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Selection and Antagonistic Mechanism of Bacillus thuringiensis BK4 against Fusarium Wilt Disease of Tomato  

Jung Hee-Kyoung (Department of Applied Microbiology, College of Natural Resources, Yeungnam University)
Kim Jin-Rack (Department of Applied Microbiology, College of Natural Resources, Yeungnam University)
Kim Bo-Kum (Department of Applied Microbiology, College of Natural Resources, Yeungnam University)
Yu Tae-Shik (Department of Microbiology, Keimyung University)
Kim Sang-Dal (Department of Applied Microbiology, College of Natural Resources, Yeungnam University)
Publication Information
Microbiology and Biotechnology Letters / v.33, no.3, 2005 , pp. 194-199 More about this Journal
Abstract
In oder to select an antifungal substance-producing antagonistic bacterium against Fusarium oxysporum casuing fusarium wilt on tomato, strain BK4 was isolated from local soil of Gyeoungbuk and was identified as Bacillus thuringiensis by 16s rDNA analysis, biochemical test, and Mcirolog TM 3.0 System. The antibiotic of B. thuringiensis BK 4 was highly produced at $30^{\circ}C$ in nutrient broth (pH 9.0). The crude antibiotic was even stable at $121^{\circ}C$ and more stable at slight alkalic condition than acid condition. It was also remained $50{\%}$ activity at pH 3.0. B. thuringiensis BK4 showed the inhibition of spore germination and the biocontrol ability against F. oxysporum causing fusarium wilt of tomato in vivo test. According to these results, B. thuringiensis BK4 was enough to use with a microbial agent for biocontrol against fusarium wilt.
Keywords
Biocontrol; antifungal antibiotic; Fusarium oxysporum; Bacillus thuringiensis;
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