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Antifungal Activity of Bacillus vallismortis 1A against Phytopathogen  

Lee, Mi-Hye (Dept.of Technical Support, Yeojugun Agricultural Technology Center)
Kim, Soo-Jin (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration)
Lee, Chang-Muk (Dept. of Agriculrural Bio-resources, National Academy of Agricultural Science, Rural Development Administration)
Jang, Jae-Seon (Dept. of Food and Nutrition, Gachon University of Medicine and Science)
Chang, Hai-Joong (Dept.of Technical Support, Yeojugun Agricultural Technology Center)
Park, Min-Seon (Dept.of Biochemistry and Molecular Biology, Ajou University School of Medicine)
Koo, Bon-Sung (Dept. of Korean Food Research for Globalization, National Academy of Agricultural Science, Rural Development Administration)
Yoon, Sang-Hong (Dept. of Agriculrural Bio-resources, National Academy of Agricultural Science, Rural Development Administration)
Yeo, Yun-Soo (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration)
Publication Information
Korean Journal of Soil Science and Fertilizer / v.41, no.5, 2008 , pp. 362-368 More about this Journal
Abstract
In order to isolate novel oligotrophic bacteria exhibiting antifungal activities, soils were collected from pepper-cultivated fields of Yeongyang, Jecheon, Nonsan, Eumsong and Goesan area in Korea. From soils in pepper cultivated area, a total of 9,354 strains were isolated as oligotrophic bacteria by the R2A dilution method. Among 9,354 oligotrohic bacteria candidates, 1A strain was selected by screening against Phytophthora capsici causing phytophthora blight of hot pepper in the greenhouse and field. The strain was identified as Bacillus vallismortis based on its 16S rDNA sequence and key characteristics as compared with those of authentic cultures of B. vallismortis(KACC 12149) and B. mojavensis(KACC 12096). The strain showed broad spectrum of antibiotic activity in vitro test, as revealed in its strong inhibitory activity to the genera Phytophthora, Collectotrichum, Botrytis and Fusarium, but not to Rhizoctonia and Magnaporthe. In pot experiments, infection rate of hot pepper in the non-treated pots was about 89%, while it was only 29% in the pots treated with 1A strain. The result indicated B. vallismortis 1A is a potential biocontrol agent for phytophthora blight of hot pepper
Keywords
Biological control; Oligotrophic bacteria; Phytophthora blight;
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