biological pesticides and fertilizers. In the current study, we demonstrated that Bacillus thuringiensis C25 exhibited antagonistic effects on the mycelial growth of Rosellinia necatrix, a fungal phytopathogen. Scanning electron microscopy analysis revealed that B. thuringiensis C25 degraded the cell wall structures of R. necatrix mycelia. In the functional genomic analysis of B. thuringiensis C25, we annotated 5,683 genes and selected the gene sets that potentially encoded fungal cell wall degrading enzymes (CWDEs). The growth inhibition effects on R. necatrix were highly correlated with the transcriptional activity of the mycelial cell wall degrading genes of B. thuringiensis C25. The transcript levels of CWDEs, including CshiA, B, and Glycos_transf_2 genes in B. thuringiensis C25, were enhanced following co-cultivation with R. necatrix. In conclusion, our study suggested that B. thuringiensis C25 could serve as a suitable candidate for controlling R. necatrix and could facilitate elucidating the mechanisms underlying the antifungal activities of BCAs against phytopathogens.
Keywords
Bacillus thuringiensis C25; Biological control; Cell wall degrading enzymes; Gene annotation; Rosellinia necatrix;
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